专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20120053087A1 METHODS FOR IN VITRO JOINING AND COMBINATORIAL ASSEMBLY OF NUCLEIC ACID MOLECULES 审中-公开
标题翻译：核酸分子的体外联合和组合组装方法
公开(公告)号：US20120053087A1
公开(公告)日：2012-03-01
申请号：US13035699
申请日：2011-02-25
申请人： Daniel G. Gibson , Hamilton O. Smith , Clyde A. Hutchison , Lei Young , J. Craig Venter
发明人： Daniel G. Gibson , Hamilton O. Smith , Clyde A. Hutchison , Lei Young , J. Craig Venter
IPC分类号： C12P19/34 , C40B40/06 , C07H21/04 , C12N9/12
CPC分类号： C12P19/34 , C12N15/10 , C12N15/1027 , C12N15/64 , C12N15/66
摘要： The present invention relates to methods of joining two or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule of each pair share a region of sequence identity. The method allows the joining of a large number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest. Kits for performing the method are also disclosed. The methods of joining DNA molecules may be used to generate combinatorial libraries useful to generate, for example, optimal protein expression through codon optimization, gene optimization, and pathway optimization.
摘要翻译：本发明涉及在体外连接两个或多个目的双链（ds）或单链（ss）DNA分子的方法，其中第一DNA分子的远端区域和第二DNA分子的近端区域每对共享序列同一性区域。该方法允许以预定顺序和取向连接大量DNA片段，而不使用限制酶。其可以用于例如合成产生的感兴趣的基因或基因组的亚片段。还公开了用于执行该方法的套件。连接DNA分子的方法可用于产生组合文库，其可用于产生例如通过密码子优化，基因优化和途径优化的最佳蛋白质表达。

2. 发明申请

US20110053273A1 METHODS FOR CLONING AND MANIPULATING GENOMES 有权
标题翻译：克隆和操纵基因的方法
公开(公告)号：US20110053273A1
公开(公告)日：2011-03-03
申请号：US12783489
申请日：2010-05-19
申请人： Gwynedd A. Benders , John I. Glass , Clyde A. Hutchison , Carole Lartigue , Sanjay Vashee , Mikkel A. Algire , Hamilton O. Smith , Charles E. Merryman , Vladimir N. Noskov , Ray-Yuan Chuang , Daniel G. Gibson , J. Craig Venter
发明人： Gwynedd A. Benders , John I. Glass , Clyde A. Hutchison , Carole Lartigue , Sanjay Vashee , Mikkel A. Algire , Hamilton O. Smith , Charles E. Merryman , Vladimir N. Noskov , Ray-Yuan Chuang , Daniel G. Gibson , J. Craig Venter
IPC分类号： C12N15/87 , C12N1/21
CPC分类号： C12N15/81 , C12N15/10 , C12N15/102 , C12N15/1031 , C12N15/1079 , C12N15/1093
摘要： Compositions and methods are disclosed herein for cloning a synthetic or a semi-synthetic donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.
摘要翻译：本文公开了在异源宿主细胞中克隆合成或半合成供体基因组的组合物和方法。在一个实施方案中，可以在宿主细胞内进一步修饰供体基因组。修饰或未修饰的基因组可以进一步从宿主细胞中分离并转移到受体细胞。本文公开的方法可用于在更容易处理的宿主细胞中改变来自难治性供体细胞的供体基因组。

3. 发明申请

US20110053272A1 METHODS FOR CLONING AND MANIPULATING GENOMES 有权
标题翻译：克隆和操纵基因的方法
公开(公告)号：US20110053272A1
公开(公告)日：2011-03-03
申请号：US12718911
申请日：2010-03-05
申请人： Gwynedd A. Benders , John I. Glass , Clyde A. Hutchison, III , Carole Lartigue , Sanjay Vashee , Mikkel A. Algire , Hamilton O. Smith , Charles E. Merryman , Vladimir N. Noskov , Ray-Yuan Chuang , Daniel G. Gibson , J. Craig Venter
发明人： Gwynedd A. Benders , John I. Glass , Clyde A. Hutchison, III , Carole Lartigue , Sanjay Vashee , Mikkel A. Algire , Hamilton O. Smith , Charles E. Merryman , Vladimir N. Noskov , Ray-Yuan Chuang , Daniel G. Gibson , J. Craig Venter
IPC分类号： C12N15/74 , C12N15/82 , C12N15/85 , C12N1/21 , C12N1/15 , C12N1/19 , C12N5/10
CPC分类号： C12N15/905 , C12N15/10 , C12N15/1031 , C12N15/1079 , C12N15/1093 , C12N15/81
摘要： Compositions and methods are disclosed herein for cloning a donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.
摘要翻译：本文公开了用于在异源宿主细胞中克隆供体基因组的组合物和方法。在一个实施方案中，可以在宿主细胞内进一步修饰供体基因组。修饰或未修饰的基因组可以进一步从宿主细胞中分离并转移到受体细胞。本文公开的方法可用于在更容易处理的宿主细胞中改变来自难治性供体细胞的供体基因组。

4. 发明申请

US20100291651A1 RECOMBINANT HYDROGEN-PRODUCING CYANOBACTERIUM AND USES THEREOF 有权
标题翻译：重组氢生产天然产物及其用途
公开(公告)号：US20100291651A1
公开(公告)日：2010-11-18
申请号：US12445929
申请日：2007-10-16
申请人： Qing Xu , Hamilton O. Smith
发明人： Qing Xu , Hamilton O. Smith
IPC分类号： C12P3/00 , C12N1/21 , C07H21/04 , C12N15/63 , C12N9/02
CPC分类号： C12P3/00 , C12N9/0067 , C12N13/00 , Y02P20/134
摘要： A recombinant cyanobacterium comprising an oxygen-tolerant, hydrogen-evolving hydrogenase, kit, and methods of use.
摘要翻译：包含耐氧性，析氢氢化酶，试剂盒和使用方法的重组蓝细菌。

5. 发明申请

US20090275086A1 ASSEMBLY OF LARGE NUCLEIC ACIDS 有权
标题翻译：大型核酸成员大会
公开(公告)号：US20090275086A1
公开(公告)日：2009-11-05
申请号：US12247126
申请日：2008-10-07
申请人： Daniel G. Gibson , Lei Young , John I. Glass , Gwynedd A. Benders , J. Craig Venter , Clyde A. Hutchison, III , Hamilton O. Smith
发明人： Daniel G. Gibson , Lei Young , John I. Glass , Gwynedd A. Benders , J. Craig Venter , Clyde A. Hutchison, III , Hamilton O. Smith
IPC分类号： C12P19/34 , C12N1/19 , C07H21/04
CPC分类号： C12N15/1093 , C12N15/81
摘要： A method to assemble any desired nucleic acid molecule by combining cassettes in vitro to form assemblies which are further combined in vivo, or by assembling large numbers of DNA fragments by recombination in a yeast culture to obtain desired DNA molecules of substantial size is described.
摘要翻译：描述了通过在体外组合盒来组装任何所需核酸分子以形成在体内进一步组合的组装或通过在酵母培养物中通过重组组装大量DNA片段以获得所需的相当大小的DNA分子来组装任何所需核酸分子的方法。

6. 发明授权

US06942975B2 Methods and kits for obtaining DNA end sequence information for large cloned inserts and uses thereof 失效
标题翻译：用于获得大型克隆插入物的DNA末端序列信息的方法和试剂盒及其用途
公开(公告)号：US06942975B2
公开(公告)日：2005-09-13
申请号：US10243738
申请日：2002-09-16
申请人： Robert Holt , Hamilton O. Smith , Cynthia Pfannkoch
发明人： Robert Holt , Hamilton O. Smith , Cynthia Pfannkoch
IPC分类号： C12N15/65 , C12N15/66 , C12Q1/68 , G01N33/53
CPC分类号： C12N15/66 , C12N15/65 , C12Q1/6869
摘要： The present invention provides a method of producing a cloned insert that is representative of the ends of a large segment of DNA from the genome of an organism. Specifically, the present invention provides a clone insert strategy that comprising the steps of: a) isolating a nucleic acid molecule, or portion thereof, wherein said nucleic acid molecule is at least 20 kb; b) ligating said nucleic acid molecule into a vector molecule; c) cutting said nucleic acid molecule with a restriction endonuclease, wherein said restriction endonuclease cuts said isolated nucleic acid molecule of step (a) in at least two sites but does not cut said vector DNA, d) ligating said cut nucleic acid molecule; e) transforming a host cell with said ligated nucleic acid molecule to propagate said molecule; f) determining the sequence of the ends of the nucleic acid insert and the sequence from the restriction endonculease site.
摘要翻译：本发明提供了一种克隆插入物的方法，该插入物代表来自生物体的基因组DNA的大片段的末端。具体地说，本发明提供克隆插入策略，其包括以下步骤：a）分离其中所述核酸分子至少为20kb的核酸分子或其部分; b）将所述核酸分子连接到载体分子中; c）用限制性内切核酸酶切割所述核酸分子，其中所述限制性内切核酸酶在至少两个位点切割步骤（a）的所述分离的核酸分子，但不切割所述载体DNA，d）连接所述切割的核酸分子; e）用所述连接的核酸分子转化宿主细胞以繁殖所述分子; f）确定核酸插入物的末端序列和来自限制性内切酶位点的序列。

7. 发明授权

US06506581B1 Nucleotide sequence of the Haemophilus influenzae Rd genome, fragments thereof, and uses thereof 失效
标题翻译：流感嗜血杆菌Rd基因组的核苷酸序列，其片段及其用途
公开(公告)号：US06506581B1
公开(公告)日：2003-01-14
申请号：US09557884
申请日：2000-04-25
申请人： Robert D. Fleischmann , Mark D. Adams , Owen White , Hamilton O. Smith , J. Craig Venter
发明人： Robert D. Fleischmann , Mark D. Adams , Owen White , Hamilton O. Smith , J. Craig Venter
IPC分类号： C12N121
CPC分类号： C07K14/285 , Y10S435/851
摘要： The present invention provides the sequencing of the entire genome of Haemophilus influenzae Rd, SEQ ID NO:1. The present invention further provides the sequence information stored on computer readable media, and computer-based systems and methods which facilitate its use. In addition to the entire genomic sequence, the present invention identifies over 1700 protein encoding fragments of the genome and identifies, by position relative to a unique Not I restriction endonuclease site, any regulatory elements which modulate the expression of the protein encoding fragments of the Haemophilus genome.
摘要翻译：本发明提供了流感嗜血杆菌Rd，SEQ ID NO：1的整个基因组的测序。本发明还提供存储在计算机可读介质上的序列信息，以及便于其使用的基于计算机的系统和方法。除了整个基因组序列之外，本发明鉴定了基因组的超过1700个蛋白质编码片段，并且通过相对于独特的Not I限制性内切核酸酶位点的位置鉴定调节嗜血杆菌蛋白编码片段的表达的任何调节元件基因组

8. 发明授权

US06355450B1 Computer readable genomic sequence of Haemophilus influenzae Rd, fragments thereof, and uses thereof 失效
标题翻译：流感嗜血杆菌Rd的计算机可读基因组序列及其用途及其用途
公开(公告)号：US06355450B1
公开(公告)日：2002-03-12
申请号：US08476102
申请日：1995-06-07
申请人： Robert D. Fleischmann , Mark D. Adams , Owen White , Hamilton O. Smith , J. Craig Venter
发明人： Robert D. Fleischmann , Mark D. Adams , Owen White , Hamilton O. Smith , J. Craig Venter
IPC分类号： C12P2106
CPC分类号： C07K14/285 , Y10S435/851
摘要： The present invention provides the sequencing of the entire genome of Haemophilus influenzae Rd, SEQ ID NO: 1. The present invention further provides the sequence information stored on computer readable media, and computer-based systems and methods which facilitate its use. In addition to the entire genomic sequence, the present invention identifies over 1700 protein encoding fragments of the genome and identifies, by position relative to a unique Not I restriction endonuclease site, any regulatory elements which modulate the expression of the protein encoding fragments of the Haemophilus genome.
摘要翻译：本发明提供了流感嗜血杆菌Rd，SEQ ID NO：1的整个基因组的测序。本发明还提供存储在计算机可读介质上的序列信息以及便于其使用的基于计算机的系统和方法。除了整个基因组序列之外，本发明鉴定了基因组的超过1700个蛋白质编码片段，并且通过相对于独特的Not I限制性内切核酸酶位点的位置鉴定调节嗜血杆菌蛋白编码片段的表达的任何调节元件基因组

9. 发明授权

US08435736B2 Method for in vitro recombination 有权
标题翻译：体外重组方法
公开(公告)号：US08435736B2
公开(公告)日：2013-05-07
申请号：US12820861
申请日：2010-06-22
申请人： Daniel Glenn Gibson , Hamilton O. Smith
发明人： Daniel Glenn Gibson , Hamilton O. Smith
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12N9/1252 , C12N15/10 , C12N15/102 , C12N15/1031 , C12N15/64 , C12N15/66 , C12P19/34
摘要： The present invention relates to an in vitro method, using isolated protein reagents, for joining two double-stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest.
摘要翻译：本发明涉及一种使用分离的蛋白质试剂连接两个感兴趣的双链（ds）DNA分子的体外方法，其中第一DNA分子的远端区域和第二DNA分子的近端区域共享一个区域的序列同一性。该方法允许以预定的顺序和取向连接多个DNA片段，而不使用限制酶。其可以用于例如合成产生的感兴趣的基因或基因组的亚片段。

10. 发明申请

US20110269119A1 ENCODING TEXT INTO NUCLEIC ACID SEQUENCES 审中-公开
标题翻译：将文本编码为核酸序列
公开(公告)号：US20110269119A1
公开(公告)日：2011-11-03
申请号：US12916344
申请日：2010-10-29
申请人： Clyde A. Hutchison , Michael G. Montague , Hamilton O. Smith
发明人： Clyde A. Hutchison , Michael G. Montague , Hamilton O. Smith
IPC分类号： C12Q1/68 , C12N1/21 , C12N1/00 , C12N7/01 , G06F17/30 , C12N1/13 , C12N5/10 , C12N1/15 , C07H21/00 , C12N15/63 , C12N1/19
CPC分类号： C12N15/64 , C12Q1/6806 , G06F16/86 , C12Q2563/185
摘要： Methods and apparatus are disclosed herein for encoding human readable text conveying a non-genetic message into nucleic acid sequences with a substantially reduced probability of biological impact and decoding such text from nucleic acid sequences. In one embodiment, each symbol of a symbol set of human readable symbols uniquely maps to a respective codon identifier. Mapping may ensure that each symbol will not map to a codon identifier that generates an amino acid residue which has a single-letter abbreviation that is the equivalent to the respective symbol. Synthetic nucleic acid sequences comprising such human readable text, and recombinant or synthetic cells comprising such sequences are provided, as well as methods of identifying cells, organisms, or samples containing such sequences.
摘要翻译：本文公开的方法和装置用于编码将非遗传信息输送到核酸序列中的人类可读文本，其中生物影响的概率显着降低，并且从核酸序列解码该文本。在一个实施例中，人类可读符号的符号集合的每个符号唯一映射到相应的密码子标识符。映射可以确保每个符号不会映射到生成具有等同于相应符号的单字母缩写的氨基酸残基的密码子标识符。提供了包含这种人类可读文本的合成核酸序列，以及包含此类序列的重组或合成细胞，以及鉴定包含该序列的细胞，生物体或样品的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式