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1. 发明申请

WO03070894A2 A NON-INVASIVE DIAGNOSTIC TEST UTILIZING HISTONE MODIFICATION MARKERS 审中-公开
标题翻译：使用组织修饰标记的非侵入性诊断测试
公开(公告)号：WO03070894A2
公开(公告)日：2003-08-28
申请号：PCT/US0304661
申请日：2003-02-19
申请人： UNIV VIRGINIA , CHROMA THERAPEUTICS LTD , ALLIS C DAVID , BRUNS DAVID E , DRUMMOND ALAN H
发明人： ALLIS C DAVID , BRUNS DAVID E , DRUMMOND ALAN H
IPC分类号： C07K1/22 , C07K16/18 , C12M1/00 , C12N15/09 , C12P19/34 , C12Q1/68 , G01N33/53 , G01N33/68 , G01N37/00 , C12N
CPC分类号： G01N33/6842 , C12Q1/6809 , G01N33/5308 , G01N33/68 , G01N33/6875 , C12Q2565/113 , C12Q2563/119
摘要： The present invention relates to the use of antibodies directed against specific histone amino terminus modifications as diagnostic indicators of disease or congenital defects. In one embodiment, nucleosomes are isolated from a blood or serum sample of a patient using histone specific antibodies and the accompanying DNA is purified and analyzed for diagnostic and screening purposes.
摘要翻译：本发明涉及针对特定组蛋白氨基末端修饰的抗体作为疾病或先天缺陷诊断指标的用途。在一个实施方案中，使用组蛋白特异性抗体从患者的血液或血清样品中分离核小体，并纯化并伴随DNA进行诊断和筛选。

2. 发明申请

WO00032812A1 METHOD FOR SEPARATING NUCLEIC ACIDS INTO POPULATIONS 审中-公开
标题翻译：将核酸分解成人口的方法
公开(公告)号：WO00032812A1
公开(公告)日：2000-06-08
申请号：PCT/GB1999/003995
申请日：1999-11-30
IPC分类号： G01N33/53 , C12N15/09 , C12N15/10 , C12Q20060101 , C12Q1/68 , G01N33/566
CPC分类号： C12N15/1006 , C12Q1/68 , C12Q1/6806 , C12Q1/6858 , C12Q1/686 , C12Q2563/131 , C12Q2525/131 , C12Q2522/101 , C12Q2563/119
摘要： A method for at least partially separating nucleic acid molecules in a sample into populations wherein a population is tagged or capable of being tagged with a moiety capable of being immobilised on a matrix, said method comprising contacting the nucleic acid containing sample with a matrix whereby the tagged molecules are captured by the matrix and thereby separated from untagged molecules. The method may be used in a variety of applications, including separating restriction enzyme digestion products, separating linear from circular nucleic acid molecules, in vitro packaging of bacteriophage particles, diagnostic PCR.
摘要翻译：一种用于将样品中的核酸分子至少部分分离成群体的方法，其中群体被标记或能够被能够被固定在基质上的部分标记，所述方法包括使含有核酸的样品与基质接触，由此，标记的分子被基质捕获，从而与未标记的分子分离。该方法可用于各种应用，包括分离限制酶消化产物，从环状核酸分子线性分离，噬菌体颗粒的体外包装，诊断性PCR。

3. 发明申请

WO99006591A1 METHODS FOR DETECTING MUTATION IN BASE SEQUENCE 审中-公开
标题翻译：用于检测基本序列中的突变的方法
公开(公告)号：WO99006591A1
公开(公告)日：1999-02-11
申请号：PCT/JP1998/003413
申请日：1998-07-30
IPC分类号： C12Q1/68 , C12M1/32 , C12N15/11 , G01N33/58
CPC分类号： C12Q1/6827 , C12Q2565/501 , C12Q2563/131 , C12Q2521/514 , C12Q2563/119 , C12Q2525/107
摘要： A method for detecting a mutated nucleic acid and/or DNA fragment which comprises hybridizing a nucleic acid fragment, etc. immobilized on a plate with another nucleic acid fragment, etc. to be examined in the presence of the mutation, binding the thus formed inappropriate base pair to a substance capable of binding specifically to the inappropriate base pair, such as labeled Mut S, and then detecting and identifying the fragment to which the above substance has been bound; a method which comprises treating, in place of the above substance capable of binding specifically to the inappropriate pair, an inappropriate base pair formed between hybridized fragments with a substance capable of specifically recognizing and cleaving the inappropriate base pair, thus cleaving or removing the hybridized fragment starting with the inappropriate base pair, labeling the fragment remaining on a plate after the cleavage or removal, and then detecting and identifying the thus labeled fragment; and substances capable of binding specifically to an inappropriate base pair such as GFP-labeled Mut S. Thus, structural mutations in two or more genes can be detected in parallel. In particular, structural mutations can be detected while monitoring the extent of expression simultaneously.
摘要翻译：一种用于检测突变的核酸和/或DNA片段的方法，其包括在所述突变的存在下将固定在板上的核酸片段等与待检测的另一核酸片段等杂交，从而结合如此形成的不适当碱基对能够特异性结合不适当碱基对的物质，例如标记的Mut S，然后检测和鉴定与上述物质结合的片段; 一种方法，其包括将能够特异性结合不适当对的上述物质替换为能够特异性识别和切割不适当碱基对的物质在杂交片段之间形成的不适当碱基对，从而切割或除去杂交片段以不适当的碱基对开始，在切割或去除后标记残留在平板上的片段，然后检测和鉴定如此标记的片段; 以及能够特异性结合不适当碱基对（如GFP标记的MutS）的物质。因此，可以并行检测两个或多个基因中的结构突变。特别地，可以同时监测表达的程度来检测结构突变。

4. 发明申请

WO1992010588A1 SEQUENCING BY HYBRIDIZATION OF A TARGET NUCLEIC ACID TO A MATRIX OF DEFINED OLIGONUCLEOTIDES 审中-公开
标题翻译：通过将目标核酸杂交到定义的寡核苷酸的矩阵进行测序
公开(公告)号：WO1992010588A1
公开(公告)日：1992-06-25
申请号：PCT/US1991009226
申请日：1991-12-06
申请人： AFFYMAX TECHNOLOGIES N.V. , FODOR, Stephen, P., A. , SOLAS, Dennis, W. , DOWER, William, J.
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C12Q01/68
CPC分类号： G03F7/00 , B01J19/0046 , B01J2219/00315 , B01J2219/00432 , B01J2219/00434 , B01J2219/00436 , B01J2219/00459 , B01J2219/00468 , B01J2219/00475 , B01J2219/005 , B01J2219/00527 , B01J2219/00529 , B01J2219/00531 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00648 , B01J2219/00659 , B01J2219/00689 , B01J2219/00695 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B82Y10/00 , B82Y30/00 , C07B2200/11 , C07H19/10 , C07H21/00 , C07K1/042 , C07K1/045 , C07K17/06 , C07K17/14 , C12Q1/6804 , C12Q1/6809 , C12Q1/6816 , C12Q1/6827 , C12Q1/6834 , C12Q1/6837 , C12Q1/6874 , C40B40/06 , C40B40/10 , C40B60/14 , G01N15/1475 , G03F7/265 , G03F7/38 , G11C13/0014 , G11C13/0019 , C12Q2565/513 , C12Q2565/507 , C12Q2545/101 , C12Q2525/185 , C12Q2565/514 , C12Q2563/173 , C12Q2563/119 , C12Q2563/149 , C12Q2525/151 , C12Q2525/179 , C12Q2525/204
摘要： The present invention provides methods and appartus for sequencing, fingerprinting and mapping biological polymers, particularly polynucleotides. The methods make use of a plurality of positionally distinct sequence specific recognition reagents, such as polynucleotides. The apparatus employs a substrate comprising positionally distinct sequence specific recognition reagents, such as polynucleotides, which are preferably localized at high densities. The methods and apparatus of the present invention can be used for determining the sequence of polynucleotides, mapping polynucleotides, and developing polynucleotide fingerprints. Polynucleotide fingerprints can be used for identifying individuals, tissue samples, pathological conditions, genetic diseases, infectious diseases, and other applications. Polynucleotide fingerprints can also be used for classification of bioligical samples, including taxonomy, and to characterize their sources. The invention also provides polynucleotide mapping, fingerprinting, and sequencing as valuable laboratory research tools for use in biological investigations.
摘要翻译：本发明提供了用于测序，指纹和映射生物聚合物，特别是多核苷酸的方法和应用。所述方法利用多种位置不同的序列特异性识别试剂，例如多核苷酸。该装置使用包含位置不同的序列特异性识别试剂（例如多核苷酸）的底物，其优选以高密度定位。本发明的方法和装置可以用于确定多核苷酸序列，映射多核苷酸和开发多核苷酸指纹。多核苷酸指纹可用于鉴定个体，组织样本，病理状况，遗传疾病，传染病和其他应用。多核苷酸指纹也可用于生物样本的分类，包括分类学，并对其来源进行表征。本发明还提供多核苷酸定位，指纹和测序作为用于生物学研究的有价值的实验室研究工具。

5. 发明申请

WO2015138405A2 DETECTION AND QUANTIFICATION OF METHYLATION IN DNA 审中-公开
标题翻译： DNA甲基化的检测和定量
公开(公告)号：WO2015138405A2
公开(公告)日：2015-09-17
申请号：PCT/US2015/019630
申请日：2015-03-10
申请人： THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS , MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH , BASHIR, Rashid , VENKATESAN, Bala Murali , VASMATZIS, George , SHIM, Jiwook
发明人： BASHIR, Rashid , VENKATESAN, Bala Murali , VASMATZIS, George , SHIM, Jiwook
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6825 , G01N33/48721 , C12Q2522/101 , C12Q2537/164 , C12Q2563/116 , C12Q2563/119 , C12Q2565/631
摘要： Provided are methods and systems for characterizing a biomolecular parameter of a polynucleotide. A polynucleotide of interest from a sample comprising a heterogeneous mixture of polynucleotides is concentrated and provided to a first fluid compartment of a solid-state nanopore. An electric potential is established across the solid-state nanopore to force the polynucleotide of interest from a first fluid compartment to a second fluid compartment via the nanopore. A passage parameter output is monitored during passage of the polynucleotide of interest through the nanopore, wherein the passage parameter output depends on the biomolecular parameter status of the polynucleotide of interest. In this manner, the methods and systems are compatible with a wide range of applications, including epigenetic modifications to DNA indicative of a disease state such as cancer, in an integrated, reliable and low cost system.
摘要翻译：提供了用于表征多核苷酸的生物分子参数的方法和系统。将来自包含多核苷酸的非均质混合物的样品的感兴趣的多核苷酸浓缩并提供给固态纳米孔的第一流体隔室。跨越固态纳米孔建立电势以经由纳米孔将感兴趣的多核苷酸从第一流体隔室强制到第二流体隔室。在目标多核苷酸通过纳米孔期间监测通道参数输出，其中通道参数输出取决于目的多核苷酸的生物分子参数状态。以这种方式，这些方法和系统与一系列广泛的应用相兼容，包括在一个集成的，可靠的和低成本的系统中对指示疾病状态的DNA如癌症的表观遗传修饰。

6. 发明申请

WO2013191265A1 タンパク質－核酸複合体の製造方法および標的物の検出方法审中-公开
标题翻译：生产蛋白质 - 核酸复合物的方法和检测目标物质的方法
公开(公告)号：WO2013191265A1
公开(公告)日：2013-12-27
申请号：PCT/JP2013/067018
申请日：2013-06-14
申请人：国立大学法人九州大学 , 日立アロカメディカル株式会社
发明人：神谷　典穂 , 林　浩之輔 , 永井　賢治
IPC分类号： C12P19/34 , C12Q1/68 , G01N33/53
CPC分类号： C12Q1/6804 , C12P19/34 , C12P21/02 , G01N33/5308 , G01N33/532 , G01N33/58 , G01N33/582 , C12Q2521/119 , C12Q2521/131 , C12Q2525/205 , C12Q2563/119
摘要：　良好な感度で標的物を検出可能な核酸プローブの製造方法を提供する。核酸の３'末端にヌクレオチドを付加する３'末端付加酵素を用いて、核酸の３'末端に、グルタミン（Ｇｌｎ）残基またはリシン（Ｌｙｓ）残基を有するヌクレオシド三リン酸誘導体を少なくとも１つ付加する３'末端付加工程と、トランスグルタミナーゼ（ＴＧａｓｅ）を用いて、前記グルタミン（Ｇｌｎ）残基にリシン（Ｌｙｓ）残基を有し標識部分を含む標識化合物を結合する、または、前記リシン（Ｌｙｓ）残基にグルタミン（Ｇｌｎ）残基を有し標識部分を含む標識化合物を結合する標識化合物結合工程と、を含む核酸プローブの製造方法である。
摘要翻译：本发明提供能够以良好的灵敏度检测目标物质的核酸探针的制造方法。一种生产核酸探针的方法，包括：3'-末端加成步骤，将至少一种具有谷氨酰胺（Gln）残基或赖氨酸（Lys）残基的核苷三磷酸衍生物加入核酸的3'末端使用可以向核酸的3'末端添加核苷酸的3'-末端加成酶; 以及使用转谷氨酰胺酶（TGase）将具有赖氨酸（Lys）残基并含有标记部分的标记化合物与谷氨酰胺（Gln）残基结合的标记化合物结合步骤或结合具有谷氨酰胺（Gln）残基的标记化合物并含有使用转谷氨酰胺酶（TGase）的赖氨酸（Lys）残基的标记部分。

7. 发明申请

WO2011040996A1 ULTRAFAST SEQUENCING OF BIOLOGICAL POLYMERS USING A LABELED NANOPORE 审中-公开
标题翻译：使用标记纳米粒子的生物聚合物的超快速测序
公开(公告)号：WO2011040996A1
公开(公告)日：2011-04-07
申请号：PCT/US2010/034809
申请日：2010-05-13
申请人： QUANTAPORE, INC. , HUBER, Martin
发明人： HUBER, Martin
IPC分类号： G01N33/00 , B82B3/00
CPC分类号： C12Q1/6869 , G01N21/6428 , G01N33/48721 , G01N2021/6432 , C12Q2563/155 , C12Q2563/119 , C12Q2563/107 , C12Q2565/531 , C12Q2565/101 , C12Q2561/113
摘要： Methods and systems for sequencing a biological molecule or polymer, e.g., a nucleic acid, are provided. One or more donor labels, which are attached to a pore or nanopore, may be illuminated or otherwise excited. A polymer having a monomer labeled with one or more acceptor labels, may be translocated through the pore. Either before, after or while the labeled monomer of the polymer passes through, exits or enters the pore, energy may be transferred from the excited donor label to the acceptor label of the monomer. As a result of the energy transfer, the acceptor label emits energy, and the emitted energy is detected in order to identify the labeled monomer of the translocated polymer and to thereby sequence the polymer.
摘要翻译：提供了用于测序生物分子或聚合物例如核酸的方法和系统。连接到孔或纳米孔上的一个或多个供体标记可以被照亮或以其它方式激发。具有由一个或多个受体标记物标记的单体的聚合物可以通过孔易位。在聚合物的标记单体通过之前，之后或之后，离开或进入孔，能量可以从激发的供体标记转移到单体的受体标记。作为能量转移的结果，受体标记发射能量，并且检测发射的能量，以便鉴定易位聚合物的标记单体，从而对聚合物进行顺序。

8. 发明申请

WO2005031305A3 SURFACE IMMOBILIZED POLYELECTROLYTE WITH MULTIPLE FUNCTIONAL GROUPS CAPABLE OF COVALENTLY BONDING TO BIOMOLECULES 审中-公开
标题翻译：具有多个功能组合的表面固定聚电解质，可共同连接生物分子
公开(公告)号：WO2005031305A3
公开(公告)日：2006-03-30
申请号：PCT/US2004031058
申请日：2004-09-22
申请人： BIOARRAY SOLUTIONS LTD , WANG XINWEN , BANERJEE SUKANTA
发明人： WANG XINWEN , BANERJEE SUKANTA
IPC分类号： C12M1/34 , C12Q1/68 , G01N20060101 , G01N33/53 , G01N33/543 , B05D3/00 , B32B9/04 , C07H21/04
CPC分类号： C12Q1/6834 , C07H21/04 , G01N33/54353 , C12Q2563/119
摘要： A polyelectrolyte having multiple exposed functional groups, each such group being capable of covalently bonding to a molecule, is immobilized on a surface for the purpose of bonding to a biomolecule. The biomolecule can be, for example, a nucleic acid, e.g., an amine functionalized oligonucleotide. The polyelectrolyte can include, e.g., BSA (Bovine Serum Albumin) which is bound to a functionalized surface using a covalent immobilization strategy, e.g., reaction with the surface of a tosyl-activated microparticle. Following such reaction, exposed reactive functional groups on the protein, such as amine, carboxyl, thiol, hydroxyl groups can further be utilized to covalently couple the oligonucleotide of interest using suitable chemistry.
摘要翻译：具有多个暴露的官能团的聚电解质，每个这样的基团能够共价键合到分子，固定在表面上用于与生物分子结合的目的。生物分子可以是例如核酸，例如胺官能化的寡核苷酸。聚电解质可以包括例如BSA（牛血清白蛋白），其使用共价固定策略结合到官能化表面，例如与甲苯磺酰基活化的微粒的表面反应。在这样的反应之后，蛋白质上暴露的反应性官能团如胺，羧基，硫醇，羟基可进一步用于使用合适的化学物质共价连接感兴趣的寡核苷酸。

9. 发明申请

WO99036575A1 MULTIPLEX VGID 审中-公开
公开(公告)号：WO99036575A1
公开(公告)日：1999-07-22
申请号：PCT/US1999/001037
申请日：1999-01-15
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/6827 , C12Q2563/119 , C12Q2527/143 , C12Q2522/10
摘要： The present invention relates generally to the field of genomics. More particularly, the present invention relates to a method for gene identification beginning with user-selected input phenotypes. The method is referred to generally as the V aliGene G ene I dentification method, or the VGID method. When more than two source populations of nucleic acids are simultaneously compared, the method may be referred to as multiplex VGID . The method employs nucleic acid mismatch binding protein chromatography to effect a molecular comparison of one phenotype with others. Genes are identified as having a specified function, or as causing or contributing to the cause or pathogenesis of a specified disease, or as associated with a specific phenotype, by virtue of their selection by the method. Identified genes may be used in development of reagents, drugs and/or combination thereof useful in clinical or other settings for prognosis, diagnosis and/or treatment of diseases, disorders and/or conditions. The method is equally suited for gene identification for agricultural, bio-engineering, medical, veterinary, and many other applications.
摘要翻译：本发明一般涉及基因组学领域。更具体地说，本发明涉及从用户选择的输入表型开始的用于基因鉴定的方法。该方法通常被称为认知方法或VGID 方法。当同时比较两个以上的核酸源群时，该方法可以称为多重VGID 。该方法采用核酸错配结合蛋白色谱法对其中一种表型进行分子比较。根据该方法的选择，将基因鉴定为具有指定功能，或引起或促成特定疾病的原因或发病机制，或与特定表型相关。鉴定的基因可用于开发用于临床或其它设置的用于疾病，病症和/或病症的预后，诊断和/或治疗的试剂，药物和/或其组合。该方法同样适用于农业，生物工程，医学，兽医和许多其他应用的基因鉴定。

10. 发明申请

WO2019078722A1 COACERVATE-BASED DETECTION OF A COMPOUND OF INTEREST 审中-公开
公开(公告)号：WO2019078722A1
公开(公告)日：2019-04-25
申请号：PCT/NL2018/050690
申请日：2018-10-19
申请人： TECHNISCHE UNIVERSITEIT DELFT
发明人： REESE, Louis , SPOELSTRA, Willem Kasper , BROUNS, Stan Johan Jozef
IPC分类号： C12Q1/6816
CPC分类号： C12Q1/6816 , C12Q2521/319 , C12Q2523/31 , C12Q2563/113 , C12Q2563/119 , C12Q2565/113
摘要： The present invention relates to the field of biotechnology, more specifically to the field of molecular diagnostics; to a method for coacervate-based detection of the presence of a compound of interest in a sample, wherein upon recognition of the compound of interest by the target recognition moiety, coacervate phase separation is either resolved or induced, wherein the extent of phase separation is a measure for the presence of the compound of interest.

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