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1. 发明授权

US08703423B2 Method of detecting a functional filamentous fungus, method of evaluating a product containing a functional filamentous fungus, and primer pair 失效
标题翻译：检测功能性丝状真菌的方法，评价含有功能性丝状真菌的产品的方法和引物对
公开(公告)号：US08703423B2
公开(公告)日：2014-04-22
申请号：US13060000
申请日：2009-08-20
申请人： Kiyohiko Nakasaki
发明人： Kiyohiko Nakasaki
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6895
摘要： The present invention provides a method of detecting a functional filamentous fungus, wherein the functional filamentous fungus, Coprinellus curtus, in a sample is detected using at least one polynucleotide selected from the group consisting of polynucleotides having a nucleotide sequence shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 4 and polynucleotides having a nucleotide sequence complementary thereto, and polynucleotides having a nucleotide sequence substantially homologous to these sequences; and a method of evaluating a product containing a functional filamentous fungus wherein the existence or concentration of the functional filamentous fungus, Coprinellus curtus, in a product containing a functional filamentous fungus is evaluated.
摘要翻译：本发明提供一种检测功能性丝状真菌的方法，其中使用至少一种选自以下的多核苷酸检测样品中的功能性丝状真菌Coprinellus curtus，所述多核苷酸选自具有SEQ ID NO：1所示核苷酸序列的多核苷酸，SEQ ID NO：2，SEQ ID NO：3或SEQ ID NO：4和具有与其互补的核苷酸序列的多核苷酸，以及具有与这些序列基本同源的核苷酸序列的多核苷酸; 以及评价含有功能性丝状真菌的产品的方法，其中评估了含有功能性丝状真菌的产品中功能性丝状真菌Coprinellus curtus的存在或浓度。

2. 发明授权

US08445252B2 Method for producing functional compost, functional compost and compost for proliferation of filamentous fungus 失效
标题翻译：生产功能堆肥，功能性堆肥和堆肥用于丝状真菌增殖的方法
公开(公告)号：US08445252B2
公开(公告)日：2013-05-21
申请号：US12602463
申请日：2008-06-02
申请人： Kiyohiko Nakasaki , Nobuaki Suzuki
发明人： Kiyohiko Nakasaki , Nobuaki Suzuki
IPC分类号： C12P1/00 , C12N1/00
CPC分类号： C05F11/08 , A01G18/20 , C05F17/0036 , C05G3/02 , C12N1/14 , C12N1/20 , C12P39/00 , C12R1/01 , Y02P20/145 , Y02W30/43
摘要： The method for producing functional compost according to the invention includes: inoculating a filamentous fungus with a function, such as the Coprinus curtus GM-21 strain (NITE BP-37) with a plant disease control function, into compost in an bacterial-activity-restricted state which is, for example, at least one state selected from the group consisting of a nutrient-restricted state, a pH-restricted state and a water content-restricted state; and cultivating the filamentous fungus in the compost to allow its selective proliferation. Further, the invention provides functional compost obtained by the above production method, as well as compost for proliferating a filamentous fungus in a bacterial-activity-restricted state.
摘要翻译：根据本发明的功能堆肥的制备方法包括：将具有植物病害防治功能的具有功能的丝状真菌（例如鬼伞属GM-21菌株（NITE BP-37））接种到细菌活性 - 限制状态是例如从营养受限状态，pH限制状态和含水量限制状态中选择的至少一种状态; 并在堆肥中培养丝状真菌以允许其选择性增殖。此外，本发明提供了通过上述制备方法获得的功能性堆肥，以及用于增殖细菌活性限制状态的丝状真菌的堆肥。

3. 发明申请

US20110177519A1 Method for Detecting Functional Mold, Method for Evaluating Functional Mold-Containing Product and Primer Pair 失效
标题翻译：检测功能模具的方法，评估功能性模具产品和引物对的方法
公开(公告)号：US20110177519A1
公开(公告)日：2011-07-21
申请号：US13060000
申请日：2009-08-20
申请人： Kiyohiko Nakasaki
发明人： Kiyohiko Nakasaki
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6895
摘要： The present invention provides a method of detecting a functional filamentous fungus, wherein the functional filamentous fungus, Coprinellus curtus, in a sample is detected using at least one polynucleotide selected from the group consisting of polynucleotides having a nucleotide sequence shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 4 and polynucleotides having a nucleotide sequence complementary thereto, and polynucleotides having a nucleotide sequence substantially homologous to these sequences; and a method of evaluating a product containing a functional filamentous fungus wherein the existence or concentration of the functional filamentous fungus, Coprinellus curtus, in a product containing a functional filamentous fungus is evaluated.
摘要翻译：本发明提供一种检测功能性丝状真菌的方法，其中使用至少一种选自以下的多核苷酸检测样品中的功能性丝状真菌Coprinellus curtus，所述多核苷酸选自具有SEQ ID NO：1所示核苷酸序列的多核苷酸，SEQ ID NO：2，SEQ ID NO：3或SEQ ID NO：4和具有与其互补的核苷酸序列的多核苷酸，以及具有与这些序列基本同源的核苷酸序列的多核苷酸; 以及评价含有功能性丝状真菌的产品的方法，其中评估了含有功能性丝状真菌的产品中功能性丝状真菌Coprinellus curtus的存在或浓度。

4. 发明申请

US20100212384A1 Method for Producing Functional Compost, Functional Compost and Compost for Proliferation of Filamentous Fungus 失效
标题翻译：生产功能性堆肥，功能性堆肥和堆肥菌丝菌真菌增殖的方法
公开(公告)号：US20100212384A1
公开(公告)日：2010-08-26
申请号：US12602463
申请日：2008-06-02
申请人： Kiyohiko Nakasaki , Nobuaki Suzuki
发明人： Kiyohiko Nakasaki , Nobuaki Suzuki
IPC分类号： C05F11/08
CPC分类号： C05F11/08 , A01G18/20 , C05F17/0036 , C05G3/02 , C12N1/14 , C12N1/20 , C12P39/00 , C12R1/01 , Y02P20/145 , Y02W30/43
摘要： The method for producing functional compost according to the invention includes: inoculating a filamentous fungus with a function, such as the Coprinus curtus GM-21 strain (NITE BP-37) with a plant disease control function, into compost in an bacterial-activity-restricted state which is, for example, at least one state selected from the group consisting of a nutrient-restricted state, a pH-restricted state and a water content-restricted state; and cultivating the filamentous fungus in the compost to allow its selective proliferation. Further, the invention provides functional compost obtained by the above production method, as well as compost for proliferating a filamentous fungus in a bacterial-activity-restricted state.
摘要翻译：根据本发明的功能堆肥的制备方法包括：将具有植物病害防治功能的具有功能的丝状真菌（例如鬼伞属GM-21菌株（NITE BP-37））接种到细菌活性 - 限制状态是例如从营养受限状态，pH限制状态和含水量限制状态中选择的至少一种状态; 并在堆肥中培养丝状真菌以允许其选择性增殖。此外，本发明提供了通过上述制备方法获得的功能性堆肥，以及用于增殖细菌活性限制状态的丝状真菌的堆肥。

5. 发明申请

US20080248058A1 Plant Disease Control Agent And Method For Controlling Plant Disease 审中-公开
标题翻译：植物病害控制剂及控制植物病害的方法
公开(公告)号：US20080248058A1
公开(公告)日：2008-10-09
申请号：US11815743
申请日：2006-02-08
申请人： Kiyohiko Nakasaki , Miyuki Saito
发明人： Kiyohiko Nakasaki , Miyuki Saito
IPC分类号： A01N65/00 , A01H15/00 , A01P3/00
CPC分类号： A01H15/00 , A01N63/04
摘要： The present invention provides a plant disease control agent which is stable and safe in terms of leaving no residue and a method for controlling plant diseases using the same. The plant disease control agent comprises a fragment of an inky cap mushroom, and plant diseases are controlled by using the plant disease control agent. The plant disease control agent may be a suspension comprising the fragment of the inky cap mushroom or a solid material composed of the suspension and a carrier to which the suspension is absorbed. The inky cap mushroom is preferably Coprinus curtus or the like, especially preferably Coprinus curtus GM-21 (NITE BP-37).
摘要翻译：本发明提供一种植物病害控制剂，其在不留下残留物方面是稳定和安全的，以及使用该植物病害防除剂的植物病害防治方法。植物病害控制剂包括墨水蘑菇的片段，并且通过使用植物病害防治剂控制植物病害。植物病害防治剂可以是包含墨水帽蘑菇的片段的悬浮液或由悬浮液和悬浮液吸收的载体组成的固体材料。油墨帽蘑菇优选是鬼伞属（Coprinus curtus）等，特别优选鬼伞属GM-21（NITE BP-37）。

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