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1. 发明申请

US20160319289A1 USE OF NON-AGROBACTERIUM BACTERIAL SPECIES FOR PLANT TRANSFORMATION 审中-公开
公开(公告)号：US20160319289A1
公开(公告)日：2016-11-03
申请号：US15151383
申请日：2016-05-10
申请人： MONSANTO TECHNOLOGY LLC
发明人： Xudong Ye , Junjiang Shen , Edward Williams
IPC分类号： C12N15/82 , G01N33/50 , C12R1/41
CPC分类号： C12N15/8202 , C12N15/8205 , C12R1/41 , G01N33/5097
摘要： The invention relates to methods for Rhizobia-mediated genetic transformation of plant cells, including soybean, canola, corn, and cotton cells. These include both VirD2-dependent and VirD2-independent methods. Bacterial species utilized include strains of Rhizobium sp., Sinorhizobium sp., and Mesorhizobium sp. Vectors for use in such transformation are also disclosed.

2. 发明申请

US20150184216A1 NOVEL SOIL MICROORGANISM, NOVEL OXIDOREDUCTASE SEPARATED FROM THE SOIL MICROORGANISM, GENE ENCODING THE OXIDOREDUCTASE, AND METHOD FOR PRODUCING AGLYCONES USING THE MICROORGANISM, THE OXIDOREDUCTASE AND THE GENE 审中-公开
标题翻译：新型土壤微生物，从土壤微生物分离的新型氧化还原酶，编码氧化菌的基因，以及使用微生物，氧化还原酶和基因产生雄烯的方法
公开(公告)号：US20150184216A1
公开(公告)日：2015-07-02
申请号：US14588790
申请日：2015-01-02
申请人： AMOREPACIFIC CORPORATION
发明人： Jun Seong PARK , Duck Hee KIM , Han Kon KIM , Eun Mi KIM , Byung Gee KIM
IPC分类号： C12P33/20 , C12P17/06
CPC分类号： C12P33/20 , C12N1/20 , C12N9/0004 , C12P17/06 , C12P19/02 , C12P33/00 , C12R1/41 , Y02P20/52
摘要： The present invention relates to the novel Rhizobium sp. GIN611 KCTC11708BP or to cell extracts thereof, to a novel oxidoreductase which exhibits a glycolytic activity, to a gene encoding the oxidoreductase, to a recombinant strain comprising recombinant vector proteins or to an expression vector encoding recombinant proteins, and to a method for the glycolysis of natural products using same as a biocatalyst. The present invention also relates to a method for producing aglycones from a variety of natural products using same. The novel oxidoreductase separated from the novel microorganism of the present invention does not belong to a glucosidase group but belongs to an oxidoreductase group, and has a glycolytic activity for natural products. The novel oxidoreductase oxidizes the sugar in the aglycones of natural products, thereby producing a variety of aglycones.
摘要翻译：本发明涉及新根瘤菌属（Rhizobium sp。） GIN611KCTC11708BP或其细胞提取物，对具有糖酵解活性的新型氧化还原酶，与编码该氧化还原酶的基因相连，包含重组载体蛋白的重组菌株或编码重组蛋白的表达载体，以及糖酵解方法使用与生物催化剂相同的天然产物。本发明还涉及使用它们的各种天然产物制备糖苷配基的方法。从本发明的新型微生物分离的新型氧化还原酶不属于葡萄糖苷酶基团，属于氧化还原酶基团，对天然产物具有糖酵解活性。新型氧化还原酶氧化天然产物的糖苷配基中的糖，从而产生各种糖苷配基。

3. 发明申请

US20140087420A1 NOVEL MICROORGANISM RHIZOBIUM SP. KB10 HAVING PROPERTIES OF PROMOTING GROWH OF BOTRYOCOCCUS BRAUNII AND INCREASING FATTY ACID CONTENT 有权
标题翻译：新型微生物RHIZOBIUM SP。 KB10具有促进白僵菌生长和增加脂肪酸含量的特性
公开(公告)号：US20140087420A1
公开(公告)日：2014-03-27
申请号：US13816735
申请日：2013-01-11
申请人： Hee Mock Oh , Chi Yong Ahn , Young Ki Lee , So Ra Ko , Hee Sik Kim
发明人： Hee Mock Oh , Chi Yong Ahn , Young Ki Lee , So Ra Ko , Hee Sik Kim
IPC分类号： C12P39/00 , C10L1/18
CPC分类号： C12P39/00 , C10L1/026 , C10L1/1802 , C10L2200/0476 , C10L2290/26 , C12N1/12 , C12N1/38 , C12P7/64 , C12P7/6409 , C12P7/6427 , C12R1/41 , Y02E50/13
摘要： The present invention relates to Rhizobium sp. KB10 strain having properties of promoting growth of Botryococcus braunii, which is an alga capable of producing biodiesel, and also enhancing production performance of biodiesel. Mores specifically, it relates to novel Rhizobium sp. KB10 strain which has properties of promoting growth of Botryococcus braunii used for biodiesel production and also enhancing content of C18 (i.e., oleate) corresponding to high quality biodiesel component as much as 900%. By using root colonizing bacteria like Rhizobium, it is possible to promote effectively the slow cell growth of Botryococcus braunii and increase as much as possible the oleate amount, which is a high quality biodiesel component. Further, by carrying out mixture culture using such bacteria, problems associated with contamination by other microorganisms during a process of producing biodiesel by culture in an outside environment can be dramatically solved.
摘要翻译：本发明涉及根瘤菌属具有促进生产生物柴油的藻类Botryococcus braunii生长的特性的KB10菌株，以及提高生物柴油的生产性能。具体来说，它涉及新根瘤菌属 KB10菌株具有促进用于生物柴油生产的Botryococcus braunii生长的性质，并且还提高了对应于高质量生物柴油成分高达900％的C18（即油酸酯）含量。通过使用根瘤菌等细菌根瘤菌，可以有效地促进布鲁氏菌（Botryococcus braunii）的缓慢细胞生长，并尽可能地增加油酸盐量，这是高品质的生物柴油成分。此外，通过使用这种细菌进行混合培养，可以显着地解决与在外部环境下通过培养生产生物柴油的过程中由其他微生物污染相关的问题。

4. 发明授权

US07824569B2 Soluble salt produced from a biopolymer and a process for producing the salt 有权
标题翻译：由生物聚合物生产的可溶性盐和生产盐的方法
公开(公告)号：US07824569B2
公开(公告)日：2010-11-02
申请号：US12243084
申请日：2008-10-01
申请人： John K. Newman , David B. Ringelberg , Kevin P. O'Connell , William A. Martin , Victor F. Medina , Steven L. Larson
发明人： John K. Newman , David B. Ringelberg , Kevin P. O'Connell , William A. Martin , Victor F. Medina , Steven L. Larson
IPC分类号： C09K3/22
CPC分类号： C05G3/04 , C09K17/32 , C12P19/04 , C12R1/41
摘要： Modifications to the extracellular polymeric substance, predominantly extracellular polysaccharide, of a biopolymer produced by Rhizobium tropici ATCC 49672 yield a stable dry salt transported more easily than a fluid or gel and more stable than either. The salt may be re-constituted with water on-site. Embodiments may be employed as a soil amendment for soil strengthening, reducing hydraulic conductivity, erosion control and dust control as well as a metal chelator for contaminant remediation. Based on comparison with dextran standards, an embodiment demonstrated a molecular weight over 511,000 D. Embodiments include a day salt that is precipitated from solution and in use is re-hydrated back to original form. When added to a sandy soil at 0.1% by dry weight, an embodiment decreased the hydraulic conductivity by three orders of magnitude. These properties make embodiments of the present invention an attractive, “green” alternative to petroleum-based synthetic polymers for such applications as rapid roadway construction and soil erosion prevention.
摘要翻译：对由根瘤菌根瘤菌ATCC 49672生产的生物聚合物的细胞外聚合物，主要是细胞外多糖的修饰产生比流体或凝胶更容易运输的稳定的干盐，并且比任一种更稳定。盐可以在现场用水重新构成。可以使用实施例作为土壤强化的土壤修正，降低水力传导性，侵蚀控制和粉尘控制以及用于污染物修复的金属螯合剂。基于与葡聚糖标准的比较，一个实施方案证实了分子量超过511,000D.实施方案包括从溶液中沉淀并在使用中再次水合回到原始形式的日盐。当以干重计0.1％添加到沙质土壤时，实施例将水力传导性降低三个数量级。这些性质使得本发明的实施方案成为用于诸如快速道路施工和防止水土流失的应用的石油基合成聚合物的有吸引力的“绿色”替代物。

5. 发明申请

US20100035347A1 METHOD FOR INCREASING THE SURVIVAL OF BACTERIAL STRAINS OF THE RHIZOBIUM GENUS 有权
标题翻译：增加生物碱菌株细菌菌株生长的方法
公开(公告)号：US20100035347A1
公开(公告)日：2010-02-11
申请号：US11917405
申请日：2006-06-13
申请人： Roberto Defez
发明人： Roberto Defez
IPC分类号： C12N15/74 , C12N1/20
CPC分类号： C12R1/41 , C12N1/06 , C12N1/20 , C12P17/10
摘要： The present invention describes a method for increasing the survival of the bacteria of Rhizobium genus, comprising the steps of: making the bacteria to grow in a chemically defined medium; keeping the bacteria in growth stationary phase for a proper period of time; exposing the bacteria to effective quantities of indole-3-acetic acid (IAA). Within the invention scope there is an alternative method to increase the survival of the bacteria of the Rhizobium genus by means of genetic engineering comprising the steps of: making a recombinant vector codifying enzymes able to produce IAA to express in effective way in said bacteria; making the bacteria to grow in chemically defined culture medium; keeping the bacteria in growth stationary phase for a proper period of time.
摘要翻译：本发明描述了一种提高根瘤菌属细菌存活率的方法，包括以下步骤：使细菌在化学上确定的培养基中生长; 保持细菌生长稳定期一段适当的时间; 将细菌暴露于有效量的吲哚-3-乙酸（IAA）。在本发明范围内，存在通过遗传工程增加根瘤菌属细菌存活率的替代方法，包括以下步骤：制备能够产生IAA的重组载体，以有效地在所述细菌中表达; 使细菌在化学定义的培养基中生长; 保持细菌生长稳定期一段适当的时间。

6. 发明申请

US20070249035A1 Surfactant biocatalyst for remediation of recalcitrant organics and heavy metals 有权
公开(公告)号：US20070249035A1
公开(公告)日：2007-10-25
申请号：US11810773
申请日：2007-06-07
申请人： Robin Brigmon , Sandra Story , Denis Altman , Christopher Berry
发明人： Robin Brigmon , Sandra Story , Denis Altman , Christopher Berry
IPC分类号： A62D3/02
CPC分类号： B09C1/10 , C12R1/01 , C12R1/05 , C12R1/40 , C12R1/41 , C12R1/425 , C12R1/44 , G21F9/18 , G21F9/28 , Y10S435/829 , Y10S435/874 , Y10S435/877 , Y10S435/878 , Y10S435/88 , Y10S435/882
摘要： Novel strains of isolated and purified bacteria have been identified which have the ability to degrade petroleum hydrocarbons including a variety of PAHs. Several isolates also exhibit the ability to produce a biosurfactant. The combination of the biosurfactant-producing ability along with the ability to degrade PAHs enhances the efficiency with which PAHs may be degraded. Additionally, the biosurfactant also provides an additional ability to bind heavy metal ions for removal from a soil or aquatic environment.

7. 发明授权

US6057146A Microorganisms for the stabilization of plasmids 失效
标题翻译：用于稳定质粒的微生物
公开(公告)号：US6057146A
公开(公告)日：2000-05-02
申请号：US239524
申请日：1999-01-29
申请人： Thomas Zimmermann , Cristiana Boraschi , Knut Burgdorf , Catherine Caubere
发明人： Thomas Zimmermann , Cristiana Boraschi , Knut Burgdorf , Catherine Caubere
IPC分类号： C12N1/21 , C12N15/00 , C12N15/09 , C12N15/11 , C12N15/52 , C12N15/65 , C12N15/68 , C12N15/74 , C12N1/20 , C12P13/04 , C12N21/06
CPC分类号： C12R1/41 , C12N15/68 , C12N15/74
摘要： New microorganisms with plasmids stable relative to betaine use. These new microorganisms contain (a) a hybrid plasmid with a DNA fragment that contains a genetic sequence that codes for the use of betaine and (b) a mutation in the chromosomal gene coding for the betaine use.
摘要翻译：质粒相对于甜菜碱使用的新型微生物稳定。这些新微生物含有（a）具有DNA片段的杂交质粒，该DNA片段含有编码使用甜菜碱的遗传序列，和（b）编码甜菜碱使用的染色体基因中的突变。

8. 发明授权

US5415672A Delivery of beneficial clavibacter microorganisms to seeds and plants 失效
标题翻译：将有益的棒状微生物递送到种子和植物
公开(公告)号：US5415672A
公开(公告)日：1995-05-16
申请号：US171774
申请日：1993-12-22
申请人： Jed W. Fahey , Joanna Anders
发明人： Jed W. Fahey , Joanna Anders
IPC分类号： A01G7/00 , A01C1/00 , A01C1/06 , C12N1/00 , A01C1/08 , A01N63/00
CPC分类号： C12R1/41 , A01C1/06 , C12N1/00 , C12R1/01
摘要： A seed containing beneficial microorganisms, which, upon germination gives rise to a plant colonized by the microorganisms, and a method for producing the seed are provided. The seed is produced by adding the microorganisms to a biologically compatible liquid carrier to form a suspension of the microorganisms in the carrier, impregnating the seeds with the suspension, and removing the excess carrier from the seeds, whereby the viability of the microorganisms and the seeds is maintained. Plants colonized by beneficial microorganisms and methods of producing such plants are also disclosed.
摘要翻译：提供含有有益微生物的种子，其在发芽时产生由微生物定植的植物，以及种子的生产方法。通过将微生物加入到生物相容的液体载体中以形成载体中微生物的悬浮液，用悬浮液浸渍种子，并从种子中除去过量的载体，从而产生微生物和种子的活力被维护。还公开了由有益微生物定植的植物和生产这种植物的方法。

9. 发明授权

US5077209A Dicarboxylic acid transport genes 失效
标题翻译：二羧酸转运基因
公开(公告)号：US5077209A
公开(公告)日：1991-12-31
申请号：US224609
申请日：1988-07-27
申请人： Fergal O'Gara
发明人： Fergal O'Gara
IPC分类号： C07K14/195 , C12N1/21 , C12N15/52 , C12N15/74
CPC分类号： C12R1/41 , C07K14/195 , C12N15/52 , C12N15/743
摘要： The invention relates to DNA fragments encoding genes which complement dicarboxylic acid transport mutants of Rhizobium species to plasmids encoding such DNA sequences and to bacterial hosts carrying the said plasmids. The invention further relates to a method of increasing dicarboxylic acid transport in Rhizobium species and to a method of increasing the nitrogen fixing ability of plants by infecting or inoculating the plants with bacterial hosts containing the above described DNA fragments.
摘要翻译：本发明涉及编码将根瘤菌物种的二羧酸转运突变体与编码这些DNA序列的质粒互补的携带所述质粒的细菌宿主的DNA片段。本发明还涉及增加根瘤菌物种中二羧酸转运的方法以及通过用含有上述DNA片段的细菌宿主感染或接种植物来提高植物的氮固定能力的方法。

10. 发明授权

US5045461A Method for increasing yield and nodulation by bradyrhizobium 失效
标题翻译：增加产量和结核杆菌的方法
公开(公告)号：US5045461A
公开(公告)日：1991-09-03
申请号：US213906
申请日：1988-06-30
申请人： Kieran F. Scott
发明人： Kieran F. Scott
IPC分类号： C05F11/08 , C07K14/195 , C12N1/11 , C12N1/21 , C12N15/74
CPC分类号： C12R1/41 , C05F11/08 , C07K14/195 , C12N15/743 , Y10S435/878
摘要： A method of increasing nodulation of a plant capable of being nodulated by Bradyrhizobium sp. (Parasponis) is provided comprising infecting said plant with a Bradyrhizobium sp. (Parasponia) species mutated such that nodK is non-functional. One such method of mutation involves insertion upstream of nodABC constitutive promoter sequences capable of activating nodABC. The invention is exemplified by the inoculation of siratro with mutated Bradyrhizobium sp. (Parasponia) mutated by insertion of nptII promoter sequences in nodK upstream of nodABC so as to activate nodABC. As compared with inoculation with the corresponding wild-type Bradyrhizobium sp. (Parasponia), nodulation onset occurred five days earlier, nodulation number was doubled, and a 120% enhancement of plant yield was shown. Similar results were obtained regardless of the orientation of the nptII promoter-containing sequences within the nodK coding sequence.
摘要翻译：一种能够增加由Bradyrhizobium sp。被结瘤的植物的结瘤的方法。（Parasponis）包括用Bradyrhizobium sp。感染所述植物。（Parasponia）物种突变，使得nodK不起作用。一种这样的突变方法涉及在能够激活nodABC的nodABC组成型启动子序列的上游插入。通过用突变的Bradyrhizobium sp接种siratro来举例说明本发明。（Parasponia）通过在nodAB上游的nodK中插入nptII启动子序列突变，以激活nodABC。与接种相应的野生型Bradyrhizobium sp相比，（Parasponia），结核发病发生在五天前，结瘤数增加一倍，植株产量提高了120％。无论nodK编码序列中含有nptII启动子的序列的取向如何，均获得相似的结果。

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