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    • 2. 发明授权
    • Alteration of plant morphology by control of profilin expression
    • 通过控制profilin表达改变植物形态
    • US06344601B1
    • 2002-02-05
    • US09061897
    • 1998-04-17
    • Nam-Hai ChuaSrinivasan RamachandranHans Erik Molager Christensen
    • Nam-Hai ChuaSrinivasan RamachandranHans Erik Molager Christensen
    • C12N1582
    • C12N15/827C12N15/8261Y02A40/146
    • Plant growth habit is altered by causing either under-expression or over-expression of profilin in a plant cell. Under-expression of profilin can be achieved by transforming a plant or plant cell with a gene expressing an antisense mRNA complementary to the mRNA transcribed by the coding sequence of a profilin gene and expressing the gene in the plant or plant cell such that the antisense mRNA inhibits the production of the profilin in the plant or plant cell. Under-expression of profilins in plants can lead to such alterations in growth habit as a dwarf phenotype, a reduced root and root hair system, and delay in the onset of flowering. Over-expression of profilin can be achieved by transforming a plant or plant cell with a gene capable of expressing a profilin in the plant or plant cell and causing the transformed gene to be expressed in the plant or plant cell. Over-expression of profilin in a plant can lead to such alterations in growth habit as a tall phenotype, an expansion of the root and root hair system, expansion of leaf surface area and accelerating the onset of flowering.
    • 通过在植物细胞中引起profilin的低表达或过度表达来改变植物生长习性。 profilin的低表达可以通过用表达与由profilin基因的编码序列转录的mRNA互补的反义mRNA的基因转化植物或植物细胞并在植物或植物细胞中表达该基因来实现,使得反义mRNA 抑制植物细胞或植物细胞中profilin的产生。 植物中profilins的低表达可导致生长习性如矮矮化表型,根系和根系减少延迟以及开花发生的延迟。 通过用能够在植物或植物细胞中表达profilin的基因转化植物或植物细胞并使转化的基因在植物或植物细胞中表达,可以实现profilin的过表达。 植物中profilin的过度表达可导致生长习性如高表型,根系和根系发育扩张,叶表面积扩大和加速开花的发生。