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1. 发明申请

US20140273038A1 BIOLUMINESCENT METAL ION ASSAY 有权
标题翻译：生物发光金属离子测定
公开(公告)号：US20140273038A1
公开(公告)日：2014-09-18
申请号：US14218085
申请日：2014-03-18
申请人： RICHARD B. THOMPSON
发明人： Richard B. Thompson , Evgenia Matveeva , Carol Fierke , Leslie Bourne , Graham Franke
IPC分类号： C12Q1/66
CPC分类号： C12Q1/66 , G01N33/542 , G01N2333/988
摘要： A method for determining metal ions, both qualitatively and quantitatively, is disclosed. The method utilizes emission from fluorescence resonance energy transfer from a luciferase-carbonic anhydrase conjugate or fusion protein to an acceptor ligand in the presence of metal ion bound to the protein to measure free metal ion concentrations down to picomolar concentration ranges. The method is relatively insensitive to contaminants, and so can be used to measure metal ion concentrations in cells, body fluids or environmental samples without extensive sample preparation.
摘要翻译：本发明公开了一种定性和定量测定金属离子的方法。该方法利用与蛋白质结合的金属离子的存在下从荧光素酶 - 碳酸酐酶偶联物或融合蛋白到受体配体的荧光共振能量转移的发射，以测量低至皮摩尔浓度范围的游离金属离子浓度。该方法对污染物相对不敏感，因此可用于测量细胞，体液或环境样品中的金属离子浓度，而无需广泛的样品制备。

2. 发明授权

US08073779B2 Disaggregated secure execution environment 有权
公开(公告)号：US08073779B2
公开(公告)日：2011-12-06
申请号：US11353675
申请日：2006-02-14
申请人： Alexander Frank , Curt A. Steeb , Isaac P. Ahdout , Richard B. Thompson , Thomas G. Phillips , William J. Westerinen , Zhangwei Xu
发明人： Alexander Frank , Curt A. Steeb , Isaac P. Ahdout , Richard B. Thompson , Thomas G. Phillips , William J. Westerinen , Zhangwei Xu
IPC分类号： G06F21/00
摘要： An electronic device, such as, a computer, may be adapted for self-monitoring for compliance to an operating policy. The operating policy may specify a pay-per-use or subscription business model and measurements associated with compliant usage. A secure execution environment may measure usage in accordance with the business model as well as monitor and enforce compliance to the operating policy. To increase the difficulty of attacking or otherwise disabling the secure execution environment, elements of the secure execution environment may be distributed. The distribution points may include other functional elements of the computer, such as interface circuits, or may even be remotely located over a network. An implementation method for disaggregating the secure execution environment is also disclosed.

3. 发明授权

US06284544B1 Determination of metal ions in solution by photoluminescence anisotropy 失效
标题翻译：通过光致发光各向异性测定溶液中的金属离子
公开(公告)号：US06284544B1
公开(公告)日：2001-09-04
申请号：US09071351
申请日：1998-04-30
申请人： Richard B. Thompson , Daniel Elbaum , Vincent L. Feliccia , David Christianson , Marcia W. Patchan , Zhengfang Ge , Badri P. Maliwal
发明人： Richard B. Thompson , Daniel Elbaum , Vincent L. Feliccia , David Christianson , Marcia W. Patchan , Zhengfang Ge , Badri P. Maliwal
IPC分类号： G01N3320
CPC分类号： G01N33/542 , G01N21/6445 , G01N33/20 , G01N33/582 , G01N2333/988
摘要： Homogeneous fluorescence polarization (anisotropy) assays for detecting and quantifying metal ions in solution, based the metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule, or the binding of a metal ion to a fluorescent labeled macromolecule. The metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule (metallo-macromolecule) effects a measurable change in anisotropy as will the binding of metal ions to a fluorescent labeled macromolecule. Binding of the fluorescent ligand to the unlabeled macromolecule is metal dependent with the change in anisotropy being proportional to the concentration of bound metal ions. No binding of the fluorescent ligand to the macromolecule occurs in the absence of metal ions. Conversely, if the fluorescent label is first conjugated to a metallo-macromolecule and the metallo-macromolecule is subsequently stripped of its metal ion, it may then be used to transduce the binding of metal ions. Transduction is provided wherein the covalently bound fluorescent label exhibits changes in anisotropy proportional to the concentration of bound metal ions. In all methods, the change in anisotropy may be simply related to the metal ion concentration of the test solution.
摘要翻译：基于荧光配体与未标记的大分子的金属依赖性结合，或金属离子与荧光标记的大分子的结合，用于检测和定量溶液中的金属离子的均匀荧光偏振（各向异性）测定。荧光配体与未标记的大分子（金属 - 大分子）的金属依赖性结合对金属离子与荧光标记的大分子的结合将产生各向异性的可测量的变化。荧光配体与未标记的大分子的结合是金属依赖性的，各向异性的变化与结合的金属离子的浓度成比例。在不存在金属离子的情况下，不发生荧光配体与大分子的结合。相反，如果荧光标记首先与金属 - 大分子缀合，并且金属 - 大分子随后被其金属离子剥离，则其可用于转导金属离子的结合。提供转导，其中共价结合的荧光标记表现出与结合的金属离子浓度成比例的各向异性变化。在各种方法中，各向异性的变化可能与测试溶液的金属离子浓度有关。

4. 发明授权

US5141312A Fiber optic photoluminescence sensor 失效
标题翻译：光纤光致发光传感器
公开(公告)号：US5141312A
公开(公告)日：1992-08-25
申请号：US531721
申请日：1990-06-01
申请人： Richard B. Thompson , Michael Levine
发明人： Richard B. Thompson , Michael Levine
IPC分类号： G01J1/04 , G01J1/42 , G01N21/64
CPC分类号： G01J1/04 , G01J1/0407 , G01J1/0411 , G01J1/0425 , G01J1/044 , G01J1/0455 , G01N21/64 , G01J1/0488 , G01J2001/4242 , G01N2021/6463 , G01N2021/6478 , G01N2021/6484
摘要： A photoluminescence sensor for detecting a photoluminescent light from a toluminescent material is disclosed. In a preferred embodiment the photoluminescence sensor comprises: a source of light; a concave mirror having at least one perforation for passing the source light through the at least one perforation; an optical waveguide having proximal and distal ends with the photoluminescent material being disposed at the distal end; an objective for directing the source light into the proximal end of the waveguide; an objective for receiving photoluminescent light and for focusing the photoluminescent light onto the perforated concave mirror; a liquid filter for passing the photoluminescent light reflected from the perforated concave mirror to a detector to detect the photoluminescent light. The sensor can also include a chopper disposed at the output end of the objective for modulating the light source at a select frequency and a lock-in amplifier tuned to measure the output from the detector at the select frequency.
摘要翻译：公开了一种用于检测来自光致发光材料的光致发光的光致发光传感器。在优选实施例中，光致发光传感器包括：光源; 具有至少一个用于使源光穿过所述至少一个穿孔的穿孔的凹面镜; 具有光致发光材料的近端和远端的光波导被设置在远端; 将源光引导到波导的近端的目的; 用于接收光致发光光并将光致发光光聚焦到穿孔凹面镜上的目的; 液体过滤器，用于将从穿孔凹面镜反射的光致发光传递到检测器以检测光致发光光。传感器还可以包括设置在物镜的输出端的斩波器，用于以选择频率调制光源，并且调节锁定放大器以在选择频率下测量来自检测器的输出。

5. 发明授权

US5083852A Laser beam strop 失效
标题翻译：激光束
公开(公告)号：US5083852A
公开(公告)日：1992-01-28
申请号：US531422
申请日：1990-05-31
申请人： Richard B. Thompson
发明人： Richard B. Thompson
IPC分类号： B23K26/42 , G01J1/58 , G02B5/00 , G02B26/02
CPC分类号： G02B26/02 , B23K26/702 , G01J1/58 , G02B5/005
摘要： A laser beam stop is contructed having a uniquely designed cell filled with solution or suspension of an absorbing species chosen to absorb the wavelength of light emitted by the laser. The position and strength of the laser beam are indicated by the strength and depth of penetration of the fluorescence excited by the laser.
摘要翻译：激光束停止被设计成具有独特设计的单元，填充有吸收物质的溶液或悬浮液，吸收物质被选择以吸收由激光发射的光的波长。激光束的位置和强度由激光激发的荧光的穿透强度和深度表示。

6. 发明授权

US5061857A Waveguide-binding sensor for use with assays 失效
标题翻译：用于测定的波导结合传感器
公开(公告)号：US5061857A
公开(公告)日：1991-10-29
申请号：US610895
申请日：1990-11-09
申请人： Richard B. Thompson , Carl A. Villarruel
发明人： Richard B. Thompson , Carl A. Villarruel
IPC分类号： A61B5/00 , G01N21/64 , G01N21/77
CPC分类号： G01N21/648 , A61B5/1459 , G01N21/6428 , G01N21/7703 , G01N2021/6432 , G01N2021/6434
摘要： An optical waveguide-binding sensor which increases sensor sensitivity to fluorescence detection during assays of liquids based on adjustments in the V number along the optical waveguide. The sensing waveguide includes a mode converting section where the V number of the wavguide gradually increases from the distal sensing end of the mode converting section to the proximal end of the mode converting section. The gradually change in V number can be accomplished by either gradually inwardly tapering waveguide along the sensor portion from the proximal end, or by gradually varying the dopant concentration along the sensing portion. The sensor tapers in diameter to change the V number along the distal end of the optical waveguide. The present invention increases the efficiency of fluorescence detection by converting weakly-guided high-order modes of fluorescence radiation at the distal end into lower-order modes at the proximal and increases the efficiency of fluorescent excitation by converting low order mode entering the proximal end into weakly-guided high order modes at the sensing end. At the sensing end, these weakly guided modes cn strengthen the evanescent excitation wave.
摘要翻译：一种光波导结合传感器，其基于沿着光波导的V数的调节，增加了液体测定期间对荧光检测的灵敏度。感测波导包括模式转换部分，其中W形导体的V数从模式转换部分的远端感测端逐渐增加到模式转换部分的近端。 V数的逐渐变化可以通过从近端沿着传感器部分逐渐向内逐渐变细的波导，或者沿着感测部分逐渐改变掺杂剂浓度来实现。传感器的直径逐渐变细，沿着光波导的远端改变V数。本发明通过将远端的弱引导高阶模式的近端的高阶模式转换为近端的低阶模式来提高荧光检测的效率，并且通过将进入近端的低阶模式转换成低频模式来提高荧光激发的效率弱导向高阶模式在感应端。在感测端，这些弱引导模式cn加强了消逝激发波。

7. 外观设计

USD279911S Rebound ball game net 失效
公开(公告)号：USD279911S
公开(公告)日：1985-07-30
申请号：US474168
申请日：1983-03-10
申请人： Richard B. Thompson
设计人： Richard B. Thompson

8. 发明授权

US2774060A Detecting means for stolen goods 失效
标题翻译：被盗物品的检测手段
公开(公告)号：US2774060A
公开(公告)日：1956-12-11
申请号：US36157553
申请日：1953-06-15
申请人： RICHARD B THOMPSON , DALE BELFORD , OLIVER D OLSON
发明人： THOMPSON THOMAS F
IPC分类号： G01V3/10 , G06K7/08 , G06K19/067 , G08B13/26
CPC分类号： G06K7/086 , G01V3/102 , G06K19/0672 , G08B13/26

9. 发明授权

US09193990B2 Bioluminescent metal ion assay 有权
标题翻译：生物发光金属离子测定
公开(公告)号：US09193990B2
公开(公告)日：2015-11-24
申请号：US14218085
申请日：2014-03-18
申请人： Richard B. Thompson
发明人： Richard B. Thompson , Evgenia Matveeva , Carol Fierke , Leslie Bourne , Graham Franke
IPC分类号： C12Q1/66 , G01N33/542
CPC分类号： C12Q1/66 , G01N33/542 , G01N2333/988
摘要： A method for determining metal ions, both qualitatively and quantitatively, is disclosed. The method utilizes emission from fluorescence resonance energy transfer from a luciferase-carbonic anhydrase conjugate or fusion protein to an acceptor ligand in the presence of metal ion bound to the protein to measure free metal ion concentrations down to picomolar concentration ranges. The method is relatively insensitive to contaminants, and so can be used to measure metal ion concentrations in cells, body fluids or environmental samples without extensive sample preparation.
摘要翻译：本发明公开了一种定性和定量测定金属离子的方法。该方法利用与蛋白质结合的金属离子的存在下从荧光素酶 - 碳酸酐酶偶联物或融合蛋白到受体配体的荧光共振能量转移的发射，以测量低至皮摩尔浓度范围的游离金属离子浓度。该方法对污染物相对不敏感，因此可用于测量细胞，体液或环境样品中的金属离子浓度，而无需广泛的样品制备。

10. 发明授权

US5545517A Selective metal ion detection using a photoluminescent indicator binding to a macromolecule-metal ion complex 失效
标题翻译：使用结合大分子金属离子络合物的光致发光指示剂进行选择性金属离子检测
公开(公告)号：US5545517A
公开(公告)日：1996-08-13
申请号：US213409
申请日：1994-03-15
申请人： Richard B. Thompson , Eric R. Jones
发明人： Richard B. Thompson , Eric R. Jones
IPC分类号： C12Q1/527 , G01N21/64 , G01N31/22 , C12Q1/00 , G01N21/00
CPC分类号： G01N21/643 , C12Q1/527 , G01N31/22 , Y10S435/808 , Y10S436/805
摘要： The present invention is a process and apparatus for metal ion detection. The process of the invention has the steps of (1) disposing, in an analyte medium, a macromolecule suitable for selective complexation with the target metal ion species; (2) disposing, in the analyte medium, an appropriate photoluminescent indicator that will emit in a measurably different manner when bound to the metallomacromolecule complex, compared with its unbound state; (3) exciting the photoluminescent indicator species; and (4) monitoring the emission of the photoluminescent indicator species to detect changes in its emission. The apparatus of the invention has (1) a macromolecule suitable for selective complexation with the target metal ion species disposed in an analyte medium; (2) an appropriate photoluminescent indicator that will emit in a measurably different manner when bound to the metallomacromolecule complex, compared with its unbound state, also disposed in the analyte medium; (3) a source for exciting the photoluminescent indicator species; and (4) a detector for monitoring the emission of the photoluminescent indicator species to detect changes in its emission.
摘要翻译：本发明是用于金属离子检测的方法和装置。本发明的方法具有以下步骤：（1）在分析物介质中设置适于与目标金属离子物质选择性络合的大分子; （2）在分析物介质中，与其未结合状态相比，在与金属大分子复合物结合时以相当不同的方式配置适当的光致发光指示剂; （3）激发光致发光指示物种; 和（4）监测光致发光指示剂物质的发射以检测其发射的变化。本发明的装置具有（1）适用于与分析物介质中配置的靶金属离子种类选择性络合的大分子; （2）当与其未结合状态相比，当与金属大分子复合物结合时，其可以以可测量不同的方式发射，也放置在分析物介质中的合适的光致发光指示剂; （3）激发光致发光指示物种的来源; 和（4）用于监测光致发光指示剂物质的发射以检测其发射变化的检测器。

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