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1. 发明授权

US07498480B2 Cytoplasmic gene inhibition or gene expression in transfected plants by a tobraviral vector 失效
标题翻译：通过载体对转染植物进行细胞质基因抑制或基因表达
公开(公告)号：US07498480B2
公开(公告)日：2009-03-03
申请号：US10634221
申请日：2003-08-04
申请人： Peter D. Roberts , Monto H. Kumagai , Andrew A. Vaewhongs
发明人： Peter D. Roberts , Monto H. Kumagai , Andrew A. Vaewhongs
IPC分类号： C12N15/83 , C12N5/04 , C12N15/82
CPC分类号： A01H1/04 , A01H5/12 , C12N15/1034 , C12N15/8261 , C12Q1/68 , Y02A40/146
摘要： This invention is directed to a monopartite RNA viral vector comprising modified tobravirus RNA-1 comprising an inserted foreign RNA sequence. This invention is also directed to a bipartite RNA viral vector derived from a tobravirus, wherein the vector comprises one or more foreign RNA sequences. The invention is directed to a method of silencing one or more endogenous plant host genes and a method of simultaneously silencing a plant host gene and expressing a foreign gene in a plant host. Such methods comprise infecting a plant host with a bipartite vector comprising modified tobravirus RNA-1 and RNA-2. The invention is further directed to a method of compiling a plant functional gene profile, a method of changing the phenotype or biochemistry of a plant host, and a method of determining the presence of a trait in a plant host, using a monopartite or bipartite viral vector derived from a tobravirus.
摘要翻译：本发明涉及包含经插入的外源RNA序列的修饰的载体RNA-1的单分子RNA病毒载体。本发明还涉及衍生自托巴威病毒的二分之一RNA病毒载体，其中所述载体包含一个或多个外源RNA序列。本发明涉及沉默一种或多种内源性植物宿主基因的方法和同时使植物宿主基因沉默并在植物宿主中表达外源基因的方法。这样的方法包括用包含修饰的tobravirus RNA-1和RNA-2的二重载体感染植物宿主。本发明进一步涉及一种编码植物功能基因谱的方法，改变植物宿主的表型或生物化学的方法，以及使用单一或二分之一病毒测定植物宿主中性状的存在的方法衍生自Tobravirus的载体。

2. 发明授权

US07582423B2 Population of polynucleotide sequence variants 失效
标题翻译：多核苷酸序列变体种群
公开(公告)号：US07582423B2
公开(公告)日：2009-09-01
申请号：US10280913
申请日：2002-10-25
申请人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice
发明人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12N15/102 , C12N15/1027
摘要： We describe here an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences, by making a heteroduplex polynucleotide from two non-identical polynucleotides; introducing a nick in one strand at or near a base pair mismatch site; removing mismatched base(s) from the mismatch site where the nick occurred; and using the opposite strand as template to replace the removed base(s) with bases that complement base(s) in the first strand. By this method, information is transferred from one strand to the other at sites of mismatch.
摘要翻译：我们在这里描述了通过从两个不相同的多核苷酸制备异源双链多核苷酸来重新分配不相同的多核苷酸序列之间的序列变异的体外方法; 在碱基对失配位点处或附近在一条链中引入切口; 从错配发生的不匹配位置去除错配的碱基; 并使用相反的链作为模板以用第一链中的碱基补充的碱替换去除的碱基。通过这种方法，信息在不匹配的位置从一条链路转移到另一条链路。

3. 发明申请

US20210108221A1 ENDOSTATIN FRAGMENTS AND VARIANTS FOR USE IN TREATING FIBROSIS 有权
公开(公告)号：US20210108221A1
公开(公告)日：2021-04-15
申请号：US17092539
申请日：2020-11-09
申请人： iBio, Inc. , Novici Biotech LLC , MUSC Foundation for Research Development
发明人： Carol Feghali-Bostwick , Terence E. Ryan , Hal S. Padgett , Matthew McGee
IPC分类号： C12N15/82
摘要： Materials and methods for using polypeptides containing fragments and variants of endostatin to treat fibrosis are described herein.

4. 发明授权

US08148145B2 Virus coat protein variants with self-subtracting properties 失效
标题翻译：具有自减性质的病毒外壳蛋白变体
公开(公告)号：US08148145B2
公开(公告)日：2012-04-03
申请号：US12431622
申请日：2009-04-28
申请人： Hal S. Padgett , Fakhrieh S. Vojdani
发明人： Hal S. Padgett , Fakhrieh S. Vojdani
IPC分类号： C12N15/00 , C07H21/02 , A61K48/00
CPC分类号： C07K14/005 , C12N2770/00022
摘要： Herein is described a modified viral vector comprising: a coat protein modified, for example by the addition of a cysteine residue, such that the modified viral vector yields less soluble virus relative to that from an unmodified viral vector upon extraction of plant material infected with the modified viral vector, thereby facilitating purification of a recombinant protein expressed from the modified viral vector. Also described is a method of reducing viral coat protein impurities during purification of a recombinant protein, a method of biocontainment for a recombinant viral vector, and a method of generating virus inoculum for the modified viral vector.
摘要翻译：本文描述了修饰的病毒载体，其包含：例如通过加入半胱氨酸残基修饰的外壳蛋白，使得当提取感染了所述病毒载体的植物材料时，所述修饰的病毒载体相对于未修饰的病毒载体产生的可溶性病毒修饰的病毒载体，从而促进从经修饰的病毒载体表达的重组蛋白质的纯化。还描述了在重组蛋白质的纯化期间减少病毒外壳蛋白杂质的方法，重组病毒载体的生物达标方法，以及为修饰的病毒载体产生病毒接种物的方法。

5. 发明授权

US07838219B2 Method of increasing complementarity in a heteroduplex 有权
标题翻译：在异源双链中增加互补性的方法
公开(公告)号：US07838219B2
公开(公告)日：2010-11-23
申请号：US10637758
申请日：2003-08-08
申请人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice , Andrew A. Vaewhongs
发明人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice , Andrew A. Vaewhongs
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12N15/1027 , C12N15/102
摘要： We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.
摘要翻译：我们在这里描述了增加异源双链多核苷酸序列中的互补性的体外方法。该方法使用相反链的退火以形成具有错配的多核苷酸双链体。将异源双链多核苷酸与有效量的具有链切割活性，3'至5'核酸外切酶活性和聚合酶活性的酶组合，并且允许足够的时间在异源双链体内增加互补百分比。并非所有的异源双链多核苷酸都必然具有解决互补性的所有错配。任选地连接得到的多核苷酸。产生几种变异多核苷酸。在相对链中的任一个在另一条链中模板记录的位点，异源双链多核苷酸序列的所得百分比互补性增加。在退火异源链之前，母体多核苷酸不需要切割成片段。因此，不需要重新组装。

6. 发明授权

US07833759B2 Method of increasing complementarity in a heteroduplex 有权
公开(公告)号：US07833759B2
公开(公告)日：2010-11-16
申请号：US11768157
申请日：2007-06-25
申请人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice
发明人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice
IPC分类号： C12P19/34 , C12Q1/68 , C07H21/02 , C07H21/04 , C07H21/00
CPC分类号： C12N15/102 , C12N15/1027
摘要： We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.

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