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    • 6. 发明申请
    • GLUTATHIONE-DETECTING FLUORESCENT PROBE
    • 荧光检测荧光探针
    • US20170045525A1
    • 2017-02-16
    • US15116661
    • 2015-02-24
    • THE UNIVERSITY OF TOKYO
    • Yasuteru URANOMako KAMIYAKeitaro UMEZAWAMasafumi YOSHIDA
    • G01N33/68C09K11/07C09B19/00G01N33/58G01N21/64C09B11/24
    • G01N33/68C07F7/081C07F7/0812C09B11/08C09B11/24C09B11/28C09B19/00C09K11/06C09K11/07C09K2211/1007C09K2211/1033C09K2211/1055C09K2211/1088C09K2211/1096G01N21/6428G01N21/6458G01N33/582G01N33/6815G01N2021/6439G01N2021/6441
    • [Problem] To provide the following: a novel fluorescent probe for detecting a compound, such as glutathione, that contains a —SH group; a detection method using said fluorescent probe; and a detection kit containing said probe.[Solution] A fluorescent probe for detecting a compound containing an —SH group, wherein the fluorescent probe comprises a compound represented by formula (I) or a salt thereof (In the formula, X represents Si(Ra)(Rb), Ge(Ra)(Rb), Sn(Ra)(Rb), C(Ra)(Rb), or O (wherein Ra and Rb each independently represent a hydrogen atom or alkyl group); R1 represents a hydrogen atom, or 1-4 identical or different substituents independently selected from the group consisting of a cyano group, alkyl group, carboxyl group, ester group, alkoxy group, amide group, and azide group, each of which may be optionally substituted; R2 represents a hydrogen atom, halogen atom, hydroxyl group, cyano group, or an alkyl group, alkynyl group, alkoxy group, aryl, or heteroaryl, each of which may be optionally substituted; R3 and R4 each independently represent a hydrogen atom or 1-3 identical or different substituents independently selected from the group consisting of a hydroxyl group, halogen atom, or an alkyl group, sulfo group, carboxyl group, ester group, amide group and azide group, each of which may be optionally substituted; R5, R6, R7 and R8 each independently represent a hydrogen atom or alkyl group, wherein, R5 or R6, respectively together with R3, may form a ring structure including the nitrogen atoms bonded thereto, or R7 or R8, respectively together with R4, may form a ring structure including the nitrogen atoms bonded thereto).
    • [解决方案]用于检测含有-SH基团的化合物的荧光探针,其中所述荧光探针包含由式(I)表示的化合物或其盐(在该式中,X表示Si(Ra)(Rb),Ge Ra)(Rb),Sn(Ra)(Rb),C(Ra)(Rb)或O(其中Ra和Rb各自独立地表示氢原子或烷基); R1表示氢原子或1-4 相同或不同的取代基,其独立地选自氰基,烷基,羧基,酯基,烷氧基,酰胺基和叠氮基,其各自可以被任选取代; R 2表示氢原子,卤素原子 ,羟基,氰基或烷基,炔基,烷氧基,芳基或杂芳基,其各自可以被任选取代; R 3和R 4各自独立地表示氢原子或1-3个相同或不同的独立选择的取代基 由羟基,卤素原子或烷基基团组成的组 p,磺基,羧基,酯基,酰胺基和叠氮基,其各自可以任选被取代; R 5,R 6,R 7和R 8各自独立地表示氢原子或烷基,其中,R 5或R 6分别与R 3一起形成包含与其结合的氮原子的环结构,或者R 7或R 8与R 4, 可以形成包括与其结合的氮原子的环结构)。