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1. 发明申请

US20160235045A1 Human Artificial Chromosome Vector 有权
标题翻译：人类人造染色体载体
公开(公告)号：US20160235045A1
公开(公告)日：2016-08-18
申请号：US15070842
申请日：2016-03-15
申请人： SAB, LLC
发明人： Yoshimi KUROIWA , Hiroaki MATSUSHITA , Akiko KANAI
IPC分类号： A01K67/027
CPC分类号： A01K67/0278 , A01K2207/15 , A01K2217/052 , A01K2217/072 , A01K2227/101 , A01K2267/01 , C07K16/00 , C07K16/1278 , C12N15/85 , C12N15/8509 , C12N2800/208
摘要： A human artificial chromosome vector comprising a gene encoding a human antibody heavy chain, a gene encoding a human antibody light chain, and a gene encoding an IgM heavy chain constant region derived from a nonhuman animal.
摘要翻译：包含编码人抗体重链的基因，编码人抗体轻链的基因和编码来自非人动物的IgM重链恒定区的基因的人类人造染色体载体。

2. 发明授权

US09139849B2 Rapid generation of long synthetic centromeric tandem repeats for mammalian artificial chromosome formation 有权
标题翻译：快速生成用于哺乳动物人造染色体形成的长期合成着丝粒串联重复序列
公开(公告)号：US09139849B2
公开(公告)日：2015-09-22
申请号：US11910973
申请日：2006-04-07
申请人： Vladimir L. Larionov , William C. Earnshaw , Reto Gassman , Stefanie Kandels-Lewis , Hiroshi Masumoto , Megumi Nakano , Vladimir Noskov , Natalay Y. Kouprina , Carl J. Barrett , Stefano Cardinale
发明人： Vladimir L. Larionov , William C. Earnshaw , Reto Gassman , Stefanie Kandels-Lewis , Hiroshi Masumoto , Megumi Nakano , Vladimir Noskov , Natalay Y. Kouprina , Carl J. Barrett , Stefano Cardinale
IPC分类号： C12P19/34 , C12Q1/68 , C12N15/85
CPC分类号： C12N15/85 , C12N2800/204 , C12N2800/206 , C12N2800/208 , C12N2830/003
摘要： Methods are described for construction of long synthetic arrays of DNA repeats, such as alphoid repeats or other repeat sequences. The methods include concatamerization of DNA into short repeats (for instance using rolling circle amplification or directional in vitro ligation), followed by assembling the short repeats into long arrays by homologous recombination during transformation into microbe cells. These methods can be described generally as Recombinational Amplification of Repeats (RAR). The long arrays are engineered centromere-like regions that allow one to construct mammalian artificial chromosomes with a predefined centromeric region structure. Artificial chromosomes, including human artificial chromosomes with a regulated centromere, and methods of their use are also provided.
摘要翻译：描述了构建DNA重复序列的长合成阵列的方法，例如同位素重复序列或其他重复序列。这些方法包括将DNA连接成短重复序列（例如使用滚环扩增或定向体外连接），然后通过在转化成微生物细胞期间的同源重组将短重复序列组装成长阵列。这些方法通常可以作为重复扩增重复（RAR）来描述。长阵列是工程化的着丝粒样区域，其允许用预定义的着丝粒区域结构构建哺乳动物人造染色体。还提供人造染色体，包括具有调节着丝粒的人造染色体及其使用方法。

3. 发明申请

US20150211020A1 COMPLEX CHROMOSOME ENGINEERING FOR PRODUCTION OF HUMAN ANTIBODIES IN TRANSGENIC ANIMALS 有权
标题翻译：复制染色体工程用于生产人类抗体在转基因动物
公开(公告)号：US20150211020A1
公开(公告)日：2015-07-30
申请号：US14416870
申请日：2013-08-05
申请人： SAB, LLC
发明人： Yoshimi Kuroiwa , Hiroaki Matsushita , Akiko Sano
IPC分类号： C12N15/85 , C07K16/46 , A01K67/027
CPC分类号： C12N15/8509 , A01K67/0278 , A01K2207/15 , A01K2217/072 , A01K2217/075 , A01K2227/101 , A01K2267/01 , C07K16/00 , C07K16/462 , C12N2015/8518 , C12N2800/208
摘要： The invention relates to large-scale production of human antibodies by transgenic animals with high production of fully human IgG up to >10 g/L in sera with human IgG1 subclass dominancy. This invention also supports a feasibility of complex chromosome engineering for complicated genetic studies in non-murine mammalian species.
摘要翻译：本发明涉及通过具有人IgG1亚类占优的血清中具有高达> 10g / L的完全人IgG高转基因动物的大规模生产。本发明还支持复杂染色体工程在非鼠类哺乳动物物种复杂遗传研究中的可行性。

4. 发明申请

US20120064631A1 METHOD FOR AMPLIFICATION AND HIGH-LEVEL EXPRESSION OF TARGET GENE IN MAMMALIAN CELL, AND KIT FOR ACHIEVING THE METHOD 审中-公开
标题翻译：用于在马马里亚细胞中靶向靶基因扩增和高水平表达的方法和用于实现方法的试剂盒
公开(公告)号：US20120064631A1
公开(公告)日：2012-03-15
申请号：US13260192
申请日：2010-03-24
申请人： Noriaki Shimizu
发明人： Noriaki Shimizu
IPC分类号： C12N15/85 , C12N5/10
CPC分类号： C12P21/02 , C12N15/79 , C12N15/85 , C12N2510/02 , C12N2800/208 , C12N2820/80 , C12N2830/46
摘要： The present invention provides novel means for amplifying a target gene outside a chromosome of the mammalian cell with a high probability in amplifying a target gene with use of IR/MAR plasmid, to further improve a high-level gene amplification system. The present invention is a method of amplifying a target gene outside a chromosome of a mammalian cell, and includes the step of transferring, concurrently to a mammalian cell, (i) a vector including (a) a mammalian replication initiation region functioning in a mammalian cell and (b) a nuclear matrix attachment region functioning in a mammalian cell, (ii) a target gene, and (iii) a polynucleotide including a telomere repetitive sequence.
摘要翻译：本发明提供了利用IR / MAR质粒扩增目标基因的高可能性来扩增哺乳动物细胞染色体外的靶基因以进一步改进高水平基因扩增系统的新手段。本发明是扩增哺乳动物细胞染色体外的靶基因的方法，包括同时向哺乳动物细胞转移的步骤，（i）载体，其包含（a）在哺乳动物中起作用的哺乳动物复制起始区细胞和（b）在哺乳动物细胞中起作用的核基质附着区，（ii）靶基因，和（iii）包含端粒重复序列的多核苷酸。

5. 发明申请

US20060246586A1 Chromosome-based platforms 审中-公开
标题翻译：基于染色体的平台
公开(公告)号：US20060246586A1
公开(公告)日：2006-11-02
申请号：US11480175
申请日：2006-06-29
申请人： Edward Perkins , Carl Perez , Michael Lindenbaum , Amy Greene , Josephine Leung , Elena Fleming , Sandra Stewart , Joan Shellard
发明人： Edward Perkins , Carl Perez , Michael Lindenbaum , Amy Greene , Josephine Leung , Elena Fleming , Sandra Stewart , Joan Shellard
IPC分类号： C12N5/08 , C12N15/09
CPC分类号： C07K14/005 , C12N9/22 , C12N15/63 , C12N15/85 , C12N15/90 , C12N15/902 , C12N2795/10322 , C12N2800/108 , C12N2800/208 , C12N2800/30 , C12N2830/00 , C12N2830/15 , C12N2830/40 , C12N2830/60 , C12N2830/85 , C12N2840/20 , C12N2840/203 , C12Q1/6897 , G01N2500/10
摘要： Artificial chromosomes, including ACes, that have been engineered to contain available sites for site-specific, recombination-directed integration of DNA of interest are provided. These artificial chromosomes provide tractable, efficient and rational engineering of the chromosome for a variety of applications.
摘要翻译：提供了人造染色体，包括ACes，其被设计成包含可用的位点，用于目标DNA的重组导向整合。这些人造染色体为各种应用提供了易于处理，有效和合理的染色体工程。

6. 发明申请

US20060095984A1 Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes 失效
公开(公告)号：US20060095984A1
公开(公告)日：2006-05-04
申请号：US11284877
申请日：2005-11-21
申请人： Gyula Hadlaczky , Aladar Szalay
发明人： Gyula Hadlaczky , Aladar Szalay
IPC分类号： A01H1/00 , C12N15/82
CPC分类号： C12N15/625 , A01K67/0275 , A01K2217/05 , A01K2217/072 , A01K2217/20 , A01K2227/105 , A01K2227/30 , A01K2267/01 , A01K2267/02 , A01K2267/025 , A01K2267/03 , A61K38/00 , A61K48/00 , C07K2319/036 , C07K2319/61 , C12N15/113 , C12N15/82 , C12N15/85 , C12N15/8509 , C12N2310/111 , C12N2800/20 , C12N2800/206 , C12N2800/208
摘要： Methods for amplification of nucleic acids in cells are provided. Also provided are cells that contain the nucleic acids.

7. 发明申请

US20020160970A1 Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes 审中-公开
标题翻译：人造染色体，其用途和制备人造染色体的方法
公开(公告)号：US20020160970A1
公开(公告)日：2002-10-31
申请号：US09799462
申请日：2001-03-05
发明人： Gyula Hadlaczky , Aladar A. Szalay
IPC分类号： A61K048/00 , C12N015/87
CPC分类号： C12N15/625 , A01K67/0275 , A01K2217/05 , A01K2217/072 , A01K2227/105 , A01K2227/30 , A01K2267/02 , A01K2267/025 , A01K2267/03 , A61K38/00 , A61K48/00 , C07K2319/036 , C07K2319/61 , C12N15/113 , C12N15/82 , C12N15/85 , C12N15/8509 , C12N2310/111 , C12N2800/20 , C12N2800/204 , C12N2800/206 , C12N2800/208
摘要： Methods for preparing cell lines that contain artificial chromosomes, methods for preparation of artificial chromosomes, methods for purification of artificial chromosomes, methods for targeted insertion of heterologous DNA into artificial chromosomes, methods for amplification of nucleic acids and methods for delivery of the chromosomes to selected cells and tissues are provided. Also provided are cell lines for use in the methods, and cell lines and chromosomes produced by the methods. Methods for use of the artificial chromosomes are also provided.6
摘要翻译：用于制备含有人造染色体的细胞系的方法，人造染色体的制备方法，纯化人造染色体的方法，将异源DNA靶向插入人工染色体的方法，扩增核酸的方法和将染色体递送至所选择的方法提供细胞和组织。还提供了用于方法的细胞系，以及通过该方法产生的细胞系和染色体。还提供了使用人造染色体的方法

8. 发明授权

US6133503A Mammalian artificial chromosomes and methods of using same 失效
标题翻译：哺乳动物人造染色体及其使用方法
公开(公告)号：US6133503A
公开(公告)日：2000-10-17
申请号：US24472
申请日：1998-02-17
申请人： Immo E. Scheffler
发明人： Immo E. Scheffler
IPC分类号： C12N15/85 , C12N15/00
CPC分类号： C12N15/85 , C12N2800/208 , C12N2800/30 , C12N2830/00 , C12N2830/42 , C12N2830/85 , C12N2840/203
摘要： The present invention provides a mammalian artificial chromosome (MAC), comprising a centromere and a unique cloning site, said MAC containing less than 0.1% of the DNA present in a normal haploid genome of the mammalian cell from which the centromere was obtained. The invention further provides a MAC, wherein the unique cloning site is a nucleic acid sequence encoding a selectable marker. The invention also provides methods of preparing a MAC. In addition, the invention provides methods of stably expressing a selectable marker in a cell, comprising introducing a MAC containing the selectable marker into the cell. The invention also provides a cell containing a MAC expressing an exogenous nucleic acid sequence and a transgenic mammal expressing a selectable marker.
摘要翻译：本发明提供了一种哺乳动物人造染色体（MAC），其包含着丝粒和唯一的克隆位点，所述MAC含有存在于获得着丝粒的哺乳动物细胞的正常单倍体基因组中存在的DNA的0.1％以下。本发明还提供MAC，其中所述唯一克隆位点是编码可选择标记的核酸序列。本发明还提供了制备MAC的方法。此外，本发明提供了在细胞中稳定表达选择标记的方法，包括将含有可选择标记的MAC引入细胞。本发明还提供含有表达外源核酸序列的MAC的细胞和表达可选择标记的转基因哺乳动物。

9. 发明授权

US09315824B2 Human artificial chromosome vector 有权
标题翻译：人类人造染色体载体
公开(公告)号：US09315824B2
公开(公告)日：2016-04-19
申请号：US13510327
申请日：2010-11-17
申请人： Yoshimi Kuroiwa , Hiroaki Matsushita , Akiko Sano
发明人： Yoshimi Kuroiwa , Hiroaki Matsushita , Akiko Sano
IPC分类号： C12N15/00 , C07H21/02 , C12P21/00 , A01K67/027 , C12N15/85 , C07K16/00 , C07K16/12
CPC分类号： A01K67/0278 , A01K2207/15 , A01K2217/052 , A01K2217/072 , A01K2227/101 , A01K2267/01 , C07K16/00 , C07K16/1278 , C12N15/85 , C12N15/8509 , C12N2800/208
摘要： The present invention provides a human artificial chromosome vector comprising a gene encoding the human antibody heavy chain, a gene encoding the human antibody light chain, and a gene encoding IgM heavy chain constant region derived from a nonhuman animal; and being capable of producing a human antibody with a higher efficiency when the vector is introduced into an animal. By immunizing the animal produced using a human artificial chromosome vector of the present invention with a desired antigen, a large quantity of human polyclonal antibodies can be supplied.
摘要翻译：本发明提供了包含编码人抗体重链的基因，编码人抗体轻链的基因和编码来自非人动物的IgM重链恒定区的基因的人类人造染色体载体，并且当将载体导入动物时能够产生效率更高的人抗体。通过使用本发明的人人造染色体载体用所需抗原免疫动物，可以提供大量的人多克隆抗体。

10. 发明授权

US08951789B2 Mammalian artificial chromosome vector comprising human cytochrome P450 gene (cluster) and non-human mammalian animal retaining the same 有权
标题翻译：包含人细胞色素P450基因（簇）和非人哺乳动物的哺乳动物人造染色体载体保持相同
公开(公告)号：US08951789B2
公开(公告)日：2015-02-10
申请号：US12743065
申请日：2008-10-14
申请人： Mitsuo Oshimura , Yasuhiro Kazuki , Takashi Matsuoka , Kazuma Tomizuka , Takeshi Oshima
发明人： Mitsuo Oshimura , Yasuhiro Kazuki , Takashi Matsuoka , Kazuma Tomizuka , Takeshi Oshima
IPC分类号： C12N15/63 , C12N5/06 , C12N15/85 , A01K67/027 , C12N9/02 , G01N33/50 , A01K67/00
CPC分类号： C12N15/8509 , A01K67/0278 , A01K2217/00 , A01K2217/07 , A01K2227/105 , C12N9/0071 , C12N9/0077 , C12N2800/208 , G01N33/5088
摘要： This invention relates to a mammalian artificial chromosome vector, which retains a human chromosome 7 fragment comprising human cytochrome P450 genes and is transmittable to progeny, wherein the human chromosome 7 fragment retains a region of approximately 1 Mb±500 Kb in size comprising at least a human CYP3A gene cluster, which region is located between chromosome markers AC004922 and AC073842, and to a non-human mammalian animal retaining the vector.
摘要翻译：本发明涉及一种哺乳动物人造染色体载体，其保留包含人细胞色素P450基因的人染色体7片段，并可传播给子代，其中人染色体7片段保留大小约1Mb±500Kb的区域，其包含至少一个人类CYP3A基因簇，该区域位于染色体标记物AC004922和AC073842之间，以及保留载体的非人哺乳动物。

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