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1. 发明授权

US08718351B2 Systems and methods for volumetric tissue scanning microscopy 有权
标题翻译：体积组织扫描显微镜的系统和方法
公开(公告)号：US08718351B2
公开(公告)日：2014-05-06
申请号：US13726931
申请日：2012-12-26
申请人： Peter T. c. So , Bevin Engelward , Timothy Ragan , Karsten Bahlman , Ki-Hean Kim , Lily Hsu Laiho , Hayden Huang
发明人： Peter T. c. So , Bevin Engelward , Timothy Ragan , Karsten Bahlman , Ki-Hean Kim , Lily Hsu Laiho , Hayden Huang
IPC分类号： G06K9/00
CPC分类号： G06K9/00127 , G01N15/1456 , G01N2001/282 , G01N2015/1006
摘要： In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.
摘要翻译：根据本发明的优选实施例，例如，组织成像的方法包括以下步骤：将组织安装在显微镜的计算机控制的阶段上，确定体积成像参数，将至少两个光子引导到感兴趣的区域扫描组织的一部分上的感兴趣区域，在感兴趣区域的多个体积的组织中对组织的多个层进行成像，将组织的该部分切片并成像第二组在感兴趣区域中的组织的第二多个体积中的组织，由于所述激发光检测组织的荧光图像; 以及处理被收集以产生感兴趣区域的三维图像的三维数据。

2. 发明申请

US20100259605A1 SYSTEMS AND METHODS FOR VOLUMETRIC TISSUE SCANNING MICROSCOPY 有权
标题翻译：体积组织扫描显微镜的系统和方法
公开(公告)号：US20100259605A1
公开(公告)日：2010-10-14
申请号：US12757139
申请日：2010-04-09
申请人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Hean Kim , Lily Hsu Laiho , Hayden Huang
发明人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Hean Kim , Lily Hsu Laiho , Hayden Huang
IPC分类号： H04N7/18
CPC分类号： G06K9/00127 , G01N15/1456 , G01N2001/282 , G01N2015/1006
摘要： In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.
摘要翻译：根据本发明的优选实施例，例如，组织成像的方法包括以下步骤：将组织安装在显微镜的计算机控制的阶段上，确定体积成像参数，将至少两个光子引导到感兴趣的区域扫描组织的一部分上的感兴趣区域，在感兴趣区域的多个体积的组织中对组织的多个层进行成像，将组织的该部分切片并成像第二组在感兴趣区域中的组织的第二多个体积中的组织，由于所述激发光检测组织的荧光图像; 以及处理被收集以产生感兴趣区域的三维图像的三维数据。

3. 发明申请

US20090072171A1 Systems and methods for volumetric tissue scanning microscopy 有权
标题翻译：体积组织扫描显微镜的系统和方法
公开(公告)号：US20090072171A1
公开(公告)日：2009-03-19
申请号：US12152136
申请日：2008-05-12
申请人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Hean Kim , Lily Hsu Laiho , Hayden Huang
发明人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Hean Kim , Lily Hsu Laiho , Hayden Huang
IPC分类号： G01N23/04 , H04N7/18
CPC分类号： G06K9/00127 , G01N15/1456 , G01N2001/282 , G01N2015/1006
摘要： In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.
摘要翻译：根据本发明的优选实施例，例如，组织成像的方法包括以下步骤：将组织安装在显微镜的计算机控制的阶段上，确定体积成像参数，将至少两个光子引导到感兴趣的区域扫描组织的一部分上的感兴趣区域，在感兴趣区域的多个体积的组织中对组织的多个层进行成像，将组织的该部分切片并成像第二组在感兴趣区域中的组织的第二多个体积中的组织，由于所述激发光检测组织的荧光图像; 以及处理被收集以产生感兴趣区域的三维图像的三维数据。

4. 发明授权

US5848474A Saw blade clamping arrangement for a power tool 失效
标题翻译：用于电动工具的锯片夹紧装置
公开(公告)号：US5848474A
公开(公告)日：1998-12-15
申请号：US862862
申请日：1997-05-23
申请人： Jodi Fortney , Hayden Huang , Kevan Kudo
发明人： Jodi Fortney , Hayden Huang , Kevan Kudo
IPC分类号： B23D51/10 , B27B19/02
CPC分类号： B23D51/10 , Y10T279/17923
摘要： A saw blade clamping arrangement particularly for power reciprocating saws and similar tools having a reciprocating drive shaft connected to a cutting member such as a saw blade includes a clamping member movable relative to the drive shaft between a clamped position and a release position. A threaded fastener passes through an aperture formed in a portion of the clamping member and functions to move the clamping member to its clamped position upon selective rotation. A sleeve surrounds the threaded fastener for rotation therewith. When the sleeve is biased against the clamping member through the force of a biasing member, opposing surfaces prevent rotation of the sleeve.
摘要翻译：特别是用于动力往复锯的锯片夹持装置和具有连接到诸如锯片的切割构件的往复式驱动轴的类似工具包括可在夹紧位置和释放位置之间相对于驱动轴移动的夹紧构件。螺纹紧固件穿过形成在夹紧构件的一部分中的孔，并且用于在选择性旋转时将夹紧构件移动到其夹紧位置。套筒环绕着螺纹紧固件与其一起旋转。当套筒通过偏置构件的力而抵靠夹紧构件偏置时，相对表面防止套筒旋转。

5. 发明授权

US07372985B2 Systems and methods for volumetric tissue scanning microscopy 有权
标题翻译：体积组织扫描显微镜的系统和方法
公开(公告)号：US07372985B2
公开(公告)日：2008-05-13
申请号：US10642447
申请日：2003-08-15
申请人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Hean Kim , Lily Hsu , Hayden Huang
发明人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Hean Kim , Lily Hsu , Hayden Huang
IPC分类号： G06K9/00
CPC分类号： G06K9/00127 , G01N15/1456 , G01N2001/282 , G01N2015/1006
摘要： In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.
摘要翻译：根据本发明的优选实施例，例如，组织成像的方法包括以下步骤：将组织安装在显微镜的计算机控制的阶段上，确定体积成像参数，将至少两个光子引导到感兴趣的区域扫描组织的一部分上的感兴趣区域，在感兴趣区域的多个体积的组织中对组织的多个层进行成像，将组织的该部分切片并成像第二组在感兴趣区域中的组织的第二多个体积中的组织，由于所述激发光检测组织的荧光图像; 以及处理被收集以产生感兴趣区域的三维图像的三维数据。

6. 发明申请

US20050036667A1 Systems and methods for volumetric tissue scanning microscopy 有权
标题翻译：体积组织扫描显微镜的系统和方法
公开(公告)号：US20050036667A1
公开(公告)日：2005-02-17
申请号：US10642447
申请日：2003-08-15
申请人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Kim , Lily Hsu , Hayden Huang
发明人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Kim , Lily Hsu , Hayden Huang
IPC分类号： G06K9/00
CPC分类号： G06K9/00127 , G01N15/1456 , G01N2001/282 , G01N2015/1006
摘要： In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.
摘要翻译：根据本发明的优选实施例，例如，用于成像组织的方法包括以下步骤：将组织安装在显微镜的计算机控制的阶段上，确定体积成像参数，将至少两个光子引导到感兴趣的区域扫描组织的一部分上的感兴趣区域，在感兴趣区域的多个体积的组织中对组织的多个层进行成像，将组织的该部分切片并成像第二组在感兴趣区域中的组织的第二多个体积中的组织，由于所述激发光检测组织的荧光图像; 以及处理被收集以产生感兴趣区域的三维图像的三维数据。

7. 发明申请

US20140355862A1 SYSTEMS AND METHODS FOR VOLUMETRIC TISSUE SCANNING MICROSCOPY 有权
标题翻译：体积组织扫描显微镜的系统和方法
公开(公告)号：US20140355862A1
公开(公告)日：2014-12-04
申请号：US14270126
申请日：2014-05-05
申请人： Peter T.C. So , Bevin Engelward , Timothy Ragan , Karsten Bahlman , Ki-Hean Kim , Lily Hsu Laiho , Hayden Huang
发明人： Peter T.C. So , Bevin Engelward , Timothy Ragan , Karsten Bahlman , Ki-Hean Kim , Lily Hsu Laiho , Hayden Huang
IPC分类号： G06K9/00
CPC分类号： G06K9/00127 , G01N15/1456 , G01N2001/282 , G01N2015/1006
摘要： In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.
摘要翻译：根据本发明的优选实施例，例如，组织成像的方法包括以下步骤：将组织安装在显微镜的计算机控制的阶段上，确定体积成像参数，将至少两个光子引导到感兴趣的区域扫描组织的一部分上的感兴趣区域，在感兴趣区域的多个体积的组织中对组织的多个层进行成像，将组织的该部分切片并成像第二组在感兴趣区域中的组织的第二多个体积中的组织，由于所述激发光检测组织的荧光图像; 以及处理被收集以产生感兴趣区域的三维图像的三维数据。

8. 发明申请

US20130142413A1 SYSTEMS AND METHODS FOR VOLUMETRIC TISSUE SCANNING MICROSCOPY 有权
标题翻译：体积组织扫描显微镜的系统和方法
公开(公告)号：US20130142413A1
公开(公告)日：2013-06-06
申请号：US13726931
申请日：2012-12-26
申请人： Peter T.c. So , Bevin Engelward , Timothy Ragan , Karsten Bahlman , Ki-Hean Kim , Lily Hsu Laiho , Hayden Huang
发明人： Peter T.c. So , Bevin Engelward , Timothy Ragan , Karsten Bahlman , Ki-Hean Kim , Lily Hsu Laiho , Hayden Huang
IPC分类号： G06K9/00
CPC分类号： G06K9/00127 , G01N15/1456 , G01N2001/282 , G01N2015/1006
摘要： In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.
摘要翻译：根据本发明的优选实施例，例如，组织成像的方法包括以下步骤：将组织安装在显微镜的计算机控制的阶段上，确定体积成像参数，将至少两个光子引导到感兴趣的区域扫描组织的一部分上的感兴趣区域，在感兴趣区域的多个体积的组织中对组织的多个层进行成像，将组织的该部分切片并成像第二组在感兴趣区域中的组织的第二多个体积中的组织，由于所述激发光检测组织的荧光图像; 以及处理被收集以产生感兴趣区域的三维图像的三维数据。

9. 发明授权

US08351675B2 Systems and methods for volumetric tissue scanning microscopy 有权
公开(公告)号：US08351675B2
公开(公告)日：2013-01-08
申请号：US12757139
申请日：2010-04-09
申请人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Hean Kim , Lily Hsu-Laiho , Hayden Huang
发明人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Hean Kim , Lily Hsu-Laiho , Hayden Huang
IPC分类号： G06K9/00
CPC分类号： G06K9/00127 , G01N15/1456 , G01N2001/282 , G01N2015/1006
摘要： In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.

10. 发明授权

US07724937B2 Systems and methods for volumetric tissue scanning microscopy 有权
标题翻译：体积组织扫描显微镜的系统和方法
公开(公告)号：US07724937B2
公开(公告)日：2010-05-25
申请号：US12152136
申请日：2008-05-12
申请人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Hean Kim , Lily Hsu Laiho , Hayden Huang
发明人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Hean Kim , Lily Hsu Laiho , Hayden Huang
IPC分类号： G06K9/00
CPC分类号： G06K9/00127 , G01N15/1456 , G01N2001/282 , G01N2015/1006
摘要： In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.
摘要翻译：根据本发明的优选实施例，例如，组织成像的方法包括以下步骤：将组织安装在显微镜的计算机控制的阶段上，确定体积成像参数，将至少两个光子引导到感兴趣的区域扫描组织的一部分上的感兴趣区域，在感兴趣区域的多个体积的组织中对组织的多个层进行成像，将组织的该部分切片并成像第二组在感兴趣区域中的组织的第二多个体积中的组织，由于所述激发光检测组织的荧光图像; 以及处理被收集以产生感兴趣区域的三维图像的三维数据。

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