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    • 10. 发明申请
    • Method for Identification of Enzymes
    • 酶的鉴定方法
    • US20070264632A1
    • 2007-11-15
    • US10581975
    • 2004-12-08
    • Jack NewmanNeil RenningerVincent MartinJay KeaslingKeith Reiling
    • Jack NewmanNeil RenningerVincent MartinJay KeaslingKeith Reiling
    • G01N33/573C12Q1/68
    • C12Q1/00
    • The present invention provides a method of identifying an enzyme, the method generally involving contacting a sample containing an enzyme with a selected enzyme substrate, where the contacting provides for covalent binding of the substrate to an amino acid of the enzyme to form a covalently modified enzyme; and determining the amino acid sequence of at least a portion of the covalently modified enzyme, using any available peptide sequencing technology, such as tandem mass spectrometry. The present invention further provides methods of identifying a nucleic acid encoding an enzyme, the methods generally involving identifying an enzyme; and, based on the amino acid sequence of at least a portion of the enzyme, designing nucleic acid probes or primers that hybridize to the nucleic acid encoding the enzyme.
    • 本发明提供了一种鉴定酶的方法,该方法通常涉及将含有酶的样品与选定的酶底物接触,其中接触提供底物与酶的氨基酸的共价结合以形成共价修饰的酶 ; 以及使用任何可用的肽测序技术(例如串联质谱法)确定共价修饰的酶的至少一部分的氨基酸序列。 本发明还提供鉴定编码酶的核酸的方法,所述方法通常涉及鉴定酶; 并且基于至少部分酶的氨基酸序列,设计与编码该酶的核酸杂交的核酸探针或引物。