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1. 发明授权

US06284544B1 Determination of metal ions in solution by photoluminescence anisotropy 失效
标题翻译：通过光致发光各向异性测定溶液中的金属离子
公开(公告)号：US06284544B1
公开(公告)日：2001-09-04
申请号：US09071351
申请日：1998-04-30
申请人： Richard B. Thompson , Daniel Elbaum , Vincent L. Feliccia , David Christianson , Marcia W. Patchan , Zhengfang Ge , Badri P. Maliwal
发明人： Richard B. Thompson , Daniel Elbaum , Vincent L. Feliccia , David Christianson , Marcia W. Patchan , Zhengfang Ge , Badri P. Maliwal
IPC分类号： G01N3320
CPC分类号： G01N33/542 , G01N21/6445 , G01N33/20 , G01N33/582 , G01N2333/988
摘要： Homogeneous fluorescence polarization (anisotropy) assays for detecting and quantifying metal ions in solution, based the metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule, or the binding of a metal ion to a fluorescent labeled macromolecule. The metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule (metallo-macromolecule) effects a measurable change in anisotropy as will the binding of metal ions to a fluorescent labeled macromolecule. Binding of the fluorescent ligand to the unlabeled macromolecule is metal dependent with the change in anisotropy being proportional to the concentration of bound metal ions. No binding of the fluorescent ligand to the macromolecule occurs in the absence of metal ions. Conversely, if the fluorescent label is first conjugated to a metallo-macromolecule and the metallo-macromolecule is subsequently stripped of its metal ion, it may then be used to transduce the binding of metal ions. Transduction is provided wherein the covalently bound fluorescent label exhibits changes in anisotropy proportional to the concentration of bound metal ions. In all methods, the change in anisotropy may be simply related to the metal ion concentration of the test solution.
摘要翻译：基于荧光配体与未标记的大分子的金属依赖性结合，或金属离子与荧光标记的大分子的结合，用于检测和定量溶液中的金属离子的均匀荧光偏振（各向异性）测定。荧光配体与未标记的大分子（金属 - 大分子）的金属依赖性结合对金属离子与荧光标记的大分子的结合将产生各向异性的可测量的变化。荧光配体与未标记的大分子的结合是金属依赖性的，各向异性的变化与结合的金属离子的浓度成比例。在不存在金属离子的情况下，不发生荧光配体与大分子的结合。相反，如果荧光标记首先与金属 - 大分子缀合，并且金属 - 大分子随后被其金属离子剥离，则其可用于转导金属离子的结合。提供转导，其中共价结合的荧光标记表现出与结合的金属离子浓度成比例的各向异性变化。在各种方法中，各向异性的变化可能与测试溶液的金属离子浓度有关。

2. 发明授权

US07842491B2 Determination of metal ions in solution by photoluminescence anisotropy 有权
标题翻译：通过光致发光各向异性测定溶液中的金属离子
公开(公告)号：US07842491B2
公开(公告)日：2010-11-30
申请号：US10982956
申请日：2004-11-08
申请人： Richard B. Thompson , Daniel Elbaum , Vincent L. Feliccia , David Christianson , Marcia W. Patchan , Zhengfang Ge , Badri P. Maliwal
发明人： Richard B. Thompson , Daniel Elbaum , Vincent L. Feliccia , David Christianson , Marcia W. Patchan , Zhengfang Ge , Badri P. Maliwal
IPC分类号： C12Q1/34 , C12Q1/00 , G01N21/00 , G01N33/20
CPC分类号： G01N33/542 , C12N9/88 , G01N21/6445 , Y10S435/975
摘要： The invention relates to compositions and kits for homogeneous fluorescence polarization (anisotropy) assays for detecting and quantifying metal ions in solution. Metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule effects a measurable change in anisotropy as will the binding of metal ions to a fluorescent labeled macromolecule. Binding of the fluorescent ligand to the unlabeled macromolecule is metal dependent with the change in anisotropy being proportional to the concentration of bound metal ions. Conversely, if the fluorescent label is first conjugated to a macromolecule and the macromolecule is subsequently stripped of metal ion, it may then be used to signal binding of metal ions. The covalently bound fluorescent label exhibits changes in anisotropy proportional to the concentration of bound metal ions. Kits comprise a fluorescent molecule and a macromolecule.
摘要翻译：本发明涉及用于检测和定量溶液中的金属离子的均匀荧光偏振（各向异性）测定的组合物和试剂盒。荧光配体与未标记的大分子的金属依赖性结合影响各向异性的可测量的变化，以及金属离子与荧光标记的大分子的结合。荧光配体与未标记的大分子的结合是金属依赖性的，各向异性的变化与结合的金属离子的浓度成比例。相反，如果荧光标记首先与大分子缀合并且随后大分子被金属离子剥离，则可以用它来信号结合金属离子。共价结合的荧光标记表现出与结合金属离子浓度成比例的各向异性变化。试剂盒包含荧光分子和大分子。

3. 发明授权

US06197258B1 Photoluminescent sensors of chemical analytes 失效
标题翻译：化学分析物的光致发光传感器
公开(公告)号：US06197258B1
公开(公告)日：2001-03-06
申请号：US09273303
申请日：1999-03-19
申请人： Richard B. Thompson , Vincent L. Feliccia , Badri P. Maliwal , Carol A. Fierke
发明人： Richard B. Thompson , Vincent L. Feliccia , Badri P. Maliwal , Carol A. Fierke
IPC分类号： G01N3320
CPC分类号： G01N33/582 , G01N33/20 , G01N33/542 , G01N2333/988
摘要： The invention described in detail herein relates to the detection, determination, and quantitation of certain ions and small molecules in solution. The invention specifically relates to improvements in the area of photoluminescent sensors for use in a detection scheme involving the alteration of a photoluminescent label or moiety attached to or associated with an analyte binding macromolecule. One may use the changes in photoluminescence lifetime, changes in ratios of photoluminescence intensity or changes in photoluminescence polarization (anisotropy) to determine the analyte. The photoluminescence change measured correlates to the concentration of the ion or molecule in solution.
摘要翻译：本文详细描述的本发明涉及溶液中某些离子和小分子的检测，测定和定量。本发明具体涉及用于检测方案中的光致发光传感器的改进，所述检测方案涉及改变附着于或与分析物结合大分子结合的光致发光标记或部分。可以使用光致发光寿命的变化，光致发光强度比例的变化或光致发光偏振的变化（各向异性）来确定分析物。测量的光致变化与溶液中离子或分子的浓度相关。

4. 发明授权

US5952236A Enzyme-based fluorescence biosensor for chemical analysis 失效
标题翻译：基于酶的荧光生物传感器进行化学分析
公开(公告)号：US5952236A
公开(公告)日：1999-09-14
申请号：US736904
申请日：1996-10-25
申请人： Richard B. Thompson , Marcia W. Patchan , Zhengfang Ge
发明人： Richard B. Thompson , Marcia W. Patchan , Zhengfang Ge
IPC分类号： G01N31/22 , G01N33/542 , G01N33/84 , G01N33/48
CPC分类号： G01N33/84 , G01N31/22 , G01N33/542
摘要： This invention generally relates to the detection, determination, and quantitation of certain ions and small molecules involving the quenching of a fluorescent label attached to a macromolecule, often due to fluorescence energy transfer to a colored inhibitor or certain metal ions bound to the macromolecule.
摘要翻译：本发明一般涉及某些离子和小分子的检测，测定和定量，这些离子和小分子涉及连接到大分子上的荧光标记物的猝灭，这通常是由于荧光能量转移到着色抑制剂或与大分子结合的某些金属离子。

5. 发明授权

US06225127B1 Enzyme-based fluorescence biosensor for chemical analysis 失效
标题翻译：基于酶的荧光生物传感器进行化学分析
公开(公告)号：US06225127B1
公开(公告)日：2001-05-01
申请号：US09270308
申请日：1999-03-15
申请人： Richard B. Thompson , Marcia W. Patchan , Zhenfang Ge
发明人： Richard B. Thompson , Marcia W. Patchan , Zhenfang Ge
IPC分类号： G01N3348
CPC分类号： G01N33/84 , G01N31/22 , G01N33/542
摘要： This invention generally relates to the detection, determination, and quantitation of certain ions and small molecules involving the quenching of a fluorescent label attached to a macromolecule, often due to fluorescence energy transfer to a colored inhibitor or certain metal ions bound to the macromolecule.
摘要翻译：本发明一般涉及某些离子和小分子的检测，测定和定量，这些离子和小分子涉及连接到大分子上的荧光标记物的猝灭，这通常是由于荧光能量转移到着色抑制剂或与大分子结合的某些金属离子。

6. 发明授权

US4457513A Rebound ball game and apparatus therefor 失效
标题翻译：反弹球游戏及其设备
公开(公告)号：US4457513A
公开(公告)日：1984-07-03
申请号：US474167
申请日：1983-03-10
申请人： Richard B. Thompson
发明人： Richard B. Thompson
IPC分类号： A63B61/00
CPC分类号： A63B61/006 , A63B2208/12
摘要： A rebound ball game in which a ball is returned between opposed players within a prescribed court divided by a rebound apparatus having rebound faces and a specified return area, the rebound faces being angled for rebound of the ball toward the respective players, and the return area being located above the rebound faces for defining the appropriate area through which the ball is to be passed between the opposed players, the court having limited trapezoidal playing areas at each side of the rebound apparatus.
摘要翻译：一种反弹球游戏，其中在规定的法院内的相对的玩家之间返回球，由具有反弹面的反弹装置和指定的返回区域返回，弹回面朝向相应的玩家反弹，并且返回区域位于反弹面之上，用于定义球在相对的对手之间通过的适当区域，法院在回弹装置的每一侧具有有限的梯形游戏区域。

7. 发明授权

US09193990B2 Bioluminescent metal ion assay 有权
标题翻译：生物发光金属离子测定
公开(公告)号：US09193990B2
公开(公告)日：2015-11-24
申请号：US14218085
申请日：2014-03-18
申请人： Richard B. Thompson
发明人： Richard B. Thompson , Evgenia Matveeva , Carol Fierke , Leslie Bourne , Graham Franke
IPC分类号： C12Q1/66 , G01N33/542
CPC分类号： C12Q1/66 , G01N33/542 , G01N2333/988
摘要： A method for determining metal ions, both qualitatively and quantitatively, is disclosed. The method utilizes emission from fluorescence resonance energy transfer from a luciferase-carbonic anhydrase conjugate or fusion protein to an acceptor ligand in the presence of metal ion bound to the protein to measure free metal ion concentrations down to picomolar concentration ranges. The method is relatively insensitive to contaminants, and so can be used to measure metal ion concentrations in cells, body fluids or environmental samples without extensive sample preparation.
摘要翻译：本发明公开了一种定性和定量测定金属离子的方法。该方法利用与蛋白质结合的金属离子的存在下从荧光素酶 - 碳酸酐酶偶联物或融合蛋白到受体配体的荧光共振能量转移的发射，以测量低至皮摩尔浓度范围的游离金属离子浓度。该方法对污染物相对不敏感，因此可用于测量细胞，体液或环境样品中的金属离子浓度，而无需广泛的样品制备。

8. 发明授权

US5545517A Selective metal ion detection using a photoluminescent indicator binding to a macromolecule-metal ion complex 失效
标题翻译：使用结合大分子金属离子络合物的光致发光指示剂进行选择性金属离子检测
公开(公告)号：US5545517A
公开(公告)日：1996-08-13
申请号：US213409
申请日：1994-03-15
申请人： Richard B. Thompson , Eric R. Jones
发明人： Richard B. Thompson , Eric R. Jones
IPC分类号： C12Q1/527 , G01N21/64 , G01N31/22 , C12Q1/00 , G01N21/00
CPC分类号： G01N21/643 , C12Q1/527 , G01N31/22 , Y10S435/808 , Y10S436/805
摘要： The present invention is a process and apparatus for metal ion detection. The process of the invention has the steps of (1) disposing, in an analyte medium, a macromolecule suitable for selective complexation with the target metal ion species; (2) disposing, in the analyte medium, an appropriate photoluminescent indicator that will emit in a measurably different manner when bound to the metallomacromolecule complex, compared with its unbound state; (3) exciting the photoluminescent indicator species; and (4) monitoring the emission of the photoluminescent indicator species to detect changes in its emission. The apparatus of the invention has (1) a macromolecule suitable for selective complexation with the target metal ion species disposed in an analyte medium; (2) an appropriate photoluminescent indicator that will emit in a measurably different manner when bound to the metallomacromolecule complex, compared with its unbound state, also disposed in the analyte medium; (3) a source for exciting the photoluminescent indicator species; and (4) a detector for monitoring the emission of the photoluminescent indicator species to detect changes in its emission.
摘要翻译：本发明是用于金属离子检测的方法和装置。本发明的方法具有以下步骤：（1）在分析物介质中设置适于与目标金属离子物质选择性络合的大分子; （2）在分析物介质中，与其未结合状态相比，在与金属大分子复合物结合时以相当不同的方式配置适当的光致发光指示剂; （3）激发光致发光指示物种; 和（4）监测光致发光指示剂物质的发射以检测其发射的变化。本发明的装置具有（1）适用于与分析物介质中配置的靶金属离子种类选择性络合的大分子; （2）当与其未结合状态相比，当与金属大分子复合物结合时，其可以以可测量不同的方式发射，也放置在分析物介质中的合适的光致发光指示剂; （3）激发光致发光指示物种的来源; 和（4）用于监测光致发光指示剂物质的发射以检测其发射变化的检测器。

9. 发明授权

US5455700A Regenerative communication channel extender 失效
标题翻译：再生通信渠道扩展器
公开(公告)号：US5455700A
公开(公告)日：1995-10-03
申请号：US876653
申请日：1992-04-30
申请人： Richard B. Thompson , Scott J. Nicolet
发明人： Richard B. Thompson , Scott J. Nicolet
IPC分类号： H04L1/22 , H04L12/46 , H04L25/20 , H04L29/06 , H04J14/00 , H04B10/02 , H04B10/16
CPC分类号： H04L29/06 , H04L12/46
摘要： A regenerative digital communication channel extender is provided which converts electrical signals in a first communication network into optical signals and then reconverts the optical signals back into electrical signals for application to a second communication network. The conversion is preferably performed using state machines. The communication in the first and second communication networks are preferably in a first protocol, whereas the optical communication is in a second protocol. The state machines convert the communications from the first protocol to the second protocol, and back from the second protocol into the first protocol. In the process, the communication in the first communication network is faithfully regenerated in the second communication network. In addition, a unique phase lock circuit is contemplated which requires only one pulse of an incoming digital communication to synchronize a local clock.
摘要翻译：提供再生数字通信信道扩展器，其将第一通信网络中的电信号转换为光信号，然后将光信号重新转换回电信号以应用于第二通信网络。转换优选使用状态机进行。第一和第二通信网络中的通信优选地处于第一协议中，而光通信处于第二协议。状态机将来自第一协议的通信转换为第二协议，并从第二协议返回到第一协议。在该过程中，在第二通信网络中忠实地再生第一通信网络中的通信。此外，构想了唯一的锁相电路，其仅需要输入数字通信的一个脉冲来同步本地时钟。

10. 发明授权

US08214296B2 Disaggregated secure execution environment 有权
标题翻译：分解的安全执行环境
公开(公告)号：US08214296B2
公开(公告)日：2012-07-03
申请号：US11353675
申请日：2006-02-14
申请人： Alexander Frank , Curt A. Steeb , Isaac P. Ahdout , Richard B. Thompson , Thomas G. Phillips , William J. Westerinen , Zhangwei Xu
发明人： Alexander Frank , Curt A. Steeb , Isaac P. Ahdout , Richard B. Thompson , Thomas G. Phillips , William J. Westerinen , Zhangwei Xu
IPC分类号： G06F21/00
CPC分类号： H04L9/00 , G06F21/55 , G06F21/554 , G06F2221/2135
摘要： An electronic device, such as, a computer, may be adapted for self-monitoring for compliance to an operating policy. The operating policy may specify a pay-per-use or subscription business model and measurements associated with compliant usage. A secure execution environment may measure usage in accordance with the business model as well as monitor and enforce compliance to the operating policy. To increase the difficulty of attacking or otherwise disabling the secure execution environment, elements of the secure execution environment may be distributed. The distribution points may include other functional elements of the computer, such as interface circuits, or may even be remotely located over a network. An implementation method for disaggregating the secure execution environment is also disclosed.
摘要翻译：诸如计算机的电子设备可以适于自我监视以符合操作策略。操作策略可以指定按使用付费或订阅业务模式以及与合规使用相关联的测量。安全执行环境可以根据业务模式来测量使用情况，并监视和实施对操作策略的遵守。为了增加攻击或以其他方式禁用安全执行环境的难度，可以分发安全执行环境的元素。分发点可以包括计算机的其他功能元件，例如接口电路，或者甚至可以远程位于网络上。还公开了用于分解安全执行环境的实现方法。

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