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    • 1. 发明申请
    • HIGH YIELD PERTUSSIS VACCINE PRODUCTION STRAIN AND METHOD FOR MAKING SAME
    • 高产量PERTUSSIS VACCINE生产菌株及其制备方法
    • US20060154354A1
    • 2006-07-13
    • US10312272
    • 2001-06-26
    • Tod MerkelJerry KeithXiaoming Yang
    • Tod MerkelJerry KeithXiaoming Yang
    • C12N1/21
    • C12N1/20C07K14/235C12N15/74
    • The present invention provides a vaccine production strain of Bordetella bronchiseptica that produces a pertussis toxin in high yield. The present invention provides a method for creating a Bordetella bronchiseptica cell line which produces a Bordetella pertussis toxin comprising the steps of introducing a plasmid containing a DNA encoding antibiotic resistance into a Bordetella bronchiseptica strain, selecting for isolates in which the DNA encoding antibiotic resistance is recombinantly incorporated into the chromosome in place of the Bordetella bronchiseptica toxin gene, introducing a plasmid containing DNA encoding subunits of the Bordetella pertussis toxins into the Bordetella bronchiseptica isolates; and, selecting for isolates in which DNA encoding Bordetella pertussis toxin subunit is recombinantly incorporated into the chromosome, the resulting cells producing the Bordetella pertussis toxin. The present invention further provides a method for creating a Bordetella bronchiseptica cell line which produces a Bordetella pertussis toxin and does not express filamentous hemagglutinin.
    • 本发明提供了以高产率产生百日咳毒素的支气管炎博德特氏菌疫苗生产菌株。 本发明提供了一种产生博德特氏菌百日咳杆菌毒素的博德特氏菌细胞系的方法,包括将含有编码抗生素抗性的DNA的质粒导入支气管炎博德特氏菌菌株,选择其中编码抗生素抗性的DNA重组的分离株 引入染色体以代替支气管炎博德特氏菌毒素基因,将含有编码百日咳杆菌毒素的亚基的DNA的质粒引入支气管炎博德特菌分离株; 并且选择其中编码百日咳博德特氏菌毒素亚基的DNA重组并入染色体的分离物,所得细胞产生百日咳博德特氏菌毒素。 本发明还提供一种产生支气管炎博德特氏菌细胞系的方法,其产生百日咳杆菌毒素并且不表达丝状血凝素。
    • 3. 发明授权
    • Hot water soluble disposable films, fabrics and articles
    • 热水溶性一次性薄膜,织物和制品
    • US5658977A
    • 1997-08-19
    • US583770
    • 1996-01-11
    • Xiaoming YangRobert J. Petcavich
    • Xiaoming YangRobert J. Petcavich
    • C08L29/04C08L67/00C08L67/02C08L67/04C08L67/06C08G63/48
    • C08L67/02C08L29/04C08L67/04C08L67/06C08L67/00
    • Cold water insoluble, hot water soluble and/or dispersible, disposable films, fabrics and other useful articles of manufacture are formed from a miscible polymeric resin blend comprised of cold water soluble partially hydrolyzed polyvinyl alcohol, cold water insoluble hot water disintegrable aliphatic polyester, and minor proportions of processing and performance aids. The aliphatic polyester has a melt temperature above the normal body temperature of a human (37 degrees C.; 98.6 degrees F.) and is present in the resin blend at a concentration sufficient to constitute the continuous phase of the blend, with the polyvinyl alcohol constituting a discontinuous phase of the blend. The aliphatic polyester thus renders the resin blend, and the partially hydrolyzed polyvinyl alcohol in the blend, cold water insoluble and determines the temperature at which articles formed from the blend will be subject to dissolution in an aqueous bath and subsequent disposal in an environmentally and ecologically sound fashion.
    • 冷水不溶性,热水溶性和/或可分散的一次性膜,织物和其它有用的制品由可混溶的聚合树脂混合物形成,所述共混物由冷水溶性部分水解的聚乙烯醇,冷水不溶性热水可分解的脂族聚酯和 次要比例的加工和性能辅助。 脂肪族聚酯具有高于人体正常体温(37℃; 98.6华氏度)的熔体温度,并且以足以构成共混物的连续相的浓度存在于树脂共混物中,与聚乙烯醇 构成共混物的不连续相。 因此,脂族聚酯使树脂共混物和共混物中的部分水解的聚乙烯醇,冷水不溶,并确定由共混物形成的制品将在水浴中溶解的温度,随后在环境和生态环境中处理 声音时尚。
    • 7. 发明授权
    • Alternating current contactor
    • 交流接触器
    • US06472963B1
    • 2002-10-29
    • US09752394
    • 2000-12-28
    • Chuanting LiuXiaoming YangHenming YanYinghao Li
    • Chuanting LiuXiaoming YangHenming YanYinghao Li
    • H01H6702
    • H01H50/042H01H50/026H01H50/305
    • The utility model discloses an alternating current contactor, including a foundation-support and a pedestal. A yoke iron on which mounts a coil unit is set inside the said foundation-support, and an armature iron and a contact unit are set in the said pedestal. The characteristics are that one side of the said foundation-support is open, and the coil unit and the yoke iron are constituted into a whole via a supporting board and buffer springs, and then it is fixed on the convex face of pulling part of the said foundation-support. A baffle plate with openings is on the top of the foundation-support, and a repelling force spring is installed between the baffle plate on the top of the foundation support and the armature iron. The coil unit can be easily inserted into or pulled out from the opening of the foundation-support, so that either repairing or replacing the coil unit become rather convenient.
    • 本实用新型公开了一种交流接触器,包括基座支架和基座。 将安装有线圈单元的轭铁设置在所述基座支撑件内部,并且将电枢铁和接触单元设置在所述基座中。 特点是所述基座支架的一侧是敞开的,线圈单元和轭铁通过支撑板和缓冲弹簧构成整体,然后固定在拉伸部分的凸面上 说基础支持。 具有开口的挡板位于基座支撑件的顶部,并且排斥力弹簧安装在基座支撑件顶部的挡板和衔铁之间。 线圈单元可以容易地插入基座支撑件的开口中或从基座支撑件的开口拉出,使得修理或更换线圈单元变得相当方便。