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1. 发明授权

US09121027B2 Enhanced transformation of recalcitrant monocots 有权
标题翻译：强化转基因不育单子叶植物
公开(公告)号：US09121027B2
公开(公告)日：2015-09-01
申请号：US13455751
申请日：2012-04-25
申请人： Qiudeng Que , David Nicholl
发明人： Qiudeng Que , David Nicholl
IPC分类号： C12N15/82 , C12N15/84 , C12N9/90
CPC分类号： C12N15/8205 , C12N9/90 , C12N15/821 , C12N15/8245 , C12N15/8246 , C12Y503/01008
摘要： Provided herein are improved methods for transforming monocotyledonous plants, as well as an improved phosphomannose-isomerase (PMI) protein coding region and transformation vectors including the same.
摘要翻译：本文提供了用于转化单子叶植物以及改进的磷酸甘露糖异构酶（PMI）蛋白质编码区和包含其的转化载体的改良方法。

2. 发明授权

US08592212B2 Soybean transformation method 有权
公开(公告)号：US08592212B2
公开(公告)日：2013-11-26
申请号：US13245599
申请日：2011-09-26
申请人： Brian J. Martinell , Lori S. Julson , Carol A. Emler , Yong Huang , Dennis E. McCabe , Edward J. Williams
发明人： Brian J. Martinell , Lori S. Julson , Carol A. Emler , Yong Huang , Dennis E. McCabe , Edward J. Williams
IPC分类号： C12N15/84 , C12N15/82
CPC分类号： C12N15/8205
摘要： A method is disclosed for the Agrobacterium-mediated germline genetic transformation of soybean. The method is based on Agrobacterium-mediated gene delivery to individual cells in a freshly germinated soybean meristem, which cells can be induced directly to form shoots that give rise to transgenic plants. This method does not involve callus-phase tissue culture and is rapid and efficient.

3. 发明授权

US08581045B2 Hypervirulent mutant of Agrobacterium tumefaciens 有权
标题翻译：根癌土壤杆菌的无毒突变体
公开(公告)号：US08581045B2
公开(公告)日：2013-11-12
申请号：US12950833
申请日：2010-11-19
申请人： Stephen K. Farrand , David Michael Barnhart
发明人： Stephen K. Farrand , David Michael Barnhart
IPC分类号： C12N15/84 , C12N1/21 , A01H5/10 , C12R1/41
CPC分类号： C12N15/8205 , C12N9/88
摘要： This disclosure relates to a mutant Agrobacterium tumefaciens that is functionally deleted for the atu1060 gene that codes for the cyclic di-GMP synthase Atu1060, as well as methods for its use in transforming plants with desired transgenes. Such bacteria are more virulent than currently used strains of A. tumefaciens, and thus can be used to transform a wider variety of plants, such as plants that are traditionally recalcitrant to such transformation.
摘要翻译：本公开涉及对编码环状二-GMP合酶Atu1060的atu1060基因功能缺失的根癌土壤杆菌突变体，以及其用于转化具有所需转基因植物的方法。这种细菌比目前使用的根瘤土壤杆菌菌株更具毒性，因此可用于转化更广泛种类的植物，例如传统上顽固地转化为这种转化的植物。

4. 发明授权

US08507758B2 Markerless transformation 有权
标题翻译：无标记变换
公开(公告)号：US08507758B2
公开(公告)日：2013-08-13
申请号：US13335646
申请日：2011-12-22
申请人： Joseph Michael Palys
发明人： Joseph Michael Palys
IPC分类号： C12N15/82 , C12N15/84 , C12N15/10 , C12N15/32
CPC分类号： C12Q1/6895 , C12N15/8201 , C12N15/8205 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12N15/8286 , Y02A40/162
摘要： Methods for identification of successful transformation without a need for in vitro selection are provided. Direct detection of nucleotide sequences of interest is described which eliminate the need for use of ancillary nucleotide sequences.
摘要翻译：提供了不需要体外选择的用于鉴定成功转化的方法。描述了目的核苷酸序列的直接检测，其消除了使用辅助核苷酸序列的需要。

5. 发明授权

US08415527B2 DNA constructs and methods to enhance the production of commercially viable transgenic plants 有权
公开(公告)号：US08415527B2
公开(公告)日：2013-04-09
申请号：US12542586
申请日：2009-08-17
申请人： Larry A. Gilbertson , Elysia K. Krieger , Wanggen Zhang , Xudong Ye
发明人： Larry A. Gilbertson , Elysia K. Krieger , Wanggen Zhang , Xudong Ye
IPC分类号： C12N15/84 , C12N15/53 , A01H1/04
CPC分类号： C12N15/821 , C12N15/8212
摘要： The present invention has incorporated a non-lethal negative selectable marker gene into the vector backbone DNA of a DNA plasmid used to transform plant cells. These transgenes are designed to express a non-lethal gene product in plant cells that contain the vector backbone DNA of the DNA plasmid. The gene products of the non-lethal negative selectable marker gene are involved in plant hormone biosynthesis pathways, plant hormone substrate diversion, plant hormone degradation, plant hormone signaling or metabolic interference. The use of these DNA plasmids to transform plant cells provides for the enhanced production of commercially viable plants.

6. 发明授权

US08404930B2 Methods for improving monocot transformation 失效
标题翻译：改善单子叶植物转化的方法
公开(公告)号：US08404930B2
公开(公告)日：2013-03-26
申请号：US12694013
申请日：2010-01-26
申请人： Xinli E. Wu , Myeong-Je Cho , Zuo-Yu Zhao
发明人： Xinli E. Wu , Myeong-Je Cho , Zuo-Yu Zhao
IPC分类号： C12N15/84 , A01H4/00
CPC分类号： C12N15/8205
摘要： The invention provides improved plant transformation methods. In particular the method provides increased transformation frequency, especially in recalcitrant plants. The method includes various transformation protocols for monocots, such as maize and sorghum, using a combination of media and light conditions to achieve increased efficiency of monocot transformation and increased callus initiation frequencies.
摘要翻译：本发明提供改进的植物转化方法。特别地，该方法提供增加的转化频率，特别是在顽固植物中。该方法包括用于单子叶植物（例如玉米和高粱）的各种转化方案，其使用培养基和光照条件的组合来实现提高单子叶植物转化的效率和增加的愈伤组织起始频率。

7. 发明授权

US08343767B2 Methods for increasing homologous recombination of a nucleic acid sequences 失效
标题翻译：增加核酸序列同源重组的方法
公开(公告)号：US08343767B2
公开(公告)日：2013-01-01
申请号：US13434694
申请日：2012-03-29
申请人： Paul Harris , Howard Brody
发明人： Paul Harris , Howard Brody
IPC分类号： C12N15/00 , C12N1/15 , C12N15/64 , C12N15/84
CPC分类号： C12P21/00 , C12N15/80 , C12N15/902
摘要： The present invention relates to methods for increasing homologous recombination of a nucleic acid sequence introduced into a host cell, comprising: (a) introducing into a population of filamentous fungal host cells a first nucleic acid sequence encoding a recombination protein and a second nucleic acid sequence comprising one or more regions which are homologous with the genome of the filamentous fungal host cell, wherein (i) the recombination protein promotes the recombination of the one or more regions with the corresponding homologous region in the host's genome to incorporate the second nucleic acid sequence by homologous recombination, and (ii) the number of host cells comprising the incorporated second nucleic acid sequence in the population is increased at least 20% compared to the same population without the first nucleic acid sequence; (b) and isolating from the population a filamentous fungal cell comprising the incorporated second nucleic acid sequence.
摘要翻译：本发明涉及用于增加引入宿主细胞的核酸序列的同源重组的方法，其包括：（a）向丝状真菌宿主细胞群体中引入编码重组蛋白和第二核酸序列的第一核酸序列包括与丝状真菌宿主细胞的基因组同源的一个或多个区域，其中（i）重组蛋白促进一个或多个区域与宿主基因组中的相应同源区域的重组，以掺入第二核酸序列通过同源重组，和（ii）与没有第一核酸序列的相同群体相比，包含所述群体中掺入的第二核酸序列的宿主细胞的数量增加至少20％; （b）并从群体中分离包含掺入的第二核酸序列的丝状真菌细胞。

8. 发明授权

US08273954B1 Genetically transformed plants 失效
标题翻译：转基因植物
公开(公告)号：US08273954B1
公开(公告)日：2012-09-25
申请号：US06793486
申请日：1985-10-30
申请人： Stephen G. Rogers , Robert B. Horsch , Robert T. Fraley
发明人： Stephen G. Rogers , Robert B. Horsch , Robert T. Fraley
IPC分类号： C12N15/84 , C12N15/54
CPC分类号： C12N15/8205
摘要： This invention relates to genetically transformed, non-tumorous plant cells. A modified Ti plasmid is created which contains a left T-DNA border, one or more desired genes, and a right T-DNA border. This region does not contain tumorigenic or phytohormone-altering genes. The Ti plasmid is inserted into plant cells, where the T-DNA region is transferred into the plant genome. The transformed plant cells may be regenerated into morphologically normal plants which will pass the desired gene(s) to their descendants.
摘要翻译：本发明涉及遗传转化的非肿瘤植物细胞。产生修饰的Ti质粒，其包含左T-DNA边界，一个或多个所需基因和右T-DNA边界。该区域不含有致瘤性或植物激素改变基因。将Ti质粒插入到植物细胞中，其中T-DNA区被转移到植物基因组中。转化的植物细胞可以再生成形态上正常的植物，其将通过所需基因到其后代。

9. 发明授权

US08207399B2 Production of syringyl lignin in gymnosperms 失效
标题翻译：在裸子植物中生产丁香木质素
公开(公告)号：US08207399B2
公开(公告)日：2012-06-26
申请号：US12801785
申请日：2010-06-24
申请人： Vincent L. Chiang , Daniel T. Carraway , Richard H. Smeltzer
发明人： Vincent L. Chiang , Daniel T. Carraway , Richard H. Smeltzer
IPC分类号： C12N15/82 , C12N15/84
CPC分类号： C12N9/0077 , C12N9/1007 , C12N9/93 , C12N15/8222 , C12N15/8223 , C12N15/8255
摘要： The present invention relates to a method for producing syringyl lignin in gymnosperms. The production of syringyl lignin in gymnosperms is accomplished by genetically transforming a gymnosperm genome, which does not normally contain genes which code for enzymes necessary for production of syringyl lignin, with DNA which codes for enzymes found in angiosperms associated with production of syringyl lignin. The expression of the inserted DNA is mediated using host promoter regions in the gymnosperm. In addition, genetic sequences which code for gymnosperm lignin anti-sense mRNA may be incorporated into the gymnosperm genome in order to suppress the formation of the less preferred forms of lignin in the gymnosperm such as guaiacyl lignin
摘要翻译：本发明涉及一种在裸子植物中生产丁香木素的方法。在裸子植物中生产丁香木质素通过遗传转化裸子植物基因组来实现，该裸体植物基因组通常不含有编码用于生产丁香木质素所必需的酶的基因，其编码与在生长丁香木质素相关的被子植物中发现的酶。插入的DNA的表达使用裸子植物中的宿主启动子区域进行介导。此外，编码裸子植物木质素反义mRNA的遗传序列可以并入裸子植物基因组中，以抑制裸子植物中更少优选的木质素形式，例如愈创木薯木质素

10. 发明授权

US08203032B2 Application of fluorescent protein to garden plant 有权
标题翻译：荧光蛋白在花园植物中的应用
公开(公告)号：US08203032B2
公开(公告)日：2012-06-19
申请号：US12374995
申请日：2007-07-25
申请人： Iwao Waga , Hiromi Takenaka , Shu Muto
发明人： Iwao Waga , Hiromi Takenaka , Shu Muto
IPC分类号： C12N15/82 , C12N15/84 , C12N15/12
CPC分类号： C12N15/8212 , C07K14/43509
摘要： The present invention provides a process for generation of a transformed plant capable of emitting fluorescence by introducing a gene encoding a non-plant-derived fluorescent protein into a plant such that the fluorescent protein is recombinantly expressed in the active form of its mature protein in the leaf or petal of the plant, and also provides a transformed garden plant capable of emitting fluorescence that is generated by using the process. For example, cDNA encoding the full-length amino acid sequence of a Chiridius poppei-derived fluorescent protein CpYGFP or its H52F modified protein CpYGFP H52F is inserted into a T-DNA-based expression vector system, which is in turn introduced into the chromosomal DNA of a plant. As a result, the transformed plant thus generated can exhibit fluorescence attributed to these fluorescent proteins and exhibit no substantial difference in the other phenotypes from wild-type one of the plant.
摘要翻译：本发明提供一种通过将编码非植物来源的荧光蛋白的基因导入植物而产生能够发射荧光的转化植物的方法，使得荧光蛋白以其成熟蛋白的活性形式重组表达植物的叶或花瓣，并且还提供能够发射通过使用该过程产生的荧光的转化的花园植物。例如，将编码Chiridius Poppei衍生的荧光蛋白CpYGFP或其H52F修饰的蛋白质CpYGFP H52F的全长氨基酸序列的cDNA插入到基于T-DNA的表达载体系统中，其进而导入染色体DNA 的植物。结果，由此产生的转化植物可以表现出归因于这些荧光蛋白的荧光，并且在野生型植物中的其它表型中没有显示实质性差异。

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