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1. 发明授权

US08163533B2 Methods and compositions for combinatorial-based production of multivalent recombinant antigens 有权
标题翻译：用于组合制备多价重组抗原的方法和组合物
公开(公告)号：US08163533B2
公开(公告)日：2012-04-24
申请号：US11577303
申请日：2005-10-17
申请人： W. Dorsey Stuart , Edward B. Cambareri
发明人： W. Dorsey Stuart , Edward B. Cambareri
IPC分类号： C12N1/15 , A61K39/145
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/523 , A61K2039/70 , C12N2760/16134 , C40B40/10
摘要： The present invention provides methods and compositions for rapidly producing multivalent recombinant vaccines using filamentous fungal heterokaryons. The present invention relies on the use of filamentous fungal heterokaryons that are generated from combinations of two or more parent strains into which recombinant DNA molecules encoding variants of antigens derived from pathogenic organisms have been introduced. The resulting vaccines are multivalent.
摘要翻译：本发明提供用于快速生产使用丝状真菌异核的多价重组疫苗的方法和组合物。本发明依赖于使用由两个或多个亲本菌株的组合产生的丝状真菌异核菌，其中已经引入了编码来自病原生物体的抗原变体的重组DNA分子。所得疫苗是多价的。

2. 发明申请

US20080318274A1 Methods and Compositions for Combinatorial-Based Production of Multivalent Recombinant Antigens 有权
标题翻译：用于组合制备多价重组抗原的方法和组合
公开(公告)号：US20080318274A1
公开(公告)日：2008-12-25
申请号：US11577303
申请日：2005-10-17
申请人： W. Dorsey Stuart , Edward B. Cambareri
发明人： W. Dorsey Stuart , Edward B. Cambareri
IPC分类号： C12N1/15 , C12P21/00
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/523 , A61K2039/70 , C12N2760/16134 , C40B40/10
摘要： The present invention provides methods and compositions for rapidly producing multivalent recombinant vaccines using filamentous fungal heterokaryons. The present invention relies on the use of filamentous fungal heterokaryons that are generated from combinations of two or more parent strains into which recombinant DNA molecules encoding variants of antigens derived from pathogenic organisms have been introduced. The resulting vaccines are multivalent.
摘要翻译：本发明提供用于快速生产使用丝状真菌异核的多价重组疫苗的方法和组合物。本发明依赖于使用由两个或多个亲本菌株的组合产生的丝状真菌异核菌，其中已经引入了编码来自病原生物体的抗原变体的重组DNA分子。所得疫苗是多价的。

3. 发明授权

US07323299B2 Methods for in vivo diversification of single genes 有权
标题翻译：单基因体内多样化方法
公开(公告)号：US07323299B2
公开(公告)日：2008-01-29
申请号：US10239288
申请日：2001-03-20
申请人： Edward B. Cambareri , Eli E. Kato
发明人： Edward B. Cambareri , Eli E. Kato
IPC分类号： C12Q1/68 , G01N33/53 , G01N33/569 , C12N1/21 , C07H21/02 , C12N15/63
CPC分类号： C12N15/102 , C12N15/1027
摘要： Methods and compositions are provided for the efficient in vivo diversification of gene-products in filamentous fungi, starting from (but not limited to) two or more copies of a single gene constituent. The diversification involve use of the Repeat-Induced Point mutation (RIP) process in N. crassa, and other fungi that have analogous mutational processes. The invention takes advantage of the induction of G:C to A:T transition mutations specific to duplicated DNA sequences during the premeiotic dikaryon phase of the life cycle of the fungus. The methods and compositions of the invention can be utilized to generate diversity in target genes, and are proposed for the purpose of altering and generating novel forms and activities of gene-products thus encoded. Duplicated genes may be introduced into the organism and are present either in tandem, or at separate ectopic locations within the genome of the fungus. After crossing the resulting transformant(s), sexual progeny can then be selected which contain the mutated gene, and subsequently screened for a desired product.
摘要翻译：提供了从（但不限于）单个基因成分的两个或多个拷贝开始的丝状真菌中基因产物的有效体内多样化的方法和组合物。多样化包括使用重复诱导点突变（RIP）过程在粗糙沙门氏菌和其他具有类似突变过程的真菌。本发明利用在真菌生命周期的前期生物二核期期间对重复DNA序列特异性的G：C至A：T转换突变的诱导。本发明的方法和组合物可用于产生目标基因的多样性，并提出用于改变和产生由此编码的基因产物的新形式和活性的目的。重复的基因可以被引入生物体并且以串联的方式存在，或者存在于真菌基因组内的分离的异位的位置。在交叉得到的转化体后，可以选择含有突变基因的性后代，随后筛选所需的产物。

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