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1. 发明授权

US08407588B1 Large columnar text file editor 有权
公开(公告)号：US08407588B1
公开(公告)日：2013-03-26
申请号：US12603908
申请日：2009-10-22
申请人： Weigang Hu , Charles Mark Williams
发明人： Weigang Hu , Charles Mark Williams
IPC分类号： G06F17/21
CPC分类号： G06F17/21 , G06F17/24
摘要： The different advantageous embodiments provide a method for loading and editing text files. A text file is identified as input. A determination is made as to whether the text file is a columnar text file. If the text file is columnar, data information is retrieved for the text file. Pre-defined cache settings are identified. A determination is made as to whether the data information retrieved fits within the pre-defined cache settings. If the data information retrieved fits within the pre-defined cache settings, a determination is made as to whether the first row of the text file is a header row in a view. If the first row of the text file is not a header row, the text file is normalized. A view mode is selected for load and edit operations of the text file.

2. 发明授权

US08301417B2 Method for identifying the analytical transitional cross-sections of a rigid transport element 有权
标题翻译：用于识别刚性传输元件的分析过渡横截面的方法
公开(公告)号：US08301417B2
公开(公告)日：2012-10-30
申请号：US12269747
申请日：2008-11-12
申请人： Weigang Hu
发明人： Weigang Hu
IPC分类号： G06F17/50 , G06G7/48
CPC分类号： G06F17/50 , G06F2217/34
摘要： According to a preferred embodiment, a method for identifying the analytical transitional cross-sections of a rigid transport element is disclosed. The method includes providing a 3D model of a rigid transport element, a list of total edges from the 3D model, a list of total faces from the 3D model, and a list of connecting edges from the 3D model, identifying all edges from said list of total edges which have connectivity with any edge in the list of connecting edges and are part of a cross-section, placing the identified edges into a list of cross-section edges, and identifying the analytical cross-section that lies on the plane defined by the edges in said list of cross-section edges.
摘要翻译：根据优选实施例，公开了用于识别刚性传送元件的分析过渡横截面的方法。该方法包括提供刚性传输元件的3D模型，来自3D模型的总边缘列表，来自3D模型的总面部列表以及来自3D模型的连接边缘列表，从所述列表中识别所有边缘的总边缘与连接边缘列表中的任何边缘具有连接性，并且是横截面的一部分，将识别的边缘放置在横截面边缘的列表中，并且识别位于定义的平面上的分析横截面通过所述横截面边缘列表中的边缘。

3. 发明申请

US20100121611A1 Method for Identifying the Analytical Transitional Cross-sections of a Rigid Transport Element 有权
标题翻译：识别刚性运输元件分析过渡截面的方法
公开(公告)号：US20100121611A1
公开(公告)日：2010-05-13
申请号：US12269747
申请日：2008-11-12
申请人： Weigang Hu
发明人： Weigang Hu
IPC分类号： G06F17/50
CPC分类号： G06F17/50 , G06F2217/34
摘要： According to a preferred embodiment, a method for identifying the analytical transitional cross-sections of a rigid transport element is disclosed. The method includes providing a 3D model of a rigid transport element, a list of total edges from the 3D model, a list of total faces from the 3D model, and a list of connecting edges from the 3D model, identifying all edges from said list of total edges which have connectivity with any edge in the list of connecting edges and are part of a cross-section, placing the identified edges into a list of cross-section edges, and identifying the analytical cross-section that lies on the plane defined by the edges in said list of cross-section edges.
摘要翻译：根据优选实施例，公开了用于识别刚性传送元件的分析过渡横截面的方法。该方法包括提供刚性传输元件的3D模型，来自3D模型的总边缘列表，来自3D模型的总面部列表以及来自3D模型的连接边缘列表，从所述列表中识别所有边缘的总边缘与连接边缘列表中的任何边缘具有连接性，并且是横截面的一部分，将识别的边缘放置在横截面边缘的列表中，并且识别位于定义的平面上的分析横截面通过所述横截面边缘列表中的边缘。

4. 发明授权

US07622111B2 Fusion protein of human IgG1 heavy chain constant region and scFv antibody against equine encephalitis virus 失效
标题翻译：人IgG1重链恒定区的融合蛋白和针对马脑炎病毒的scFv抗体
公开(公告)号：US07622111B2
公开(公告)日：2009-11-24
申请号：US10378832
申请日：2003-03-05
申请人： Leslie P. Nagata , R. Elaine Fulton , Weigang Hu , Azhar Z. Alvi
发明人： Leslie P. Nagata , R. Elaine Fulton , Weigang Hu , Azhar Z. Alvi
IPC分类号： A61K39/42
CPC分类号： C07H21/04 , C07K16/1081 , C07K2317/21 , C07K2317/52 , C07K2317/622 , C07K2319/00
摘要： Construction of a recombinant gene fusion encoding a human IgG1 heavy chain constant region and a single-chain variable fragment antibody of 1A4A1 monoclonal antibody is disclosed. The recombinant antibody of the present invention confers human immune effector functions on murine antibodies. After expression in bacteria as inclusion bodies, the recombinant antibody was purified and refolded in vitro. The recombinant soluble antibody retains high antigen-binding affinity to VEE and possesses some human IgG crystallizable fragment domain functions. On non-reducing gel electrophoresis analysis, disulfide bond formation was found in the hinge region of the recombinant antibody. The present invention shows that the recombinant antibody is in a native, functionally active form and it provides the basis to characterize the recombinant antibody for efficacy in vivo.
摘要翻译：公开了编码人IgG1重链恒定区的重组基因融合物和1A4A1单克隆抗体的单链可变片段抗体的构建。本发明的重组抗体赋予人抗体上的人免疫效应子功能。在细菌中作为包涵体表达后，重组抗体在体外纯化和重折叠。重组可溶性抗体对VEE具有高抗原结合亲和力，并且具有一些人IgG可结晶的片段结构域功能。在非还原凝胶电泳分析中，在重组抗体的铰链区发现二硫键形成。本发明显示重组抗体是天然的功能活性形式，并且为体内重组抗体的功能表征提供了基础。

5. 发明授权

US07527796B2 Genetic engineering of streptavidin-binding peptide tagged single-chain variable fragment antibody to Venezuelan equine encephalitis virus 失效
标题翻译：针对委内瑞兰马脑炎病毒的链霉抗生物素蛋白结合肽标记的单链可变片段抗体的遗传工程
公开(公告)号：US07527796B2
公开(公告)日：2009-05-05
申请号：US10784305
申请日：2004-02-24
申请人： R. Elaine Fulton , Leslie P. Nagata , Azhar Z. Alvi , Weigang Hu
发明人： R. Elaine Fulton , Leslie P. Nagata , Azhar Z. Alvi , Weigang Hu
IPC分类号： A61K39/00 , A61K39/42
CPC分类号： C07K16/1081 , C07K2317/622 , C07K2319/22
摘要： A recombinant gene encoding a single-chain variable fragment (scFv) antibody against Venezuelan equine encephalitis virus (VEE) being cloned into a prokaryotic T7 RNA polymerase-regulated expression vector was disclosed. A streptavidin-binding peptide (SBP) sequence fused to a 6His tag is then attached downstream to the scFv gene. The recombinant fusion protein is expressed in bacteria and then purified by immobilized metal affinity chromatography. ELISA and Western blotting results revealed that the fusion protein not only retained VEE antigen binding and specificity properties similar to those of its parent native monoclonal antibody, but also possessed streptavidin-binding activity. This discovery obviates the need for chemical biotinylation of antibodies and the risk associated with antibody denaturation and provides a stable and reproducible reagent for rapid and efficient immunoassay of VEE.
摘要翻译：公开了克隆到原核T7RNA聚合酶调节表达载体中的编码针对委内瑞兰马脑炎病毒（VEE）的单链可变片段（scFv）抗体的重组基因。然后将与6His标签融合的链霉抗生物素蛋白结合肽（SBP）序列连接到scFv基因的下游。重组融合蛋白在细菌中表达，然后通过固定化金属亲和层析纯化。 ELISA和Western印迹结果表明，融合蛋白不仅保留了VEE抗原结合和特异性，与其母体天然单克隆抗体相似，而且具有链霉抗生物素蛋白结合活性。这一发现消除了对抗体的化学生物素化和与抗体变性相关的风险的需要，并且提供用于快速和有效的VEE免疫测定的稳定且可重现的试剂。

6. 发明申请

US20050118569A1 Genetic engineering of streptavidin-binding peptide tagged single-chain variable fragment antibody to venezuelan equine encephalitis virus 失效
标题翻译：链霉亲和素结合肽标记的单链可变片段抗体对委内瑞拉马脑炎病毒的遗传工程
公开(公告)号：US20050118569A1
公开(公告)日：2005-06-02
申请号：US10784305
申请日：2004-02-24
申请人： R. Fulton , Leslie Nagata , Azhar Alvi , Weigang Hu
发明人： R. Fulton , Leslie Nagata , Azhar Alvi , Weigang Hu
IPC分类号： C07K14/47 , C07K16/08 , C07K16/10 , C07K19/00 , C12N15/10 , C12N15/66 , G01N33/53 , G01N33/569 , C12Q1/70 , C07H21/04 , C12N5/06 , C12P19/34 , C12Q1/68
CPC分类号： C07K16/1081 , C07K2317/622 , C07K2319/22
摘要： A recombinant gene encoding a single-chain variable fragment (scFv) antibody against Venezuelan equine encephalitis virus (VEE) being cloned into a prokaryotic T7 RNA polymerase-regulated expression vector was disclosed. A streptavidin-binding peptide (SBP) sequence fused to a 6His tag is then attached downstream to the scFv gene. The recombinant fusion protein is expressed in bacteria and then purified by immobilized metal affinity chromatography. ELISA and Western blotting results revealed that the fusion protein not only retained VEE antigen binding and specificity properties similar to those of its parent native monoclonal antibody, but also possessed streptavidin-binding activity. This discovery obviates the need for chemical biotinylation of antibodies and the risk associated with antibody denaturation and provides a stable and reproducible reagent for rapid and efficient immunoassay of VEE.
摘要翻译：公开了克隆到原核T7RNA聚合酶调节表达载体中的编码针对委内瑞兰马脑炎病毒（VEE）的单链可变片段（scFv）抗体的重组基因。然后将与6His标签融合的链霉抗生物素蛋白结合肽（SBP）序列连接到scFv基因的下游。重组融合蛋白在细菌中表达，然后通过固定化金属亲和层析纯化。 ELISA和Western印迹结果表明，融合蛋白不仅保留了VEE抗原结合和特异性，与其母体天然单克隆抗体相似，而且具有链霉抗生物素蛋白结合活性。这一发现消除了对抗体的化学生物素化和与抗体变性相关的风险的需要，并且提供用于快速和有效的VEE免疫测定的稳定且可重现的试剂。

7. 发明授权

US07317963B2 Method and system for generating trimlines for stamping processes 有权
标题翻译：用于生成冲压工艺的三角形的方法和系统
公开(公告)号：US07317963B2
公开(公告)日：2008-01-08
申请号：US11410809
申请日：2006-04-25
申请人： Weigang Hu , Gerald McLaughlin
发明人： Weigang Hu , Gerald McLaughlin
IPC分类号： G06F19/00
CPC分类号： G06F17/5004 , G06F2217/42
摘要： According to one embodiment of the present invention, an electronic method for generating a trimline for trimming sheet metal to be stamped to obtain a stamped part is disclosed. The method includes providing a CAD part file representing a part that can be stamped from a sheet metal material; receiving a selection of a part surface on the part to be stamped and a line on the surface; receiving an input of a sheet metal thickness for the sheet metal material; and generating a trimline based on the CAD part file, the line on the surface, and the sheet metal thickness.
摘要翻译：根据本发明的一个实施例，公开了一种电子方法，用于产生用于修剪待冲压的金属板的三角形以获得冲压部件。该方法包括提供表示可以从金属板材料冲压的部分的CAD零件文件; 接收要冲压的部件上的部分表面的选择和表面上的线; 接收用于所述金属板材料的金属板厚度的输入; 并基于CAD零件文件，表面上的线和钣金厚度生成三角形。

8. 发明授权

US07309566B2 Genetically biotinylated recombinant antibody in immunofiltration assay by light addressable potentiometric sensor for identification of Venezuelan equine encephalitis virus 失效
标题翻译：遗传生物素化重组抗体在免疫过滤测定中通过光寻址电位传感器鉴定委内瑞兰马脑炎病毒
公开(公告)号：US07309566B2
公开(公告)日：2007-12-18
申请号：US10807194
申请日：2004-03-24
申请人： R. Elaine Fulton , Leslie P. Nagata , Azhar Z. Alvi , Weigang Hu
发明人： R. Elaine Fulton , Leslie P. Nagata , Azhar Z. Alvi , Weigang Hu
IPC分类号： C07K16/10 , C07K16/08 , G01N33/543 , G01N33/569 , C12Q1/70
CPC分类号： C07K16/1081 , C07K2317/622 , G01N33/543 , G01N33/56983
摘要： A genetically biotinylated single chain fragment variable (scFv) antibody against Venezuelan equine encephalitis virus (VEE) being applied in a system consisting of an immunofiltration-enzyme assay (IFA) with a light addressable potentiometric sensor (LAPS) for the rapid identification of VEE is disclosed. The IFA entails formation of an immunocomplex sandwich consisting of VEE, biotinylated antibody, fluoresceinated antibody and streptavidin, capturing the sandwich by filtration on biotinylated membrane, and detecting the sandwich by anti-fluorescein urease conjugate. The concentration ratio of biotinylated to fluoresceinated antibodies is investigated and optimized. The IFA/LAPS assay sensitivity was approximately equal to that of a conventional enzyme-linked immunosorbant assay utilizing polystyrene plates and a chromogenic substrate, however, less time and effort were required for performance of the IFA/LAPS assay.
摘要翻译：在由用于快速鉴定VEE的可光寻址电位传感器（LAPS）组成的系统组成的系统中应用针对委内瑞兰马脑炎病毒（VEE）的遗传生物素化单链片段变量（scFv）抗体是披露 IFA需要形成由VEE，生物素化抗体，荧光抗体和链霉抗生物素蛋白组成的免疫复合物夹心，通过在生物素化膜上过滤捕获夹心物，并通过抗荧光素脲酶缀合物检测夹心。研究并优化生物素化至荧光抗体的浓度比。 IFA / LAPS测定灵敏度大约等于使用聚苯乙烯板和显色底物的常规酶联免疫吸附测定法，然而，对于IFA / LAPS测定的性能需要较少的时间和精力。

9. 发明申请

US20070250205A1 Method and system for generating trimlines for stamping processes 有权
标题翻译：用于生成冲压工艺的三角形的方法和系统
公开(公告)号：US20070250205A1
公开(公告)日：2007-10-25
申请号：US11410809
申请日：2006-04-25
申请人： Weigang Hu , Gerald McLaughlin
发明人： Weigang Hu , Gerald McLaughlin
IPC分类号： G06F19/00
CPC分类号： G06F17/5004 , G06F2217/42
摘要： According to one embodiment of the present invention, an electronic method for generating a trimline for trimming sheet metal to be stamped to obtain a stamped part is disclosed. The method includes providing a CAD part file representing a part that can be stamped from a sheet metal material; receiving a selection of a part surface on the part to be stamped and a line on the surface; receiving an input of a sheet metal thickness for the sheet metal material; and generating a trimline based on the CAD part file, the line on the surface, and the sheet metal thickness.
摘要翻译：根据本发明的一个实施例，公开了一种电子方法，用于产生用于修剪待冲压的金属板的三角形以获得冲压部件。该方法包括提供表示可以从金属板材料冲压的部分的CAD零件文件; 接收要冲压的部件上的部分表面的选择和表面上的线; 接收用于所述金属板材料的金属板厚度的输入; 并基于CAD零件文件，表面上的线和钣金厚度生成三角形。

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