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1. 发明申请

US20120102593A1 Use of a Histone Deacetylase Gene OsHDT1 in Enhancing Rice Heterosis 审中-公开
标题翻译：使用组蛋白脱乙酰酶基因OsHDT1来增强水稻杂种优势
公开(公告)号：US20120102593A1
公开(公告)日：2012-04-26
申请号：US13266914
申请日：2010-04-29
申请人： Daoxiu Zhou , Chen Li , Limin Huang
发明人： Daoxiu Zhou , Chen Li , Limin Huang
IPC分类号： A01H5/00 , C12N5/10 , A01H1/06 , A01H5/10
CPC分类号： C12N9/16 , C12N15/8261 , C12N15/8289 , Y02A40/146
摘要： A histone deacetylase gene OsHDT1 which enhances utilization of heterosis in rice is isolated and cloned, said gene consisting of: 1) the DNA sequence of positions 1-894 in SEQ ID NO:1 in the Sequence listing; or 2) a DNA sequence which encodes the same protein as that encoded by the DNA sequence of 1). The histone deacetylase gene OsHDT1 is associated with enhanced utilization of heterosis in rice. When the transgenic plants having no phenotype were crossed with “Zhenshan 97A”, the F1 overexpression hybrid plants have obviously earlier flowering period than F1 wild-type hybrid plants. Moreover, the RNAi inhibition hybrid plants have a decreased seed setting rate compared to the negative control hybrid plants, while the parent RNAi inhibition plants and negative control plants shows no evident difference in the trait of seed setting rate. Western blotting revealed that the gene is capable of deacetylating histone, mainly at H4K16 site. In addition, there exist significant differences in the level of histone modification among “Minghui 63”, “Zhenshan 97” and “Shanyou63”.
摘要翻译：分离并克隆提高水稻利用率的组蛋白脱乙酰酶基因OsHDT1，该基因由以下组成：1）序列表中SEQ ID NO：1中1-894位的DNA序列; 或2）编码与由1）的DNA序列编码的蛋白相同的蛋白质的DNA序列。组蛋白脱乙酰酶基因OsHDT1与水稻中杂种优势的利用有关。当没有表型的转基因植物与“珍山97A”杂交时，F1过表达杂交植物的开花期明显比F1野生型杂种植物早。此外，与阴性对照杂交植物相比，RNAi抑制杂交植物的种子定形率降低，而亲本RNAi抑制植物和阴性对照植物在种子定形率的性状上没有显示出明显的差异。蛋白质印迹显示该基因能够脱乙酰化组蛋白，主要在H4K16位点。此外，“明辉63”，“贞山97”，“汕优63”组蛋白修饰水平存在显着差异。

2. 发明申请

US20110160444A1 Cloning Transcription Factor Gene OsWOX20 That Regulates The Growth and Development of Monocotyledon's Root and Uses Thereof 审中-公开
标题翻译：克隆转录因子基因OsWOX20调节单子叶根的生长发育及其应用
公开(公告)号：US20110160444A1
公开(公告)日：2011-06-30
申请号：US12668391
申请日：2008-06-26
申请人： Yu Zhao , Daoxiu Zhou
发明人： Yu Zhao , Daoxiu Zhou
IPC分类号： C07H21/04
CPC分类号： C12N15/8227 , C07K14/415 , C12N15/8261 , Y02A40/146
摘要： he present disclosure pertains to the field of plant genetic engineering. Specifically, the present disclosure relates to isolation and cloning, function verification, and use of a transcription factor gene OsWOX20 that regulates growth and development of roots of monocotyledons. According to the present disclosure, a transcription factor gene Os WOX20 DNA is isolated which regulates growth and development of roots of rice, and has (a) the DNA sequence of positions 1-786 in SEQ ID NO: 1 in the Sequence Listing, or (b) a DNA sequence that encodes the same protein as that encoded by the DNA sequence of (a). The promoter according to the present disclosure has the DNA sequence of positions 1-2078 in SEQ ID NO: 3 in the Sequence Listing. The cloned gene sequence is used to transform a rice variety and transgenic rice plants showing markedly improved growth and development of roots are obtained. The promoter drives the specific expression of a reporter gene in the roots of rice. The present disclosure shows that the cloned target gene and its promoter have a great prospect in their use in breeding transgenic plants having improved growth and development of roots.
摘要翻译：他的揭示涉及植物基因工程领域。具体地，本公开涉及分离和克隆，功能验证和调节单子叶根生长和发育的转录因子基因OsWOX20的使用。根据本公开，分离调节水稻根系的生长发育的转录因子Os WOX20DNA，并且（a）序列表中SEQ ID NO：1中的位置1-786的DNA序列，或（b）编码与（a）的DNA序列编码的蛋白质相同的蛋白质的DNA序列。根据本公开的启动子具有序列表中SEQ ID NO：3中的位置1-2078的DNA序列。克隆的基因序列用于转化水稻品种，并获得显着改善生长和转基因发育的转基因水稻。启动子驱动水稻根中报道基因的特异性表达。本公开显示克隆的靶基因及其启动子在用于育种具有改善的根的生长和发育的转基因植物中具有很大的前景。

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