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1. 发明申请

WO2019126479A1 PESTICIDAL GENES AND METHODS OF USE 审中-公开
公开(公告)号：WO2019126479A1
公开(公告)日：2019-06-27
申请号：PCT/US2018/066766
申请日：2018-12-20
申请人： AGBIOME, INC.
发明人： ROBERTS, Kira Bulazel , THAYER, Rebecca E. , PARKS, Jessica
IPC分类号： C12N15/82 , C12N15/31 , C12N15/32 , A01N63/02 , C07K14/32 , C07K14/325
CPC分类号： C07K14/325 , A01N63/02 , C07K14/32 , C12N15/8285 , C12N15/8286
摘要： Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptide sequences having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the pesticidal polypeptides, DNA constructs comprising the nucleic acid molecules, vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the pesticidal polypeptides. Nucleotide sequences encoding the polypeptides provided herein can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided herein are useful for the production of organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Methods are provided for producing the polypeptides disclosed herein, and for using those polypeptides for controlling a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included.

2. 发明申请

WO2017125583A1 SOUCHE BACTERIENNE GENETIQUEMENT MODIFIEE PRODUISANT DE LA KURSTAKINE DANS LE MILIEU DE CULTURE 审中-公开
标题翻译：遗传修饰的细菌菌株在培养环境中生成库尔斯克林
公开(公告)号：WO2017125583A1
公开(公告)日：2017-07-27
申请号：PCT/EP2017/051244
申请日：2017-01-20
申请人： INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE , UNIVERSITE DES SCIENCES ET TECHNOLOGIES DE LILLE , INSTITUT DES SCIENCES ET DES INDUSTRIES DU VIVANT ET DE L'ENVIRONNEMENT
发明人： LERECLUS, Didier , JACQUES, Philippe , GOHAR, Michel , CHOLLET, Marlène , CARADEC, Thibault , GÉLIS-JEANVOINE, Sébastien
IPC分类号： C12N1/21 , C12N15/52 , C07K14/32 , A01N63/02 , C07K7/06
CPC分类号： C12N15/52 , A01N63/02 , C07K14/32
摘要： La présente invention concerne une souche bactérienne génétiquement modifiée, en particulier une souche de Bacillus thuringiensis , exprimant la kurstakine et n'exprimant pas de toxine Cry, un procédé de production de kurstakine en milieu liquide et l'utilisation de la kurstakine en tant qu'agent antifongique et biosurfactant.
摘要翻译：本发明涉及经遗传修饰的细菌菌株，特别是苏云金芽孢杆菌菌株，其表达库尔斯塔金并表达没有Cry毒素，一个程序在液体培养基中生产库尔斯坦金霉素，以及使用kurstakine作为抗真菌剂和生物表面活性剂。

3. 发明申请

WO2017112733A1 ENHANCED PROTEIN PRODUCTION AND METHODS THEREOF 审中-公开
标题翻译：增强的蛋白质生产及其方法
公开(公告)号：WO2017112733A1
公开(公告)日：2017-06-29
申请号：PCT/US2016/067936
申请日：2016-12-21
申请人： DANISCO US INC.
发明人： BONGIORNI, Cristina , NEEF, Jolanda , SCHMIDT, Brian F. , VAN KIMMENADE, Anita , VAN DIJL, Jan Maarten
IPC分类号： C07K14/32 , C12P1/04
CPC分类号： C07K14/32 , C12N9/54 , C12P21/02 , C12Y304/24
摘要： The present disclosure is generally related to modified Gram-positive bacterial cells producing increased amounts of one or more protein(s) of interest. Certain embodiments of the instant disclosure are therefore directed to modified Gram-positive bacterial cells expressing an increased amount of a POI relative to unmodified ( i.e. , parental) Gram-positive bacterial cells, wherein the modified ( i.e. , daughter) bacterial cells comprise a modification which increases ras P gene expression. In certain other embodiments, the disclosure pertains to methods of modifying bacterial cells such that the modified (daughter) cells produce an increased level of a protein of interest. In other embodiments, the disclosure pertains to a protein of interest produced by fermenting a modified bacterial cell of the instant disclosure. Certain other embodiments of the disclosure are directed to one or more proteinaceous compositions comprising one or more protein(s) of interest thus made.
摘要翻译：本公开内容通常涉及产生增加量的一种或多种感兴趣蛋白质的修饰的革兰氏阳性细菌细胞。因此，本公开的某些实施方案涉及相对于未修饰（即，亲本）革兰氏阳性细菌细胞表达增加量的POI的修饰的革兰氏阳性细菌细胞，其中修饰的（

4. 发明申请

WO2017112538A2 PESTICIDAL GENES AND METHODS OF USE 审中-公开
标题翻译：杀虫基因和使用方法
公开(公告)号：WO2017112538A2
公开(公告)日：2017-06-29
申请号：PCT/US2016/067146
申请日：2016-12-16
申请人： AGBIOME, INC.
发明人： PARKS, Jessica , ROBERTS, Kira, Bulazel , THAYER, Rebecca, E.
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , A01N37/46 , A01N63/02 , C07K14/195 , C07K14/32 , C07K14/325 , C12N15/8261 , C12N15/8285 , Y02A40/146 , Y02A40/162 , Y02A40/164
摘要： Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptides having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the polypeptides, DNA constructs and vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the polypeptides. Polynucleotide sequences encoding the polypeptides can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided are useful for producing organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Such plants are resistant to insects and other pests. Methods are provided for producing the various polypeptides disclosed herein, and for using those polypeptides for controlling or killing a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included.
摘要翻译：提供了具有杀虫活性的组合物及其使用方法。组合物包括具有杀虫活性的分离和重组多肽，编码所述多肽的重组和合成核酸分子，包含所述核酸分子的DNA构建体和载体，包含所述载体的宿主细胞和所述多肽的抗体。编码多肽的多核苷酸序列可用于DNA构建体或表达盒中用于在感兴趣的生物体中转化和表达。提供的组合物和方法可用于生产具有增强的害虫抗性或耐受性的生物体。还提供了包含编码本发明杀虫蛋白的核苷酸序列的转基因植物和种子。这种植物对昆虫和其他害虫具有抗性。提供了用于产生本文公开的各种多肽的方法，以及使用这些多肽来控制或杀死害虫的方法。还包括用于检测样品中本发明多肽的方法和试剂盒。

5. 发明申请

WO2017070632A2 NUCLEOBASE EDITORS AND USES THEREOF 审中-公开
标题翻译：核编辑器及其用途
公开(公告)号：WO2017070632A2
公开(公告)日：2017-04-27
申请号：PCT/US2016/058344
申请日：2016-10-22
申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE
发明人： LIU, David, R. , KOMOR, Alexis Christine , REES, Holly A. , KIM, Yongjoo
IPC分类号： C07K14/32
CPC分类号： C12N9/22 , C07K14/32 , C07K2319/00 , C07K2319/09 , C07K2319/80 , C12N9/2497 , C12N9/78 , C12N15/907 , C12Y305/04005
摘要： Some aspects of this disclosure provide strategies, systems, reagents, methods, and kits that are useful for the targeted editing of nucleic acids, including editing a single site within the genome of a cell or subject, e.g., within the human genome. In some embodiments, fusion proteins of Cas9 and nucleic acid editing proteins or protein domains, e.g., deaminase domains, are provided. In some embodiments, methods for targeted nucleic acid editing are provided. In some embodiments, reagents and kits for the generation of targeted nucleic acid editing proteins, e.g., fusion proteins of Cas9 and nucleic acid editing proteins or domains, are provided.
摘要翻译：本公开的一些方面提供可用于核酸的靶向编辑的策略，系统，试剂，方法和试剂盒，包括编辑细胞或对象的基因组内的单个位点，例如，在人类基因组内。在一些实施方案中，提供了Cas9的融合蛋白和核酸编辑蛋白或蛋白结构域，例如脱氨酶结构域。在一些实施方案中，提供了用于靶向核酸编辑的方法。在一些实施方案中，提供用于产生靶向核酸编辑蛋白质的试剂和试剂盒，例如Cas9的融合蛋白和核酸编辑蛋白质或结构域。

6. 发明申请

WO2016180928A1 BACILLUS LICHENIFORMIS HOST CELL WITH DELETED LANTIBIOTIC GENE(S) 审中-公开
标题翻译： BACILLUS LICHENIFORMIS HOST CELL WITH DELETED LANTIBIOTIC GENE（S）
公开(公告)号：WO2016180928A1
公开(公告)日：2016-11-17
申请号：PCT/EP2016/060713
申请日：2016-05-12
申请人： NOVOZYMES A/S
发明人： KOEBMANN, Brian , RASMUSSEN, Michael, Dolberg
IPC分类号： C12N1/20 , C12P21/00 , C12N15/75
CPC分类号： C12N15/75 , C07K14/32 , C12P21/02
摘要： The present invention relates to Bacillus licheniformis host cells producing heterologous polypeptide of interest, wherein at least one gene in the Ian gene cluster inactivated and methods for producing the polypeptide of interest by cultivating said cells.
摘要翻译：本发明涉及产生感兴趣的异源多肽的地衣芽孢杆菌宿主细胞，其中Ian基因簇中的至少一个基因被灭活，并且通过培养所述细胞来产生感兴趣的多肽的方法。

7. 发明申请

WO2016100128A1 ENHANCED PROTEIN EXPRESSION 审中-公开
标题翻译：增强蛋白表达
公开(公告)号：WO2016100128A1
公开(公告)日：2016-06-23
申请号：PCT/US2015/065296
申请日：2015-12-11
申请人： DANISCO US INC
发明人： BONGIORNI, Cristina , CHRISTENSEN, Robert, I. , SCHMIDT, Brian, F. , VAN KIMMENADE, Anita
IPC分类号： C12N15/75 , C07K14/32
CPC分类号： C07K14/32 , C12N9/12 , C12N9/54 , C12N9/88 , C12N15/75 , C12P21/02 , C12R1/125
摘要： The present invention relates in general to bacterial cells having a genetic alteration that results in increased expression of a protein of interest and methods of making and using such cells. Aspects of the present invention include Grampositive microorganisms, such as members of the Bacillus genus having a genetic alteration that delays, reduces, or blocks the expression or activation of genes for sporulation, thereby resulting in enhanced expression of a protein of interest. The genetic alteration is one that reduces expression of a kin A gene, a phr A gene or a phr E gene.
摘要翻译：本发明一般涉及具有导致目的蛋白质表达增加的遗传改变的细菌细胞和制备和使用这些细胞的方法。本发明的方面包括Grampositive微生物，例如遗传改变的芽孢杆菌的成员，其延迟，减少或阻断用于孢子形成的基因的表达或活化，从而导致目的蛋白质的表达增强。遗传改变是减少kinA基因，phrA基因或phrE基因表达的改变。

8. 发明申请

WO2016050680A1 YOQM-INACTIVATION IN BACILLUS 审中-公开
标题翻译：豆蔻中的YOQM灭活
公开(公告)号：WO2016050680A1
公开(公告)日：2016-04-07
申请号：PCT/EP2015/072230
申请日：2015-09-28
申请人： NOVOZYMES A/S
发明人： BREÜNER, Anne , OESTERGAARD, Peter, Rahbek
IPC分类号： C07K14/32
CPC分类号： C07K14/32
摘要： The instant invention relates to mutant Bacillus host cells which comprise an inactivation of a polynucleotide encoding a YoqM polypeptide capable of binding to an affinity resin comprising bound bivalent nickel or cobalt ions; preferably sepharose or agarose functionalised with iminodiacetic acid (Ni-IDA) or nitrilotriacetic acid (Ni-NTA) for nickel and carboxyl- methylaspartate (Co-CMA) for cobalt; as well as methods of producing a polyhistidine-tagged polypeptide of interest in such mutant host cells.
摘要翻译：本发明涉及突变型芽孢杆菌宿主细胞，其包含编码能够结合包含结合的二价镍或钴离子的亲和树脂的YoqM多肽的多核苷酸的失活; 优选用用于钴的镍和羧基甲基天冬氨酸（Co-CMA）用亚氨基二乙酸（Ni-IDA）或次氮基三乙酸（Ni-NTA）官能化的琼脂糖或琼脂糖; 以及在这种突变宿主细胞中产生目标多聚组氨酸标记的多肽的方法。

9. 发明申请

WO2016048246A1 TARGETING OF MELANOCYTES FOR DELIVERING THERAPEUTIC OR DIAGNOSTIC AGENTS USING PROTEIN NANOCAGES 审中-公开
标题翻译：用于使用蛋白质纳米粒子递送治疗或诊断药物的MELANOCYTES的目标
公开(公告)号：WO2016048246A1
公开(公告)日：2016-03-31
申请号：PCT/SG2015/050343
申请日：2015-09-25
申请人： NANYANG TECHNOLOGICAL UNIVERSITY , NATIONAL SKIN CENTER (S) PTE LTD
发明人： LIM, Sierin , BHASKAR, Sathyamoorthy , THNG, Steven , KUNIYIL, Ambili , MRIDUL, Sarker
IPC分类号： C07K19/00 , A61K47/42 , C07K14/32 , A61K38/34 , C07K14/68 , C07K14/695 , A61K39/395 , C07K17/06 , A61P35/00 , A61P43/00
CPC分类号： A61K38/34 , A61K9/0014 , A61K38/35 , C07K14/195 , C07K14/32 , C07K14/47 , C07K14/685 , C07K14/69 , C07K14/695 , C07K14/723
摘要： The present invention relates to protein nanocages comprising a melanocyte-targeting moiety and pharmaceutical compositions comprising the protein cages as well as methods for treating or diagnosing hyperpigmentation disorders or other melanocyte-related disorders using the protein nanocages or pharmaceutical compositions. In the preferred embodiment, the protein nanocage is composed of Bacillus stearothermophilus E2 protein of pyruvate dehydrogenase multi-enzyme complex (E2), with a skin penetrating and cell permeating SPACE moiety, and a melanocyte-targeting moeity of alpha melanocyte stimulating hormone.
摘要翻译：本发明涉及包含黑素细胞靶向部分的蛋白质纳米笼和包含蛋白质笼的药物组合物以及使用蛋白质纳米笼或药物组合物治疗或诊断色素沉着过度障碍或其它黑素细胞相关病症的方法。在优选的实施方案中，蛋白质纳米笼菌由丙酮酸脱氢酶多酶复合物（E2）的嗜热脂肪芽孢杆菌E2蛋白质组成，具有皮肤穿透和细胞渗透SPACE部分，以及黑素细胞靶向的α黑素细胞刺激激素。

10. 发明申请

WO2016044655A2 FUSION PROTEINS, RECOMBINANT BACTERIA, AND METHODS FOR USING RECOMBINANT BACTERIA 审中-公开
标题翻译：融合蛋白，重组细菌和使用重组细菌的方法
公开(公告)号：WO2016044655A2
公开(公告)日：2016-03-24
申请号：PCT/US2015050795
申请日：2015-09-17
申请人： SPOGEN BIOTECH INC
发明人： THOMPSON BRIAN , SIEGEL ASHLEY
IPC分类号： C07K14/32
CPC分类号： C07K14/32 , A01N37/44 , A01N63/02 , A23K10/18 , A23K20/147 , A61K39/00 , A61L2/18 , C02F3/342 , C02F3/348 , C02F2101/10 , C02F2101/30 , C02F2103/002 , C02F2103/003 , C02F2103/007 , C02F2103/06 , C02F2103/10 , C02F2103/14 , C02F2103/16 , C02F2103/26 , C02F2103/28 , C07K2319/01 , C07K2319/035 , C07K2319/40 , C09K8/62 , C09K2208/24 , C12N1/20 , C12N3/00 , C12N15/62 , C12N15/75 , C12P21/02 , E21B43/16
摘要： Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifiations of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.
摘要翻译：提供了融合蛋白，其含有靶向序列，外孢壁蛋白或将融合蛋白靶向蜡状芽孢杆菌家族成员的外孢壁的外孢壁蛋白片段。还提供了表达这种融合蛋白的重组蜡状芽孢杆菌家族成员。还提供了基因失活的蜡状芽孢杆菌家族成员和过表达外孢菌蛋白的重组蜡状芽孢杆菌家族成员。还提供了用重组蜡状芽孢杆菌家族成员包被的种子和使用重组蜡状芽孢杆菌家族成员（例如，用于刺激植物生长）的方法。进一步提供了表达融合蛋白的重组蜡状芽孢杆菌家族成员的各种修饰。还提供了包含孢子外壳蛋白和感兴趣的蛋白质或肽的融合蛋白，表达此类融合蛋白的重组细菌，用此类重组细菌包被的种子，以及使用这种重组细菌的方法（例如用于刺激植物生长）。

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