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    • 83. 发明公开
    • 사람 에리트로포이에틴을 생산하는 형질전환 복제소 및이의 생산 방법
    • 转基因克隆生产人ERYTHROPOIETIN及其生产方法
    • KR1020040101793A
    • 2004-12-03
    • KR1020030033582
    • 2003-05-27
    • 재단법인서울대학교산학협력재단
    • 황우석이병천강성근정의배조종기김순웅박을순장구박희정
    • C12N5/16
    • C12N15/8771A01K67/0273A01K2227/101A01K2267/01A61D19/04C12N15/8509C12N2015/8518C12N2517/00
    • PURPOSE: Transgenic cloned cow producing human erythropoietin and a method for producing the same transgenic cloned cow are provided, which method allows economic and effective production of various biomedicines, and facilitates production of human erythropoietin in cow's milk. CONSTITUTION: The nuclear transfer embryo produced by a fusion of a cow-derived enucleated oocyte with a nucleus of a cow-derived somatic cell transfected with a gene encoding human erythropoietin is provided, wherein the cow is an adult; the nuclear transfer embryo is SNU-B4(KCTC 10450BP). The method for producing a transgenic cloned cow expressing human erythropoietin in the mammary gland comprises the steps of: (a) introducing a gene encoding human erythropoietin into a cow-derived somatic cell line to prepare a nuclear donor cell; (b) removing cumulus cells surrounding a recipient oocyte and then the cytoplasm including the first polar body from the oocyte to prepare a cow-derived enucleated recipient oocyte; (c) transferring the transfected nuclear donor cell into the enucleated recipient oocyte and carrying out cell fusion to generate nuclear transfer embryos; and (d) transplanting the nuclear transfer embryo into a surrogate mother cow to produce live offspring.
    • 目的:提供转基因克隆的牛产生人促红细胞生成素和生产相同转基因克隆牛的方法,该方法允许经济有效地生产各种生物医学,并促进牛奶中人促红细胞生成素的产生。 构成:提供由牛衍生的去核卵母细胞与用编码人促红细胞生成素的基因转染的牛衍生的体细胞的细胞核融合而产生的核转移胚,其中,牛是成年人; 核转移胚是SNU-B4(KCTC 10450BP)。 用于产生在乳腺中表达人促红细胞生成素的转基因克隆牛的方法包括以下步骤:(a)将编码人促红细胞生成素的基因导入牛衍生的体细胞系以制备核供体细胞; (b)去除卵母细胞周围的卵丘细胞,然后从卵母细胞中除去包含第一极体的细胞质,以制备牛衍生的去核受体卵母细胞; (c)将转染的核供体细胞转移到去核的受体卵母细胞中并进行细胞融合以产生核转移胚胎; 和(d)将核转移胚胎移植到替代母牛中以产生活的后代。