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1. 发明授权

US06835552B2 Impedance measurements for detecting pathogens attached to antibodies 失效
标题翻译：用于检测与抗体相关的病原体的阻抗测量
公开(公告)号：US06835552B2
公开(公告)日：2004-12-28
申请号：US09737542
申请日：2000-12-14
申请人： Robin R. Miles , Kodumudi S. Venkateswaran , Christopher K. Fuller
发明人： Robin R. Miles , Kodumudi S. Venkateswaran , Christopher K. Fuller
IPC分类号： G01N3353
CPC分类号： G01N33/5438 , G01N27/3276 , Y10S435/817 , Y10S436/806 , Y10S436/823
摘要： The use of impedance measurements to detect the presence of pathogens attached to antibody-coated beads. In a fluidic device antibodies are immobilized on a surface of a patterned interdigitated electrode. Pathogens in a sample fluid streaming past the electrode attach to the immobilized antibodies, which produces a change in impedance between two adjacent electrodes, which impedance change is measured and used to detect the presence of a pathogen. To amplify the signal, beads coated with antibodies are introduced and the beads would stick to the pathogen causing a greater change in impedance between the two adjacent electrodes.
摘要翻译：使用阻抗测量来检测与抗体包被的珠粒相连的病原体的存在。在流体装置中，将抗体固定在图案化交错电极的表面上。流经电极的样品流体中的病原体附着于固定的抗体，其产生两个相邻电极之间的阻抗变化，该阻抗变化被测量并用于检测病原体的存在。为了扩增信号，引入用抗体包被的珠粒，并且珠粒将粘附到病原体上，导致两个相邻电极之间的阻抗变化更大。

2. 发明授权

US06828109B2 Methods for detecting an analyte of interest using catalyzed reporter deposition of tyramide 有权
标题翻译：使用催化报道沉积酪胺来检测感兴趣分析物的方法
公开(公告)号：US06828109B2
公开(公告)日：2004-12-07
申请号：US09738049
申请日：2000-12-15
申请人： David R. Kaplan
发明人： David R. Kaplan
IPC分类号： G01N3353
CPC分类号： G01N33/56972 , G01N2001/302
摘要： The present invention relates to methods of staining cells for flow cytometry, using catalyzed reporter deposition and amplification staining. A catalyzed reporter deposition or an analyte dependent enzyme activation system is described for detecting and/or quantitating an analyte of interest in or on a cell by flow cytometry. Also described are compositions for use in catalyzed reporter deposition methods that can be used to reduce background staining, and thereby enhance peak signal separation between histograms obtained from cells stained with control immunoglobulin versus cells stained with immunoglobulin specific for an analyte of interest, in catalyzed deposition methods.
摘要翻译：本发明涉及使用催化报道沉淀和扩增染色染色细胞的流式细胞术的方法。描述了催化的报告沉积或分析物依赖性酶活化系统，用于通过流式细胞术检测和/或定量细胞中或细胞上的目标分析物。还描述了用于催化报道沉积方法的组合物，其可用于减少背景染色，从而增强从对照免疫球蛋白染色的细胞获得的直方图与感兴趣的分析物的免疫球蛋白染色的催化沉积中的峰信号分离方法。

3. 发明授权

US06824999B1 Detection of antibodies to gangliosides using solid-phase reactants coated with carbonyl groups 有权
标题翻译：使用用羰基涂覆的固相反应物检测神经节苷脂的抗体
公开(公告)号：US06824999B1
公开(公告)日：2004-11-30
申请号：US09565837
申请日：2000-05-05
申请人： Normand J. Robichaud , Louis P. Kertiles
发明人： Normand J. Robichaud , Louis P. Kertiles
IPC分类号： G01N3353
CPC分类号： G01N33/92 , G01N33/564 , G01N2405/10
摘要： Methods of detecting antibodies to one or more ganglioside(s) of interest in a sample are disclosed which comprise using a solid-phase reactant having carbonyl groups attached thereon, and the ganglioside(s) of interest linked to the solid-phase reactant by an amide bond between an amino group of the ganglioside of interest and a carbonyl group attached to the solid-phase reactant. The methods of detecting antibodies to ganglioside(s) of interest can be used in methods of diagnosing neuropathies in an individual.
摘要翻译：公开了检测样品中一种或多种目的神经节苷脂的抗体的方法，其包括使用其上连接有羰基的固相反应物和与固相反应物连接的神经节苷脂通过感兴趣的神经节苷脂的氨基与连接到固相反应物的羰基之间的酰胺键。检测感兴趣的神经节苷脂的抗体的方法可用于诊断个体神经病的方法。

4. 发明授权

US06824996B2 Production of ascorbic acid 失效
公开(公告)号：US06824996B2
公开(公告)日：2004-11-30
申请号：US10026139
申请日：2001-12-18
申请人： Manoj Kumar
发明人： Manoj Kumar
IPC分类号： G01N3353
CPC分类号： C12P7/04 , C12N1/16
摘要： The present invention provides for the production of ASA from yeast capable of producing ASA from KLG. The present invention provides methods for the production of ASA as well as recombinant yeast capable of producing ASA from a carbon source.

5. 发明授权

US06824989B1 Recombinant monoclonal antibody to phosphotyrosine-containing proteins 失效
标题翻译：含有磷酸酪氨酸的蛋白质的重组单克隆抗体
公开(公告)号：US06824989B1
公开(公告)日：2004-11-30
申请号：US09653755
申请日：2000-09-01
申请人： Dominic Eisinger , Lynn Stiles , Arthur LaMarche , Thomas Jelinek
发明人： Dominic Eisinger , Lynn Stiles , Arthur LaMarche , Thomas Jelinek
IPC分类号： G01N3353
CPC分类号： C07K16/18 , C07K16/065
摘要： A purified or highly pure recombinant monoclonal antibody with 4G10-hybridoma type specificity is disclosed along with polynucleotides, including cDNA sequences, encoding the antibody chains and the amino acid sequences corresponding to said cDNA polynucleotides and uses for said sequences. Also disclosed are corresponding tagged sequences useful in molecular biological techniques and uses for said sequences.
摘要翻译：公开了具有4G10-杂交瘤型特异性的纯化的或高纯度的重组单克隆抗体连同多核苷酸，包括编码抗体链的cDNA序列和对应于所述cDNA多核苷酸的氨基酸序列和所述序列的用途。还公开了在分子生物学技术中有用的对应的标记序列和所述序列的用途。

6. 发明授权

US06818407B2 Anti-endotoxic antimicrobial cationic peptides and methods of use therfor 失效
标题翻译：抗内毒素抗菌阳离子肽及其使用方法
公开(公告)号：US06818407B2
公开(公告)日：2004-11-16
申请号：US09908139
申请日：2001-07-17
申请人： Robert E. W. Hancock , Monisha A. Gough , Aleksander Patrzykat , Donald Woods , Xiaoyan Jia
发明人： Robert E. W. Hancock , Monisha A. Gough , Aleksander Patrzykat , Donald Woods , Xiaoyan Jia
IPC分类号： G01N3353
CPC分类号： A61K31/711 , A61K38/00 , C07K14/4723 , Y02A50/473 , Y02A50/481
摘要： A novel class of cationic peptides having antimicrobial activity is provided. Exemplary peptides of the invention include KWKSFIKKLTSAAKKVVTTAKPLALIS (SEQ ID NO:3) and KGWGSFFKKAAHVGKHVGKAALTHYL (SEQ ID NO:15). Also provided are methods for inhibiting the growth of bacteria utilizing the peptides of the invention. Such methods are useful for the treatment of respiratory infections, such as in cystic fibrosis patients. Such methods are further useful for accelerating wound healing.
摘要翻译：提供了一类具有抗菌活性的新型阳离子肽。本发明的示例性肽包括KWKSFIKKLTSAAKKVVTTAKPLALIS（SEQ ID NO：3）和KGWGSFFKKAAHVGKHVGKAALTHYL（SEQ ID NO：15）。还提供了利用本发明的肽抑制细菌生长的方法。这些方法可用于治疗呼吸道感染，如囊性纤维化患者。这种方法对于加速伤口愈合更有用。

7. 发明授权

US06806058B2 Secretions of proteins by encapsulated cells 失效
标题翻译：通过封装细胞分泌蛋白质
公开(公告)号：US06806058B2
公开(公告)日：2004-10-19
申请号：US10157351
申请日：2002-05-28
申请人： Diana Jesperson , Yevgenya Akselband , Patricia McGrath , Jan Trnovsky , Phillip T. Moen
发明人： Diana Jesperson , Yevgenya Akselband , Patricia McGrath , Jan Trnovsky , Phillip T. Moen
IPC分类号： G01N3353
CPC分类号： G01N33/5432 , C07K16/00 , C07K2317/52 , G01N33/5436 , G01N33/56966 , G01N33/56972 , Y10S436/823
摘要： The invention provides methods of analzying a secreted protein from a cell encapsulated in a microdrop. The microdrop is formulated with biotinylated matrix molecules at a reduced ratio of biotin to matrix molecules compared with previous formulations. The reduced ratio is advantageous for improving the resolution of detection and allows simultaneous detection of multiple secreted proteins and/or multiple cell surface markers. The invention further provides inter alia methods of isolating IgG isotype antibodies that have switched from IgM isotype.
摘要翻译：本发明提供了从包封在微滴中的细胞中分泌分泌蛋白质的方法。与以前的制剂相比，微滴与生物素基质分子配制，生物素与基质分子的比例降低。减少的比例对于改善检测的分辨率是有利的，并且允许同时检测多种分泌蛋白质和/或多种细胞表面标志物。本发明进一步提供了分离从IgM同种型转换的IgG同种型抗体的方法。

8. 发明授权

US06806055B2 HIV envelopolype peptides 失效
标题翻译： HIV包膜蛋白肽
公开(公告)号：US06806055B2
公开(公告)日：2004-10-19
申请号：US09966931
申请日：2001-09-27
申请人： Phillip W. Berman , Gerald R. Nakamura
发明人： Phillip W. Berman , Gerald R. Nakamura
IPC分类号： G01N3353
CPC分类号： C07K14/005 , A61K39/00 , C07K16/1063 , C07K2317/34 , C12N2740/16122
摘要： A method for the rational design and preparation of vaccines based on HIV envelope polypeptides is described. In one embodiment, the method for making an HIV gp120 subunit vaccine for a geographic region comprises determining neutralizing epitopes in the V2 and/or C4 domains of gp120 of HIV isolates from the geographic region and selecting an HIV strain having gp120 a neutralizing epitope in the V2 or C4 domain which is common among isolates in the geographic region. In a preferred embodiment of the method, neutralizing epitopes for the V2, V3, and C4 domains of gp120 are determined. At least two HIV isolates having different neutralizing epitopes in the V2, V3, or C4 domain are selected and used.to make the vaccine. The invention also provides a multivalent HIV gp120 subunit vaccine. A DNA sequence encoding gp120 from preferred vaccine strains of HIV, GNE8 and GNE16, expression constructs comprising the GNE8-gp120 and GNE16-gp120 encoding DNA under the transcriptional and translational control of a heterologous promoter, and isolated GNE8-gp120 and GNE16-gp120 are also described.
摘要翻译：描述了基于HIV包膜多肽合理设计和制备疫苗的方法。在一个实施方案中，用于制备用于地理区域的HIV gp120亚单位疫苗的方法包括测定来自地理区域的HIV分离物的gp120的V2和/或C4结构域中的中和表位，并且选择具有gp120的HIV株， V2或C4结构域，这在地理区域中是分离的。在该方法的优选实施方案中，测定gp120的V2，V3和C4结构域的中和表位。选择并使用至少两个在V2，V3或C4结构域中具有不同中和表位的HIV分离物来制备疫苗。本发明还提供了一种多价HIV gp120亚单位疫苗。编码来自HIV，GNE8和GNE16的优选疫苗株的gp120的DNA序列，在异源启动子和分离的GNE8-gp120和GNE16-gp120的转录和翻译控制下，包含编码DNA的GNE8-gp120和GNE16-gp120的表达构建体是也描述。

9. 发明授权

US06806054B2 Non-endogenous, constitutively activated known G protein-coupled receptors 失效
标题翻译：非内源性，组成型激活的已知G蛋白偶联受体
公开(公告)号：US06806054B2
公开(公告)日：2004-10-19
申请号：US09826509
申请日：2001-04-05
申请人： Karin Lehmann-Bruinsma , Chen W. Liaw , I-Lin Lin
发明人： Karin Lehmann-Bruinsma , Chen W. Liaw , I-Lin Lin
IPC分类号： G01N3353
CPC分类号： G01N33/74 , A61K38/00 , C07K14/705 , C07K14/723 , C07K2319/00 , G01N2333/726 , G01N2500/10
摘要： The invention disclosed in this patent document relates to transmembrane receptors, more particularly to a human G protein-coupled receptor for which the endogenous ligand is known (“known GPCRs”), and most particularly to mutated (non-endogenous) versions of the known GPCRs for use, most preferably in screening assays for the direct identification of candidate compounds as inverse agonists, agonists and partial agonists.
摘要翻译：本专利文献中公开的发明涉及跨膜受体，更具体地涉及内源性配体已知的人G蛋白偶联受体（“已知的GPCR”），最特别地涉及已知的突变的（非内源的）版本 GPCRs，最优选用于直接鉴定作为反向激动剂，激动剂和部分激动剂的候选化合物的筛选试验。

10. 发明授权

US06800447B2 Methods for identifying compounds which bind the active CCR5 chemokine receptor 有权
标题翻译：鉴定结合活性CCR5趋化因子受体的化合物的方法
公开(公告)号：US06800447B2
公开(公告)日：2004-10-05
申请号：US09939226
申请日：2001-08-24
申请人： Michel Samson , Marc Parmentier , Gilbert Vassart , Frederick Libert
发明人： Michel Samson , Marc Parmentier , Gilbert Vassart , Frederick Libert
IPC分类号： G01N3353
CPC分类号： C07K16/2866 , A01K2217/05 , A61K38/00 , A61K48/00 , C07K14/7158
摘要： A peptide has an amino acid sequence having more than 80% homology with the amino acid sequence listed as SEQ ID NO:4. A nucleic acid molecule has more than 80% homology with one of the nucleic sequences listed as SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3. Ligands, anti-ligands, cells vectors relating to the peptide and/or nucleic acid molecule are also used.
摘要翻译：肽具有与SEQ ID NO：4列出的氨基酸序列具有超过80％同源性的氨基酸序列。核酸分子与SEQ ID NO：1，SEQ ID NO：2和SEQ ID NO：3所列的核酸序列之一具有超过80％的同源性。还使用与肽和/或核酸分子相关的配体，抗配体，细胞载体。

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