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1. 发明授权

US06284544B1 Determination of metal ions in solution by photoluminescence anisotropy 失效
标题翻译：通过光致发光各向异性测定溶液中的金属离子
公开(公告)号：US06284544B1
公开(公告)日：2001-09-04
申请号：US09071351
申请日：1998-04-30
申请人： Richard B. Thompson , Daniel Elbaum , Vincent L. Feliccia , David Christianson , Marcia W. Patchan , Zhengfang Ge , Badri P. Maliwal
发明人： Richard B. Thompson , Daniel Elbaum , Vincent L. Feliccia , David Christianson , Marcia W. Patchan , Zhengfang Ge , Badri P. Maliwal
IPC分类号： G01N3320
CPC分类号： G01N33/542 , G01N21/6445 , G01N33/20 , G01N33/582 , G01N2333/988
摘要： Homogeneous fluorescence polarization (anisotropy) assays for detecting and quantifying metal ions in solution, based the metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule, or the binding of a metal ion to a fluorescent labeled macromolecule. The metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule (metallo-macromolecule) effects a measurable change in anisotropy as will the binding of metal ions to a fluorescent labeled macromolecule. Binding of the fluorescent ligand to the unlabeled macromolecule is metal dependent with the change in anisotropy being proportional to the concentration of bound metal ions. No binding of the fluorescent ligand to the macromolecule occurs in the absence of metal ions. Conversely, if the fluorescent label is first conjugated to a metallo-macromolecule and the metallo-macromolecule is subsequently stripped of its metal ion, it may then be used to transduce the binding of metal ions. Transduction is provided wherein the covalently bound fluorescent label exhibits changes in anisotropy proportional to the concentration of bound metal ions. In all methods, the change in anisotropy may be simply related to the metal ion concentration of the test solution.
摘要翻译：基于荧光配体与未标记的大分子的金属依赖性结合，或金属离子与荧光标记的大分子的结合，用于检测和定量溶液中的金属离子的均匀荧光偏振（各向异性）测定。荧光配体与未标记的大分子（金属 - 大分子）的金属依赖性结合对金属离子与荧光标记的大分子的结合将产生各向异性的可测量的变化。荧光配体与未标记的大分子的结合是金属依赖性的，各向异性的变化与结合的金属离子的浓度成比例。在不存在金属离子的情况下，不发生荧光配体与大分子的结合。相反，如果荧光标记首先与金属 - 大分子缀合，并且金属 - 大分子随后被其金属离子剥离，则其可用于转导金属离子的结合。提供转导，其中共价结合的荧光标记表现出与结合的金属离子浓度成比例的各向异性变化。在各种方法中，各向异性的变化可能与测试溶液的金属离子浓度有关。

2. 发明授权

US07842491B2 Determination of metal ions in solution by photoluminescence anisotropy 有权
标题翻译：通过光致发光各向异性测定溶液中的金属离子
公开(公告)号：US07842491B2
公开(公告)日：2010-11-30
申请号：US10982956
申请日：2004-11-08
申请人： Richard B. Thompson , Daniel Elbaum , Vincent L. Feliccia , David Christianson , Marcia W. Patchan , Zhengfang Ge , Badri P. Maliwal
发明人： Richard B. Thompson , Daniel Elbaum , Vincent L. Feliccia , David Christianson , Marcia W. Patchan , Zhengfang Ge , Badri P. Maliwal
IPC分类号： C12Q1/34 , C12Q1/00 , G01N21/00 , G01N33/20
CPC分类号： G01N33/542 , C12N9/88 , G01N21/6445 , Y10S435/975
摘要： The invention relates to compositions and kits for homogeneous fluorescence polarization (anisotropy) assays for detecting and quantifying metal ions in solution. Metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule effects a measurable change in anisotropy as will the binding of metal ions to a fluorescent labeled macromolecule. Binding of the fluorescent ligand to the unlabeled macromolecule is metal dependent with the change in anisotropy being proportional to the concentration of bound metal ions. Conversely, if the fluorescent label is first conjugated to a macromolecule and the macromolecule is subsequently stripped of metal ion, it may then be used to signal binding of metal ions. The covalently bound fluorescent label exhibits changes in anisotropy proportional to the concentration of bound metal ions. Kits comprise a fluorescent molecule and a macromolecule.
摘要翻译：本发明涉及用于检测和定量溶液中的金属离子的均匀荧光偏振（各向异性）测定的组合物和试剂盒。荧光配体与未标记的大分子的金属依赖性结合影响各向异性的可测量的变化，以及金属离子与荧光标记的大分子的结合。荧光配体与未标记的大分子的结合是金属依赖性的，各向异性的变化与结合的金属离子的浓度成比例。相反，如果荧光标记首先与大分子缀合并且随后大分子被金属离子剥离，则可以用它来信号结合金属离子。共价结合的荧光标记表现出与结合金属离子浓度成比例的各向异性变化。试剂盒包含荧光分子和大分子。

3. 发明申请

US20050250170A1 Determination of metal ions in solution by photoluminescence anisotropy 有权
标题翻译：通过光致发光各向异性测定溶液中的金属离子
公开(公告)号：US20050250170A1
公开(公告)日：2005-11-10
申请号：US10982956
申请日：2004-11-08
申请人： Richard Thompson , Daniel Elbaum , Vincent Feliccia , David Christianson , Marcia Patchan , Zhengfang Ge , Badri Maliwal
发明人： Richard Thompson , Daniel Elbaum , Vincent Feliccia , David Christianson , Marcia Patchan , Zhengfang Ge , Badri Maliwal
IPC分类号： C12N9/16 , C12N9/88 , C12Q1/34 , G01N21/64 , G01N33/20 , G01N33/542
CPC分类号： G01N33/542 , C12N9/88 , G01N21/6445 , Y10S435/975
摘要： The invention relates to compositions and kits for homogeneous fluorescence polarization (anisotropy) assays for detecting and quantifying metal ions in solution. Metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule effects a measurable change in anisotropy as will the binding of metal ions to a fluorescent labeled macromolecule. Binding of the fluorescent ligand to the unlabeled macromolecule is metal dependent with the change in anisotropy being proportional to the concentration of bound metal ions. Conversely, if the fluorescent label is first conjugated to a macromolecule and the macromolecule is subsequently stripped of metal ion, it may then be used to signal binding of metal ions. The covalently bound fluorescent label exhibits changes in anisotropy proportional to the concentration of bound metal ions. Kits comprise a fluorescent molecule and a macromolecule.
摘要翻译：本发明涉及用于检测和定量溶液中的金属离子的均匀荧光偏振（各向异性）测定的组合物和试剂盒。荧光配体与未标记的大分子的金属依赖性结合影响各向异性的可测量变化，以及金属离子与荧光标记的大分子的结合。荧光配体与未标记的大分子的结合是金属依赖性的，各向异性的变化与结合的金属离子的浓度成比例。相反，如果首先将荧光标记与大分子缀合并且随后剥离大分子金属离子，则可以用它来信号结合金属离子。共价结合的荧光标记表现出与结合金属离子浓度成比例的各向异性变化。试剂盒包含荧光分子和大分子。

4. 发明授权

US5952236A Enzyme-based fluorescence biosensor for chemical analysis 失效
标题翻译：基于酶的荧光生物传感器进行化学分析
公开(公告)号：US5952236A
公开(公告)日：1999-09-14
申请号：US736904
申请日：1996-10-25
申请人： Richard B. Thompson , Marcia W. Patchan , Zhengfang Ge
发明人： Richard B. Thompson , Marcia W. Patchan , Zhengfang Ge
IPC分类号： G01N31/22 , G01N33/542 , G01N33/84 , G01N33/48
CPC分类号： G01N33/84 , G01N31/22 , G01N33/542
摘要： This invention generally relates to the detection, determination, and quantitation of certain ions and small molecules involving the quenching of a fluorescent label attached to a macromolecule, often due to fluorescence energy transfer to a colored inhibitor or certain metal ions bound to the macromolecule.
摘要翻译：本发明一般涉及某些离子和小分子的检测，测定和定量，这些离子和小分子涉及连接到大分子上的荧光标记物的猝灭，这通常是由于荧光能量转移到着色抑制剂或与大分子结合的某些金属离子。

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