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1. 发明申请

US20190194687A1 EFFICIENT NON-MEIOTIC ALLELE INTROGRESSION IN LIVESTOCK 审中-公开
公开(公告)号：US20190194687A1
公开(公告)日：2019-06-27
申请号：US16294619
申请日：2019-03-06
申请人： Scott C. Fahrenkrug , Daniel F. Carlson , Tad S. Sonstegard
发明人： Scott C. Fahrenkrug , Daniel F. Carlson , Tad S. Sonstegard
IPC分类号： C12N15/85 , C12N15/90 , A01K67/027 , C12N15/877 , C07K14/47 , C07K14/715
CPC分类号： C12N15/85 , A01K67/0275 , C07K14/47 , C07K14/715 , C12N15/8509 , C12N15/8771 , C12N15/8778 , C12N15/907 , C12N2750/14143 , C12N2800/30 , C12N2800/90
摘要： Methods, uses, and animals for introgression of alleles between animals, including SNPs. One embodiment involves introducing a targeted targeting endonuclease system and a HDR template into a cell with a mismatch in the binding of the targeting endonuclease and the targeted site.

2. 发明申请

US20180051298A1 EFFICIENT NON-MEIOTIC ALLELE INTROGRESSION 审中-公开
公开(公告)号：US20180051298A1
公开(公告)日：2018-02-22
申请号：US15802272
申请日：2017-11-02
申请人： Recombinetics, Inc.
发明人： Scott C. Fahrenkrug , Daniel F. Carlson
IPC分类号： C12N15/85 , C12N15/877 , C07K14/47 , C07K14/715 , C12N15/90 , A01K67/027
CPC分类号： C12N15/85 , A01K67/0275 , C07K14/47 , C07K14/715 , C12N15/8509 , C12N15/8771 , C12N15/8778 , C12N15/907 , C12N2750/14143 , C12N2800/30 , C12N2800/90
摘要： Methods, uses, and animals for introgression of alleles between animals, including SNPs. One embodiment involves introducing a targeted targeting endonuclease system and a HDR template into a cell with a mismatch in the binding of the targeting endonuclease and the targeted site.

3. 发明申请

US20120246746A1 Method for Generating Immune-Compatible Cells and Tissues Using Nuclear Transfer Techniques 审中-公开
标题翻译：使用核转移技术产生免疫兼容细胞和组织的方法
公开(公告)号：US20120246746A1
公开(公告)日：2012-09-27
申请号：US13108666
申请日：2011-05-16
申请人： Robert Lanza , Michael West
发明人： Robert Lanza , Michael West
IPC分类号： A01K67/00 , A61K48/00 , A61K49/00
CPC分类号： C12N15/873 , A01K67/0271 , A01K2217/05 , A01K2227/101 , A01K2227/105 , A01K2267/025 , A61K49/0008 , C12N15/8771 , C12N2517/04
摘要： This invention relates to methods for making immune compatible tissues and cells for the purpose of transplantation and tissue engineering, using the techniques of nuclear transfer and cloning. Also encompassed are methods for determining the effect on immune compatibility of expressed transgenes and other genetic manipulations of the engineered cells and tissues.
摘要翻译：本发明涉及使用核转移和克隆技术制备用于移植和组织工程的免疫相容性组织和细胞的方法。还包括确定表达的转基因对工程细胞和组织的免疫相容性和其他遗传操作的影响的方法。

4. 发明授权

US07524677B2 Mammalian cultured inner cell mass cell culture using a g1 cell as nuclear donor 失效
标题翻译：使用g1细胞作为核供体的哺乳动物培养的内细胞大量细胞培养
公开(公告)号：US07524677B2
公开(公告)日：2009-04-28
申请号：US11265114
申请日：2005-11-03
申请人： Keith Henry Stockman Campbell , Ian Wilmut
发明人： Keith Henry Stockman Campbell , Ian Wilmut
IPC分类号： C12N15/00
CPC分类号： C12N15/8773 , A61K35/54 , C12N15/873 , C12N15/8771
摘要： A method of reconstituting an animal embryo involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at the time of transfer, so that the donor nucleus is kept exposed to the recipient cytoplasm for a period of time. The diploid nucleus can be donated by a cell in either the G0 or G1 phase of the cell cycle at the time of transfer. Subsequently, the reconstituted embryo is activated. Correct ploidy is maintained during activation, for example, by incubating the reconstituted embryo in the presence of a microtubule inhibitor such as nocodazole. The reconstituted embryo may then give rise to one or more live animal births. The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit.
摘要翻译：一种重建动物胚胎的方法涉及将二倍体核转移到在第二次减数分裂期的中期被逮捕的卵母细胞。卵母细胞在转移时不被激活，使得供者核细胞保持暴露于受体细胞质一段时间。二倍体核可以在转移时由细胞G0或G1期的细胞捐献。随后，重建的胚胎被激活。在活化期间维持正确的倍性，例如通过在微管抑制剂例如诺考达唑的存在下孵育重构的胚胎。然后，重建的胚胎可能会产生一种或多种活的动物分娩。本发明可用于生产转基因动物以及具有高遗传优点的非转基因植物。

5. 发明授权

US07514258B2 Mammalian cultured inner cell mass cell culture using a quiescent cell as nuclear 失效
标题翻译：使用静止细胞作为核供体的哺乳动物培养的内细胞大量细胞培养
公开(公告)号：US07514258B2
公开(公告)日：2009-04-07
申请号：US11265113
申请日：2005-11-03
申请人： Keith Henry Stockman Campbell , Ian Wilmut
发明人： Keith Henry Stockman Campbell , Ian Wilmut
IPC分类号： C12N15/00
CPC分类号： C12N15/8771 , A61K35/54 , C12N15/8773
摘要： A method of reconstituting an animal embryo involves transferring the nucleus from a quiescent donor cell into a suitable recipient cell. The donor cell is quiescent, in that it is caused to exit from the growth and division cycle at G1 and to arrest in the G0 state. Nuclear transfer may take place by cell fusion. The reconstituted embryo may then give rise to one or more animals. The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit.
摘要翻译：重建动物胚胎的方法涉及将细胞核从静止供体细胞转移到合适的受体细胞中。供体细胞是静止的，因为它被导致在G1期的生长和分裂周期退出并且在G0状态下停止。核转移可能通过细胞融合发生。然后，重构的胚胎可以产生一种或多种动物。本发明可用于生产转基因动物以及具有高遗传优点的非转基因植物。

6. 发明申请

US20070143868A1 Sperm Cell Processing and Preservation Systems 审中-公开
标题翻译：精子细胞处理和保存系统
公开(公告)号：US20070143868A1
公开(公告)日：2007-06-21
申请号：US11668148
申请日：2007-01-29
申请人： William Maxwell , Fiona Hollinshead , Justine O'Brien , Gareth Evans
发明人： William Maxwell , Fiona Hollinshead , Justine O'Brien , Gareth Evans
IPC分类号： A01K67/027 , A01N1/02
CPC分类号： C12N5/0604 , A01K2227/101 , A01K2227/103 , A61B17/43 , C12N5/061 , C12N15/873 , C12N15/8771 , C12N15/8773 , C12N2517/10
摘要： Semen and sperm cell processing and preservation systems, and methods of producing a mammal and methods of producing mammalian embryos are disclosed. The present invention is directed to sperm cell preservation, fertilization, and insemination, maintaining or enhancing sperm quality and addressing one or more sperm cell characteristics, such as viability, motility, functionality, fertilization rates, and pregnancy rates. Further, sperm cell characteristics may be addressed within the context of various collection, handling, separation, storage, transportation, usage, fertilization, or insemination techniques.
摘要翻译：公开了精液和精子细胞的处理和保存系统，以及哺乳动物的产生方法和哺乳动物胚胎的制备方法。本发明涉及精子细胞保存，受精和授精，维持或提高精子质量并解决一种或多种精子细胞特征，如活力，活力，功能，受精率和妊娠率。此外，可以在各种收集，处理，分离，储存，运输，使用，受精或授精技术的背景下解决精子细胞特征。

7. 发明授权

US07169548B2 Sperm cell processing and preservation systems 有权
标题翻译：精子细胞处理和保存系统
公开(公告)号：US07169548B2
公开(公告)日：2007-01-30
申请号：US10340881
申请日：2003-01-09
申请人： William Maxwell Chisholm Maxwell , Fiona Kate Hollinshead , Justine Kellie O'Brien , Gareth Evans
发明人： William Maxwell Chisholm Maxwell , Fiona Kate Hollinshead , Justine Kellie O'Brien , Gareth Evans
IPC分类号： A01N1/02
CPC分类号： C12N5/0604 , A01K2227/101 , A01K2227/103 , A61B17/43 , C12N5/061 , C12N15/873 , C12N15/8771 , C12N15/8773 , C12N2517/10
摘要： Methods and apparatus are disclosed for processing sperm cells to accomplish preservation for future use while minimizing the adverse effects of such preservation. Sperm cells may be collected from a male animal and subjected to a first preservation step, including potentially a first cryopreservation step. Preserved sperm may then be revived, including potentially by thawing, and treated by any of various processing steps to mitigate the adverse effects of preservation. Treated sperm may then be subjected to a second preservation step, including potentially a second cryopreservation step, perhaps enabling a delayed use of the sperm at a future time.
摘要翻译：公开了用于处理精子细胞以完成保存以备将来使用的方法和装置，同时最小化这种保存的不利影响。可以从雄性动物收集精子细胞并进行第一保存步骤，包括潜在的第一次冷冻保存步骤。然后可以恢复保存的精子，包括潜在的解冻，并通过任何各种加工步骤进行处理以减轻保存的不利影响。然后处理的精子可以进行第二保留步骤，包括潜在的第二次冷冻保存步骤，或许能够在将来的时间延迟使用精子。

8. 发明申请

US20050172347A1 Unactivated oocytes as cytoplast recipients for nuclear transfer 有权
标题翻译：未激活的卵母细胞作为核转移的细胞质受体
公开(公告)号：US20050172347A1
公开(公告)日：2005-08-04
申请号：US11068903
申请日：2005-03-02
申请人： Keith Campbell , Ian Wilmut
发明人： Keith Campbell , Ian Wilmut
IPC分类号： A01K67/02 , A61K35/54 , C12N5/10 , C12N15/02 , C12N15/873 , C12N15/877 , A01K67/027
CPC分类号： C12N15/8773 , A61K35/54 , C12N15/873 , C12N15/8771
摘要： A method of reconstituting an animal embryo involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at the time of transfer, so that the donor nucleus is kept exposed to the recipient cytoplasm for a period of time. The diploid nucleus can be donated by a cell in either the G0 or G1 phase of the cell cycle at the time of transfer. Subsequently, the reconstituted embryo is activated. Correct ploidy is maintained during activation, for example, by incubating the reconstituted embryo in the presence of a microtubule inhibitor such as nocodazole. The reconstituted embryo may then give rise to one or more live animal births. The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit.
摘要翻译：重建动物胚胎的方法涉及将二倍体核转移到在第二次减数分裂期的中期被逮捕的卵母细胞。卵母细胞在转移时不被激活，使得供者核细胞保持暴露于受体细胞质一段时间。二倍体核可以在转移时由细胞G0或G1期的细胞捐献。随后，重建的胚胎被激活。在活化期间维持正确的倍性，例如通过在微管抑制剂例如诺考达唑的存在下孵育重构的胚胎。然后，重建的胚胎可能会产生一种或多种活的动物分娩。本发明可用于生产转基因动物以及具有高遗传优点的非转基因植物。

9. 发明申请

US20040194159A1 Cloning pigs using donor nuclei from differentiated cells 审中-公开
标题翻译：使用来自分化细胞的供体核克隆猪
公开(公告)号：US20040194159A1
公开(公告)日：2004-09-30
申请号：US10817573
申请日：2004-04-02
申请人： University of Massachusetts
发明人： Steven L. Stice , James M. Robl , Jose Cibelli , Paul Golueke
IPC分类号： A01K067/027
CPC分类号： C12N15/8778 , A01K67/027 , A01K67/0273 , A01K67/0275 , A01K2217/05 , A01K2227/101 , A61K35/12 , A61K38/00 , C12N5/0603 , C12N15/8771 , C12N2510/00 , C12N2517/02 , C12N2517/04
摘要： An improved method of nuclear transfer involving the transplantation of donor differentiated pig cell nuclei into enucleated pig oocytes is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring. Production of genetically engineered or transgenic pig embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.
摘要翻译：提供了涉及将供体分化的猪细胞核移植到去核猪卵母细胞中的改进的核转移方法。所产生的核转移单位可用于通过胎儿和后代的产生来增殖基因型和转基因基因型。通过本发明的方法促进转基因植物或转基因猪胚胎，胎儿和后代的生产，因为供体核的分化细胞源可被遗传修饰和克隆繁殖。

10. 发明申请

US20040040050A1 Production of agricultural animals from embryonic stem (es) cells 审中-公开
标题翻译：从胚胎干细胞生产农业动物
公开(公告)号：US20040040050A1
公开(公告)日：2004-02-26
申请号：US10276001
申请日：2003-06-06
发明人： Michael D West
IPC分类号： A01K067/027 , G06F017/60
CPC分类号： C12N15/873 , A01K67/0273 , A01K2217/05 , A01K2227/101 , C12N15/8771
摘要： This invention describes a novel method of agricultural animal production that provides animals with optimum growth characteristics in a reliable and cost-effective manner. The invention utilizes a novel combination of Nuclear Transfer (NT) and Embryonic Stem (ES) cell technologies that improves the efficiency of delivering optimized animals and facilitates the introduction of genetic modifications into farm animals.
摘要翻译：本发明描述了一种以可靠和具有成本效益的方式为动物提供最佳生长特性的新型农业动物生产方法。本发明利用核转移（NT）和胚胎干细胞（ES）细胞技术的新型组合，其提高了递送优化动物的效率，并有助于将遗传修饰引入农场动物。

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