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1. 发明申请

US20130252295A1 Production of Isoprenoids 有权
标题翻译：异戊二烯的生产
公开(公告)号：US20130252295A1
公开(公告)日：2013-09-26
申请号：US13848045
申请日：2013-03-20
申请人： Neil Stephen Renninger , Jack Newman , Keith Kinkead Reiling , Rika Regentin , Christopher John Paddon
发明人： Neil Stephen Renninger , Jack Newman , Keith Kinkead Reiling , Rika Regentin , Christopher John Paddon
IPC分类号： C12P5/00 , C12P7/04 , C12P7/02 , C12P5/02
CPC分类号： C12P7/00 , C12N15/52 , C12P5/007 , C12P5/026 , C12P7/02 , C12P7/04
摘要： Methods for producing an isoprenoid are provided. A plurality of bacterial or fungal host cells is obtained. These cells comprise a heterologous nucleic acid encoding one or more enzymes of a mevalonate pathway for making isopentenyl pyrophosphate. Expression of the one or more enzymes is under control of at least one heterologous transcriptional regulator. The mevalonate pathway comprises (i) an enzyme that condenses acetoacetyl-CoA with acetyl-CoA to form HMG-CoA, (ii) an enzyme that converts HMG-CoA to mevalonate, (iii) an enzyme that phosphorylates mevalonate to mevalonate 5-phosphate, (iv) an enzyme that converts mevalonate 5-phosphate to mevalonate 5-pyrophosphate, and (v) an enzyme that converts mevalonate 5-pyrophosphate to isopentenyl pyrophosphate. The host cells are cultured in a medium under conditions that are suboptimal as compared to conditions. Temperature is maintained at a level below that which would provide for a maximum specific growth rate for the host cells.
摘要翻译：提供了制备类异戊二烯的方法。获得多个细菌或真菌宿主细胞。这些细胞包含编码一种或多种用于制备异戊烯基焦磷酸的甲羟戊酸途径的酶的异源核酸。一种或多种酶的表达受至少一种异源转录调节子的控制。甲羟戊酸途径包括（i）使乙酰乙酰辅酶A与乙酰辅酶A缩合以形成HMG-CoA的酶，（ii）将HMG-CoA转化成甲羟戊酸的酶，（iii）将甲羟戊酸磷酸化成甲羟戊酸磷酸酯5-磷酸的酶，（iv）将甲羟戊酸5-磷酸转化为甲基羟基-5-焦磷酸的酶，和（v）将5-焦磷酸甲羟戊酸转化为异戊烯基焦磷酸的酶。宿主细胞在与条件相比不是最佳的条件下在培养基中培养。温度保持在低于为宿主细胞提供最大比生长速率的水平。

2. 发明申请

US20110287476A1 PRODUCTION OF ISOPRENOIDS 审中-公开
标题翻译：异丙酚的生产
公开(公告)号：US20110287476A1
公开(公告)日：2011-11-24
申请号：US12638771
申请日：2009-12-15
申请人： Neil Stephen Renninger , Jack Newman , Keith Kinkead Reiling , Rika Regentin , Christopher John Paddon
发明人： Neil Stephen Renninger , Jack Newman , Keith Kinkead Reiling , Rika Regentin , Christopher John Paddon
IPC分类号： C12P1/00 , C12P15/00 , C12P1/02 , C12P7/04 , C12P1/04 , C12P5/00 , C12P23/00
CPC分类号： C12P7/00 , C12N15/52 , C12P5/007 , C12P5/026 , C12P7/02 , C12P7/04
摘要： The present invention provides methods for a robust production of isoprenoids via one or more biosynthetic pathways. The invention also provides nucleic acids, enzymes, expression vectors, and genetically modified host cells for carrying out the subject methods. The invention also provides fermentation methods for high productivity of isoprenoids from genetically modified host cells.
摘要翻译：本发明提供通过一种或多种生物合成途径强力生产类异戊二烯的方法。本发明还提供用于实施本发明方法的核酸，酶，表达载体和遗传修饰的宿主细胞。本发明还提供了从遗传修饰的宿主细胞中产生高生产力的发酵方法。

3. 发明授权

US07659097B2 Production of isoprenoids 有权
标题翻译：生产类异戊二烯
公开(公告)号：US07659097B2
公开(公告)日：2010-02-09
申请号：US11754235
申请日：2007-05-25
申请人： Neil Stephen Renninger , Jack Newman , Keith Kinkead Reiling , Rika Regentin , Christopher John Paddon
发明人： Neil Stephen Renninger , Jack Newman , Keith Kinkead Reiling , Rika Regentin , Christopher John Paddon
IPC分类号： C12P7/04 , C12P5/00 , C12P23/00 , C12N9/02 , C12N15/82 , C12N15/64 , C12N1/20
CPC分类号： C12P7/00 , C12N15/52 , C12P5/007 , C12P5/026 , C12P7/02 , C12P7/04
摘要： The present invention provides methods for a robust production of isoprenoids via one or more biosynthetic pathways. The invention also provides nucleic acids, enzymes, expression vectors, and genetically modified host cells for carrying out the subject methods. The invention also provides fermentation methods for high productivity of isoprenoids from genetically modified host cells.
摘要翻译：本发明提供通过一种或多种生物合成途径强力生产类异戊二烯的方法。本发明还提供用于实施本发明方法的核酸，酶，表达载体和遗传修饰的宿主细胞。本发明还提供了从遗传修饰的宿主细胞中产生高生产力的发酵方法。

4. 发明申请

US20080274523A1 PRODUCTION OF ISOPRENOIDS 有权
标题翻译：异丙酚的生产
公开(公告)号：US20080274523A1
公开(公告)日：2008-11-06
申请号：US11754235
申请日：2007-05-25
申请人： Neil Stephen Renninger , Jack Newman , Keith Kinkead Rolling , Rika Regentin , Christopher John Paddon
发明人： Neil Stephen Renninger , Jack Newman , Keith Kinkead Rolling , Rika Regentin , Christopher John Paddon
IPC分类号： C12P5/00 , C12P5/02 , C12P7/04 , C12P7/02
CPC分类号： C12P7/00 , C12N15/52 , C12P5/007 , C12P5/026 , C12P7/02 , C12P7/04
摘要： The present invention provides methods for a robust production of isoprenoids via one or more biosynthetic pathways. The invention also provides nucleic acids, enzymes, expression vectors, and genetically modified host cells for carrying out the subject methods. The invention also provides fermentation methods for high productivity of isoprenoids from genetically modified host cells.
摘要翻译：本发明提供通过一种或多种生物合成途径强力生产类异戊二烯的方法。本发明还提供用于实施本发明方法的核酸，酶，表达载体和遗传修饰的宿主细胞。本发明还提供了从遗传修饰的宿主细胞中产生高生产力的发酵方法。

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