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1. 发明申请

WO2019100018A1 METHODS FOR INCREASING THE RATE OF GENETIC PROGRESS AND GENERATING INBRED LINES IN NON-HUMAN MAMMALS USING GAMETES DERIVED FROM EMBRYOS 审中-公开
公开(公告)号：WO2019100018A1
公开(公告)日：2019-05-23
申请号：PCT/US2018/061850
申请日：2018-11-19
申请人： INGURAN, LLC
发明人： HEUER, Claas
IPC分类号： A01K67/00 , A01K67/02 , A01K67/027 , A61D19/00 , A61D19/04
CPC分类号： A01K67/027 , A01K2227/101 , A01K2267/02 , C12N15/8771 , C12Q1/6876 , C12Q2600/124
摘要： The invention encompasses methods of increasing the rate of genetic progress or generating inbred lines in a non-human mammalian species comprising the use of gametes derived from embryos of the non-human mammalian species.

2. 发明申请

WO2008017234A1 一种细胞核移植方法审中-公开
公开(公告)号：WO2008017234A1
公开(公告)日：2008-02-14
申请号：PCT/CN2007/002239
申请日：2007-07-23
申请人：上海交通大学附属儿童医院 , 上海滔滔转基因工程股份有限公司 , 曾溢滔 , 焦飞 , 曾凡一 , 黄淑帧
发明人：曾溢滔 , 焦飞 , 曾凡一 , 黄淑帧
IPC分类号： C12N5/06 , C12N5/16 , C12N15/87 , A01K67/027
CPC分类号： C12N15/8771
摘要： A cell nuclear transfer method, which conducted between the non-human mammal's nuclear donor somatic cell and the recipient oocyte possessing the same mitochondria DNA haplotype. The method can remarkably enhance the fusion rate, cleavage rate and blastula formation rate of the reconstructed embryos, therefore remarkably improves the cell nuclear transfer efficiency.

3. 发明申请

WO2004012499A2 A METHOD FOR THE RAPID SELECTION OF HOMOZYGOUS PRIMARY CELL LINES FOR THE PRODUCTION OF TRANSGENIC ANIMALS BY SOMATIC CELL NUCLEAR TRANSFER 审中-公开
标题翻译：用于通过自身细胞核转移快速选择用于生产转基因动物的原始细胞系的方法
公开(公告)号：WO2004012499A2
公开(公告)日：2004-02-12
申请号：PCT/US0321719
申请日：2003-07-14
申请人： GTC BIOTHERAPEUTICS INC , ECHELARD YANN , CHEN LI-HOW
发明人： ECHELARD YANN , CHEN LI-HOW
IPC分类号： A01K67/027 , C12N15/85 , C12N15/877 , A01K
CPC分类号： C12N15/8771 , A01K67/0273 , A01K2217/05 , A01K2227/10 , A01K2227/102 , A01K2267/01 , A01K2267/02 , C12N15/8509 , C12N15/877 , C12N15/8772
摘要： The present invention provides for the production of homozygous primary cells that carry a specific transgenic integration if interest on both chromosomes by bypassing breeding. These cell lines can they be used for the accelerated production of homozygous transgenic animals by somatic cell nuclear transfer. The invention is thus useful in the production of transgenic ungulate animals capable of producing desired biopharmaceuticals in their milk at higher yield than a comparable heterzygote. By combining the selection techniques of the current invention with somatic cell nuclear transfer it can be applied to large animals, where there is a strong need to shorten the time to homozygosity.
摘要翻译：本发明提供了通过绕过育种在两条染色体上感兴趣的情况下，产生携带特异性转基因整合的纯合原代细胞。这些细胞系可以用于通过体细胞核转移加速纯合子转基因动物的产生。因此，本发明可用于生产能够以比同类杂合子更高的产量在其乳中产生所需生物药物的转基因有蹄类动物。通过将本发明的选择技术与体细胞核转移相结合，可以应用于大型动物，其中强烈需要缩短纯合子的时间。

4. 发明申请

WO02077175A3 METHOD FOR GENERATING CLONED ANIMALS USING CHROMOSOME SHUFFLING 审中-公开
标题翻译：使用染色体标记生成克隆动物的方法
公开(公告)号：WO02077175A3
公开(公告)日：2002-12-05
申请号：PCT/US0208842
申请日：2002-03-25
申请人： ADVANCED CELL TECH INC , WEST MICHAEL D , CIBELLI JOSE
发明人： WEST MICHAEL D , CIBELLI JOSE
IPC分类号： A01K67/027 , C12N15/873 , C12N15/877 , G01N33/00 , C12N15/00
CPC分类号： A01K67/0275 , A01K2217/05 , A01K2227/101 , C12N15/873 , C12N15/877 , C12N15/8771 , C12N2517/00 , C12N2517/04
摘要： The present invention concerns the use of chromosomal replacement techniques in the context of producing cloned and transgenic animals, in order to correct chromosome abnormalities or alter autosomal genotypes, and provide for novel breeding pairs by replacing the sex chromosome in animals to be cloned. Replacement of a sex chromosome, or an X or Y chromosome, will result in animals that are autosomally isogenic and sexually non-isogenic (AISN), with ''autosomally isogenic'' meaning that the paired sets of autosomes (non-sex chromosomes) in each animal are isogenic or identical. Also included in the invention are animals that are both ''autosomally'' and ''allelically'' isogenic whereby each particular pair of chromosomes is internally isogenic or identical within a single animal as well as between animals. Such animals are particularly useful in generating a line of cloned mammals using sexual reproduction, without having to undergo nuclear transfer in order to propagate cloned animals.
摘要翻译：本发明涉及在产生克隆和转基因动物的情况下使用染色体替代技术，以校正染色体异常或改变常染色体基因型，并通过替代待克隆的动物中的性染色体来提供新的育种配对。替代性染色体或X或Y染色体将导致常染色体同源和性非同基因（AISN）动物，其中“常染色体同基因”意味着成对的常染色体组（非性染色体）在每只动物中是同基因的或相同的。本发明还包括“常染色体”和“等位基因”同源的动物，其中每对特定的染色体在单个动物内以及动物之间在内部是同基因的或相同的。这种动物在利用有性生殖产生一系列克隆哺乳动物时特别有用，而不必为了繁殖克隆动物而进行核转移。

5. 发明申请

WO2002077637A1 SEX-SPECIFIC SELECTION OF SPERM FROM TRANSGENIC ANIMALS 审中-公开
标题翻译：从转基因动物的特异性选择
公开(公告)号：WO2002077637A1
公开(公告)日：2002-10-03
申请号：PCT/US2002/008933
申请日：2002-03-22
申请人： INFIGEN, INC. , FORSBERG, Erik, J. , EILERTSEN, Kenneth, J. , BISHOP, Michael, D. , ZHENG, Ying , LENO, Gregory, H.
发明人： FORSBERG, Erik, J. , EILERTSEN, Kenneth, J. , BISHOP, Michael, D. , ZHENG, Ying , LENO, Gregory, H.
IPC分类号： G01N33/00
CPC分类号： C12N5/0612 , A01K67/0273 , A01K67/0275 , A01K2217/05 , A01K2227/101 , A01K2227/102 , A01K2227/103 , A01K2227/108 , A01K2267/02 , C07K14/46 , C12N15/8509 , C12N15/86 , C12N15/873 , C12N15/8771 , C12N15/8778 , C12N2510/00 , C12N2517/04
摘要： The present invention relates to methods and materials for pre-selecting the sex of mammalian offspring. In particular, the materials and methods described herein permit the enrichment of X- or Y-chromosome-bearing sperm in semen by introducing a transgene into a sex chromosome under control of regulatory sequences that provide for expression of the transgene in a haploid-specific manner.
摘要翻译：本发明涉及用于预先选择哺乳动物后代的性别的方法和材料。特别地，本文描述的材料和方法允许通过在以单倍体特异性方式提供转基因表达的调控序列的控制下将转基因导入性染色体中来富集在精液中的含有X或Y-染色体的精子。

6. 发明申请

WO9905266A2 TRANS-SPECIES NUCLEAR TRANSFER 审中-公开
标题翻译：转移核子转移
公开(公告)号：WO9905266A2
公开(公告)日：1999-02-04
申请号：PCT/US9815387
申请日：1998-07-24
申请人： WISCONSIN ALUMNI RES FOUND , FIRST NEAL L , DOMINKO TANJA , MITALIPOUA MAISSAM
发明人： FIRST NEAL L , DOMINKO TANJA , MITALIPOUA MAISSAM
IPC分类号： C12N15/877 , C12N15/00 , A01K67/027
CPC分类号： C12N15/8771
摘要： A method of producing cloned nuclear transfer embryos from differentiated donor cells is described. The method includes culturing differentiated donor cells in low serum medium and using bovine oocytes as recipient oocytes for donor cells from different species. This method may be used to produce genetically identical animals and transgenic animals.
摘要翻译：描述了从分化的供体细胞产生克隆的核转移胚胎的方法。该方法包括在低血清培养基中培养分化供体细胞，并使用牛卵母细胞作为来自不同物种的供体细胞的受体卵母细胞。该方法可用于产生遗传相同的动物和转基因动物。

7. 发明申请

WO99001164A1 CLONING PIGS USING DONOR NUCLEI FROM DIFFERENTIATED CELLS 审中-公开
标题翻译：克隆使用来自不同细胞的多核的核
公开(公告)号：WO99001164A1
公开(公告)日：1999-01-14
申请号：PCT/US1998/012806
申请日：1998-07-01
IPC分类号： A01K67/02 , A01K67/027 , A61K35/12 , A61K38/00 , A61K48/00 , A61P1/16 , A61P1/18 , A61P3/10 , A61P9/00 , A61P9/04 , A61P13/02 , A61P17/00 , A61P17/02 , A61P19/00 , A61P21/00 , A61P25/00 , A61P25/16 , A61P25/28 , A61P31/18 , A61P35/00 , C12N5/073 , C12N5/10 , C12N15/09 , C12N15/877 , A61K49/00 , C12N5/00 , C12N15/00
CPC分类号： C12N15/8778 , A01K67/027 , A01K67/0273 , A01K67/0275 , A01K2217/05 , A01K2227/101 , A61K35/12 , A61K38/00 , C12N5/0603 , C12N15/8771 , C12N2510/00 , C12N2517/02 , C12N2517/04
摘要： An improved method of nuclear transfer involving the transplantation of donor differentiated pig cell nuclei into enucleated pig oocytes is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring. Production of genetically engineered or transgenic pig embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.
摘要翻译：提供了涉及将供体分化的猪细胞核移植到去核猪卵母细胞中的改进的核转移方法。所产生的核转移单位可用于通过胎儿和后代的产生来增殖基因型和转基因基因型。通过本发明的方法促进转基因植物或转基因猪胚胎，胎儿和后代的生产，因为供体核的分化细胞源可被遗传修饰和克隆繁殖。

8. 发明申请

WO98030683A2 NUCLEAR TRANSFER WITH DIFFERENTIATED FETAL AND ADULT DONOR CELLS 审中-公开
标题翻译：具有差异化合物和成熟细胞的核转移
公开(公告)号：WO98030683A2
公开(公告)日：1998-07-16
申请号：PCT/US1998/000002
申请日：1998-01-05
IPC分类号： A01K67/02 , A01K67/027 , A61K35/12 , A61P1/18 , A61P3/10 , A61P9/00 , A61P9/04 , A61P13/02 , A61P17/02 , A61P21/00 , A61P25/16 , A61P25/28 , A61P31/18 , A61P35/00 , C12N5/073 , C12N5/10 , C12N15/09 , C12N15/877 , C12N15/00 , C12N5/06
CPC分类号： A01K67/0275 , A01K67/027 , A01K2217/05 , A01K2227/101 , A01K2227/106 , A61K35/12 , C12N5/0603 , C12N15/8771 , C12N15/8778 , C12N2510/00 , C12N2517/02 , C12N2517/04
摘要： An improved method of nuclear transfer involving the transplantation of donor differentiated cell nuclei into enucleated oocytes of the same species as the donor cell is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring, and for production of isogenic CICM cells, including human isogenic embryonic or stem cells. Production of genetically engineered or transgenic mammalian embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.
摘要翻译：提供了涉及将供体分化的细胞核移植到与供体细胞相同物种的去核卵母细胞中的改进的核转移方法。所产生的核转移单位可用于通过胎儿和后代的产生以及用于产生包括人同种异体胚胎或干细胞在内的等基因CICM细胞的基因型和转基因基因型的倍增。通过本方法促进遗传工程或转基因哺乳动物胚胎，胎儿和后代的生产，因为供体核的分化细胞源可以被遗传修饰和克隆繁殖。

9. 发明申请

WO1996007732A1 TOTIPOTENT CELLS FOR NUCLEAR TRANSFER 审中-公开
标题翻译：用于核转移的细胞细胞
公开(公告)号：WO1996007732A1
公开(公告)日：1996-03-14
申请号：PCT/GB1995002095
申请日：1995-09-05
申请人： ROSLIN INSTITUTE (EDINBURGH) , McWHIR, James , CAMPBELL, Keith, Henry, Stockman
发明人： ROSLIN INSTITUTE (EDINBURGH)
IPC分类号： C12N05/06
CPC分类号： C12N15/8771 , C12N5/0603 , C12N2517/10
摘要： Animal cells which are totipotent for nuclear transfer (TNT cells) can be isolated from the embryonic disc of the blastodermic vesicle of an ungulate, or the equivalent tissue of an embryo at an equivalent stage in non-ungulate species. TNT cells can be cultured in vitro, which allows cloning as desired and genetic manipulation, for example to introduce a transgene. Their nuclei can be transferred to suitable recipient cells and embryos reconstituted. In this way, animals of high genetic merit may be cloned and transgenic animals may be generated by mass transformation techniques across a broader range of species than is accessible with embryonic stem cell technology and without reliance on pronuclear microinjection.
摘要翻译：可用于核转移（TNT细胞）的全能动物细胞可以从有蹄类动物的胚胎性小泡的胚胎盘或非同型物种等效阶段的胚胎的等效组织中分离出来。可以在体外培养TNT细胞，其允许根据需要进行克隆和遗传操作，例如引入转基因。它们的核可以转移到合适的受体细胞中并重建胚胎。以这种方式，可以克隆高遗传优点的动物，并且可以通过跨越更广范围物种的大规模转化技术产生转基因动物，而不是通过胚胎干细胞技术可获得的，而不依赖于原核显微注射。

10. 发明申请

WO1992006215A1 Y-CHROMOSOME SPECIFIC POLYNUCLEOTIDE PROBES FOR PRENATAL SEXING 审中-公开
标题翻译： Y染色体特异性多核苷酸探针
公开(公告)号：WO1992006215A1
公开(公告)日：1992-04-16
申请号：PCT/CA1991000353
申请日：1991-10-03
申请人： THE UNIVERSITY OF CALGARY , WINKFEIN, Robert, J. , DIXON, Gordon, H.
发明人： THE UNIVERSITY OF CALGARY
IPC分类号： C12Q01/68
CPC分类号： C12N15/8771 , C12Q1/6841 , C12Q1/6879
摘要： Y-chromosome specific hybridization probes for prenatal sexing are provided capable of hybridizing only to Y-chromosome specific DNA sequences of bovine and other ruminants and are suitable for sexing embryos at or before the time of embryo transfer with essentially 100 % accuracy.
摘要翻译：提供用于产前性别的Y染色体特异性杂交探针能够仅与牛和其他反刍动物的Y染色体特异性DNA序列杂交，并且适合于在胚胎移植时或之前以基本上100％的准确度对胚胎进行性别化。

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