专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20180030509A1 NUCLEIC ACID AMPLIFICATION REACTION METHOD, REAGENT, AND METHOD OF USING REAGENT 审中-公开
公开(公告)号：US20180030509A1
公开(公告)日：2018-02-01
申请号：US15650114
申请日：2017-07-14
申请人： Seiko Epson Corporation
发明人： Masato HANAMURA , Kotaro IDEGAMI , Masayuki UEHARA
IPC分类号： C12Q1/68 , G01N1/40 , G01N27/447
CPC分类号： C12Q1/686 , C12Q1/00 , C12Q1/6806 , C12Q1/6844 , C12Q1/6853 , G01N1/40 , G01N27/447 , G01N2001/4038 , C12Q2521/107 , C12Q2527/107 , C12Q2527/113
摘要： A nucleic acid amplification reaction method includes a heating step of heating a reaction solution containing a reverse transcriptase, a polymerase, a primer, and a probe for performing a reverse transcription reaction, and a thermal cycling step of performing thermal cycling for amplifying a nucleic acid for the reaction solution after the heating step, wherein the Tm value of the primer is 65° C. or higher and 80° C. or lower, and in the heating step, a heating time for the reaction solution is 5 seconds or more and 480 seconds or less.

2. 发明授权

US09714447B2 Detection of nucleic acid amplification in a porous substrate 有权
公开(公告)号：US09714447B2
公开(公告)日：2017-07-25
申请号：US13970315
申请日：2013-08-19
申请人： General Electric Company
发明人： Erin Jean Finehout , Christopher Michael Puleo , Ralf Lenigk , Jason M. Nichols , John Nelson
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q1/6816 , C12Q1/6851 , C12Q2565/137 , C12Q2565/625 , C12Q2527/113 , C12Q2565/133 , C12Q2545/114
摘要： The present disclosure relates to characterization of biological samples by amplification detection in a porous substrate. By way of example, a porous substrate may include amplification reagents configured to provide a signal when released during amplification. When a sample is applied, amplification occurs as a wavefront from the application point, and the time that the wavefront reaches a distance on the porous substrate is related to an initial concentration of the sample applied. By detecting the distance traveled by the amplification products at one or more time points, an initial concentration of the sample may be estimated.

3. 发明授权

US09678056B2 Control of enzyme translocation in nanopore sequencing 有权
公开(公告)号：US09678056B2
公开(公告)日：2017-06-13
申请号：US15366849
申请日：2016-12-01
申请人： Pacific Biosciences of California, Inc.
发明人： Stephen Turner , Benjamin Flusberg
IPC分类号： B82Y15/00 , G01N33/487 , C12Q1/68 , G01N27/447
CPC分类号： G01N33/48721 , B82Y5/00 , B82Y15/00 , C12Q1/6869 , C12Q1/6874 , C12Q2565/631 , G01N27/447 , G01N27/44791 , C12Q2563/116 , C12Q2527/113
摘要： The invention relates to devices and methods for nanopore sequencing. Methods for controlling translocation of through the nanopore are disclosed. The rate of transport of the template nucleic acids through the nanopore can be controlled using a translocating enzyme having two slow kinetic steps. The translocating enzyme and reaction conditions can be selected such that the translocating enzyme exhibits two kinetic steps wherein each of the kinetic steps has a rate constant, and the ratio of the rate constants of the kinetic steps is from 10:1 to 1:10. The invention also provides for using the signals from n-mers to provide sequence information, for example where the system has less than single base resolution. The invention includes arrays of nanopores having incorporated electronic circuits, for example, in CMOS.

4. 发明申请

US20170122929A1 CONTROL OF ENZYME TRANSLOCATION IN NANOPORE SEQUENCING 审中-公开
公开(公告)号：US20170122929A1
公开(公告)日：2017-05-04
申请号：US15366849
申请日：2016-12-01
申请人： Pacific Biosciences of California, Inc.
发明人： Stephen Turner , Benjamin Flusberg
IPC分类号： G01N33/487 , G01N27/447 , C12Q1/68
CPC分类号： G01N33/48721 , B82Y5/00 , B82Y15/00 , C12Q1/6869 , C12Q1/6874 , C12Q2565/631 , G01N27/447 , G01N27/44791 , C12Q2563/116 , C12Q2527/113
摘要： The invention relates to devices and methods for nanopore sequencing. Methods for controlling translocation of through the nanopore are disclosed. The rate of transport of the template nucleic acids through the nanopore can be controlled using a translocating enzyme having two slow kinetic steps. The translocating enzyme and reaction conditions can be selected such that the translocating enzyme exhibits two kinetic steps wherein each of the kinetic steps has a rate constant, and the ratio of the rate constants of the kinetic steps is from 10:1 to 1:10. The invention also provides for using the signals from n-mers to provide sequence information, for example where the system has less than single base resolution. The invention includes arrays of nanopores having incorporated electronic circuits, for example, in CMOS.

5. 发明申请

US20170045428A1 BIOLOGICAL SAMPLE PREPARATION FOR TESTING 审中-公开
标题翻译：生物样品制备测试
公开(公告)号：US20170045428A1
公开(公告)日：2017-02-16
申请号：US15233801
申请日：2016-08-10
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Mousumi RATH , Mark SHANNON , Jason LA
IPC分类号： G01N1/28 , C12Q1/68
CPC分类号： G01N1/04 , B01L3/50825 , B01L2300/042 , B01L2300/046 , B01L2300/0838 , C12Q1/6806 , G01N1/2813 , G01N1/286 , G01N1/34 , G01N2001/028 , G01N2001/045 , G01N2001/284 , G01N2001/2886 , C12Q2521/327 , C12Q2527/101 , C12Q2527/113 , C12Q2531/113 , C12Q2565/601 , C12Q2535/122
摘要： In one embodiment, a method for processing a sample includes selecting a selected sample from a biological specimen, the selected sample being in contact with a first surface of a first substrate. The method also includes transferring the selected sample directly from the first surface to a container comprising an internal volume. The method also includes forming or providing a sample solution within the internal volume of the container by contacting the selected sample with a lysis mixture using a protocol. The method further includes performing an assay, experiment, or test on the sample solution while the sample solution disposed is within the internal volume of the container.In another embodiment, a method for processing a sample includes providing a selected sample comprising one or more cells. The method also includes transferring the selected sample into an internal volume of a container. The method also includes contacting the selected sample with a lysis mixture using a protocol to provide a sample solution, wherein the protocol comprises heating the sample solution to a first temperature that is greater than 37 degrees Celsius and less than or equal to 75 degrees Celsius.
摘要翻译：在另一个实施方案中，用于处理样品的方法包括提供包含一个或多个细胞的选定样品。该方法还包括将所选择的样品转移到容器的内部容积中。该方法还包括使用方案使所选样品与裂解混合物接触以提供样品溶液，其中所述方案包括将样品溶液加热至大于37摄氏度且小于或等于75摄氏度的第一温度。

6. 发明申请

US20160304942A1 Enhanced Methods of Ribonucleic Acid Hybridization 审中-公开
标题翻译：增强核糖核酸杂交方法
公开(公告)号：US20160304942A1
公开(公告)日：2016-10-20
申请号：US15101676
申请日：2014-12-05
申请人： THE BROAD INSTITUTE, INC. , THE GENERAL HOSPITAL CORP(dba Massachusettts General Hospital)
发明人： Roby BHATTACHARYYA , Deb HUNG , Milesh PATEL
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , C12Q2523/113 , C12Q2527/101 , C12Q2527/113
摘要： This disclosure relates to a method for increasing the hybridization efficiency of a probe and a target RNA in a sample, for example to identify a particular RNA present in the sample. The method includes heating a lysate sample comprising at least one target RNA, such as a tRNA, mRNA or rRNA, at a temperature of about 95° C. for a time sufficient to interfere with secondary structure of the RNA, wherein the time is short enough, such that the RNA in the cell lysate sample are not significantly degraded, and wherein the lysate comprises a cell lysis buffer comprising a chemical denaturant. To detect a target RNA in the lysate, the lysate is contacted with at least one detectable probe, such as a labeled probe, designed to specifically hybridize to the target RNA in the lysate.
摘要翻译：本公开涉及提高样品中探针和靶RNA的杂交效率的方法，例如以鉴定样品中存在的特定RNA。该方法包括在大约95℃的温度下加热包含至少一种靶RNA（例如tRNA，mRNA或rRNA）的裂解物样品足以干扰RNA二级结构的时间，其中时间短足以使得细胞裂解物样品中的RNA不被显着降解，并且其中裂解物包含包含化学变性剂的细胞裂解缓冲液。为了检测裂解物中的靶RNA，将裂解物与设计为与裂解物中的靶RNA特异性杂交的至少一种可检测的探针（例如标记的探针）接触。

7. 发明授权

US09273303B2 Dried and stabilized ready-to-use composition containing nucleic acid polymerization enzymes for molecular biology applications 有权
标题翻译：干燥和稳定的即用型组合物含有用于分子生物学应用的核酸聚合酶
公开(公告)号：US09273303B2
公开(公告)日：2016-03-01
申请号：US13318835
申请日：2010-05-19
申请人： Ugo De Luca , Luigi Roveda , Maurizio Gramegna
发明人： Ugo De Luca , Luigi Roveda , Maurizio Gramegna
IPC分类号： C12Q1/68 , C12P19/34 , C12N9/96 , C12N9/12
CPC分类号： C12N9/96 , C12N9/1252 , C12Q1/6844 , C12Q2527/113 , C12Q2527/101 , C12Q2521/101
摘要： The present invention relates to a exsiccated or lyophilized composition comprising: a nucleic acid polymerization enzyme and cellobiose, in which the enzyme is stable for a period of time, even at a temperature of up to 55° C. The composition of the invention can also comprise further reagents, such as salts, primers specific for a template DNA present in a sample, probes, etc. The invention relates to a method for preparing an exsiccated or lyophilized composition comprising a nucleic acid polymerization enzyme and cellobiose, possibly in containers, in which the enzyme is lyophilized and ready for use in molecular biology applications upon addition of the sample.
摘要翻译：本发明涉及一种除草剂或冻干组合物，其包含：核酸聚合酶和纤维二糖，其中该酶在一段时间内是稳定的，甚至在最高达55℃的温度下。本发明的组合物也可以包含另外的试剂，例如盐，对样品中存在的模板DNA特异的引物，探针等。本发明涉及一种制备包含核酸聚合酶和纤维二糖的预滤液或冻干组合物的方法，可能在容器中，该酶在加入样品后被冻干并准备用于分子生物学应用。

8. 发明授权

US09200317B2 Nucleic acid amplification 有权
标题翻译：核酸扩增
公开(公告)号：US09200317B2
公开(公告)日：2015-12-01
申请号：US12737448
申请日：2009-07-13
申请人： Laurence Carlo Tisi , Olga Gandelman , Guy Kiddle , Cathal McElgunn
发明人： Laurence Carlo Tisi , Olga Gandelman , Guy Kiddle , Cathal McElgunn
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6848 , C12Q2545/101 , C12Q2531/101 , C12Q2527/107 , C12Q2527/113
摘要： A method for determining the presence and/or amount of a first polynucleic acid in a sample comprising subjecting the sample to nucleic acid amplification in which product is detectable by the presence of a signal generated by polynucleic acid formation from the first polynucleotide characterised in that the nucleic acid amplification reaction is conducted, in the same reaction vessel, with a predetermined amount of a second polynucleic acid which is subjected to nucleic acid amplification, the product of which is detectable by the presence of the same signal generated by polynucleic acid formation from the second polynucleotide as that generated by polynucleic acid formation from the first polynucleotide and wherein the product of the second polynucleic acid is produced with different reaction kinetics from the product of the first polynucleic acid such that the second polynucleic acid acts as an internal control for the method.
摘要翻译：一种用于确定样品中第一多核酸的存在和/或量的方法，包括使样品经受核酸扩增，其中可通过存在由第一多核苷酸形成的多核酸形成的信号检测产物，其特征在于，在相同的反应容器中进行核酸扩增反应，其中预定量的第二多核酸进行核酸扩增，其产物可以通过存在由多核酸形成产生的相同信号第二多核苷酸，其由来自第一多核苷酸的多核酸形成产生，并且其中第二多核酸的产物产生具有与第一多核酸产物不同的反应动力学，使得第二多核酸作为该方法的内部对照。

9. 发明授权

US09121064B2 Nanopore sequencing using n-mers 有权
标题翻译：使用n-mers进行纳孔测序
公开(公告)号：US09121064B2
公开(公告)日：2015-09-01
申请号：US14618385
申请日：2015-02-10
申请人： Pacific Biosciences of California, Inc.
发明人： Stephen Turner , Benjamin Flusberg
IPC分类号： C12Q1/68 , G01N33/48 , G01N33/487 , G01N27/447 , C12Q1/48
CPC分类号： G01N33/48721 , B82Y5/00 , B82Y15/00 , C12Q1/6869 , C12Q1/6874 , C12Q2565/631 , G01N27/447 , G01N27/44791 , C12Q2563/116 , C12Q2527/113
摘要： The invention relates to devices and methods for nanopore sequencing. The invention provides for using the signals from n-mers to provide sequence information, for example where the system has less than single base resolution. The invention includes arrays of nanopores having incorporated electronic circuits, for example, in CMOS. In some cases, the arrays of nanopores comprise resistive openings for isolating the electronic signals for improved sequencing. Methods for controlling translocation of through the nanopore are disclosed.
摘要翻译：本发明涉及纳米孔测序的装置和方法。本发明提供使用来自n-mers的信号来提供序列信息，例如系统具有小于单个基本分辨率的系统。本发明包括纳入电子电路的纳米孔阵列，例如在CMOS中。在一些情况下，纳米孔阵列包括用于隔离电子信号的电阻开口以改善测序。公开了通过纳米孔控制易位的方法。

10. 发明授权

US08895249B2 Kinetic exclusion amplification of nucleic acid libraries 有权
标题翻译：核酸文库的动力学排除扩增
公开(公告)号：US08895249B2
公开(公告)日：2014-11-25
申请号：US13783043
申请日：2013-03-01
申请人： Illumina, Inc.
发明人： Min-Jui Richard Shen , Jonathan Mark Boutell , Kathryn M. Stephens , Mostafa Ronaghi , Kevin Gunderson , Bala Murali Venkatesan , M. Shane Bowen , Kandaswamy Vijayan
IPC分类号： C12Q1/68 , C12P19/34 , B01J19/00
CPC分类号： C12Q1/6848 , B01J19/0046 , B01J2219/00585 , B01J2219/00587 , B01J2219/00608 , B01J2219/00621 , B01J2219/00637 , B01J2219/00653 , B01J2219/00675 , B01J2219/00722 , C12Q1/6844 , C12Q1/6874 , C12Q2521/507 , C12Q2527/101 , C12Q2527/113 , C12Q2535/122 , C12Q2565/513 , C12Q2565/537
摘要： A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.
摘要翻译：一种方法，包括（a）提供包含位点阵列的扩增试剂和具有不同靶核酸的溶液; 和（b）使扩增试剂反应以产生每个具有来自溶液的靶核酸的扩增子的克隆群的扩增位点。反应可以包括以平均传输速率同时将核酸输送到位点，以及以平均扩增速率扩增转运到位点的核酸，其中平均扩增速率超过平均传输速率。该反应可以包括从转运到每个位点的核酸产生第一扩增子，并产生来自核酸或第一扩增子的后续扩增子，其中后续扩增子产生的平均速率超过在其中生成第一个扩增子。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式