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1. 发明授权

US11542514B2 Apparatus for the preparation and use of plant embryo explants for transformation 有权
公开(公告)号：US11542514B2
公开(公告)日：2023-01-03
申请号：US17124115
申请日：2020-12-16
申请人： Monsanto Technology LLC
发明人： Beth Jo Calabotta , Kevin Lee Deppermann , Erik D. Dersch , Jerald D. Heise , Angela Ranae Koestel , Cindy L. Ludwig , Brian J. Martinell , Edward Williams
IPC分类号： C12N15/82 , C12N5/00 , A01H4/00 , C12N5/04 , G01N33/00 , C12N9/10 , A01H6/20 , A01H6/54 , A01H6/46 , A01H6/60 , C12N1/20 , C12R1/41
摘要： The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

2. 发明授权

US09586870B2 Competitive and effective bacterial strains 有权
标题翻译：竞争有效的细菌菌株
公开(公告)号：US09586870B2
公开(公告)日：2017-03-07
申请号：US14636829
申请日：2015-03-03
申请人： Novozymes Biologicals, Inc.
发明人： Yaowei Kang , Shawn Semones , Jessica Smith , Kristi Woods
IPC分类号： A01N43/16 , A01N43/90 , A01N63/02 , C05F11/08 , A01N63/00 , C12R1/41 , C12R1/01
CPC分类号： C05F11/08 , A01N43/16 , A01N43/90 , A01N63/00 , A01N63/02 , C12R1/01 , C12R1/41
摘要： According to the present invention new isolates of bacterial strains have been shown to possess unique properties. These bacterial strains are plant growth-promoting rhizobacterium (PGPR), posses an enhanced competitive advantage at colonizing leguminous plants, and enhance the overall performance of leguminous plant growth. Further still, the present invention discloses a novel method for screening and selecting bacterial strains having the aforementioned beneficial characteristics.
摘要翻译：根据本发明，细菌菌株的新分离物已显示具有独特的性质。这些细菌菌株是植物生长促进的根瘤菌（PGPR），在定植豆科植物方面具有更强的竞争优势，并提高豆科植物生长的整体表现。此外，本发明还公开了一种筛选和选择具有上述有益特征的细菌菌株的新方法。

3. 发明授权

US09394562B2 Soil microorganism, novel oxidoreductase separated from the soil microorganism, gene encoding the oxidoreductase, and method for producing aglycones using the microorganism, the oxidoreductase and the gene 有权
标题翻译：土壤微生物，从土壤微生物分离的新型氧化还原酶，编码氧化还原酶的基因，以及使用微生物，氧化还原酶和基因产生糖苷配基的方法
公开(公告)号：US09394562B2
公开(公告)日：2016-07-19
申请号：US14588790
申请日：2015-01-02
申请人： AMOREPACIFIC CORPORATION , SNU R&DB Foundation
发明人： Jun Seong Park , Duck Hee Kim , Han Kon Kim , Eun Mi Kim , Byung Gee Kim
IPC分类号： C12Q1/26 , C12P33/20 , C12N9/02 , C12P17/06 , C12P19/02 , C12P33/00 , C12R1/41 , C12N1/20
CPC分类号： C12P33/20 , C12N1/20 , C12N9/0004 , C12P17/06 , C12P19/02 , C12P33/00 , C12R1/41 , Y02P20/52
摘要： The present invention relates to the novel Rhizobium sp. GIN611 KCTC11708BP or to cell extracts thereof, to a novel oxidoreductase which exhibits a glycolytic activity, to a gene encoding the oxidoreductase, to a recombinant strain comprising recombinant vector proteins or to an expression vector encoding recombinant proteins, and to a method for the glycolysis of natural products using same as a biocatalyst. The present invention also relates to a method for producing aglycones from a variety of natural products using same. The novel oxidoreductase separated from the novel microorganism of the present invention does not belong to a glucosidase group but belongs to an oxidoreductase group, and has a glycolytic activity for natural products. The novel oxidoreductase oxidizes the sugar in the aglycones of natural products, thereby producing a variety of aglycones.
摘要翻译：本发明涉及新根瘤菌属（Rhizobium sp。） GIN611KCTC11708BP或其细胞提取物，对具有糖酵解活性的新型氧化还原酶，与编码氧化还原酶的基因相连，包含重组载体蛋白的重组菌株或编码重组蛋白的表达载体，以及糖酵解方法使用与生物催化剂相同的天然产物。本发明还涉及使用它们的各种天然产物制备糖苷配基的方法。从本发明的新型微生物分离的新型氧化还原酶不属于葡萄糖苷酶基团，属于氧化还原酶基团，对天然产物具有糖酵解活性。新型氧化还原酶氧化天然产物的糖苷配基中的糖，从而产生各种糖苷配基。

4. 发明授权

US09365859B2 Use of non-agrobacterium bacterial species for plant transformation 有权
标题翻译：使用非农杆菌菌种进行植物转化
公开(公告)号：US09365859B2
公开(公告)日：2016-06-14
申请号：US14298739
申请日：2014-06-06
申请人： Monsanto Technology LLC
发明人： Xudong Ye , Junjiang Shen , Edward Williams
IPC分类号： C12N15/82 , C12R1/41
CPC分类号： C12N15/8202 , C12N15/8205 , C12R1/41 , G01N33/5097
摘要： The invention relates to methods for Rhizobia-mediated genetic transformation of plant cells, including soybean, canola, corn, and cotton cells. These include both VirD2-dependent and VirD2-independent methods. Bacterial species utilized include strains of Rhizobium sp., Sinorhizobium sp., and Mesorhizobium sp. Vectors for use in such transformation are also disclosed.
摘要翻译：本发明涉及用于根瘤菌介导的植物细胞遗传转化的方法，包括大豆，卡诺拉油菜，玉米和棉花细胞。这些包括依赖于VirD2和与VirD2无关的方法。使用的细菌种类包括根瘤菌属，中华根瘤菌属（Sinorhizobium sp。）和中生根瘤菌属（Mesorhizobium sp。还公开了用于这种转化的载体。

5. 发明授权

US09102972B2 Microorganism Rhizobium sp. KB10 having properties of promoting growth of Botryococcus braunii and increasing fatty acid content 有权
标题翻译：微生物根瘤菌属 KB10具有促进葡萄球菌生长的特性，增加脂肪酸含量
公开(公告)号：US09102972B2
公开(公告)日：2015-08-11
申请号：US13816735
申请日：2013-01-11
申请人： Korea Research Institute of Bioscience and Biotechnology
发明人： Hee Mock Oh , Chi Yong Ahn , Young Ki Lee , So Ra Ko , Hee Sik Kim
IPC分类号： C12P39/00 , C10L1/18 , C12N1/12 , C12P7/64 , C12R1/41 , C12N1/38 , C10L1/02
CPC分类号： C12P39/00 , C10L1/026 , C10L1/1802 , C10L2200/0476 , C10L2290/26 , C12N1/12 , C12N1/38 , C12P7/64 , C12P7/6409 , C12P7/6427 , C12R1/41 , Y02E50/13
摘要： The present invention relates to Rhizobium sp. KB10 strain having properties of promoting growth of Botryococcus braunii, which is an alga capable of producing biodiesel, and also enhancing production performance of biodiesel. Mores specifically, it relates to novel Rhizobium sp. KB10 strain which has properties of promoting growth of Botryococcus braunii used for biodiesel production and also enhancing content of C18 (i.e., oleate) corresponding to high quality biodiesel component as much as 900%. By using root colonizing bacteria like Rhizobium, it is possible to promote effectively the slow cell growth of Botryococcus braunii and increase as much as possible the oleate amount, which is a high quality biodiesel component. Further, by carrying out mixture culture using such bacteria, problems associated with contamination by other microorganisms during a process of producing biodiesel by culture in an outside environment can be dramatically solved.
摘要翻译：本发明涉及根瘤菌属具有促进生产生物柴油的藻类Botryococcus braunii生长的特性的KB10菌株，以及提高生物柴油的生产性能。具体来说，它涉及新根瘤菌属 KB10菌株具有促进用于生物柴油生产的Botryococcus braunii生长的性质，并且还提高对应于高质量生物柴油成分的C18（即油酸酯）含量高达900％。通过使用根瘤菌等细菌根瘤菌，可以有效地促进布鲁氏菌（Botryococcus braunii）的缓慢细胞生长，并尽可能地增加油酸盐量，这是高品质的生物柴油成分。此外，通过使用这种细菌进行混合培养，可以显着地解决与在外部环境下通过培养生产生物柴油的过程中由其他微生物污染相关的问题。

6. 发明授权

US08772576B2 Herbicide-resistant inoculant strains 有权
标题翻译：除草剂抗性接种菌株
公开(公告)号：US08772576B2
公开(公告)日：2014-07-08
申请号：US13393869
申请日：2010-09-03
申请人： L. David Kuykendall
发明人： L. David Kuykendall
IPC分类号： C12R1/41
CPC分类号： C12R1/01 , A01N63/00 , C12N1/20
摘要： Exemplary embodiments are directed to a herbicide resistant N2-fixing microorganism and to a method for enhancing N2 fixation by the herbicide resistant rhizobia in symbiosis with herbicide resistant or tolerant leguminous plants treated with herbicide. At least one preferred embodiment comprises mutant rhizobia strains selected based on tolerance of the broad spectrum glyphosate weed control agent, originally Roundup®. Preferred embodiments can be expected to outcompete both indigenous soil rhizobia and/or commercial rhizobia that are not resistant to the herbicide.
摘要翻译：示例性实施方案涉及除草剂抗性N2固定微生物和通过除草剂抗性根瘤菌与除草剂抗除草剂抗性或耐受性豆科植物共生的N 2固定的方法。至少一个优选实施方案包括基于广谱草甘膦除草剂的耐受性选择的突变根瘤菌菌株，最初为Roundup。预期优选的实施方案可以胜过对除草剂不具有抗性的土着土壤根瘤菌和/或商业根瘤菌。

7. 发明授权

US5840548A Process for producing microbial lipids from hydrocarbons 失效
标题翻译：从碳氢化合物生产微生物脂质的方法
公开(公告)号：US5840548A
公开(公告)日：1998-11-24
申请号：US527335
申请日：1995-09-12
申请人： Kimihiko Ogihara , Masamichi Sato , Kazunori Ishigami , Kouki Sakashita , Yoshihiro Takayama
发明人： Kimihiko Ogihara , Masamichi Sato , Kazunori Ishigami , Kouki Sakashita , Yoshihiro Takayama
IPC分类号： A61K8/31 , A61K8/99 , A61Q7/00 , A61Q19/00 , C11B1/10 , C12N1/28 , C12P7/64 , C12R1/01 , C12R1/06 , C12R1/13 , C12R1/15 , C12R1/32 , C12R1/41 , C12P7/62
CPC分类号： C12P7/6463 , A61K8/31 , A61K8/99 , A61Q19/00 , A61Q7/00
摘要： The present invention relates to a process for producing lipids, which comprises culturing bacteria capable of assimilating aliphatic hydrocarbons and accumulating lipids in medium containing aliphatic hydrocarbons until lipids are accumulated in the bacteria and then recovering the lipids therefrom, as well as to process for producing cosmetics which comprises using the lipids produced by said method.
摘要翻译：本发明涉及一种生产脂质的方法，其包括培养能够吸收脂肪烃并在含有脂肪族烃的培养基中积聚脂质的细菌，直到脂质积聚在细菌中，然后从其中回收脂质，以及生产化妆品的方法其包括使用由所述方法产生的脂质。

8. 发明授权

US5759824A Genes for butyrobetaine/crotonobetaine-l-carnitine metabolism and their use for the microbiological production of l-carnitine 失效
标题翻译：丁基甜菜碱/巴豆甜菜碱-1-肉碱代谢基因及其用于生产左旋肉碱的微生物
公开(公告)号：US5759824A
公开(公告)日：1998-06-02
申请号：US615191
申请日：1996-07-02
申请人： Thomas Zimmermann , Josef Werlen
发明人： Thomas Zimmermann , Josef Werlen
IPC分类号： C12N15/09 , C12N1/21 , C12N15/52 , C12N15/74 , C12P13/00 , C12R1/01 , C12R1/41 , C07H21/04 , C12N1/20
CPC分类号： C12P13/007 , C12N15/52 , C12N15/743
摘要： An isolated DNA fragment including the genes bcoC, bcoA/B, bcoD coding for the enzymes for L-carnitine biosynthesis in .gamma.-butyrobetaine/crotonobetaine metabolism and, additionally, including the gene bcoT coding for a potential transport protein in .gamma.-butyrobetaine/crotonobetaine metabolism. Also, any vector which includes an isolated DNA fragment including the genes bcoC, bcoA/B, bcoD coding for the enzymes for L-carnitine biosynthesis in .gamma.-butyrobetaine/crotonobetaine metabolism and, additionally, including the gene bcoT coding for a potential transport protein in .gamma.-butyrobetaine/crotonobetaine metabolism. Also, biotechnological process for the production of L-carnitine characterized in that crotonobetaine and/or .gamma.-butyrobetaine is fermented in the presence of a suitable carbon and nitrogen source using a microorgansim and L-carnitine is isolated.
摘要翻译： PCT No.PCT / EP94 / 03317 371日期：1996年7月2日 102（e）日期1996年7月2日PCT 1994年10月7日PCT PCT。公开号WO95 / 10613 日期1995年4月20日一个分离的DNA片段，包括编码γ-丁基甜菜碱/巴豆甜菜碱代谢中左旋肉碱生物合成酶的基因bcoC，bcoA / B，bcoD，另外还包括编码潜在转运蛋白的基因bcoT γ-丁酸甜菜碱/巴豆甜菜碱代谢。此外，任何载体包括分离的DNA片段，其包括编码γ-丁酸碱/巴豆甜菜碱代谢中L-肉碱生物合成的酶的基因bcoC，bcoA / B，bcoD，另外还包括编码潜在转运蛋白的基因bcoT 在γ-丁酮丁酸/巴豆甜菜碱代谢中。而且，用于生产L-肉毒碱的生物技术方法，其特征在于在合适的碳源和氮源的存在下使用微生物素和L-肉碱分离发酵巴豆甜菜碱和/或γ-丁酸甜菜碱。

9. 发明授权

US5530195A Bacillus thuringiensis gene encoding a toxin active against insects 失效
标题翻译：苏云金芽孢杆菌基因编码对昆虫有活性的毒素
公开(公告)号：US5530195A
公开(公告)日：1996-06-25
申请号：US257999
申请日：1994-06-10
申请人： Vance C. Kramer , Thomas C. Currier
发明人： Vance C. Kramer , Thomas C. Currier
IPC分类号： A01H5/00 , A01N63/00 , C07K14/325 , C12N1/19 , C12N1/21 , C12N5/10 , C12N7/00 , C12N15/00 , C12N15/09 , C12N15/32 , C12N15/82 , C12R1/01 , C12R1/07 , C12R1/38 , C12R1/41 , C12R1/91 , C12R1/92 , A01H4/00
CPC分类号： A01N63/00 , C07K14/325 , C12N15/8286
摘要： The present invention is drawn to a novel toxin gene purified and isolated from Bacillus thuringiensis var kurstaki and given the designation CryIE(c). The novel toxin gene encodes a protein of approximately 130 kDa in size and is active against Lepidopteran insects. Also included in the invention are the proteins encoded by CryIE(c). Further disclosed are recombinant genes and proteins derived from CryIE(c). Also provided are biologically pure bacterial strains transformed with the CryIE(c) gene which can be used in entomocidal formulations for the control of Lepidopteran insects. Yet another aspect of the invention are plants transformed with the toxin gene or active fragments thereof, particularly where the transforming sequences have been optimized for expression in maize.
摘要翻译：本发明涉及从苏云金芽孢杆菌var kurstaki纯化和分离的新型毒素基因，并命名为CryIE（c）。新型毒素基因编码大小约130kDa的蛋白质，对鳞翅目昆虫具有活性。本发明还包括由CryIE（c）编码的蛋白质。进一步公开的是来自CryIE（c）的重组基因和蛋白质。还提供了用CryIE（c）基因转化的生物学纯细菌菌株，其可用于控制鳞翅目昆虫的昆虫杀虫剂。本发明的另一方面是用毒素基因或其活性片段转化的植物，特别是其中转化序列已被优化用于在玉米中表达的植物。

10. 发明授权

US5302387A Bacillus thuringiensis isolates active against cockroaches and genes encoding cockroach-active toxins 失效
标题翻译：苏云金芽孢杆菌分离株抗蟑螂和编码蟑螂活性毒素的基因
公开(公告)号：US5302387A
公开(公告)日：1994-04-12
申请号：US958551
申请日：1992-10-19
申请人： Jewel M. Payne , M. Keith Kennedy , John B. Randall , David O. Brower
发明人： Jewel M. Payne , M. Keith Kennedy , John B. Randall , David O. Brower
IPC分类号： C12N15/09 , A01N63/00 , A01N63/02 , C07K14/325 , C12N1/20 , C12N1/21 , C12P21/02 , C12R1/01 , C12R1/02 , C12R1/05 , C12R1/065 , C12R1/07 , C12R1/18 , C12R1/22 , C12R1/38 , C12R1/41 , C12R1/425 , C12R1/465
CPC分类号： C12R1/075 , A01N63/00 , C07K14/325
摘要： The subject invention concerns a novel microbe and genes encoding novel toxin proteins with activity against cockroaches. Cockroaches are common house pests, and they create problems in hospitals, the food industry and in agriculture. The novel Bacillus thuringiensis microbe of the invention is referred to as B.t. PS185L8. The subject invention also concerns the use of PS201T6 to control cockroaches. The spores or crystals of these microbes, or variants thereof, are useful to control cockroaches in various environments. The genes of the invention can be used to transform various hosts wherein the novel toxic proteins can be expressed.
摘要翻译：本发明涉及新型微生物和编码具有抗蟑螂活性的新型毒素蛋白质的基因。蟑螂是常见的家蝇，它们在医院，食品工业和农业中造成问题。本发明的新型苏云金芽孢杆菌微生物称为B.t. PS185L8。本发明还涉及使用PS201T6来控制蟑螂。这些微生物的孢子或晶体或其变体可用于在各种环境中控制蟑螂。本发明的基因可用于转化其中可以表达新的有毒蛋白质的各种宿主。

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