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1. 发明授权

US11578318B2 Large volume ex vivo electroporation method 有权
公开(公告)号：US11578318B2
公开(公告)日：2023-02-14
申请号：US15956103
申请日：2018-04-18
申请人： Cellectis S.A.
发明人： Richard E Walters , Alan D King
IPC分类号： C12N13/00 , C12M1/00 , C12N15/87 , C12M1/42 , C12M3/00 , C12M3/02 , H02K1/24 , H02K1/14 , H02K19/10 , A61K9/00
摘要： An object of the invention is to provide an electroporation method for treating vesicles with exogenous material for insertion of the exogenous material into the vesicles which includes the steps of: a. retaining a suspension of the vesicles and the exogenous material in a treatment volume in a chamber which includes electrodes, wherein the chamber has a geometric factor (cm−1) defined by the quotient of the electrode gap squared (cm2) divided by the chamber volume (cm3), wherein the geometric factor is less than or equal to 0.1 cm−1, wherein the suspension of the vesicles and the exogenous material is in a medium which is adjusted such that the medium has conductivity in a range spanning 50 microSiemens/cm to 500 microSiemens/cm, wherein the suspension is enclosed in the chamber during treatment, and b. treating the suspension enclosed in the chamber with one or more pulsed electric fields. With the method, the treatment volume of the suspension is scalable, and the time of treatment of the vesicles in the chamber is substantially uniform.

2. 发明授权

US11549097B2 Phase transfer of a cargo laden scaffold 有权
公开(公告)号：US11549097B2
公开(公告)日：2023-01-10
申请号：US16081305
申请日：2017-02-28
申请人： Oxford University Innovation Limited
发明人： Hagan Bayley , Sam Olof , Alexander D. Graham
IPC分类号： C12N5/00 , C12M1/26 , C12M1/12 , C12M3/02 , C12N5/16 , B33Y30/00 , A61L27/38 , A61L27/52
摘要： The present invention relates to a process for producing a composition comprising an aqueous medium and, disposed in the aqueous medium, a first volume of a first hydrogel, which process comprises: (i) providing a composition comprising a first hydrophobic medium and, disposed in the first hydrophobic medium, a first volume of a first hydrogel; (ii) disposing a volume of an aqueous composition comprising a hydrogel compound around the first volume of the first hydrogel; (iii) allowing the aqueous composition comprising the hydrogel compound to form a gel and thereby forming a hydrogel object, which hydrogel object comprises the first volume of the first hydrogel and a second volume of a second hydrogel, which second volume of the second hydrogel is disposed around the first volume of the first hydrogel; and (iv) transferring the hydrogel object from the first hydrophobic medium to an aqueous medium and thereby producing the composition comprising the aqueous medium and, disposed in the aqueous medium, the first volume of the first hydrogel. The invention further provides a hydrogel object, which hydrogel object comprises a first volume of a first hydrogel and a second volume of a second hydrogel, which second volume of the second hydrogel is disposed around the first volume of the first hydrogel.

3. 发明申请

US20190031990A1 METHOD FOR DISSOCIATING CELL AGGREGATES 审中-公开
公开(公告)号：US20190031990A1
公开(公告)日：2019-01-31
申请号：US16072379
申请日：2017-01-25
申请人： CENTRE FOR COMMERCIALIZATION OF REGENERATIVE MEDICINE
发明人： Nicholas Timmins , Lesley Chan
IPC分类号： C12M3/08 , C12M1/33 , C12M3/02 , C12M1/00 , C12N5/0735
摘要： A method for dissociating cell aggregates in an agitated reactor. The method comprises providing a cell culture comprising cell aggregates in the agitated reactor, contacting the cell aggregates with a dissociation reagent, generating a dissociation force in the agitated reactor and exposing the contacted cell aggregates to the generated dissociation force under conditions sufficient to dissociate the contacted cell aggregates. The method may be used in a process for passaging cells and/or generating dissociated differentiated cells from stem and/or progenitor cells.

4. 发明授权

US10179898B2 Bioreactor for the cultivation of mammalian cells 有权
公开(公告)号：US10179898B2
公开(公告)日：2019-01-15
申请号：US15612769
申请日：2017-06-02
申请人： Lonza Biologics PLC
发明人： Mohsan Khan
IPC分类号： C12M3/02 , C12M1/00 , C12M1/06 , C12N5/00 , C12M3/00
摘要： The present invention relates to large-scale bioreactors having at least two impellers, large-scale bioreactor systems and methods for the large scale cultivation and propagation of mammalian cells using these bioreactors.

5. 发明申请

US20180237733A1 CULTURE DEVICE FOR TISSUE CELL SUSPENSION 审中-公开
公开(公告)号：US20180237733A1
公开(公告)日：2018-08-23
申请号：US15752580
申请日：2016-08-09
申请人： CHONGQING RUNZE PHARMACEUTICAL CO., LTD.
发明人： Lei Ye
IPC分类号： C12M3/02 , C12M1/12 , C12N5/00 , C12M1/24 , C12M3/06 , C12M3/04
CPC分类号： C12M3/02 , C12M3/04 , C12M21/08 , C12M23/08 , C12M23/16 , C12M25/14 , C12M25/18 , C12M27/04 , C12N5/0062 , C12N5/0068
摘要： The present invention relates to a culture device for tissue cell suspension. The culture device includes a tissue cell culture body. The tissue cell culture body is a porous material. The porous material is formed by cavities classified into different levels according to the pore size of material and cavity walls surrounding to form the cavities. A lower level of small cavities is provided to surround the cavity wall which forms the upper level of large cavity. Cavities of each level are in communication with each other, and cavities between respective levels are also in communication with each other. The culture device further includes a swirler device provided therein. Such culture device can particularly facilitate normal and unrestricted growth of the suspension of cells in three-dimensional space, obstructing the formation of over-dense cell region or nutrient-rich region during the cell culturing.

6. 发明授权

US08895289B2 Method and device for photochemical process 有权
标题翻译：光化学过程的方法和装置
公开(公告)号：US08895289B2
公开(公告)日：2014-11-25
申请号：US12865270
申请日：2009-01-27
申请人： Martin Mohr , Franz Emminger
发明人： Martin Mohr , Franz Emminger
IPC分类号： C12N1/12 , C12M3/02 , C12M1/09 , C12M1/00
CPC分类号： C12M27/20 , C12M21/02 , C12M23/50
摘要： Photochemical process and device adapted to breed, produce, or hydrocultivate microorganisms. The process includes conveying a reaction medium in a reactor in a meander-shaped way that includes moving the reaction medium along a direction that perpendicularly or inclined at an angle to an imaginary horizontal plane, wherein, during the conveying, the reaction medium moves in the reactor at least once along a first direction defined as one of a top down direction and a direction of gravity, moves in the reactor at least once along a second direction defined as one of a bottom up direction and against the direction of gravity, and moves in the reactor one of freely under atmospheric pressure and while exposed to the atmosphere. The process also includes introducing into and removing from the reactor the reaction medium in a continuous manner.
摘要翻译：光化学过程和设备适于繁殖，生产或水培培养微生物。该方法包括在反应器中以曲折形式输送反应介质，其包括使反应介质沿着与假想水平面成一定角度的垂直或倾斜的方向移动，其中，在输送过程中，反应介质在反应器沿着被定义为上下方向和重力方向之一的第一方向至少一次，沿着被定义为下向上方向和重力方向之一的第二方向在反应器中移动至少一次，并且移动在反应器中一个在大气压力下自由的同时暴露在大气中。该方法还包括以连续方式引入反应器和从反应器中除去反应介质。

7. 发明授权

US08163536B2 Device for preparing an undifferentiated cell from a more committed cell 有权
标题翻译：用于从更致命的细胞制备未分化细胞的装置
公开(公告)号：US08163536B2
公开(公告)日：2012-04-24
申请号：US09853188
申请日：2001-05-09
申请人： Ilham Mohamed Saleh Saeed Abuljadayel
发明人： Ilham Mohamed Saleh Saeed Abuljadayel
IPC分类号： A01N1/02 , C12M3/02
CPC分类号： C12M41/14 , A61K48/00 , A61K2035/124 , C12M23/04 , C12M23/26 , C12M27/02 , C12M41/48 , C12N5/0647 , C12N2500/14 , C12N2500/24 , C12N2501/14 , C12N2501/22 , C12N2501/39 , C12N2501/50 , C12N2506/11 , C12N2510/00
摘要： A device for preparing an undifferentiated cell, the device comprises means for contacting a more committed cell with an agent that causes the more committed cell to retrodifferentiate into an undifferentiated cell.
摘要翻译：一种用于制备未分化细胞的装置，所述装置包括用于使更致命的细胞与导致更有承诺的细胞逆分化成未分化细胞的药剂接触的装置。

8. 发明申请

US20110165677A1 METHOD FOR REDUCING DEPOSITS DURING THE CULTIVATION OF ORGANISMS 审中-公开
标题翻译：用于在有机体生长过程中减少沉积物的方法
公开(公告)号：US20110165677A1
公开(公告)日：2011-07-07
申请号：US13062556
申请日：2009-09-17
申请人： Helmut Brod , Björn Frahm , Joerg Kauling
发明人： Helmut Brod , Björn Frahm , Joerg Kauling
IPC分类号： C12N5/02 , C12M3/02
CPC分类号： B01F3/04269 , B01F11/0088 , B01F2003/04319 , B01F2003/04404 , C12M23/24 , C12M27/04 , C12M29/04 , C12M39/00
摘要： The invention relates to a process for reducing the amount of deposits during the culture of organisms, and in particular of cell cultures, where these have a tendency towards agglomeration or adhesion to the bioreactor and to its elements, or where these are readily susceptible to agglomeration or adhesion of cell debris or of substances.
摘要翻译：本发明涉及一种减少生物体培养期间沉积物量的方法，特别是细胞培养物，其中这些沉积物具有聚集或粘附到生物反应器及其元件的趋势，或者其中容易易于聚集的方法或细胞碎片或物质的粘附。

9. 发明申请

US20110039322A1 METHOD FOR PRODUCING BIOGAS 审中-公开
标题翻译：生物生物学方法
公开(公告)号：US20110039322A1
公开(公告)日：2011-02-17
申请号：US12680002
申请日：2008-09-22
申请人： Urs Inauen
发明人： Urs Inauen
IPC分类号： C12P5/02 , C12M3/02
CPC分类号： C12M23/56 , B01F13/0049 , B01F13/0052 , C12M21/04 , C12M27/18 , Y02E50/343
摘要： The invention relates to a method for producing biogas, wherein biomass (B) is filled into a biogas reactor (R), and a plurality of mixing bodies (1) is disposed on the biomass (B). The mixing bodies (1) float on the surface (O) of the biomass (B) and thereby dip partially into the biomass (B). The surface of the biomass is thus mixed, leading to an increase in the efficiency of gas yield.
摘要翻译：本发明涉及一种生产沼气的方法，其中生物质（B）被填充到生物气反应器（R）中，并且多个混合体（1）设置在生物质（B）上。混合体（1）漂浮在生物质（B）的表面（O）上，从而部分浸入生物质（B）中。因此生物质的表面被混合，导致气体产率的效率的提高。

10. 发明申请

US20100055765A1 SEMI-CLOSED LOOP ALGA-DIESEL FUEL PHOTOBIOREACTOR USING WASTE WATER 失效
标题翻译：使用废水的半封闭式ALGA-柴油燃料光电子器件
公开(公告)号：US20100055765A1
公开(公告)日：2010-03-04
申请号：US12549656
申请日：2009-08-28
申请人： David L. Frank
发明人： David L. Frank
IPC分类号： C12M3/02 , C12N1/12
CPC分类号： C12M39/00 , C12M21/02 , C12M23/06 , C12M31/10 , Y02E50/13
摘要： A semi-closed loop diesel photobioreactor system and method are provided for producing diesel fuel from wild algae or from specialized algae that has been biologically modified for high efficiency oil production using waste water as a primary food source. The diesel photobioreactor provides a semi-closed loop system with an opening to acquire waste water below the surface to obtain waste water nutrients and to protect the algae species from contamination. The semi-closed loop diesel photobioreactor includes a container that can be designed in a variety of shapes with a tube design preferred, and containing a liquid culture medium for cultivating photosynthetic organisms. The system can utilize natural light and can also deploy an innovative lighting system integrated into the photobioreactor container. The diesel photobioreactor system also has one or more cleaning devices mounted within the container for cleaning the surface of the photobioreactor container.
摘要翻译：提供了一种半闭环柴油光生物反应器系统和方法，用于从野生藻类或已经用生物修饰用于利用废水作为主要食物源的高效油生产的专用藻类生产柴油。柴油光生物反应器提供了具有开口的半闭环系统，以获得表面下的废水以获得废水营养物质并保护藻类免受污染。半闭环柴油光生物反应器包括可以设计成各种形状的容器，其优选的是管设计，并且包含用于培养光合生物的液体培养基。该系统可以利用自然光，并且还可以部署集成到光生物反应器容器中的创新照明系统。柴油光生物反应器系统还具有安装在容器内的一个或多个清洁装置，用于清洁光生物反应器容器的表面。

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