专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20180249688A1 Multi-Transgenic Pig for Xenotransplantation 审中-公开
公开(公告)号：US20180249688A1
公开(公告)日：2018-09-06
申请号：US15758895
申请日：2016-09-09
申请人： REVIVICOR, INC
发明人： David L. AYARES , Carol PHELPS
IPC分类号： A01K67/027 , C12N15/85 , C12N15/877
CPC分类号： A01K67/0278 , A01K67/0275 , A01K2217/052 , A01K2217/072 , A01K2217/15 , A01K2217/206 , A01K2227/108 , A01K2267/025 , A01N1/0226 , C07K2319/50 , C12N15/8509 , C12N15/8778 , C12N15/907 , C12N2800/30 , C12N2840/20
摘要： The present invention is directed to transgenic animals (e.g., transgenic porcine animals) comprising multiple genetic modifications that advantageously render these animals suitable donors for xenotransplanation. The present invention extends to organs, organ fragments, tissues and cells derived from these animals and their therapeutic use. The present invention further extends to methods of making such animals. In certain embodiments, the transgenic animals (e.g., transgenic porcine animals) lack expression of alpha gal and incorporate and express at least four transgenes under the control of at least two promoters.

2. 发明申请

US20150156996A1 LIVESTOCK WITH GENETICALLY MODIFIED PROLACTIN RECEPTOR 审中-公开
标题翻译： LIVESTOCK与基因改造的PROLACTIN受体
公开(公告)号：US20150156996A1
公开(公告)日：2015-06-11
申请号：US14625797
申请日：2015-02-19
申请人： Recombinetics, Inc.
发明人： Scott C. Fahrenkrug , Daniel F. Carlson
IPC分类号： A01K67/027 , C07K14/47 , C12N15/85 , C07K14/715
CPC分类号： C12N15/85 , A01K67/0275 , C07K14/47 , C07K14/715 , C12N15/8509 , C12N15/8771 , C12N15/8778 , C12N15/907 , C12N2750/14143 , C12N2800/30 , C12N2800/90
摘要： Methods, uses, and animals for introgression of alleles between animals, including SNPs. One embodiment involves introducing a targeted targeting endonuclease system and a HDR template into a cell with a mismatch in the binding of the targeting endonuclease and the targeted site.
摘要翻译：方法，用途和动物对动物之间的等位基因进行渗入，包括SNP。一个实施方案涉及将靶向靶向内切核酸酶系统和HDR模板引入具有靶向内切核酸酶与靶位点的结合不匹配的细胞中。

3. 发明申请

US20130150465A1 PIG MODEL FOR BREAST CANCER, MITOCHONDRIA RELATED PROTEIN FOLDING DISORDERS AND/OR EPIDERMOLYSIS BULLOSA SIMPLEX 审中-公开
标题翻译： PIG型乳腺癌，MITOCHONDRIA相关蛋白折叠病和/或EPIDERMOLYSIS BULLOSA SIMPLEX
公开(公告)号：US20130150465A1
公开(公告)日：2013-06-13
申请号：US13592045
申请日：2012-08-22
申请人： Peter Michael Kragh , Lars Axel Bolund , Charlotte Brandt Sørensen , Thomas Juhl Corydon , Niels Gregersen , Peter Bross
发明人： Peter Michael Kragh , Lars Axel Bolund , Charlotte Brandt Sørensen , Thomas Juhl Corydon , Niels Gregersen , Peter Bross
IPC分类号： A01K67/027
CPC分类号： A01K67/0275 , A01K67/0271 , A01K2217/052 , A01K2227/108 , A01K2267/0331 , C12N15/8509 , C12N15/8778 , C12N2800/30 , C12N2800/90
摘要： The present invention relates to a genetically modified pig as a model for studying breast cancer, mitochondria related protein folding disorders and/or epidermolysis bullosa simplex. The modified pig model displays one or more phenotypes associated with any of said disorders. Disclosed is also a modified pig comprising a modified endogeneous BRCA1 and/or BRCA2 gene, and/or a modified ornithine transcarbamylase gene, and/or a modified Keratin 14 gene and/or a transcriptional or translational product or part thereof. The invention further relates to methods for producing the modified pig; and methods for evaluating the effect of a therapeutical treatment of breast cancer, mitochondria related protein folding disorders and/or epidermolysis bullosa simplex; methods for screening the efficacy of a pharmaceutical composition; and a method for treatment of a human being suffering from breast cancer, mitochondria related protein folding disorders and/or epidermolysis bullosa simplex are disclosed.
摘要翻译：本发明涉及一种转基因猪作为研究乳腺癌，线粒体相关蛋白折叠病和/或大疱性表皮松解症的模型。修改的猪模型显示与任何所述疾病相关的一种或多种表型。还公开了包含修饰的内源性BRCA1和/或BRCA2基因和/或修饰的鸟氨酸转氨酶基因和/或修饰的角蛋白14基因和/或转录或翻译产物或其部分的修饰的猪。本发明还涉及生产改性猪的方法; 以及用于评估乳腺癌，线粒体相关蛋白折叠病和/或大疱性表皮松解症的治疗效果的评价方法。用于筛选药物组合物的功效的方法; 公开了一种治疗患有乳腺癌，线粒体相关蛋白折叠病和/或大疱表皮松解症的人的方法。

4. 发明申请

US20100115641A1 GFP-TRANSFECTED CLON PIG, GT KNOCK-OUT CLON PIG AND METHODS FOR PRODUCTIONS THEREOF 审中-公开
标题翻译：绿色荧光蛋白转移克隆PIG，GT KNOCK-OUT CON PIG及其生产方法
公开(公告)号：US20100115641A1
公开(公告)日：2010-05-06
申请号：US12579032
申请日：2009-10-14
申请人： So H. Lee , Woo S. Hwang , Byeong C. Lee , Sung K. Kang , Jek Y. Han , Jeong M. Lim , Chang K. Lee , Eun S. Lee , Eui B. Jeung , Jong K. Cho , Dae Y. Kim , Sang H. Hyun , Gab S. Lee , Hye S. Kim , Sung C. Lee , Su C. Yeom
发明人： So H. Lee , Woo S. Hwang , Byeong C. Lee , Sung K. Kang , Jek Y. Han , Jeong M. Lim , Chang K. Lee , Eun S. Lee , Eui B. Jeung , Jong K. Cho , Dae Y. Kim , Sang H. Hyun , Gab S. Lee , Hye S. Kim , Sung C. Lee , Su C. Yeom
IPC分类号： A01K67/027 , C12N15/11 , C12N15/85
CPC分类号： C12N15/8778
摘要： Disclosed are a cloned pig expressing green fluorescent protein (GFP) and a cloned pig having a 1,3-galactosyltransferase (GT) gene knocked out. Also, the present invention discloses methods of producing such cloned pigs, comprising the steps of establishing a somatic cell line; preparing a GFP-transfected or GT gene knock-out nuclear donor cell; producing a transgenic nuclear transfer embryo using the nuclear donor cell and a recipient oocyte; and transplanting the transgenic nuclear transfer embryo into a surrogate mother pig. The cloned pig expressing GFP of the present invention is useful for large-scale production of an animal disease model, and the GT gene knock-out cloned pig can be used as a organ donor allowing xenotransplantation in humans without hyperacute immune rejection.
摘要翻译：公开了表达绿色荧光蛋白（GFP）的克隆猪和敲除了1,3-半乳糖基转移酶（GT）基因的克隆猪。此外，本发明公开了生产这种克隆猪的方法，包括建立体细胞系的步骤; 制备GFP转染或GT基因敲除核供体细胞; 使用核供体细胞和受体卵母细胞产生转基因核转移胚胎; 并将转基因核转移胚胎移植到替代母猪中。表达本发明的GFP的克隆猪可用于大规模生产动物疾病模型，并且GT基因敲除克隆的猪可用作器官供体，其允许在人中进行异种移植而没有超急性免疫排斥。

5. 发明授权

US07601884B2 Method of producing cloned animals by demecolcine treatment 有权
标题翻译：通过脱敏治疗产生克隆动物的方法
公开(公告)号：US07601884B2
公开(公告)日：2009-10-13
申请号：US11968745
申请日：2008-01-03
申请人： Eunsong Lee
发明人： Eunsong Lee
IPC分类号： C12N15/00 , C12N5/00 , C12N5/02 , A01K67/027
CPC分类号： C12N15/8778 , A01K67/0273 , A01K2227/108
摘要： A cloned animal is produced by demecolcine treatment characterized by culturing the nuclear transfer embryo in vitro and transferring the embryo in vivo. This significantly improves in vitro development of somatic cell nuclear transfer embryos and maintenance of pregnancy from transfer of transfer somatic cell nuclear transfer embryos in a surrogate mother up to delivery. A nuclear transfer embryo of a non-primate mammal made by enucleating a recipient oocyte; injecting a nuclear donor cell into the enucleated oocyte; fusing together the injected enucleated oocyte with the injected nuclear donor cell to form a fused oocyte; activating the fused oocyte; and treating the activated oocyte with demecolcine to form the nuclear transfer embryo.
摘要翻译：克隆动物通过脱敏处理产生，其特征在于体外培养核转移胚并在体内转移胚胎。这显着改善了体细胞核转移胚胎的体外发育和维持妊娠转移体细胞核转移胚胎在代孕母亲直到分娩。通过摘除受体卵母细胞制成的非灵长类哺乳动物的核转移胚胎; 将核供体细胞注射到去核卵母细胞中; 将注射的去核卵母细胞与注射的核供体细胞融合以形成融合的卵母细胞; 激活融合的卵母细胞; 用灭丹子处理活化的卵母细胞以形成核转移胚。

6. 发明授权

US07547816B2 α(1,3)-galactosyltransferase knockout swine, tissues and organs 有权
标题翻译： α（1,3） - 半乳糖转移酶敲除猪，组织和器官
公开(公告)号：US07547816B2
公开(公告)日：2009-06-16
申请号：US10499407
申请日：2002-12-23
申请人： Billy N. Day , Robert J. Hawley , Randall S. Prather
发明人： Billy N. Day , Robert J. Hawley , Randall S. Prather
IPC分类号： A01K67/027 , C12N15/00 , A01N1/00
CPC分类号： C12N15/8509 , A01K67/0271 , A01K67/0276 , A01K2217/075 , A01K2227/108 , A01K2267/02 , A01K2267/025 , A01K2267/03 , C12N9/1051 , C12N15/8778 , C12N2517/02 , C12N2800/60 , C12N2840/203 , C12N2840/85
摘要： The invention relates to the genetic manipulation of non-human animals. More particularly, the invention relates to genetic manipulation of non-human animals to be used for xenotransplantation. The invention provides viable gene knockout swine including swine in which the α(1,3)-galactosyltransferase gene has been disrupted, methods for making such swine, and methods of using the tissues and organs of such swine for xenotransplantation.
摘要翻译：本发明涉及非人类动物的遗传操作。更具体地说，本发明涉及用于异种移植的非人动物的遗传操作。本发明提供了可行的基因敲除猪，包括其中α（1,3） - 半乳糖转移酶基因被破坏的猪，以及制备这种猪的方法，以及使用这种猪的组织和器官进行异种移植的方法。

7. 发明授权

US07291764B1 Cloning pigs using non-quiescent differentiated donor cells or nuclei 有权
标题翻译：使用非静止分化供体细胞或细胞核克隆猪
公开(公告)号：US07291764B1
公开(公告)日：2007-11-06
申请号：US09394902
申请日：1999-09-13
申请人： Steven L. Stice , Jose Cibelli , James Robl , Paul Golueke
发明人： Steven L. Stice , Jose Cibelli , James Robl , Paul Golueke
IPC分类号： C12N15/00 , C12N5/00 , A01K67/00
CPC分类号： C12N15/8778
摘要： An improved method of nuclear transfer involving the transplantation of donor differentiated pig cell nuclei into enucleated pig oocytes is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring. Production of genetically engineered or transgenic pig embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.
摘要翻译：提供了涉及将供体分化的猪细胞核移植到去核猪卵母细胞中的改进的核转移方法。所产生的核转移单位可用于通过胎儿和后代的产生来增殖基因型和转基因基因型。通过本发明的方法促进转基因植物或转基因猪胚胎，胎儿和后代的生产，因为供体核的分化细胞源可被遗传修饰和克隆繁殖。

8. 发明申请

US20070107074A1 Porcine Nuclear Transfer 审中-公开
标题翻译：猪核转运
公开(公告)号：US20070107074A1
公开(公告)日：2007-05-10
申请号：US11562852
申请日：2006-11-22
申请人： Paul Verma , Mark Nottle
发明人： Paul Verma , Mark Nottle
IPC分类号： A01K67/027 , C12N15/87
CPC分类号： C12N15/8778
摘要： A process for the production of nuclear transferred porcine embryonic cells which includes providing a porcine oocyte at the Metaphase II stage of development from which the nucleus is removed, transferring a porcine karyoplast at the G0 or G1 state into the oocyte to give a nuclear transferred porcine embryonic cell and optionally culturing the nuclear transferred cell in vitro to allow one or more cell divisions to give a plurality of nuclear transferred porcine embryonic cells is disclosed. Also disclosed is a process for the clonal generation or propagation of pigs which process includes providing a procine oocyte at the Metaphase II stage of development from which the nucleus is removed, transferring a porcine donor karyoplast at the G0 or G1 state into the oocyte to give a nuclear transferred porcine embryonic cell, and thereafter culturing the nuclear transferred cell in vitro to allow one or more cell divisions to give a plurality of nuclear transferred porcine embryonic cells, and thereafter transferring a plurality of porcine embryonic cells so produced into a pregnancy competent uterus of a female pig which at conclusion of the pregnancy term gives rise to one or more genetically identical off-spring.
摘要翻译：一种生产核转移猪胚胎细胞的方法，其包括在中期II期开发的细胞核中提供猪卵母细胞，将细胞核从G0或G1状态转移到卵母细胞中以产生转移的转基因猪胚胎细胞和任选地在体外培养核转移细胞以允许一个或多个细胞分裂产生多个核转移的猪胚胎细胞。还公开了一种用于克隆产生或繁殖猪的方法，该方法包括在中期II阶段提供原代卵母细胞，从其中除去细胞核，将在G0或G1状态的猪供体核型质粒转移到卵母细胞中，得到核转移的猪胚胎细胞，然后在体外培养核转移细胞，以允许一个或多个细胞分裂产生多个转移的转基因猪胚胎细胞，然后将如此产生的多个猪胚胎细胞转移到怀孕的子宫中在怀孕期结束时产生一种或多种基因相同的雌性猪。

9. 发明申请

US20060242722A1 Alpha(1,3)-galactosyltransferase null cells, methods of selecting and alpha(1,3)-galactosyl transferase null swine produced therefrom 有权
标题翻译： α（1,3） - 半乳糖基转移酶无效细胞，选择和产生α（1,3） - 半乳糖基转移酶无效猪的方法
公开(公告)号：US20060242722A1
公开(公告)日：2006-10-26
申请号：US10524381
申请日：2003-08-13
申请人： Robert Hawley
发明人： Robert Hawley
IPC分类号： A01K67/027 , C12N15/09 , C12N5/06
CPC分类号： A01K67/0276 , A01K2217/075 , A01K2227/108 , A01K2267/025 , C12N5/0611 , C12N9/1051 , C12N15/8509 , C12N15/8778 , C12N2517/02 , C12N2517/04
摘要： The invention relates to the genetic manipulation of non-human animals. More particularly, the invention relates to genetic manipulation of non-human animals to be used for xenotransplantation. The invention provides a method of selecting GGTA 1 null cells, a viable GGTA 1 null swine, methods for making such swine, and methods of using cells, tissues and organs of such swine for xenotransplantation.
摘要翻译：本发明涉及非人类动物的遗传操作。更具体地说，本发明涉及用于异种移植的非人动物的遗传操作。本发明提供了选择GGTA1无效细胞的方法，可行的GGTA 1无效猪，制备这种猪的方法，以及使用这种猪的细胞，组织和器官进行异种移植的方法。

10. 发明申请

US20060080746A1 Methods of embryo transfer 审中-公开
标题翻译：胚胎移植方法
公开(公告)号：US20060080746A1
公开(公告)日：2006-04-13
申请号：US11238297
申请日：2005-09-28
申请人： Scott Davis , Shawn Walker , Irina Polejaeva
发明人： Scott Davis , Shawn Walker , Irina Polejaeva
IPC分类号： A01K67/027
CPC分类号： A01K67/0273 , A01K2227/108 , A01K2267/02 , A01K2267/025 , C12N15/8778
摘要： Methods for the efficient production of cloned porcine fetuses/piglets following the production of cloned embryos, including culture of the embryos for extended periods prior to transfer of the embryos into the uterus of the recipient. Transfer can be accomplished surgically or through less-invasive laparoscopic or non-surgical transfer.
摘要翻译：在克隆胚胎生产之后有效生产克隆的猪胎儿/仔猪的方法，包括将胚胎转移到接受者的子宫之前长时间培养胚胎。转移可以通过手术或通过较少侵入性的腹腔镜或非手术转移来完成。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式