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1. 发明授权

US08263374B2 Composite yeast suitable for high concentration alcohol fermentation from sugar-containing raw materials 有权
标题翻译：复合酵母适用于含糖原料的高浓度酒精发酵
公开(公告)号：US08263374B2
公开(公告)日：2012-09-11
申请号：US12668578
申请日：2008-07-30
申请人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Zhijun Li , Daiwu Liu
发明人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Zhijun Li , Daiwu Liu
IPC分类号： C12P7/14 , C12P7/06 , C12N1/00 , C12N1/12 , A01N63/04
CPC分类号： C12P39/00 , C12N1/16 , C12P7/14 , Y02E50/17
摘要： The present invention relates to a composite yeast suitable for high concentration alcohol fermentation from sugar-containing raw materials, characterizing in that the composite yeast comprises any kind of dried yeast selected from Brewers yeast Saccharomyces cerevisiae Hansen of Saccharomyces cerevisiae, grape wine yeast Saccharomyces uvarum Beijerinek, and nutritious materials which are necessary for yeast growth, the nutritious materials include: the dried yeast 40-70 parts by weight, a nitrogen source 20-40 parts by weight, a phosphorous source 5-10 parts by weight, an other inorganic salt 2.5-5 parts by weight, a trace vitamin 1-2.5 parts by weight and a bacteriostatic 0.5-1.2 parts by weight. The present invention further relates to a method for preparation of the composite yeast suitable for high concentration alcohol fermentation from sugar-containing raw materials. Using the composite yeast of the present invention to proceed with sugar fermentation can increase fermentation alcoholicity, decrease residual sugar content, and allows the final alcoholicity of standard raw material, such as sucrose to attain to 14.5-15.5% v/v, and the amount of the residual reducing sugar in the fermentation mash is 0-0.1 wt %.
摘要翻译：本发明涉及适用于含糖原料的高浓度酒精发酵的复合酵母，其特征在于复合酵母包含任何种类的干酵母，其选自酿酒酵母酿酒酵母酿酒酵母Hansen，酿酒酵母酿酒酵母Beijerinek 和酵母生长所必需的营养物质，营养物质包括：干燥酵母40-70重量份，氮源20-40重量份，磷源5-10重量份，其它无机盐 2.5-5重量份，微量维生素1-2.5重量份，抑菌剂0.5-1.2重量份。本发明还涉及一种适用于含糖原料的高浓度酒精发酵的复合酵母的制备方法。使用本发明的复合酵母进行糖发酵可以提高发酵酒精度，降低残留糖含量，并允许标准原料如蔗糖的最终酒精度达到14.5-15.5％v / v，的发酵麦芽汁中的残留还原糖为0-0.1重量％。

2. 发明申请

US20110171710A1 METHOD FOR PRODUCING CELLULOSIC ETHANOL 审中-公开
标题翻译：生产纤维素乙醇的方法
公开(公告)号：US20110171710A1
公开(公告)日：2011-07-14
申请号：US13054851
申请日：2009-09-27
申请人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Zhijun Li , Daiwu Liu
发明人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Zhijun Li , Daiwu Liu
IPC分类号： C12P7/10
CPC分类号： C12P7/10 , C12P7/065 , Y02E50/16 , Y02E50/17
摘要： A method for producing cellulosic ethanol is disclosed, which comprises the following steps: adding the medium containing the source materials of cellulose and/or hemicellulose to fermentation reaction vessel; adding cellulase to fermentation reaction vessel, and inoculating Candida lusitaniae; running simultaneous saccharification fermentation (SSFs) with the combined action of cellulose and Candida lusitaniae, and obtaining cellulosic ethanol by separation. Candida lusitaniae used in SSFs in present invention has higher ethanol tolerance and a higher ethanol production rate, it can use cellulose and hemicellulose as source materials and effectively produce cellulosic ethanol with cellulase in SSFs.
摘要翻译：公开了一种生产纤维素乙醇的方法，包括以下步骤：向发酵反应容器中加入含有纤维素和/或半纤维素原料的培养基; 向发酵反应容器中加入纤维素酶，接种假丝酵母; 通过纤维素和假丝酵母（Candida lusitaniae）的联合作用同时进行糖化发酵（SSFs），并通过分离获得纤维素乙醇。本发明SSF中使用的假丝酵母具有较高的乙醇耐受性和较高的乙醇生产率，可以使用纤维素和半纤维素作为原料，并在SSF中有效地生产纤维素酶纤维素乙醇。

3. 发明申请

US20080187987A1 Composite Yeast Suitable For High Concentration Alcohol Fermentation 有权
标题翻译：复合酵母适用于高浓度酒精发酵
公开(公告)号：US20080187987A1
公开(公告)日：2008-08-07
申请号：US11914475
申请日：2005-12-30
申请人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Zhijun Li , Daiwu Liu
发明人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Zhijun Li , Daiwu Liu
IPC分类号： C12N1/00
CPC分类号： C12N1/18 , C12C12/006 , C12G1/0203 , C12J1/00 , C12N9/16 , C12N9/2402 , C12N9/2437 , C12N9/244 , C12N9/60 , C12P7/06 , C12Y302/01004 , C12Y302/01006 , C12Y302/01015 , Y02E50/17
摘要： The present invention relates to a composite yeast for high concentration alcohol fermentation, which includes thermostable Saccharomyces cerevisiae, acid protease, phytase, cellulose, β-glucanase, and pectinase, and is suitable for the high concentration alcohol fermentation for various raw materials. In addition to normal fermentation, the composite yeast of the present invention can degrade the raw materials, increase the nutrient ingredients in the mash, promote the growth of yeast and provide stress tolerance protection.
摘要翻译：本发明涉及一种用于高浓度酒精发酵的复合酵母，其包括耐热酿酒酵母，酸性蛋白酶，植酸酶，纤维素，β-葡聚糖酶和果胶酶，并且适用于各种原料的高浓度酒精发酵。除正常发酵外，本发明的复合酵母可以降解原料，增加醪液中的营养成分，促进酵母生长，提供耐应力保护。

4. 发明授权

US08828187B2 Method for processing lignocellulose material 有权
标题翻译：木质纤维素材料的处理方法
公开(公告)号：US08828187B2
公开(公告)日：2014-09-09
申请号：US13445894
申请日：2012-04-12
申请人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Daiwu Liu , Jincheng Lei , Zhijun Li
发明人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Daiwu Liu , Jincheng Lei , Zhijun Li
IPC分类号： D21C1/00 , C12P19/14
CPC分类号： C12P19/14 , C12P19/02 , Y02E50/16
摘要： A method for processing a lignocellulose material, comprises the following steps: (1) crushing and sieving the lignocellulose material, and collecting granules with a particle size of between 0.08 and 0.1 mm; (2) mixing the granules obtained in step (1) with water, and dispersing through a colloid mill to yield a suspension with a particle size of 40-80 μm; (3) homogenizing the suspension obtained in the step (2) under high pressure to have a particle size of between 10 and 40 μm; and (4) buffering the suspension obtained in the step (3) with a buffer solution of sodium acetate and acetic-acid, adding cellulase, β-glucosidase, and xylanase, and performing zymolysis for 36-72 hours.
摘要翻译：一种处理木质纤维素材料的方法，包括以下步骤：（1）对木质纤维素材料进行破碎筛分，并收集粒度在0.08至0.1mm之间的颗粒; （2）将步骤（1）中获得的颗粒与水混合，并通过胶体磨分散，得到粒径为40-80μm的悬浮液; （3）在高压下使步骤（2）中得到的悬浮液均匀化，使其粒径在10〜40μm之间; 和（4）用乙酸钠和乙酸缓冲溶液缓冲步骤（3）获得的悬浮液，加入纤维素酶，葡糖苷酶和木聚糖酶，并进行酶解36-72小时。

5. 发明授权

US08697425B2 Composite yeast suitable for high concentration alcohol fermentation 有权
标题翻译：复合酵母适用于高浓度酒精发酵
公开(公告)号：US08697425B2
公开(公告)日：2014-04-15
申请号：US11914475
申请日：2005-12-30
申请人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Zhijun Li , Daiwu Liu
发明人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Zhijun Li , Daiwu Liu
IPC分类号： C12N1/18
CPC分类号： C12N1/18 , C12C12/006 , C12G1/0203 , C12J1/00 , C12N9/16 , C12N9/2402 , C12N9/2437 , C12N9/244 , C12N9/60 , C12P7/06 , C12Y302/01004 , C12Y302/01006 , C12Y302/01015 , Y02E50/17
摘要： The present invention relates to a composite yeast for high concentration alcohol fermentation, which includes thermostable Saccharomyces cerevisiae, acid protease, phytase, cellulose, β-glucanase, and pectinase, and is suitable for the high concentration alcohol fermentation for various raw materials. In addition to normal fermentation, the composite yeast of the present invention can degrade the raw materials, increase the nutrient ingredients in the mash, promote the growth of yeast and provide stress tolerance protection.
摘要翻译：本发明涉及一种用于高浓度酒精发酵的复合酵母，其包括耐热酿酒酵母，酸性蛋白酶，植酸酶，纤维素，葡聚糖酶和果胶酶，适用于各种原料的高浓度酒精发酵。除正常发酵外，本发明的复合酵母可以降解原料，增加醪液中的营养成分，促进酵母生长，提供耐应力保护。

6. 发明申请

US20100184196A1 COMPOSITE YEAST SUITABLE FOR HIGH CONCENTRATION ALCOHOL FERMENTATION FROM SUGAR-CONTAINING RAW MATERIALS 有权
标题翻译：用于含有原料的高浓度酒精发酵的复合材料YEAST
公开(公告)号：US20100184196A1
公开(公告)日：2010-07-22
申请号：US12668578
申请日：2008-07-30
申请人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Zhijun Li , Daiwu Liu
发明人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Zhijun Li , Daiwu Liu
IPC分类号： C12N1/16 , C12P7/06
CPC分类号： C12P39/00 , C12N1/16 , C12P7/14 , Y02E50/17
摘要： The present invention relates to a composite yeast suitable for high concentration alcohol fermentation from sugar-containing raw materials, characterizing in that the composite yeast comprises any kind of dried yeast selected from Brewers yeast Saccharomyces cerevisiae Hansen of Saccharomyces cerevisiae, grape wine yeast Saccharomyces uvarum Beijerinek, and nutritious materials which are necessary for yeast growth, the nutritious materials include: the dried yeast 40-70 parts by weight, a nitrogen source 20-40 parts by weight, a phosphorous source 5-10 parts by weight, an other inorganic salt 2.5-5 parts by weight, a trace vitamin 1-2.5 parts by weight and a bacteriostatic 0.5-1.2 parts by weight. The present invention further relates to a method for preparation of the composite yeast suitable for high concentration alcohol fermentation from sugar-containing raw materials. Using the composite yeast of the present invention to proceed with sugar fermentation can increase fermentation alcoholicity, decrease residual sugar content, and allows the final alcoholicity of standard raw material, such as sucrose to attain to 14.5-15.5% v/v, and the amount of the residual reducing sugar in the fermentation mash is 0-0.1 wt %.
摘要翻译：本发明涉及适用于含糖原料的高浓度酒精发酵的复合酵母，其特征在于复合酵母包含任何种类的干酵母，其选自酿酒酵母酿酒酵母酿酒酵母Hansen，酿酒酵母酿酒酵母Beijerinek 和酵母生长所必需的营养物质，营养物质包括：干燥酵母40-70重量份，氮源20-40重量份，磷源5-10重量份，其它无机盐 2.5-5重量份，微量维生素1-2.5重量份，抑菌剂0.5-1.2重量份。本发明还涉及一种适用于含糖原料的高浓度酒精发酵的复合酵母的制备方法。使用本发明的复合酵母进行糖发酵可以提高发酵酒精度，降低残留糖含量，并允许标准原料如蔗糖的最终酒精度达到14.5-15.5％v / v，的发酵麦芽汁中的残留还原糖为0-0.1重量％。

7. 发明申请

US20110165289A1 FEED PREMIX CONTAINING MULTIPLE TRACE ELEMENTS AND PREPARING METHOD THEREOF 审中-公开
标题翻译：含有多种追踪元素的饲料及其制备方法
公开(公告)号：US20110165289A1
公开(公告)日：2011-07-07
申请号：US13054894
申请日：2009-09-23
申请人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Bin Tan
发明人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Bin Tan
IPC分类号： A23K1/175 , A23K1/00
CPC分类号： A23K50/30 , A23K10/16 , A23K20/30 , A23K50/75 , A23V2002/00 , A23V2250/1588 , A23V2250/1592 , A23V2250/1642 , A23V2250/1612
摘要： A feed premix containing multiple trace elements and preparing method thereof, the feed premix contains trace elements of copper, iron, manganese and zinc, and yeast autolysate, the proportion of the four trace elements of which depending one the feeding standards of various animals. The method includes reacting the salts of copper, iron, manganese and zinc with a yeast autolysate.
摘要翻译：含有多种微量元素的饲料预混物及其制备方法，饲料预混物含有铜，铁，锰和锌的痕量元素，酵母自溶，其中四种微量元素的比例取决于各种动物的摄食标准。该方法包括使铜，铁，锰和锌的盐与酵母自溶物反应。

8. 发明申请

US20100189871A1 BIOTOXIN SORBENT AND METHOD FOR PREPARING THE SAME 审中-公开
标题翻译：生物素SORBENT及其制备方法
公开(公告)号：US20100189871A1
公开(公告)日：2010-07-29
申请号：US12668564
申请日：2008-08-06
申请人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Bin Tan , Jinlin Zhu
发明人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Bin Tan , Jinlin Zhu
IPC分类号： A23K1/175 , C12P19/04 , B01J20/12 , B01J20/22
CPC分类号： B01J20/28004 , A23K20/163 , A23K20/28 , A23K50/10 , A23K50/70 , A23K50/80 , B01J20/12 , B01J20/16 , B01J20/24 , B01J2220/46
摘要： The present invention provides a combination used for absorbing toxins in feed, comprising 1-10% by weight of clay and 90-99% by weight of yeast manna oligosaccharide, wherein the yeast manna oligosaccharide is extract of yeast cell wall which is separated from Saccharomyces cerevisiae. The biotoxin sorbent product can be used to feed any animals, including livestock, fowls, marine lives and ruminants. When mixing with feed, owing to its strong toxin adsorption capability, this absorbent may reduce the amount of toxins entering bodies of animals, thereby improving productivity and health of animals, reducing diseases caused by toxins.
摘要翻译：本发明提供了用于吸收饲料中毒素的组合物，其包含1-10重量％的粘土和90-99重量％的酵母甘露寡糖，其中所述酵母甘露寡糖是酵母细胞壁的提取物，其从酵母菌啤酒酵母生物毒素吸附剂产品可用于饲养任何动物，包括牲畜，家禽，海洋生物和反刍动物。当与饲料混合时，由于其强的毒素吸附能力，该吸收剂可以减少进入动物体内的毒素的量，从而提高动物的生产力和健康，减少由毒素引起的疾病。

9. 发明授权

US08883137B2 Compositions comprising selenium-rich yeast and yeast beta-glucan 有权
标题翻译：包含富硒酵母和酵母β-葡聚糖的组合物
公开(公告)号：US08883137B2
公开(公告)日：2014-11-11
申请号：US12264331
申请日：2008-11-04
申请人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Yan Zhang , Yamin Zhu
发明人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Yan Zhang , Yamin Zhu
IPC分类号： A61K38/44 , A01N59/02 , A61K36/06 , A23L1/303 , A23L1/30 , A23L1/302 , A23L1/304
CPC分类号： A23L1/3016 , A23L33/105 , A23L33/14 , A23L33/15 , A23L33/16 , A61K36/06
摘要： A nutritional composition comprising by weight: between 0.05 and 30% selenium-enriched yeast; between 1 and 90% yeast beta-glucan; between 0.5 and 60% Vitamin C; and between 0.5 and 40% Vitamin E. The composition features anti-oxidative, anti-aging, cancer-preventing, and immune-stimulating properties.
摘要翻译：一种营养组合物，其按重量计：含有富含富含酵母的0.05-30％的酵母; 1至90％酵母β-葡聚糖; 维生素C在0.5至60％之间; 和维生素E的0.5至40％之间。该组合物具有抗氧化，抗衰老，防癌和免疫刺激的特性。

10. 发明申请

US20110045545A1 METHOD AND PREPARING GLUCAN AND MANNAN, GLUCAN PREPARATION AND MANNAN PREPARATION PRODUCED THEREBY AND USE THEREOF 有权
标题翻译：方法和制备葡萄糖和甘露糖，葡萄糖的制备和制备及其制备及其使用
公开(公告)号：US20110045545A1
公开(公告)日：2011-02-24
申请号：US12990206
申请日：2008-12-31
申请人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Yan Zhang
发明人： Xuefeng Yu , Zhihong Li , Minghua Yu , Juan Yao , Yan Zhang
IPC分类号： C12P19/04
CPC分类号： C12P19/04 , C08B37/0003 , C08B37/0087
摘要： The present invention provides a method for extracting glucan and mannan from the cell wall of a microorganism. Specifically, the method of the present invention in one embodiment comprises the steps of: a) treating the cells of the microorganism with an alkaline protease and an mannanase; b) separating the mixture from step a) into a heavy phase and a light phase; c) drying the heavy phase obtained from step b), obtaining the glucan preparation; and d) drying the light phase obtained from step b), obtaining the mannan preparation. Optionally, in the step c), the heavy phase obtained from step b) may be treated sequentially with an alkali and an acid, and separated again into a heavy phase and a light phase. The heavy phase is dried, obtaining the glucan preparation. The present invention further relates to the glucan preparation and mannan preparation produced thereby, and the uses thereof.
摘要翻译：本发明提供了从微生物细胞壁提取葡聚糖和甘露聚糖的方法。具体地说，本发明的一个实施方案包括以下步骤：a）用碱性蛋白酶和甘露聚糖酶处理微生物细胞; b）将混合物从步骤a）分离成重相和轻相; c）干燥从步骤b）获得的重相，得到葡聚糖制剂; 和d）干燥从步骤b）得到的轻相，得到甘露聚糖制剂。任选地，在步骤c）中，可以用碱和酸依次处理从步骤b）获得的重相，并再次分离成重相和轻相。将重相干燥，得到葡聚糖制剂。本发明还涉及由此制备的葡聚糖制剂和甘露聚糖制剂及其用途。

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