专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5200311A Method of determination of pesticides by radiobiochemistry 失效
标题翻译：通过放射生物化学测定农药的方法
公开(公告)号：US5200311A
公开(公告)日：1993-04-06
申请号：US556952
申请日：1990-07-19
申请人： Stanley E. Charm , Shlomo Capua , Eliezer Zomer
发明人： Stanley E. Charm , Shlomo Capua , Eliezer Zomer
IPC分类号： G01N33/567 , G01N33/60
CPC分类号： G01N33/567 , G01N33/60 , Y10S436/804
摘要： A biochemical system in the determination of pesticides which is able to detect and measure the presence of parts per billion levels of different pesticides particularly in foodstuffs. The method employs insect tissues that contain specific binders or enzymes sensitive to various groups of pesticides. The sample or sample extract containing pesticides, radiolabeled pesticides and/or radiolabeled pesticide analogs and/or radiolabeled substrates is exposed to insect tissues containing receptors or enzymes. The pesticide competes with the radioactive tracers for the binders or the enzymes in the insect tissues. Residual enzyme activity or bound pesticides are measured. The extent of binding or enzyme activity may be inversely proportional, under standardized conditions, to the amount of pesticide in the sample.
摘要翻译：一种生化系统，用于确定农药，能够检测和测量特别是在食品中存在十亿分之几的不同农药。该方法使用含有对各种农药敏感的特异性结合剂或酶的昆虫组织。含有农药，放射性标记的农药和/或放射性标记的农药类似物和/或放射性标记的底物的样品或样品提取物暴露于含有受体或酶的昆虫组织。杀虫剂与放射性示踪剂竞争昆虫组织中的结合物或酶。测量残留的酶活性或结合的农药。在标准条件下，结合或酶活性的程度可能与样品中农药的量成反比。

2. 发明授权

US5374534A Method of preparing D-luciferin derivatives 失效
标题翻译： D-萤光素衍生物的制备方法
公开(公告)号：US5374534A
公开(公告)日：1994-12-20
申请号：US62315
申请日：1993-05-14
申请人： Eliezer Zomer , Steven Saul , Stanley E. Charm
发明人： Eliezer Zomer , Steven Saul , Stanley E. Charm
IPC分类号： C07D277/64 , C12Q1/66 , G01N33/567 , G01N33/60 , A61K37/02 , C07D277/62 , C12Q1/00
CPC分类号： G01N33/567 , C07D277/64 , C12Q1/66 , G01N33/60
摘要： A method of preparing a 6-substituted D-luciferin ester suitable for use in the determination of pesticides by a bioluminescence method. The 6-substituted D-luciferin esters, such as 6-D-luciferin acetate, are prepared in a liquid solvent containing N-acetyl imidazole with D-luciferin and recovering as the reaction product 6-D-luciferin acetate.
摘要翻译：一种制备适合用于通过生物发光法测定农药的6-取代的D-荧光素酯的方法。 6-取代的D-荧光素酯，如6-D-萤光素乙酸酯，在含有N-乙酰基咪唑与D-荧光素的液体溶剂中制备，作为反应产物6-D-荧光素乙酸酯回收。

3. 发明授权

US5728542A Disposable test kit apparatus and method for bacteria 失效
标题翻译：一次性测试试剂盒和细菌方法
公开(公告)号：US5728542A
公开(公告)日：1998-03-17
申请号：US614813
申请日：1996-03-08
申请人： Stanley E. Charm , Max Gandman , Eliezer Zomer
发明人： Stanley E. Charm , Max Gandman , Eliezer Zomer
IPC分类号： C12M1/00 , C12Q1/06 , C12Q1/10 , A61L2/16
CPC分类号： C12Q1/10 , C12Q1/06 , G01N2333/245
摘要： A safe, disposable test kit apparatus for the detection of pathogens in a test sample, such as coliform bacteria and E. coli. The apparatus comprises a transparent plastic bag divided into a sample compartment to receive a test sample such as water and a media compartment having a powdered growth medium with an indicator for the pathogens. The test kit apparatus includes a sanitizer compound or composition in a separate compartment to be introduced into the sample and media compartment after incubation and reading of the test results to provide for the total deactivation of the pathogen grown on the media after incubation thereby permitting the safe disposal of the plastic bag with the ingredients therein.
摘要翻译：一种用于检测测试样品（如大肠杆菌和大肠杆菌）中病原体的安全的一次性检测试剂盒装置。该装置包括分为样品室的透明塑料袋，以接收诸如水的测试样品和具有粉状生长培养基的培养基，其具有用于病原体的指示剂。测试试剂盒装置包括在分离的隔室中的消毒剂化合物或组合物，以在温育和读取测试结果之后引入样品和培养基室中，以提供孵育后在培养基上生长的病原体的总失活，从而允许安全处理塑料袋与其中的成分。

4. 发明授权

US06319466B1 Test device for detecting the presence of a residue analyte in a sample 失效
标题翻译：用于检测样品中残留物分析物的存在的测试装置
公开(公告)号：US06319466B1
公开(公告)日：2001-11-20
申请号：US09118135
申请日：1998-07-16
申请人： Robert J. Markovsky , Cheryl A. Boyer , Stanley E. Charm , Paul R. Donahue , Yael Agi Glickman , Steven J. Saul , Joan L. Scheemaker , Richard T. Skiffington , Shefali B. Trivedi , Eliezer Zomer
发明人： Robert J. Markovsky , Cheryl A. Boyer , Stanley E. Charm , Paul R. Donahue , Yael Agi Glickman , Steven J. Saul , Joan L. Scheemaker , Richard T. Skiffington , Shefali B. Trivedi , Eliezer Zomer
IPC分类号： G01N2178
CPC分类号： G01N33/558 , A23D9/00 , A23K10/30 , A23K20/158 , C11B3/001 , C11B7/00 , C11C1/04 , C11C3/10 , C11C3/123 , C12N9/0083 , C12N15/8216 , C12N15/8222 , C12N15/8234 , C12N15/8247 , G01N33/537 , G01N33/54366 , G01N2201/0221 , Y10S435/805 , Y10S435/97 , Y10T436/10 , Y10T436/105831
摘要： A test device for detecting the presence of a residue analyte in a sample includes a support strip and a sample-absorbing matrix attached to the support strip. The sample-absorbing matrix has a material for absorbing an amount of the sample. The test device also includes a mobile-phase support for holding a mobile-phase composition. The mobile-phase support is attached to the support strip and in contact with the sample-absorbing matrix. A mobile-phase composition is disposed on the mobile-phase support and has a receptor for binding with the analyte. The mobile-phase composition can be carried in the sample. A stationary-phase membrane is attached to the support strip and has a first membrane end in contact with the mobile-phase composition and a second membrane end. The membrane allows lateral capillary flow of the sample from the first membrane end to the second membrane end. A test zone is on the stationary-phase membrane between the first membrane end and second membrane end and having a first binder for binding with an unbound receptor. A control zone is the stationary-phase a membrane between the test zone and second membrane end and has a second binder for binding with an analyte-bound receptor or residual unbound receptor.
摘要翻译：用于检测样品中残留物分析物的存在的测试装置包括支撑条和连接到支撑条的样品吸收基质。样品吸收基质具有用于吸收一定量样品的材料。测试装置还包括用于保持流动相组成的流动相支持。流动相载体连接到支撑条上并与样品吸收基质接触。移动相组合物设置在流动相载体上并具有用于与分析物结合的受体。流动相组合物可以携带在样品中。固定相膜连接到支撑条上并具有与流动相组合物接触的第一膜端和第二膜端。膜允许样品从第一膜端到第二膜端的侧向毛细管流动。测试区位于第一膜端和第二膜端之间的固定相膜上，并具有用于与未结合受体结合的第一粘合剂。控制区是测试区和第二膜端之间的固定相膜，并且具有用于与分析物结合的受体或残留的未结合受体结合的第二粘合剂。

5. 发明授权

US5283180A Bioluminescence method for the determination of pesticides 失效
标题翻译：生物发光法测定农药
公开(公告)号：US5283180A
公开(公告)日：1994-02-01
申请号：US818782
申请日：1992-01-09
申请人： Eliezer Zomer , Steven Saul , Stanley E. Charm
发明人： Eliezer Zomer , Steven Saul , Stanley E. Charm
IPC分类号： C07D277/68 , C07D277/64 , C12Q1/66 , G01N21/76 , G01N33/567 , G01N33/60 , C12Q1/00
CPC分类号： G01N33/567 , C07D277/64 , C12Q1/66 , G01N33/60 , Y10S435/975
摘要： A method for the rapid and sensitive determination of organophosphate and carbamate pesticides is disclosed. The method employs an insect brain preparation having a mixture of receptors or enzymes with sites that interact with the organophosphate and carbamate pesticides. The pesticides alter or reduce brain activities of the insect brain preparation which are inversely correlated with pesticide concentration in a test sample. The activity is measured by employing substrates which upon exposure to the insect brain preparation are structurally altered and a light emission reaction is observed. The substrates used are 6-substituted D-Luciferin esters wherein the D-Luciferin ester in the presence of the insect brain preparation is inhibited during hydrolysis while in the presence of the pesticide to be determined. Further the D-Luciferin ester in the presence of the insect brain preparation reacts to cleave the substituted ester group at the 6-position to produce D-Luciferin for further reaction with ATP and luciferase to produce bioluminescence. The bioluminescence can then be measured and compared with a control in order to determine the concentration of a pesticide in the test sample.
摘要翻译：公开了一种用于快速和灵敏地测定有机磷酸盐和氨基甲酸酯农药的方法。该方法采用具有受体或酶的混合物的昆虫脑制剂与与有机磷酸盐和氨基甲酸酯农药相互作用的位点。农药改变或减少与测试样品中农药浓度成反比关系的昆虫脑制剂的脑活动。活性通过使用底物测量，其在暴露于昆虫脑制备物结构上改变并观察到发光反应。使用的底物是6-取代的D-萤光素酯，其中在昆虫脑制剂存在下的D-萤光素酯在水解期间在待测定的农药的存在下被抑制。此外，在昆虫脑制剂存在下的D-萤光素酯反应以在6位切割取代的酯基，以产生D-萤光素，以进一步与ATP和荧光素酶反应以产生生物发光。然后可以测量生物发光并与对照进行比较，以确定测试样品中农药的浓度。

6. 发明授权

US06475805B1 Method for detection of an analyte 有权
标题翻译：检测分析物的方法
公开(公告)号：US06475805B1
公开(公告)日：2002-11-05
申请号：US09542203
申请日：2000-04-04
申请人： Stanley E. Charm , Richard Skiffington , Robert J. Markovsky , Eliezer Zomer , Steven J. Saul
发明人： Stanley E. Charm , Richard Skiffington , Robert J. Markovsky , Eliezer Zomer , Steven J. Saul
IPC分类号： G01N33543
CPC分类号： G01N33/54366 , G01N33/537 , Y10S435/97 , Y10S435/971 , Y10S436/807 , Y10S436/81
摘要： A test device, system and method, the device composed of an elongated, toothbrush-shaped, transparent, plastic housing and a lateral-flow test strip for the detection of an analyte, such as a beta-lactam in milk, in the housing, the housing having an expansion cavity to receive expanded, liquid-contacted, absorbing material in the test strip, and to control lateral flow rate and times in the test strip.
摘要翻译：一种测试装置，系统和方法，该装置由细长的牙刷状透明的塑料外壳和侧流测试条组成，用于在壳体中检测分析物，例如牛奶中的β-内酰胺，壳体具有膨胀腔以在测试条中接收膨胀的，液体接触的吸收材料，并且控制测试条中的横向流速和时间。

7. 发明授权

US5985675A Test device for detection of an analyte 失效
标题翻译：用于检测分析物的测试装置
公开(公告)号：US5985675A
公开(公告)日：1999-11-16
申请号：US1775
申请日：1997-12-31
申请人： Stanley E. Charm , Richard Skiffington , Robert J. Markovsky , Eliezer Zomer
发明人： Stanley E. Charm , Richard Skiffington , Robert J. Markovsky , Eliezer Zomer
IPC分类号： G01N33/537 , G01N33/543 , G01N37/00 , G01N33/533
CPC分类号： G01N33/54366 , G01N33/537 , Y10S435/97 , Y10S435/971 , Y10S436/807 , Y10S436/81
摘要： A test device, system and method, the device composed of an elongated, toothbrush-shaped, transparent, plastic housing and a lateral-flow test strip for the detection of an analyte, such as a beta-lactam in milk, in the housing, the housing having an expansion cavity to receive expanded, liquid-contacted, absorbing material in the test strip, and to control lateral flow rate and times in the test strip.
摘要翻译：一种测试装置，系统和方法，该装置由细长的牙刷状透明的塑料外壳和侧流测试条组成，用于在壳体中检测分析物，例如牛奶中的β-内酰胺，壳体具有膨胀腔以在测试条中接收膨胀的，液体接触的吸收材料，并且控制测试条中的横向流速和时间。

8. 发明授权

US5374535A Test kit for determination of organophosphate and carbamate pesticides with insect brain material that hydrolyses a 6-substituted d luciferin ester 失效
标题翻译：用于测定有机磷酸盐和氨基甲酸酯农药的测试试剂盒，用于水解6-取代的d荧光素酯的昆虫脑材料
公开(公告)号：US5374535A
公开(公告)日：1994-12-20
申请号：US125935
申请日：1993-09-23
申请人： Eliezer Zomer , Steven Saul , Stanley E. Charm
发明人： Eliezer Zomer , Steven Saul , Stanley E. Charm
IPC分类号： C07D277/68 , C07D277/64 , C12Q1/66 , G01N21/76 , G01N33/567 , G01N33/60 , C12Q1/00 , C12Q1/18 , G01N31/00
CPC分类号： G01N33/567 , C07D277/64 , C12Q1/66 , G01N33/60 , Y10S435/975
摘要： A test kit and a rapid and sensitive method are provided for the multiple detection of organophosphate and carbamate pesticides. The test method employs an insect brain material that hydrolyzes a 6-substituted D luciferin ester, preferably a 6-substituted acetyl D luciferin ester, to produce D luciferin. Hydrolysis is inhibited by the pesticides in a test sample. Luciferase and adenosine triphosphate react with the D luciferin to produce oxyluciferin and emitted bioluminescence. The brain material is initially incubated with a test sample followed by the addition of the ester, luciferase and adenosine triphosphate. The kit contains the brain material, the ester and optionally luciferase and adenosine triphosphate, and may also contain means for incubating, and means for measuring emitted bioluminescence, and a control standard.
摘要翻译：为检测有机磷酸盐和氨基甲酸酯农药提供了一种检测试剂盒和快速灵敏的方法。测试方法采用水解6-取代的D荧光素酯，优选6-取代的乙酰基D荧光素酯的昆虫脑材料以产生D荧光素。水解被测试样品中的农药所抑制。荧光素酶和三磷酸腺苷与D荧光素反应产生氧化荧光素并发射生物发光。脑材料最初与测试样品一起温育，然后加入酯，荧光素酶和三磷酸腺苷。该试剂盒包含脑材料，酯和任选的荧光素酶和三磷酸腺苷，并且还可以含有用于孵育的装置，以及用于测量发射的生物发光的装置和对照标准。

9. 发明授权

US5354663A Microbial inhibition test kit and method 失效
标题翻译：微生物抑制试验盒及方法
公开(公告)号：US5354663A
公开(公告)日：1994-10-11
申请号：US791785
申请日：1991-11-12
申请人： Stanley E. Charm , Eliezer Zomer , Thomas Lieu , Max Gandman , Lea Gandman
发明人： Stanley E. Charm , Eliezer Zomer , Thomas Lieu , Max Gandman , Lea Gandman
IPC分类号： C12M1/00 , C12M1/36 , C12Q1/18 , C12Q1/00 , C12Q1/02 , C12Q1/04
CPC分类号： C12Q1/18 , G01N2035/00366 , G01N2035/00445 , Y10S435/809
摘要： A test kit and method for the detection of antimicrobial drugs at low concentrations in a sample in a test container, such as milk, which includes a Bacillus stearothermophilus (BST) tablet and a medium tablet with nutrients to stress the BST and, optionally, a pH indicator. The method comprises preheating a test sample to destroy natural inhibitors, cooling the sample, adding a BST tablet, rapidly heating to effect synchronization of BST germination, adding a medium tablet, incubating and detecting the presence or absence of the antimicrobial drug. The test apparatus includes optionally a test apparatus for the controlled, sequenced heating and cooling of the test sample.
摘要翻译：用于在测试容器（例如牛奶）中的样品中检测低浓度抗微生物药物的测试试剂盒和方法，其包括嗜热脂肪芽孢杆菌（BST）片剂和具有营养物质的中等片剂以胁迫BST，以及任选地 pH指示剂该方法包括预热试样以破坏天然抑制剂，冷却样品，加入BST片剂，快速加热以实现BST发芽的同步，加入中等片剂，孵育并检测抗微生物药物的存在与否。测试装置可选地包括用于受控的，有序的加热和冷却测试样品的测试装置。

10. 发明授权

US07097983B2 Method for detecting the presence of an analyte in a sample 有权
标题翻译：用于检测样品中分析物的存在的方法
公开(公告)号：US07097983B2
公开(公告)日：2006-08-29
申请号：US09961998
申请日：2001-09-24
申请人： Robert J. Markovsky , Cheryl A. Boyer , Stanley E. Charm , Paul R. Donahue , Yael Agi Glickman , Steven J. Saul , Joan L. Scheemaker , Richard T. Skiffington , Shefali B. Trivedi , Eliezer Zomer
发明人： Robert J. Markovsky , Cheryl A. Boyer , Stanley E. Charm , Paul R. Donahue , Yael Agi Glickman , Steven J. Saul , Joan L. Scheemaker , Richard T. Skiffington , Shefali B. Trivedi , Eliezer Zomer
IPC分类号： G01N33/53 , G01N31/00 , G01N33/558
CPC分类号： G01N33/558 , A23D9/00 , A23K10/30 , A23K20/158 , C11B3/001 , C11B7/00 , C11C1/04 , C11C3/10 , C11C3/123 , C12N9/0083 , C12N15/8216 , C12N15/8222 , C12N15/8234 , C12N15/8247 , G01N33/537 , G01N33/54366 , G01N2201/0221 , Y10S435/805 , Y10S435/97 , Y10T436/10 , Y10T436/105831
摘要： The invention features a method of determining whether one or more members of an analyte family are present in a sample. The method makes use of a test zone binder, e.g., a receptor that can bind one or a plurality of analytes within an analyte family, the analytes family defined by similar structural binding sites. Members of an analyte family can have different detection level requirements and, therefore, additional analyte binders can be employed to adjust test sensitivity for a subset of the analytes in the analyte family individually, so that each analyte can be detected only if it is present in the sample above a predetermined threshold.
摘要翻译：本发明的特征在于确定样品中是否存在分析物族的一个或多个成员的方法。该方法利用测试区粘合剂，例如可以结合分析物家族内的一种或多种分析物的受体，即由相似结构结合位点定义的分析物族。分析物家族的成员可以具有不同的检测水平要求，因此，可以使用其他分析物结合剂来单独分析分析物家族中一部分分析物的测试灵敏度，以便每个分析物只有在存在于样品高于预定阈值。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式