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    • 2. 发明申请
    • Aptamer Coated Measurement and Reference Electrodes and Methods Using Same for Biomarker Detection
    • 适配器涂层测量和参考电极和方法使用相同的生物标志物检测
    • US20140162893A1
    • 2014-06-12
    • US14008622
    • 2012-04-02
    • Stephen Lee CashKeith RobsonIan Anthony KinlochPeter George Stockley
    • Stephen Lee CashKeith RobsonIan Anthony KinlochPeter George Stockley
    • G01N33/543
    • G01N33/5438
    • A device for identifying the presence of a specific target molecule or biomarker by the detection of a change in an electrical property includes a measurement sensor 8 comprising a semiconducting sensor structure 12 capable of conjugating with the biomarker, thus giving rise to the said change in electrical property, and an electrode system 3, 4 for conducting a signal from the device. According to the invention there is a further such sensor 9, of substantially identical form but having its sensor structure 14 already conjugated with the biomarker, or otherwise capped, e.g. using a further oligonucleotide strand, so as to act as an internal reference. When a biological sample, e.g. saliva, is applied to the electrodes, the reference enables the discounting of all environmental effects other than the biomarker. The invention provides a simple, cheap and accurate text for one or more biomarkers that can be used in the field without complex equipment.
    • 用于通过检测电特性的变化来识别特定靶分子或生物标志物的存在的装置包括测量传感器8,其包括能够与生物标志物共轭的半导体传感器结构12,从而导致所述电气变化 以及用于从该装置传导信号的电极系统3,4。 根据本发明,还有一种这样的传感器9,其形状基本相同,但其传感器结构14已经与生物标志物缀合, 使用另外的寡核苷酸链,以作为内部参照。 当生物样品,例如 唾液被施用于电极,该参考物可以折射生物标志物以外的所有环境影响。 本发明为一种或多种生物标志物提供了一种简单,便宜且准确的文本,可用于现场,无需复杂的设备。