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1. 发明申请

US20090072171A1 Systems and methods for volumetric tissue scanning microscopy 有权
标题翻译：体积组织扫描显微镜的系统和方法
公开(公告)号：US20090072171A1
公开(公告)日：2009-03-19
申请号：US12152136
申请日：2008-05-12
申请人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Hean Kim , Lily Hsu Laiho , Hayden Huang
发明人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Hean Kim , Lily Hsu Laiho , Hayden Huang
IPC分类号： G01N23/04 , H04N7/18
CPC分类号： G06K9/00127 , G01N15/1456 , G01N2001/282 , G01N2015/1006
摘要： In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.
摘要翻译：根据本发明的优选实施例，例如，组织成像的方法包括以下步骤：将组织安装在显微镜的计算机控制的阶段上，确定体积成像参数，将至少两个光子引导到感兴趣的区域扫描组织的一部分上的感兴趣区域，在感兴趣区域的多个体积的组织中对组织的多个层进行成像，将组织的该部分切片并成像第二组在感兴趣区域中的组织的第二多个体积中的组织，由于所述激发光检测组织的荧光图像; 以及处理被收集以产生感兴趣区域的三维图像的三维数据。

2. 发明申请

US20100259605A1 SYSTEMS AND METHODS FOR VOLUMETRIC TISSUE SCANNING MICROSCOPY 有权
标题翻译：体积组织扫描显微镜的系统和方法
公开(公告)号：US20100259605A1
公开(公告)日：2010-10-14
申请号：US12757139
申请日：2010-04-09
申请人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Hean Kim , Lily Hsu Laiho , Hayden Huang
发明人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Hean Kim , Lily Hsu Laiho , Hayden Huang
IPC分类号： H04N7/18
CPC分类号： G06K9/00127 , G01N15/1456 , G01N2001/282 , G01N2015/1006
摘要： In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.
摘要翻译：根据本发明的优选实施例，例如，组织成像的方法包括以下步骤：将组织安装在显微镜的计算机控制的阶段上，确定体积成像参数，将至少两个光子引导到感兴趣的区域扫描组织的一部分上的感兴趣区域，在感兴趣区域的多个体积的组织中对组织的多个层进行成像，将组织的该部分切片并成像第二组在感兴趣区域中的组织的第二多个体积中的组织，由于所述激发光检测组织的荧光图像; 以及处理被收集以产生感兴趣区域的三维图像的三维数据。

3. 发明授权

US08351675B2 Systems and methods for volumetric tissue scanning microscopy 有权
公开(公告)号：US08351675B2
公开(公告)日：2013-01-08
申请号：US12757139
申请日：2010-04-09
申请人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Hean Kim , Lily Hsu-Laiho , Hayden Huang
发明人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Hean Kim , Lily Hsu-Laiho , Hayden Huang
IPC分类号： G06K9/00
CPC分类号： G06K9/00127 , G01N15/1456 , G01N2001/282 , G01N2015/1006
摘要： In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.

4. 发明授权

US07724937B2 Systems and methods for volumetric tissue scanning microscopy 有权
标题翻译：体积组织扫描显微镜的系统和方法
公开(公告)号：US07724937B2
公开(公告)日：2010-05-25
申请号：US12152136
申请日：2008-05-12
申请人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Hean Kim , Lily Hsu Laiho , Hayden Huang
发明人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Hean Kim , Lily Hsu Laiho , Hayden Huang
IPC分类号： G06K9/00
CPC分类号： G06K9/00127 , G01N15/1456 , G01N2001/282 , G01N2015/1006
摘要： In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.
摘要翻译：根据本发明的优选实施例，例如，组织成像的方法包括以下步骤：将组织安装在显微镜的计算机控制的阶段上，确定体积成像参数，将至少两个光子引导到感兴趣的区域扫描组织的一部分上的感兴趣区域，在感兴趣区域的多个体积的组织中对组织的多个层进行成像，将组织的该部分切片并成像第二组在感兴趣区域中的组织的第二多个体积中的组织，由于所述激发光检测组织的荧光图像; 以及处理被收集以产生感兴趣区域的三维图像的三维数据。

5. 发明授权

US07372985B2 Systems and methods for volumetric tissue scanning microscopy 有权
标题翻译：体积组织扫描显微镜的系统和方法
公开(公告)号：US07372985B2
公开(公告)日：2008-05-13
申请号：US10642447
申请日：2003-08-15
申请人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Hean Kim , Lily Hsu , Hayden Huang
发明人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Hean Kim , Lily Hsu , Hayden Huang
IPC分类号： G06K9/00
CPC分类号： G06K9/00127 , G01N15/1456 , G01N2001/282 , G01N2015/1006
摘要： In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.
摘要翻译：根据本发明的优选实施例，例如，组织成像的方法包括以下步骤：将组织安装在显微镜的计算机控制的阶段上，确定体积成像参数，将至少两个光子引导到感兴趣的区域扫描组织的一部分上的感兴趣区域，在感兴趣区域的多个体积的组织中对组织的多个层进行成像，将组织的该部分切片并成像第二组在感兴趣区域中的组织的第二多个体积中的组织，由于所述激发光检测组织的荧光图像; 以及处理被收集以产生感兴趣区域的三维图像的三维数据。

6. 发明申请

US20140355862A1 SYSTEMS AND METHODS FOR VOLUMETRIC TISSUE SCANNING MICROSCOPY 有权
标题翻译：体积组织扫描显微镜的系统和方法
公开(公告)号：US20140355862A1
公开(公告)日：2014-12-04
申请号：US14270126
申请日：2014-05-05
申请人： Peter T.C. So , Bevin Engelward , Timothy Ragan , Karsten Bahlman , Ki-Hean Kim , Lily Hsu Laiho , Hayden Huang
发明人： Peter T.C. So , Bevin Engelward , Timothy Ragan , Karsten Bahlman , Ki-Hean Kim , Lily Hsu Laiho , Hayden Huang
IPC分类号： G06K9/00
CPC分类号： G06K9/00127 , G01N15/1456 , G01N2001/282 , G01N2015/1006
摘要： In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.
摘要翻译：根据本发明的优选实施例，例如，组织成像的方法包括以下步骤：将组织安装在显微镜的计算机控制的阶段上，确定体积成像参数，将至少两个光子引导到感兴趣的区域扫描组织的一部分上的感兴趣区域，在感兴趣区域的多个体积的组织中对组织的多个层进行成像，将组织的该部分切片并成像第二组在感兴趣区域中的组织的第二多个体积中的组织，由于所述激发光检测组织的荧光图像; 以及处理被收集以产生感兴趣区域的三维图像的三维数据。

7. 发明授权

US08718351B2 Systems and methods for volumetric tissue scanning microscopy 有权
标题翻译：体积组织扫描显微镜的系统和方法
公开(公告)号：US08718351B2
公开(公告)日：2014-05-06
申请号：US13726931
申请日：2012-12-26
申请人： Peter T. c. So , Bevin Engelward , Timothy Ragan , Karsten Bahlman , Ki-Hean Kim , Lily Hsu Laiho , Hayden Huang
发明人： Peter T. c. So , Bevin Engelward , Timothy Ragan , Karsten Bahlman , Ki-Hean Kim , Lily Hsu Laiho , Hayden Huang
IPC分类号： G06K9/00
CPC分类号： G06K9/00127 , G01N15/1456 , G01N2001/282 , G01N2015/1006
摘要： In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.
摘要翻译：根据本发明的优选实施例，例如，组织成像的方法包括以下步骤：将组织安装在显微镜的计算机控制的阶段上，确定体积成像参数，将至少两个光子引导到感兴趣的区域扫描组织的一部分上的感兴趣区域，在感兴趣区域的多个体积的组织中对组织的多个层进行成像，将组织的该部分切片并成像第二组在感兴趣区域中的组织的第二多个体积中的组织，由于所述激发光检测组织的荧光图像; 以及处理被收集以产生感兴趣区域的三维图像的三维数据。

8. 发明申请

US20130142413A1 SYSTEMS AND METHODS FOR VOLUMETRIC TISSUE SCANNING MICROSCOPY 有权
标题翻译：体积组织扫描显微镜的系统和方法
公开(公告)号：US20130142413A1
公开(公告)日：2013-06-06
申请号：US13726931
申请日：2012-12-26
申请人： Peter T.c. So , Bevin Engelward , Timothy Ragan , Karsten Bahlman , Ki-Hean Kim , Lily Hsu Laiho , Hayden Huang
发明人： Peter T.c. So , Bevin Engelward , Timothy Ragan , Karsten Bahlman , Ki-Hean Kim , Lily Hsu Laiho , Hayden Huang
IPC分类号： G06K9/00
CPC分类号： G06K9/00127 , G01N15/1456 , G01N2001/282 , G01N2015/1006
摘要： In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.
摘要翻译：根据本发明的优选实施例，例如，组织成像的方法包括以下步骤：将组织安装在显微镜的计算机控制的阶段上，确定体积成像参数，将至少两个光子引导到感兴趣的区域扫描组织的一部分上的感兴趣区域，在感兴趣区域的多个体积的组织中对组织的多个层进行成像，将组织的该部分切片并成像第二组在感兴趣区域中的组织的第二多个体积中的组织，由于所述激发光检测组织的荧光图像; 以及处理被收集以产生感兴趣区域的三维图像的三维数据。

9. 发明申请

US20050036667A1 Systems and methods for volumetric tissue scanning microscopy 有权
标题翻译：体积组织扫描显微镜的系统和方法
公开(公告)号：US20050036667A1
公开(公告)日：2005-02-17
申请号：US10642447
申请日：2003-08-15
申请人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Kim , Lily Hsu , Hayden Huang
发明人： Peter So , Bevin Engelward , Timothy Ragan , Karsten Bahlmann , Ki Kim , Lily Hsu , Hayden Huang
IPC分类号： G06K9/00
CPC分类号： G06K9/00127 , G01N15/1456 , G01N2001/282 , G01N2015/1006
摘要： In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.
摘要翻译：根据本发明的优选实施例，例如，用于成像组织的方法包括以下步骤：将组织安装在显微镜的计算机控制的阶段上，确定体积成像参数，将至少两个光子引导到感兴趣的区域扫描组织的一部分上的感兴趣区域，在感兴趣区域的多个体积的组织中对组织的多个层进行成像，将组织的该部分切片并成像第二组在感兴趣区域中的组织的第二多个体积中的组织，由于所述激发光检测组织的荧光图像; 以及处理被收集以产生感兴趣区域的三维图像的三维数据。

10. 发明申请

US20070057211A1 Multifocal imaging systems and method 审中-公开
标题翻译：多焦点成像系统及方法
公开(公告)号：US20070057211A1
公开(公告)日：2007-03-15
申请号：US11442702
申请日：2006-05-25
申请人： Karsten Bahlman , Ki-Hean Kim , Timothy Ragan , Peter So
发明人： Karsten Bahlman , Ki-Hean Kim , Timothy Ragan , Peter So
IPC分类号： G03B42/08
CPC分类号： G01N21/6452 , G01N21/6458 , G01N21/6486 , G02B21/002 , G02B21/0032 , G02B21/0076 , G02B21/16
摘要： In the systems and methods of the present invention a multifocal multiphoton imaging system has a signal to noise ratio (SNR) that is reduced by over an order of magnitude at imaging depth equal to twice the mean free path scattering length of the specimen. An MMM system based on an area detector such as a multianode photomultiplier tube (MAPMT) that is optimized for high-speed tissue imaging. The specimen is raster-scanned with an array of excitation light beams. The emission photons from the array of excitation foci are collected simultaneously by a MAPMT and the signals from each anode are detected using high sensitivity, low noise single photon counting circuits. An image is formed by the temporal encoding of the integrated signal with a raster scanning pattern. A deconvolution procedure taking account of the spatial distribution and the raster temporal encoding of collected photons can be used to improve decay coefficient. We demonstrate MAPMT-based MMM can provide significantly better contrast than CCD-based existing systems.
摘要翻译：在本发明的系统和方法中，多焦点多光子成像系统具有在成像深度等于样本的平均自由路径散射长度的两倍的信噪比（SNR）下降一个数量级以上。基于区域检测器的MMM系统，例如针对高速组织成像而优化的多节点光电倍增管（MAPMT）。样品用激发光束阵列进行光栅扫描。来自激发焦点阵列的发射光子由MAPMT同时收集，并且使用高灵敏度，低噪声单光子计数电路检测来自每个阳极的信号。通过具有光栅扫描图案的积分信号的时间编码形成图像。考虑到收集的光子的空间分布和光栅时间编码的去卷积过程可以用来改善衰变系数。我们演示基于MAPMT的MMM可以提供比基于CCD的现有系统更好的对比度。

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