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1. 发明申请

US20150155183A1 METHOD AND APPARATUS FOR PULSE ELECTROCHEMICAL POLISHING 有权
标题翻译：脉冲电化学抛光方法与装置
公开(公告)号：US20150155183A1
公开(公告)日：2015-06-04
申请号：US14402853
申请日：2012-05-24
申请人： Jian Wang , Yinuo Jin , Jun Wang , Hui Wang
发明人： Jian Wang , Yinuo Jin , Jun Wang , Hui Wang
IPC分类号： H01L21/321 , C25F3/30 , H01L21/66
CPC分类号： H01L21/32115 , C25F3/16 , C25F3/30 , C25F7/00 , H01L22/12 , H01L22/20
摘要： A method and apparatus for pulse electrochemical polishing a wafer are disclosed. The method comprises steps of: establishing a duty cycle table showing all points on the wafer, a removal thickness corresponding to every point and a duty cycle corresponding to the removal thickness; driving a wafer chuck holding and positioning the wafer to move at a preset speed so that a special point on the wafer is right above a nozzle ejecting charged electrolyte onto the wafer; looking up the duty cycle table and obtaining the removal thickness and the duty cycle corresponding to the special point; and applying a preset pulse power source to the wafer and the nozzle and the actual polishing power source for polishing the special point being equal to the duty cycle multiplying by the preset power source.
摘要翻译：公开了用于脉冲电化学抛光晶片的方法和装置。该方法包括以下步骤：建立表示晶片上所有点的占空比表，对应于每个点的去除厚度和对应于去除厚度的占空比; 驱动晶片卡盘保持并定位晶片以预设速度移动，使得晶片上的特殊点恰好在喷射带电电解质的喷嘴上方到晶片上; 查找占空比表，获得与特殊点对应的去除厚度和占空比; 并且将预设的脉冲电源施加到晶片和喷嘴以及用于抛光特殊点的实际抛光电源等于乘以预设电源的占空比。

2. 发明授权

US09865476B2 Method and apparatus for pulse electrochemical polishing 有权
公开(公告)号：US09865476B2
公开(公告)日：2018-01-09
申请号：US14402853
申请日：2012-05-24
申请人： Jian Wang , Yinuo Jin , Jun Wang , Hui Wang
发明人： Jian Wang , Yinuo Jin , Jun Wang , Hui Wang
IPC分类号： H01L21/321 , C25F3/16 , C25F7/00 , H01L21/66 , C25F3/30
CPC分类号： H01L21/32115 , C25F3/16 , C25F3/30 , C25F7/00 , H01L22/12 , H01L22/20
摘要： A method and apparatus for pulse electrochemical polishing a wafer are disclosed. The method comprises steps of: establishing a duty cycle table showing all points on the wafer, a removal thickness corresponding to every point and a duty cycle corresponding to the removal thickness; driving a wafer chuck holding and positioning the wafer to move at a preset speed so that a special point on the wafer is right above a nozzle ejecting charged electrolyte onto the wafer; looking up the duty cycle table and obtaining the removal thickness and the duty cycle corresponding to the special point; and applying a preset pulse power source to the wafer and the nozzle and the actual polishing power source for polishing the special point being equal to the duty cycle multiplying by the preset power source.

3. 发明申请

US20140323320A1 METHOD OF DETECTING FUSED TRANSCRIPTS AND SYSTEM THEREOF 审中-公开
公开(公告)号：US20140323320A1
公开(公告)日：2014-10-30
申请号：US14369566
申请日：2011-12-31
申请人： Wenlong Jia , Kunlong Qiu , Guangwu Guo , Minghui He , Jun Wang , Jian Wang , Huanming Yang
发明人： Wenlong Jia , Kunlong Qiu , Guangwu Guo , Minghui He , Jun Wang , Jian Wang , Huanming Yang
IPC分类号： G06F19/22
CPC分类号： G16B30/00 , C12Q1/6869 , C12Q2535/122
摘要： Provided is a method of detecting method of detecting fusion transcripts in a sample to be analyzed. The method may comprises: subjecting the sample to be analyzed containing a RNA transcriptome to paired-end sequencing, to obtain paired-end RNA-Seq data of the sample to be analyzed; aligning the paired-end RNA-Seq data to a human reference genome sequence, to obtain first paired-end mapped reads, first single-end mapped reads, and first unmapped reads; evaluating an insertsize between two ends of the paired-end mapped reads by means of the first paired-end mapped reads, to obtain a proportion of paired-end mapped reads with overlapped 3′-ends; aligning the first unmapped reads to annotated transcripts, to obtain second single-end mapped reads and second unmapped reads; aligning the second unmapped reads to the annotated transcripts, to filter out unmapped reads caused by indel and obtain third unmapped reads; merging all single-end mapped reads, to obtain a set of single-end mapped reads; obtaining a gene pair linked by a cross-read as a primary set of candidate gene pairs based on the set of single-end mapped reads and combining with a relationship of the mapped paired-end reads; subjecting the primary set of candidate gene pairs to a filtration, to obtain a candidate set of fused gene pairs; bisecting the third unmapped read, to obtain a half-unmapped read; aligning the half-unmapped read to a gene-junction sequence in the candidate set of fused gene pairs, to obtain a potent region of a fused junction site in the gene in which the half-unmap read locates; outputting original reads of mapped half-unmapped reads, to obtain useful unmapped reads; subjecting the candidate set of fused gene pairs to a fusion simulation; aligning the useful unmapped reads to a junction library, to obtain a fused gene supported by the useful unmapped reads; calculating and gathering the fused sequence supported by the useful unmapped reads, to obtain information of the fused gene. And a system for detecting fusion transcripts is also provided.

4. 发明申请

US20110295784A1 ERROR CORRECTING METHOD OF TEST SEQUENCE, CORRESPONDING SYSTEM AND GENE ASSEMBLY EQUIPMENT 有权
标题翻译：测试顺序错误校正方法，相应系统和基因组装设备
公开(公告)号：US20110295784A1
公开(公告)日：2011-12-01
申请号：US13132038
申请日：2009-12-11
申请人： Jun Wang , Huanming Yang , Jian Wang
发明人： Jun Wang , Huanming Yang , Jian Wang
IPC分类号： G06N3/12
CPC分类号： G06F19/24 , C12Q1/6874 , G06F19/22
摘要： The present invention provides an error correcting method of test sequence, which involves receiving test sequences, configuring high frequency short string list based on a preset high frequency threshold value, traversing each received test sequence, searching an area with the largest number of continuous high frequency short strings on each test sequence in combination with high frequency short string list, configuring whole left sequence and/or right sequence of high frequency short strings at left side and/or right side of searched area according to corresponding received test sequence and high frequency short string list, and constituting corresponding test sequence according to configured left and/or right sequence and searched area. The present invention also provides corresponding error correcting system of test sequence and gene assembly equipment.
摘要翻译：本发明提供一种测试序列的纠错方法，其包括接收测试序列，基于预设的高频阈值配置高频短串列表，遍历每个接收的测试序列，搜索具有最大数目的连续高频区域每个测试序列上的短串组合高频短串列表，根据相应的接收测试序列和高频短信配置搜索区域的左侧和/或右侧的全部左序列和/或右序列的高频短串字符串列表，并根据配置的左和/或右序列和搜索区域构成对应的测试序列。本发明还提供了相应的测试序列和基因组装设备的纠错系统。

5. 发明授权

US09857377B2 Method for quantification of proteome 有权
公开(公告)号：US09857377B2
公开(公告)日：2018-01-02
申请号：US14369825
申请日：2011-12-31
申请人： Haiyi Zhao , Dahai Jiang , Bo Wen , Jian Wang , Jun Wang , Huanming Yang , Jin Zi , Yan Ren , Siqi Liu
发明人： Haiyi Zhao , Dahai Jiang , Bo Wen , Jian Wang , Jun Wang , Huanming Yang , Jin Zi , Yan Ren , Siqi Liu
IPC分类号： G01N33/68 , G06F19/18 , H01J49/00
CPC分类号： G01N33/6848 , G01N33/68 , G01N2570/00 , G06F19/18 , H01J49/0036
摘要： The present invention relates to a method for quantifying the relative content of a protein in a sample. The present invention also relates to a method for comprising the relative content of a protein in at least two samples.

6. 发明申请

US20140249038A1 Method of detecting a pre-determined event in a nucleic acid sample and system thereof 审中-公开
标题翻译：检测核酸样品中的预定事件的方法及其系统
公开(公告)号：US20140249038A1
公开(公告)日：2014-09-04
申请号：US14351468
申请日：2011-12-21
申请人： Hui Jiang , Fang Chen , Huijuan Ge , Peipei Li , Xuchao Li , Jian Wang , Jun Wang , Huanming Yang , Xiuqing Zhang
发明人： Hui Jiang , Fang Chen , Huijuan Ge , Peipei Li , Xuchao Li , Jian Wang , Jun Wang , Huanming Yang , Xiuqing Zhang
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6869 , C12Q1/6883 , C12Q2600/156
摘要： Disclosed are a method of detecting a pre-determined event in a nucleic acid sample and a system thereof. The method of detecting the pre-determined event in the nucleic acid sample comprises the following steps: constructing a sequencing-library for the nucleic acid sample; sequencing the sequencing-library to obtain a sequencing result consisting of a plurality of sequencing data; determining the sequencing data from a pre-determined region; and determining an occurrence of the pre-determined event in the nucleic acid sample based on a composition of the sequencing data from the pre-determined region.
摘要翻译：公开了一种在核酸样品及其系统中检测预定事件的方法。检测核酸样品中预先确定事件的方法包括以下步骤：构建核酸样品的测序文库; 测序序列库以获得由多个测序数据组成的测序结果; 确定来自预定区域的测序数据; 以及基于来自预定区域的测序数据的组成确定核酸样品中预定事件的发生。

7. 发明授权

US08751165B2 Error correcting method of test sequence, corresponding system and gene assembly equipment 有权
标题翻译：测试序列错误纠正方法，相应的系统和基因组装设备
公开(公告)号：US08751165B2
公开(公告)日：2014-06-10
申请号：US13132038
申请日：2009-12-11
申请人： Jun Wang , Huanming Yang , Jian Wang
发明人： Jun Wang , Huanming Yang , Jian Wang
IPC分类号： G01N33/48 , G06F19/00 , G06F19/24
CPC分类号： G06F19/24 , C12Q1/6874 , G06F19/22
摘要： The present invention provides an error correcting method of test sequence, which involves receiving test sequences, configuring high frequency short string list based on a preset high frequency threshold value, traversing each received test sequence, searching an area with the largest number of continuous high frequency short strings on each test sequence in combination with high frequency short string list, configuring whole left sequence and/or right sequence of high frequency short strings at left side and/or right side of searched area according to corresponding received test sequence and high frequency short string list, and constituting corresponding test sequence according to configured left and/or right sequence and searched area. The present invention also provides corresponding error correcting system of test sequence and gene assembly equipment.
摘要翻译：本发明提供一种测试序列的纠错方法，其包括接收测试序列，基于预设的高频阈值配置高频短串列表，遍历每个接收的测试序列，搜索具有最大数目的连续高频区域每个测试序列上的短串组合高频短串列表，根据相应的接收测试序列和高频短信配置搜索区域的左侧和/或右侧的全部左序列和/或右序列的高频短串字符串列表，并根据配置的左和/或右序列和搜索区域构成对应的测试序列。本发明还提供了相应的测试序列和基因组装设备的纠错系统。

8. 发明申请

US20150242565A1 METHOD AND DEVICE FOR ANALYZING MICROBIAL COMMUNITY COMPOSITION 审中-公开
标题翻译：用于分析微生物群落组成的方法和装置
公开(公告)号：US20150242565A1
公开(公告)日：2015-08-27
申请号：US14419060
申请日：2012-08-01
申请人： Shenghui Li , Qiang Feng , Junjie Qin , Jianfeng Zhu , Yuanlin Guan , Jun Wang , Jian Wang , Huanming Yang
发明人： Shenghui Li , Qiang Feng , Junjie Qin , Jianfeng Zhu , Yuanlin Guan , Jun Wang , Jian Wang , Huanming Yang
IPC分类号： G06F19/16
CPC分类号： G16B15/00 , C12Q1/6809 , C12Q1/689 , G16B20/00 , G16B30/00 , C12Q2535/122 , C12Q2537/165
摘要： The present teachings relate to analysis of a sample including a plurality of species. In one example, sequences of fragments of polynucleotides of the sample are obtained. A reference set comprising a plurality of sequences is obtained. An initial bin for each of the plurality of sequences of the reference set is determined based on a relative abundance of each sequence of the reference set in the sample. At least one final bin is obtained by modifying the initial bins for the plurality of sequences based on a model.
摘要翻译：本教导涉及包括多个物种的样品的分析。在一个实例中，获得样品的多核苷酸的片段序列。获得包括多个序列的参考集。基于样本中的参考集合的每个序列的相对丰度来确定参考集合的多个序列中的每一个的初始bin。通过基于模型修改多个序列的初始仓，获得至少一个最终仓。

9. 发明申请

US20150141290A1 METHOD FOR QUANTIFICATION OF PROTEOME 有权
标题翻译：保护方法
公开(公告)号：US20150141290A1
公开(公告)日：2015-05-21
申请号：US14369825
申请日：2011-12-31
申请人： Haiyi Zhao , Dahai Jiang , Bo Wen , Jian Wang , Jun Wang , Huanming Yang
发明人： Haiyi Zhao , Dahai Jiang , Bo Wen , Jian Wang , Jun Wang , Huanming Yang
IPC分类号： G01N33/68 , H01J49/00
CPC分类号： G01N33/6848 , G01N33/68 , G01N2570/00 , G06F19/18 , H01J49/0036
摘要： The present invention relates to a method for quantifying the relative content of a protein in a sample. The present invention also relates to a method for comprising the relative content of a protein in at least two samples.
摘要翻译：本发明涉及量化样品中蛋白质相对含量的方法。本发明还涉及在至少两个样品中包含蛋白质的相对含量的方法。

10. 发明申请

US20150120256A1 METHOD OF RECONSTRUCTING HAPLOTYPE OF DIPLOID AND SYSTEM THEREOF 审中-公开
标题翻译：重复二倍体皮脂的方法及其系统
公开(公告)号：US20150120256A1
公开(公告)日：2015-04-30
申请号：US14369604
申请日：2012-05-31
申请人： Shujia Huang , Peng Sun , Honglong Wu , Jian Wang , Jun Wang , Huanming Yang
发明人： Shujia Huang , Peng Sun , Honglong Wu , Jian Wang , Jun Wang , Huanming Yang
IPC分类号： G06F19/12 , G06F17/16 , G06F17/50
CPC分类号： G16B5/00 , G06F17/16 , G06F17/5009 , G16B20/00 , G16B40/00
摘要： Provided is a method and system of reconstructing a haplotype of a diploid. The method can include constructing a matrix of sequence fragments consisting of ternary character based on sequence fragments comprising at least one common site, wherein in the matrix of sequence fragments, two allelic bases of an SNP site in chromosome fragments are labeled with A and B respectively; initializing two fragment sets of based on the matrix of sequence fragments; determining an objective function and an initial reference temperature; performing a process of simulated annealing based on the objective function and the initial reference temperature, and outputting final sets until a convergence criteria is achieved; inferring a haplotype based on the final sets by means of minimum error correction.
摘要翻译：提供了重建二倍体的单体型的方法和系统。该方法可以包括构建基于包含至少一个共同位点的序列片段的由三元字符组成的序列片段的矩阵，其中在序列片段的基序中，染色体片段中的SNP位点的两个等位基因分别用A和B标记 ; 基于序列片段的矩阵初始化两个片段集合; 确定目标函数和初始参考温度; 基于目标函数和初始参考温度执行模拟退火过程，并输出最终集合直到达到收敛标准; 通过最小误差校正推导出基于最终集合的单倍型。

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