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1. 发明申请

US20120193049A1 METHOD FOR PROCESSING LIGNOCELLULOSE MATERIAL 有权
标题翻译：加工纤维素材料的方法
公开(公告)号：US20120193049A1
公开(公告)日：2012-08-02
申请号：US13445894
申请日：2012-04-12
申请人： Xuefeng YU , Zhihong LI , Minghua YU , Juan YAO , Daiwu LIU , Jincheng LEI , Zhijun LI
发明人： Xuefeng YU , Zhihong LI , Minghua YU , Juan YAO , Daiwu LIU , Jincheng LEI , Zhijun LI
IPC分类号： D21C1/00
CPC分类号： C12P19/14 , C12P19/02 , Y02E50/16
摘要： A method for processing a lignocellulose material, comprises the following steps: (1) crushing and sieving the lignocellulose material, and collecting granules with a particle size of between 0.08 and 0.1 mm; (2) mixing the granules obtained in step (1) with water, and dispersing through a colloid mill to yield a suspension with a particle size of 40-80 μm; (3) homogenizing the suspension obtained in the step (2) under high pressure to have a particle size of between 10 and 40 μm; and (4) buffering the suspension obtained in the step (3) with a buffer solution of sodium acetate and acetic-acid, adding cellulase, β-glucosidase, and xylanase, and performing zymolysis for 36-72 hours.
摘要翻译：一种处理木质纤维素材料的方法，包括以下步骤：（1）对木质纤维素材料进行破碎和筛分，并收集粒度为0.08至0.1mm的颗粒; （2）将步骤（1）中获得的颗粒与水混合，并通过胶体磨分散，得到粒径为40-80μm的悬浮液; （3）在高压下使步骤（2）中得到的悬浮液均匀化，使其粒径在10〜40μm之间; 和（4）用乙酸钠和乙酸缓冲溶液缓冲步骤（3）中获得的悬浮液，加入纤维素酶，葡糖苷酶和木聚糖酶，并进行酶解36-72小时。

2. 发明授权

US08263374B2 Composite yeast suitable for high concentration alcohol fermentation from sugar-containing raw materials 有权
标题翻译：复合酵母适用于含糖原料的高浓度酒精发酵
公开(公告)号：US08263374B2
公开(公告)日：2012-09-11
申请号：US12668578
申请日：2008-07-30
申请人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Zhijun Li , Daiwu Liu
发明人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Zhijun Li , Daiwu Liu
IPC分类号： C12P7/14 , C12P7/06 , C12N1/00 , C12N1/12 , A01N63/04
CPC分类号： C12P39/00 , C12N1/16 , C12P7/14 , Y02E50/17
摘要： The present invention relates to a composite yeast suitable for high concentration alcohol fermentation from sugar-containing raw materials, characterizing in that the composite yeast comprises any kind of dried yeast selected from Brewers yeast Saccharomyces cerevisiae Hansen of Saccharomyces cerevisiae, grape wine yeast Saccharomyces uvarum Beijerinek, and nutritious materials which are necessary for yeast growth, the nutritious materials include: the dried yeast 40-70 parts by weight, a nitrogen source 20-40 parts by weight, a phosphorous source 5-10 parts by weight, an other inorganic salt 2.5-5 parts by weight, a trace vitamin 1-2.5 parts by weight and a bacteriostatic 0.5-1.2 parts by weight. The present invention further relates to a method for preparation of the composite yeast suitable for high concentration alcohol fermentation from sugar-containing raw materials. Using the composite yeast of the present invention to proceed with sugar fermentation can increase fermentation alcoholicity, decrease residual sugar content, and allows the final alcoholicity of standard raw material, such as sucrose to attain to 14.5-15.5% v/v, and the amount of the residual reducing sugar in the fermentation mash is 0-0.1 wt %.
摘要翻译：本发明涉及适用于含糖原料的高浓度酒精发酵的复合酵母，其特征在于复合酵母包含任何种类的干酵母，其选自酿酒酵母酿酒酵母酿酒酵母Hansen，酿酒酵母酿酒酵母Beijerinek 和酵母生长所必需的营养物质，营养物质包括：干燥酵母40-70重量份，氮源20-40重量份，磷源5-10重量份，其它无机盐 2.5-5重量份，微量维生素1-2.5重量份，抑菌剂0.5-1.2重量份。本发明还涉及一种适用于含糖原料的高浓度酒精发酵的复合酵母的制备方法。使用本发明的复合酵母进行糖发酵可以提高发酵酒精度，降低残留糖含量，并允许标准原料如蔗糖的最终酒精度达到14.5-15.5％v / v，的发酵麦芽汁中的残留还原糖为0-0.1重量％。

3. 发明申请

US20110171710A1 METHOD FOR PRODUCING CELLULOSIC ETHANOL 审中-公开
标题翻译：生产纤维素乙醇的方法
公开(公告)号：US20110171710A1
公开(公告)日：2011-07-14
申请号：US13054851
申请日：2009-09-27
申请人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Zhijun Li , Daiwu Liu
发明人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Zhijun Li , Daiwu Liu
IPC分类号： C12P7/10
CPC分类号： C12P7/10 , C12P7/065 , Y02E50/16 , Y02E50/17
摘要： A method for producing cellulosic ethanol is disclosed, which comprises the following steps: adding the medium containing the source materials of cellulose and/or hemicellulose to fermentation reaction vessel; adding cellulase to fermentation reaction vessel, and inoculating Candida lusitaniae; running simultaneous saccharification fermentation (SSFs) with the combined action of cellulose and Candida lusitaniae, and obtaining cellulosic ethanol by separation. Candida lusitaniae used in SSFs in present invention has higher ethanol tolerance and a higher ethanol production rate, it can use cellulose and hemicellulose as source materials and effectively produce cellulosic ethanol with cellulase in SSFs.
摘要翻译：公开了一种生产纤维素乙醇的方法，包括以下步骤：向发酵反应容器中加入含有纤维素和/或半纤维素原料的培养基; 向发酵反应容器中加入纤维素酶，接种假丝酵母; 通过纤维素和假丝酵母（Candida lusitaniae）的联合作用同时进行糖化发酵（SSFs），并通过分离获得纤维素乙醇。本发明SSF中使用的假丝酵母具有较高的乙醇耐受性和较高的乙醇生产率，可以使用纤维素和半纤维素作为原料，并在SSF中有效地生产纤维素酶纤维素乙醇。

4. 发明申请

US20080187987A1 Composite Yeast Suitable For High Concentration Alcohol Fermentation 有权
标题翻译：复合酵母适用于高浓度酒精发酵
公开(公告)号：US20080187987A1
公开(公告)日：2008-08-07
申请号：US11914475
申请日：2005-12-30
申请人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Zhijun Li , Daiwu Liu
发明人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Zhijun Li , Daiwu Liu
IPC分类号： C12N1/00
CPC分类号： C12N1/18 , C12C12/006 , C12G1/0203 , C12J1/00 , C12N9/16 , C12N9/2402 , C12N9/2437 , C12N9/244 , C12N9/60 , C12P7/06 , C12Y302/01004 , C12Y302/01006 , C12Y302/01015 , Y02E50/17
摘要： The present invention relates to a composite yeast for high concentration alcohol fermentation, which includes thermostable Saccharomyces cerevisiae, acid protease, phytase, cellulose, β-glucanase, and pectinase, and is suitable for the high concentration alcohol fermentation for various raw materials. In addition to normal fermentation, the composite yeast of the present invention can degrade the raw materials, increase the nutrient ingredients in the mash, promote the growth of yeast and provide stress tolerance protection.
摘要翻译：本发明涉及一种用于高浓度酒精发酵的复合酵母，其包括耐热酿酒酵母，酸性蛋白酶，植酸酶，纤维素，β-葡聚糖酶和果胶酶，并且适用于各种原料的高浓度酒精发酵。除正常发酵外，本发明的复合酵母可以降解原料，增加醪液中的营养成分，促进酵母生长，提供耐应力保护。

5. 发明授权

US08828187B2 Method for processing lignocellulose material 有权
标题翻译：木质纤维素材料的处理方法
公开(公告)号：US08828187B2
公开(公告)日：2014-09-09
申请号：US13445894
申请日：2012-04-12
申请人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Daiwu Liu , Jincheng Lei , Zhijun Li
发明人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Daiwu Liu , Jincheng Lei , Zhijun Li
IPC分类号： D21C1/00 , C12P19/14
CPC分类号： C12P19/14 , C12P19/02 , Y02E50/16
摘要： A method for processing a lignocellulose material, comprises the following steps: (1) crushing and sieving the lignocellulose material, and collecting granules with a particle size of between 0.08 and 0.1 mm; (2) mixing the granules obtained in step (1) with water, and dispersing through a colloid mill to yield a suspension with a particle size of 40-80 μm; (3) homogenizing the suspension obtained in the step (2) under high pressure to have a particle size of between 10 and 40 μm; and (4) buffering the suspension obtained in the step (3) with a buffer solution of sodium acetate and acetic-acid, adding cellulase, β-glucosidase, and xylanase, and performing zymolysis for 36-72 hours.
摘要翻译：一种处理木质纤维素材料的方法，包括以下步骤：（1）对木质纤维素材料进行破碎筛分，并收集粒度在0.08至0.1mm之间的颗粒; （2）将步骤（1）中获得的颗粒与水混合，并通过胶体磨分散，得到粒径为40-80μm的悬浮液; （3）在高压下使步骤（2）中得到的悬浮液均匀化，使其粒径在10〜40μm之间; 和（4）用乙酸钠和乙酸缓冲溶液缓冲步骤（3）获得的悬浮液，加入纤维素酶，葡糖苷酶和木聚糖酶，并进行酶解36-72小时。

6. 发明授权

US08697425B2 Composite yeast suitable for high concentration alcohol fermentation 有权
标题翻译：复合酵母适用于高浓度酒精发酵
公开(公告)号：US08697425B2
公开(公告)日：2014-04-15
申请号：US11914475
申请日：2005-12-30
申请人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Zhijun Li , Daiwu Liu
发明人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Zhijun Li , Daiwu Liu
IPC分类号： C12N1/18
CPC分类号： C12N1/18 , C12C12/006 , C12G1/0203 , C12J1/00 , C12N9/16 , C12N9/2402 , C12N9/2437 , C12N9/244 , C12N9/60 , C12P7/06 , C12Y302/01004 , C12Y302/01006 , C12Y302/01015 , Y02E50/17
摘要： The present invention relates to a composite yeast for high concentration alcohol fermentation, which includes thermostable Saccharomyces cerevisiae, acid protease, phytase, cellulose, β-glucanase, and pectinase, and is suitable for the high concentration alcohol fermentation for various raw materials. In addition to normal fermentation, the composite yeast of the present invention can degrade the raw materials, increase the nutrient ingredients in the mash, promote the growth of yeast and provide stress tolerance protection.
摘要翻译：本发明涉及一种用于高浓度酒精发酵的复合酵母，其包括耐热酿酒酵母，酸性蛋白酶，植酸酶，纤维素，葡聚糖酶和果胶酶，适用于各种原料的高浓度酒精发酵。除正常发酵外，本发明的复合酵母可以降解原料，增加醪液中的营养成分，促进酵母生长，提供耐应力保护。

7. 发明申请

US20100184196A1 COMPOSITE YEAST SUITABLE FOR HIGH CONCENTRATION ALCOHOL FERMENTATION FROM SUGAR-CONTAINING RAW MATERIALS 有权
标题翻译：用于含有原料的高浓度酒精发酵的复合材料YEAST
公开(公告)号：US20100184196A1
公开(公告)日：2010-07-22
申请号：US12668578
申请日：2008-07-30
申请人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Zhijun Li , Daiwu Liu
发明人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Zhijun Li , Daiwu Liu
IPC分类号： C12N1/16 , C12P7/06
CPC分类号： C12P39/00 , C12N1/16 , C12P7/14 , Y02E50/17
摘要： The present invention relates to a composite yeast suitable for high concentration alcohol fermentation from sugar-containing raw materials, characterizing in that the composite yeast comprises any kind of dried yeast selected from Brewers yeast Saccharomyces cerevisiae Hansen of Saccharomyces cerevisiae, grape wine yeast Saccharomyces uvarum Beijerinek, and nutritious materials which are necessary for yeast growth, the nutritious materials include: the dried yeast 40-70 parts by weight, a nitrogen source 20-40 parts by weight, a phosphorous source 5-10 parts by weight, an other inorganic salt 2.5-5 parts by weight, a trace vitamin 1-2.5 parts by weight and a bacteriostatic 0.5-1.2 parts by weight. The present invention further relates to a method for preparation of the composite yeast suitable for high concentration alcohol fermentation from sugar-containing raw materials. Using the composite yeast of the present invention to proceed with sugar fermentation can increase fermentation alcoholicity, decrease residual sugar content, and allows the final alcoholicity of standard raw material, such as sucrose to attain to 14.5-15.5% v/v, and the amount of the residual reducing sugar in the fermentation mash is 0-0.1 wt %.
摘要翻译：本发明涉及适用于含糖原料的高浓度酒精发酵的复合酵母，其特征在于复合酵母包含任何种类的干酵母，其选自酿酒酵母酿酒酵母酿酒酵母Hansen，酿酒酵母酿酒酵母Beijerinek 和酵母生长所必需的营养物质，营养物质包括：干燥酵母40-70重量份，氮源20-40重量份，磷源5-10重量份，其它无机盐 2.5-5重量份，微量维生素1-2.5重量份，抑菌剂0.5-1.2重量份。本发明还涉及一种适用于含糖原料的高浓度酒精发酵的复合酵母的制备方法。使用本发明的复合酵母进行糖发酵可以提高发酵酒精度，降低残留糖含量，并允许标准原料如蔗糖的最终酒精度达到14.5-15.5％v / v，的发酵麦芽汁中的残留还原糖为0-0.1重量％。

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