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61. 发明授权

US08603741B2 Single molecule sequencing with two distinct chemistry steps 有权
标题翻译：具有两个不同化学步骤的单分子测序
公开(公告)号：US08603741B2
公开(公告)日：2013-12-10
申请号：US13022926
申请日：2011-02-08
申请人： Robin Emig , Lei Jia , Jeremiah Hanes , Lubomir Sebo
发明人： Robin Emig , Lei Jia , Jeremiah Hanes , Lubomir Sebo
IPC分类号： C12Q1/68 , C07H19/04
CPC分类号： C12Q1/6869 , C07H19/10 , C07H19/11 , C07H19/20 , C07H19/213 , C12Q1/6874 , C12Q2521/301 , C12Q2525/186 , C12Q2563/107 , C12Q2537/157 , C12Q2521/319 , C12Q2521/525
摘要： Methods, Compositions, and Systems are provided for nucleic acid sequencing where the sequential incorporation of nucleotides uses two distinct chemical steps. A plurality of nucleotide analogs, each having a labeled leaving group at its 3′ hydroxyl can be sequentially added to a growing strand in the presence of a selective cleaving activity that cleaves the 3′ hydroxyl leaving group preferentially after it has been incorporated. The selective cleaving agent can comprise an exonuclease activity, and the exonuclease activity can be a polymerase-associated exonuclease activity. Nucleotide analogs having labels on both a cleavable polyphosphate portion and on a 3′ hydroxyl leaving group can provide signals characteristic of nucleotide analog incorporation. Systems having illumination optics, collection optics, and substrates observe signals from the labels as they are being incorporated into a growing nucleic acid strand, allowing for the sequencing of template nucleic acids.
摘要翻译：提供了用于核酸测序的方法，组合物和系统，其中连续掺入核苷酸使用两个不同的化学步骤。在选择性切割活性的存在下，可以将多个在其3'羟基上具有标记的离去基团的核苷酸类似物依次加入到生长链中，该选择性裂解活性优选在其引入后优先切割3'羟基离去基团。选择性切割剂可以包含核酸外切酶活性，并且核酸外切酶活性可以是聚合酶相关的外切核酸酶活性。在可切割聚磷酸酯部分和3'羟基离去基团上具有标记的核苷酸类似物可以提供核苷酸类似物掺入特征的信号。具有照明光学器件，收集光学元件和底物的系统在标记被并入生长的核酸链中观察信号，允许对模板核酸进行测序。

62. 发明授权

US08597881B2 Modified nucleotides 有权
标题翻译：修饰的核苷酸
公开(公告)号：US08597881B2
公开(公告)日：2013-12-03
申请号：US13281275
申请日：2011-10-25
申请人： John Milton , Xiaolin Wu , Mark Smith , Joseph Brennan , Colin Barnes , Xiaohai Liu , Silke Ruediger
发明人： John Milton , Xiaolin Wu , Mark Smith , Joseph Brennan , Colin Barnes , Xiaohai Liu , Silke Ruediger
IPC分类号： C12Q1/68 , C07H19/04
CPC分类号： C12Q1/6869 , C07H1/00 , C07H19/06 , C07H19/10 , C07H19/14 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q2525/186 , C12Q2535/113 , Y02P20/55 , C12Q2525/197
摘要： Provided herein is a method for replacing the allyl group in a compound of formula R—O-allyl, R2N(allyl), RNH(allyl), RN(allyl)2 or R—S-allyl with a hydrogen, using a transition metal and one or more phosphine ligands.
摘要翻译：本文提供的是使用过渡金属和一种或多种式（I）的式RO-烯丙基，R2N（烯丙基），RNH（烯丙基），RN（烯丙基）2或RS-烯丙基的化合物中的烯丙基膦配体。

63. 发明授权

US08486620B2 Polymeric carriers for immunohistochemistry and in situ hybridization 有权
公开(公告)号：US08486620B2
公开(公告)日：2013-07-16
申请号：US13066980
申请日：2011-04-29
申请人： Jerry W. Kosmeder , Casey A. Kernag , Donald Johnson , Christopher Bieniarz
发明人： Jerry W. Kosmeder , Casey A. Kernag , Donald Johnson , Christopher Bieniarz
IPC分类号： C12Q1/70 , C12Q1/68 , G01N33/53 , C07H19/04 , C07K2/00 , C07K16/00
CPC分类号： G01N33/581 , A61K47/6883 , A61K49/0058 , A61K49/0067 , C07K17/08 , G01N33/532 , G01N33/54353 , G01N33/544 , G01N33/545 , G01N33/56983 , G01N33/57492 , G01N33/582 , G01N33/587 , G01N33/588
摘要： Certain disclosed embodiments of the present invention concern the synthesis, derivatization, conjugation to immunoglobulins and signal amplification based on discrete, relatively short polymers having plural reactive functional groups that react with plural molecules of interest. Reactive functional groups, such as hydrazides, may be derivatized with a variety of detectable labels, particularly haptens. The remaining reactive functional groups may be conjugated directly to a specific binding molecule, such as to the oxidized carbohydrate of the Fc region of the antibody. Disclosed conjugates display large signal amplification as compared to those based on molecules derivatized with single haptens, and are useful for assay methods, particularly multiplexed assays.

64. 发明授权

US08450293B2 Synthesis and characterization of C8 analogs of c-di-GMP 有权
标题翻译： c-di-GMP的C8类似物的合成和表征
公开(公告)号：US08450293B2
公开(公告)日：2013-05-28
申请号：US13207169
申请日：2011-08-10
申请人： Roger A. Jones , Elizabeth Veliath
发明人： Roger A. Jones , Elizabeth Veliath
IPC分类号： A01N43/04 , A61K31/70 , C07H19/00 , C07H19/04 , C07H19/20
CPC分类号： C07H19/213
摘要： The invention provides analogs cyclic diguanosine monophosphate (c-di-GMP) having different substituents at the guanine C8 position.
摘要翻译：本发明提供在鸟嘌呤C8位置具有不同取代基的类似物环状二磷酸腺苷（c-di-GMP）。

65. 发明申请

US20130116207A1 Compositions and Methods for Treating Cancer 有权
标题翻译：治疗癌症的组合物和方法
公开(公告)号：US20130116207A1
公开(公告)日：2013-05-09
申请号：US13665184
申请日：2012-10-31
申请人： Eisai Inc.
发明人： Sergei Belyakov , Bridget Duvall , Dana Ferraris , Gregory Hamilton , Mark Vaal
IPC分类号： A61K31/7052 , A61K45/06 , C07H19/04
CPC分类号： A61K31/7052 , A61K31/7064 , A61K31/7068 , A61K45/06 , C07H19/04 , A61K2300/00
摘要： Provided herein are compounds used to inhibit the deamination enzyme responsible for the inactivation of therapeutic compounds, and methods of using them.
摘要翻译：本文提供了用于抑制负责灭活治疗化合物的脱氨酶的化合物及其使用方法。

66. 发明授权

US08394949B2 Multisignal labeling reagents, and processes and uses therefor 有权
公开(公告)号：US08394949B2
公开(公告)日：2013-03-12
申请号：US12399393
申请日：2009-03-06
申请人： Elazar Rabbani , Jannis G. Stavrianopoulos , James J. Donegan
发明人： Elazar Rabbani , Jannis G. Stavrianopoulos , James J. Donegan
IPC分类号： C07H19/04 , C07H7/06 , C07H5/04 , C07H1/00 , C12Q1/68 , C12P19/34
CPC分类号： C07H21/04
摘要： The present invention provides multisignal labeling reagents and these are useful in a number of biochemical applications, including the manufacture of biomolecular probes and their use in detecting or amplifying analyte-specific moieties.

67. 发明授权

US08357793B2 Label target and labeling reagents comprising backbones with at least two consecutive peptide bonds 有权
标题翻译：标记靶标和标记试剂，其包含具有至少两个连续肽键的骨架
公开(公告)号：US08357793B2
公开(公告)日：2013-01-22
申请号：US10763104
申请日：2004-01-22
申请人： Jannis G. Stavrianopoulos , Flazar Rabbani
发明人： Jannis G. Stavrianopoulos , Flazar Rabbani
IPC分类号： C07H19/04 , C07H21/00 , G01N33/532 , C12Q1/68
CPC分类号： C07H21/00 , C09B23/02 , C09B47/00 , C09B57/00 , Y10S436/80
摘要： This invention provides for labeling reagents, labeled targets and processes for preparing labeling reagents. The labeling reagents can take the form of cyanine dyes, xanthene dyes, porphyrin dyes, coumarin dyes or composite dyes. These labeling reagents are useful for labeling probes or targets, including nucleic acids and proteins. These reagents can be usefully applied to protein and nucleic acid probe based assays. They are also applicable to real-time detection processes.
摘要翻译：本发明提供标记试剂，标记的靶标和制备标记试剂的方法。标记试剂可以采用花青染料，呫吨染料，卟啉染料，香豆素染料或复合染料的形式。这些标记试剂可用于标记探针或靶标，包括核酸和蛋白质。这些试剂可用于基于蛋白质和基于核酸探针的测定。它们也适用于实时检测过程。

68. 发明授权

US08324180B2 Compositions and methods for treating cancer 有权
公开(公告)号：US08324180B2
公开(公告)日：2012-12-04
申请号：US12755106
申请日：2010-04-06
申请人： Sergei Belyakov , Bridget Duvall , Dana Ferraris , Gregory Hamilton , Mark Vaal
发明人： Sergei Belyakov , Bridget Duvall , Dana Ferraris , Gregory Hamilton , Mark Vaal
IPC分类号： A61K31/7052 , A61P35/00 , C07H19/04
CPC分类号： C07H19/04 , A61K31/7052
摘要： Provided herein are compounds used to inhibit the deamination enzyme responsible for the inactivation of therapeutic compounds, and methods of using them.

69. 发明申请

US20120294807A9 FLUORESCENT COMPOUNDS 审中-公开
公开(公告)号：US20120294807A9
公开(公告)日：2012-11-22
申请号：US13330993
申请日：2011-12-20
申请人： Greg Hermanson , Peter T. Czerney , Surbhi Desai , Matthias S. Wenzel , Boguslawa Dworecki , Frank G. Lehmann
发明人： Greg Hermanson , Peter T. Czerney , Surbhi Desai , Matthias S. Wenzel , Boguslawa Dworecki , Frank G. Lehmann
IPC分类号： G01N33/53 , C07K14/00 , C07K17/02 , C12N9/96 , C07H19/04 , C07H21/00 , C07K14/475 , C12N11/02 , C07K14/575 , C07H1/00 , G01N33/567 , A61K49/00 , C40B30/04 , G01N27/447 , C07D209/04
CPC分类号： C07D403/06 , C07D209/10
摘要： Compounds used as labels with properties comparable to known fluorescent compounds. The compounds can be conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific non-limiting examples of each are provided.

70. 发明授权

US08232050B2 Methods of using dyes in association with nucleic acid staining or detection and associated technology 有权
标题翻译：使用染料与核酸染色或检测及相关技术相结合的方法
公开(公告)号：US08232050B2
公开(公告)日：2012-07-31
申请号：US12854436
申请日：2010-08-11
申请人： Fei Mao , Wai-Yee Leung
发明人： Fei Mao , Wai-Yee Leung
IPC分类号： C12Q1/68 , C07H21/00 , C07H19/04
CPC分类号： C09B15/00 , C09B11/28 , C09B23/04 , C12Q1/6818 , G01N1/30 , G01N21/6486 , Y10S435/81 , Y10T436/143333 , C12Q2525/301
摘要： Methods of using dyes and associated technology are provided. A dye, such as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel staining application and/or a nucleic acid detection application. Such a dye and a salt that comprises an anion that is associated with a strong acid and a cation that is associated with a strong base may be used in such an application. A dimeric dye, such as a dimeric dye capable of forming a hairpin-like structure, may be used to stain and/or detect nucleic acids via a release-on-demand mechanism. A dimeric dye having low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, may be used to stain and/or detect nucleic acids.
摘要翻译：提供了使用染料和相关技术的方法。可以在核酸凝胶染色应用和/或核酸检测应用中使用染料，例如单体染料或二聚染料。这种染料和包含与强酸相关的阴离子和与强碱相关的阳离子的盐可用于这种应用中。可以使用二聚染料，例如能够形成发夹状结构的二聚染料，以通过按需释放机制对核酸进行染色和/或检测。在存在核酸时具有低背景荧光的二聚染料和在存在核酸时与核酸存在时的高荧光可用于染色和/或检测核酸。

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