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1. 发明授权

US09963667B2 Structured biological samples for analysis by mass cytometry 有权
公开(公告)号：US09963667B2
公开(公告)日：2018-05-08
申请号：US14986431
申请日：2015-12-31
申请人： FLUIDIGM CANADA INC.
发明人： Alexander V. Loboda
IPC分类号： C12M1/12 , G01N1/28 , G01N33/483 , H01J49/00 , H01J49/04 , G01N1/04
CPC分类号： C12M25/00 , G01N1/286 , G01N33/4833 , G01N2001/045 , G01N2001/2886 , H01J49/0027 , H01J49/04
摘要： Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer or mass spectrometer.

2. 发明申请

US20170212020A1 Embedding Medium for Biological Samples, Method for Producing Embedded Biological Samples, and Use Thereof 审中-公开
公开(公告)号：US20170212020A1
公开(公告)日：2017-07-27
申请号：US15319611
申请日：2015-06-19
申请人： Medizinische Hochschule Hannover , Laser Zentrum Hannover e.V.
发明人： Mark Kuehnel , Manuela Kellner , Danny Jonigk , Nicole Izykowski , Heiko Meyer , Raoul-Amadeus Lorbeer , Marko Heidrich
IPC分类号： G01N1/36 , G01N1/28 , C12Q1/68 , G01N1/30 , G01N1/44
CPC分类号： G01N1/36 , C12Q1/68 , G01N1/286 , G01N1/30 , G01N1/44 , G01N2001/2886 , G01N2001/305 , G01N2001/364 , G06K9/00134 , G06K9/0014
摘要： In a first aspect, the invention relates to the use of a UV-polymerizable composition as an embedding medium for biological samples. The UV-polymerizable composition is a composition with a refractive index ranging from n=1.45 to n=1.6 after a polymerization process. In another aspect, the invention relates to a method for producing embedded biological samples using said UV-polymerizable composition and to the embedded biological samples themselves. The biological samples can be used in a wide variety of areas, such as diagnostics among others, and are suitable in particular for RNA-based diagnostics.

3. 发明授权

US09664599B2 Laser microdissection method and laser microdissection device 有权
公开(公告)号：US09664599B2
公开(公告)日：2017-05-30
申请号：US14005575
申请日：2012-03-21
申请人： Michael Gögler
发明人： Michael Gögler
IPC分类号： B23K26/38 , B23K26/40 , G01N1/28 , B23K26/00 , B23K103/00
CPC分类号： G01N1/286 , B23K26/0006 , B23K26/38 , B23K2103/30 , G01N2001/2886 , G05B2219/45041
摘要： Methods and apparatuses for laser microdissection are provided. For example, by a user at least one first system parameter is adjusted, for example varied, and at least one second system parameter of the laser microdissection system is adjusted automatically by the laser microdissection system such that a cut line has a desired cut line parameter.

4. 发明申请

US20160305855A1 METHOD AND APPARATUS FOR EXTRACTING AND COLLECTING SINGLE CELLS FROM FORMALIN-FIXED PARAFFIN EMBEDDED TISSUES 有权
标题翻译：从FORMALIN-FIXED PARAFFIN嵌入式组织中提取和收集单细胞的方法和装置
公开(公告)号：US20160305855A1
公开(公告)日：2016-10-20
申请号：US15099121
申请日：2016-04-14
申请人： Arizona Board of Regents, a body corporate of the State of Arizona, acting for and on behalf of
发明人： David Richardson , Thai Tran , Dmitry Derkach , Colleen Ziegler
IPC分类号： G01N1/28
CPC分类号： G01N1/286 , B01L3/5027 , B01L3/502715 , B01L2200/025 , B01L2200/0647 , B01L2300/0822 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2400/0688 , G01N2001/2886
摘要： Methods and apparatuses are provided for extraction and collection of sets of one or more cells from a formalin-fixed paraffin embedded tissue sample. A laser is used to remove sets of one or more cells from an immobilized sample within a microfluidic device, and a fluid flow element washes the removed set of one or more cells away from the immobilized sample, preferably to be collected in a downstream sample collection device. Morphological and/or positional information may be preserved to permit at least one technique (e.g., amplification, sequencing, and/or analytical, optionally performed after cells are lysed downstream of the microfluidic device) to be related to the original spatial location of the set of one or more cells in the immobilized sample.
摘要翻译：提供了用于从福尔马林固定石蜡包埋的组织样品中提取和收集一组或多种细胞的方法和装置。使用激光器从微流体装置内的固定化样品中去除一组或多种细胞组，并且流体流动元件将去除的一个或多个细胞组合从固定化样品中洗涤，优选收集在下游样品收集物设备。可以保留形态和/或位置信息以允许与组的原始空间位置相关的至少一种技术（例如，扩增，测序和/或分析，可选地在细胞裂解在微流体装置的下游之后进行）的固定化样品中的一个或多个细胞。

5. 发明申请

US20160194590A1 STRUCTURED BIOLOGICAL SAMPLES FOR ANALYSIS BY MASS CYTOMETRY 有权
标题翻译：通过大量细胞分析分析的结构生物样品
公开(公告)号：US20160194590A1
公开(公告)日：2016-07-07
申请号：US14986431
申请日：2015-12-31
申请人： FLUIDIGM CANADA INC.
发明人： Alexander V. Loboda
IPC分类号： C12M1/12 , G01N33/483 , H01J49/00 , G01N1/28
CPC分类号： C12M25/00 , G01N1/286 , G01N33/4833 , G01N2001/045 , G01N2001/2886 , H01J49/0027 , H01J49/04
摘要： Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer or mass spectrometer.
摘要翻译：公开了将生物样品递送到质谱仪的ICP源的装置和方法。生物材料设置在载体上的多个离散位置上。多个离散部位配置成保留生物材料并在施加能量时释放生物材料。载体位于气体导管附近，并且在从离散位置释放时，生物材料被夹带在气流中，其将生物材料的离散部分通过导管输送到ICP源以通过质量细胞计量分析。该装置和方法可以向质谱仪或质谱仪提供生物材料的分立部分的连续流。

6. 发明授权

US09279749B2 Laser microdissection method and apparatus 有权
标题翻译：激光显微解剖方法和装置
公开(公告)号：US09279749B2
公开(公告)日：2016-03-08
申请号：US14270275
申请日：2014-05-05
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Brian Donovan , Thomas Baer
IPC分类号： G01N1/28 , G01N1/44 , G01N1/30
CPC分类号： G01N1/28 , G01N1/2813 , G01N1/286 , G01N1/30 , G01N1/44 , G01N2001/2826 , G01N2001/2833 , G01N2001/284 , G01N2001/2886 , Y10T156/10 , Y10T156/1054
摘要： Systems and methods for automated laser microdissection are disclosed. In one variation, targeted biological material is manually or automatically selected and a transfer film is placed in juxtaposition to the location of an interior of a cut path. In another variation, a sample of biological material is mounted onto a polymer membrane which is then placed onto a substrate. Targeted biological material is manually or automatically selected and a transfer film is placed in juxtaposition with the targeted biological material on the side of the biological material. In yet another variation, a sample of biological material is mounted onto a polymer membrane which is then inverted onto a substrate. Targeted biological material is manually or automatically selected and a transfer film is placed in juxtaposition with the targeted biological material on the side of the polymer membrane. Then, an UV laser cuts along a cut path around the targeted portions of biological material in a closed cut path or a substantially closed cut path defining an interior and an exterior portion of the tissue sample. In a substantially closed cut path, bridges are left spanning the interior of the cut path and the exterior of the cut path. An IR laser activates at least a portion of the transfer film such that the transfer film in the vicinity of targeted portion adheres to the biological material interior to the cut path. The transfer film is then removed separating the targeted portions of biological material which are adhered to the transfer film from the remaining portion of the tissue sample.
摘要翻译：公开了用于自动激光显微切割的系统和方法。在一个变型中，手动或自动选择目标生物材料，并且将转移膜并排放置在切割路径的内部的位置。在另一个变体中，将生物材料样品安装在聚合物膜上，然后将其放置在基底上。手动或自动选择靶向生物材料，并将转移膜与生物材料侧面上的靶向生物材料并置放置。在另一个变体中，将生物材料样品安装在聚合物膜上，然后将其反转到基底上。手动或自动选择目标生物材料，并将转移膜与聚合物膜侧面上的目标生物材料并置放置。然后，UV激光沿着围绕生物材料的目标部分的切割路径沿封闭的切割路径或限定组织样本的内部和外部部分的基本封闭的切割路径切割。在基本封闭的切割路径中，跨越切割路径的内部和切割路径的外部。红外激光激活转印膜的至少一部分，使得目标部分附近的转印膜附着在生物材料内部至切割路径。然后除去转移膜，从生物材料的目标部分分离出粘附到转移膜上的剩余部分的组织样品。

7. 发明申请

US20160020069A1 TEM SAMPLE PREPARATION 有权
公开(公告)号：US20160020069A1
公开(公告)日：2016-01-21
申请号：US14869553
申请日：2015-09-29
申请人： FEI Company
发明人： Jeffrey Blackwood , Matthew Bray , Corey Senowitz , Cliff Bugge
IPC分类号： H01J37/305 , G01N1/28 , H01J37/26
CPC分类号： H01J37/3056 , G01N1/286 , G01N2001/2886 , H01J37/261 , H01J2237/31745
摘要： An improved method of preparing ultra-thin TEM samples that combines backside thinning with an additional cleaning step to remove surface defects on the FIB-facing substrate surface. This additional step results in the creation of a cleaned, uniform “hardmask” that controls the ultimate results of the sample thinning, and allows for reliable and robust preparation of samples having thicknesses down to the 10 nm range.

8. 发明授权

US09200989B2 Method and device for three dimensional microdissection 有权
标题翻译：三维显微解剖的方法和装置
公开(公告)号：US09200989B2
公开(公告)日：2015-12-01
申请号：US12256341
申请日：2008-10-22
申请人： Stefan Niehren
发明人： Stefan Niehren
IPC分类号： A01M1/02 , G01N1/28 , G01N1/06 , G01N1/42
CPC分类号： G01N1/2813 , G01N1/06 , G01N1/42 , G01N2001/2886
摘要： A method for three-dimensional microdissection for separating defined structures in the sub-millimeter range by cold laser ablation or multi-photon absorption, whereby exposure of the structures to be separated is performed using directional information in all spatial directions. Also, a 3D microdissection system for separating defined, three-dimensional structures from a sample, having: a control unit (5); an ablation chamber (1) with a sample holder, on which the sample to be processed is mounted and which is movable along a linear axis V and rotatable about a rotary axis R. The sample holder has positioning devices connected to the control unit. The positioning devices move the sample holder along another linear axis H and rotate it about rotary axis R, and a laser device (6) is introduced into the ablation chamber at least partially through a laser window (3) in the ablation chamber. The ablation chamber is connected to the control unit and has an adjustable optic, such that the laser beam is focused near the sample.
摘要翻译：用于通过冷激光烧蚀或多光子吸收在亚毫米范围内分离限定结构的三维显微解剖方法，由此使用所有空间方向上的方向信息来进行要分离的结构的曝光。另外，用于从样本中分离限定的三维结构的3D显微解剖系统，具有：控制单元（5）; 具有样品保持器的消融室（1），待处理的样品安装在该消融室（1）上，并且可沿线性轴线V移动并且可绕旋转轴线R旋转。样品架具有连接到控制单元的定位装置。定位装置沿着另一个线性轴线H移动样品架，并围绕旋转轴线R旋转，激光装置（6）至少部分地通过消融室中的激光窗（3）引入消融室。消融室连接到控制单元并且具有可调光学元件，使得激光束聚焦在样品附近。

9. 发明申请

US20150040688A1 SYSTEMS FOR TOUCHLESS PROCESSING OF DRIED BLOOD SPOTS AND METHODS OF USING SAME 审中-公开
标题翻译：用于无菌加工干燥血液的系统及其使用方法
公开(公告)号：US20150040688A1
公开(公告)日：2015-02-12
申请号：US14332225
申请日：2014-07-15
申请人： University of Washington through its Center for Commercialization
发明人： Sean C. Murphy
IPC分类号： G01N1/28 , G01N33/49
CPC分类号： G01N1/286 , B23K26/032 , B23K26/044 , B23K26/082 , B23K26/083 , B23K26/0869 , B23K26/16 , B23K26/38 , G01N2001/2886
摘要： The present technology relates generally to systems for processing dried blood spots (DBS) using laser cutting approaches to provide for rapid, contamination-free processing and methods of using same.
摘要翻译：本技术一般涉及使用激光切割方法处理干血斑（DBS）的系统，以提供快速，无污染的处理及其使用方法。

10. 发明授权

US08878147B2 Method and apparatus for in situ preparation of serial planar surfaces for microscopy 有权
标题翻译：用于显微镜的串联平面表面的原位制备方法和装置
公开(公告)号：US08878147B2
公开(公告)日：2014-11-04
申请号：US13745584
申请日：2013-01-18
申请人： Joseph C. Robinson
发明人： Joseph C. Robinson
IPC分类号： G21K5/10 , B23K15/00 , H01J37/26 , G01Q60/24 , G01Q30/20 , G02B21/26 , H01J37/20 , B82Y35/00 , G01N1/28
CPC分类号： B23K15/0006 , B82Y35/00 , G01N2001/2886 , G01Q30/20 , G01Q60/24 , G02B21/26 , H01J37/20 , H01J37/261 , H01J2237/2809 , H01J2237/31745
摘要： Systems and methods for preparing solid samples for analysis, such as microscopic examination in cross section or planimetric orientation. The sample preparation systems may include a sample support configured to secure a solid sample, an excitation beam source that generates an excitation beam configured to remove material from a surface of the sample, a beam shield configured to at least partially protect the sample from the excitation beam, and a beam shield holder configured to secure the beam shield, where the adjustment of the relative positions of the beam shield and sample holder permits the excitation beam to selectively expose a series of substantially planar surfaces of the sample.
摘要翻译：用于制备用于分析的固体样品的系统和方法，例如横截面或平面取向的显微镜检查。样品制备系统可以包括配置成固定固体样品的样品支撑体，产生被配置成从样品表面去除材料的激发束的激发束源，构造成至少部分地保护样品免受激发的束屏蔽光束和束屏蔽保持器，其构造成固定光束屏蔽，其中光束屏蔽和样品保持器的相对位置的调节允许激发光束选择性地暴露样品的一系列基本平坦的表面。

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