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1. 发明申请

US20180292320A1 METHODS AND SYSTEMS FOR BIOLOGICAL INSTRUMENT CALIBRATION 审中-公开
公开(公告)号：US20180292320A1
公开(公告)日：2018-10-11
申请号：US16010359
申请日：2018-06-15
申请人： Life Technologies Corporation
发明人： Yong Chu , Jeffrey Marks , Jacob Freudenthal , Thomas Wessel , David Woo
IPC分类号： G01N21/64 , C12Q1/686 , G01N21/27 , C12Q1/6851
摘要： In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging fluorescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration to determine reaction site positions in an image. The method further includes performing a pure dye calibration to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration to determine a filter normalization factor. The method includes performing an RNase P validation to validate the instrument is capable of distinguishing between two different quantities of sample.

2. 发明申请

US20140294268A1 Methods and Systems for Streamlining Optical Calibration 有权
标题翻译：简化光学校准的方法和系统
公开(公告)号：US20140294268A1
公开(公告)日：2014-10-02
申请号：US14348206
申请日：2012-09-30
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Jacob K. Freudenthal , Jeffrey A. Marks , Francis T. Cheng , Thomas Wessel
IPC分类号： G06T7/00
CPC分类号： G06T7/0012 , G06T5/008 , G06T5/50 , G06T2207/10024 , G06T2207/10064 , G06T2207/10144 , G06T2207/20021 , G06T2207/20216 , G06T2207/30072
摘要： A method for calibrating a biological instrument is provided. The method comprises the steps of acquiring an image of at least one biological sample array, determining a first region of interest within the image, wherein the first region of interest comprises a first plurality of locations on the at least one biological array; and identifying within the first region of interest, a plurality of image elements associated with each of the first plurality of locations on the at least one biological array.
摘要翻译：提供了一种用于校准生物仪器的方法。该方法包括以下步骤：获取至少一个生物样本阵列的图像，确定图像内的第一感兴趣区域，其中所述第一感兴趣区域包括所述至少一个生物阵列上的第一多个位置; 以及在所述第一感兴趣区域内识别与所述至少一个生物阵列上的所述第一多个位置中的每一个相关联的多个图像元素。

3. 发明授权

US10147182B2 Methods and systems for streamlining optical calibration 有权
公开(公告)号：US10147182B2
公开(公告)日：2018-12-04
申请号：US15350444
申请日：2016-11-14
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Jacob Freudenthal , Jeffrey Marks , Francis Cheng , Thomas Wessel
IPC分类号： G06K9/00 , G06T7/00 , G06T5/00 , G06T5/50
摘要： A method for calibrating a biological instrument is provided. The method comprises the steps of acquiring an image of at least one biological sample array, determining a first region of interest within the image, wherein the first region of interest comprises a first plurality of locations on the at least one biological array; and identifying within the first region of interest, a plurality of image elements associated with each of the first plurality of locations on the at least one biological array.

4. 发明申请

US20160231245A1 METHODS AND SYSTEMS FOR INSTRUMENT VALIDATION 审中-公开
标题翻译：仪器验证的方法和系统
公开(公告)号：US20160231245A1
公开(公告)日：2016-08-11
申请号：US15016485
申请日：2016-02-05
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Thomas Wessel , Yong Chu , Jacob Freudenthal , David Woo
IPC分类号： G01N21/64
CPC分类号： G01N21/6428 , G01N21/274 , G01N21/6452 , G01N21/6456 , G01N2021/6439 , G16B40/00
摘要： In one exemplary embodiment, a method for validating an instrument is provided. The method includes receiving amplification data from a validation plate to generate a plurality of amplification curves. The validation plate includes a sample of a first quantity and a second quantity, and each amplification curve includes an exponential region. The method further includes determining a set of fluorescence thresholds based on the exponential regions of the plurality of amplification curves and determining, for each fluorescence threshold of the set, a first set of cycle threshold (Ct) values of amplification curves generated from the samples of the first quantity and a second set of Ct values of amplification curves generated from the samples of the second quantity. The method includes calculating if the first and second quantities are sufficiently distinguishable based on Ct values at each of the plurality of fluorescence thresholds.
摘要翻译：在一个示例性实施例中，提供了用于验证仪器的方法。该方法包括从验证板接收放大数据以产生多个扩增曲线。验证板包括第一数量和第二数量的样本，并且每个扩增曲线包括指数区域。该方法还包括基于多个扩增曲线的指数区域来确定一组荧光阈值，并且针对该组的每个荧光阈值确定从样品中产生的扩增曲线的第一组循环阈值（Ct）值从第二数量的样本产生的第一数量和第二组扩增曲线的Ct值。该方法包括基于多个荧光阈值中的每个荧光阈值处的Ct值来计算第一和第二量是否足够可区分。

5. 发明申请

US20210313010A1 METHODS AND SYSTEMS FOR A DIGITAL PCR EXPERIMENT DESIGNER 有权
公开(公告)号：US20210313010A1
公开(公告)日：2021-10-07
申请号：US17207488
申请日：2021-03-19
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Nivedita Sumi Majumdar , Thomas Wessel , David C. Woo , Marcin Sikora , Gordon A. Janaway , Shweta Raizada , Joanna Lankester , Jeffrey A. Marks , Daniel Wessel
IPC分类号： G16B25/20 , G16B25/00 , G16B40/00 , C12Q1/686
摘要： A computer-implemented method for designing a digital PCR (dPCR) experiment is provided. The method includes receiving, from a user, a selection of optimization type. The optimization type may be maximizing the dynamic range, minimizing the number of substrates including reaction sites needed for the experiment, determining a dilution factor, or determining the lower limit of detection, for example. The method further includes receiving, from the user, a precision measure for an experiment, and a minimum concentration of a target in a reaction site for the experiment. The method also includes determining a set of dPCR experiment design factors for the experiment based on the optimization type. The set of dPCR experiment design factors is then displayed to the user.

6. 发明授权

US11004540B2 Determining the limit of detection of rare targets using digital PCR 有权
公开(公告)号：US11004540B2
公开(公告)日：2021-05-11
申请号：US15579407
申请日：2016-06-03
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Nivedita Sumi Majumdar , Thomas Wessel
IPC分类号： G16B40/00 , G06N7/00
摘要： A method for determining false positives calls in a biological data plot is provided. The method includes identifying a first data cluster as non-amplification data points within the biological data plot and identifying a second data cluster as wild-type positives within the biological data plot. The method further includes estimating a position in the biological data plot of a center of the first and second data clusters. The method further includes determining, for each data point within the first data cluster, a probability of belonging to the first data cluster and determining, for each data point within the second data cluster, a probability of belonging to the second data cluster. The method includes applying a probability threshold for each data point within the first and second data cluster to identify false positives.

7. 发明授权

US10012590B2 Methods and systems for biological instrument calibration 有权
公开(公告)号：US10012590B2
公开(公告)日：2018-07-03
申请号：US15017249
申请日：2016-02-05
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Yong Chu , Jeffrey Marks , Jacob Freudenthal , Thomas Wessel , David Woo
IPC分类号： H01J40/14 , G01N21/64 , C12Q1/686 , G01N21/27 , C12Q1/6851
CPC分类号： G01N21/6428 , C12Q1/6851 , C12Q1/686 , G01N21/274 , G01N21/278 , G01N21/6452 , G01N21/6456 , G01N2021/6439 , G01N2021/6471 , G01N2201/127 , G01N2201/13 , G01N2333/922
摘要： In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging florescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration to determine reaction site positions in an image. The method further includes performing a pure dye calibration to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration to determine a filter normalization factor. The method includes performing an RNase P validation to validate the instrument is capable of distinguishing between two different quantities of sample.

8. 发明授权

US09495741B2 Methods and systems for streamlining optical calibration 有权
标题翻译：简化光学校准的方法和系统
公开(公告)号：US09495741B2
公开(公告)日：2016-11-15
申请号：US14348206
申请日：2012-09-30
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Jacob K. Freudenthal , Jeffrey A. Marks , Francis T. Cheng , Thomas Wessel
IPC分类号： G06K9/00 , G06T7/00 , G06T5/00 , G06T5/50
CPC分类号： G06T7/0012 , G06T5/008 , G06T5/50 , G06T2207/10024 , G06T2207/10064 , G06T2207/10144 , G06T2207/20021 , G06T2207/20216 , G06T2207/30072
摘要： A method for calibrating a biological instrument is provided. The method comprises the steps of acquiring an image of at least one biological sample array, determining a first region of interest within the image, wherein the first region of interest comprises a first plurality of locations on the at least one biological array; and identifying within the first region of interest, a plurality of image elements associated with each of the first plurality of locations on the at least one biological array.
摘要翻译：提供了一种用于校准生物仪器的方法。该方法包括以下步骤：获取至少一个生物样本阵列的图像，确定图像内的第一感兴趣区域，其中所述第一感兴趣区域包括所述至少一个生物阵列上的第一多个位置; 以及在所述第一感兴趣区域内识别与所述至少一个生物阵列上的所述第一多个位置中的每一个相关联的多个图像元素。

9. 发明申请

US20160231246A1 METHODS AND SYSTEMS FOR BIOLOGICAL INSTRUMENT CALIBRATION 有权
标题翻译：生物仪器校准的方法和系统
公开(公告)号：US20160231246A1
公开(公告)日：2016-08-11
申请号：US15017249
申请日：2016-02-05
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Yong Chu , Jeffrey Marks , Jacob Freudenthal , Thomas Wessel , David Woo
IPC分类号： G01N21/64 , C12Q1/68
CPC分类号： G01N21/6428 , C12Q1/6851 , C12Q1/686 , G01N21/274 , G01N21/278 , G01N21/6452 , G01N21/6456 , G01N2021/6439 , G01N2021/6471 , G01N2201/127 , G01N2201/13 , G01N2333/922
摘要： In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging florescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration to determine reaction site positions in an image. The method further includes performing a pure dye calibration to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration to determine a filter normalization factor. The method includes performing an RNase P validation to validate the instrument is capable of distinguishing between two different quantities of sample.
摘要翻译：在一个示例性实施例中，提供了一种用于校准仪器的方法。该仪器包括能够成像来自多个反应位点的荧光发射的光学系统。该方法包括执行感兴趣区域（ROI）校准以确定图像中的反应位置位置。该方法还包括进行纯染料校准，以通过将荧光染料的原始光谱与荧光染料的纯光谱校准数据进行比较来确定每个反应位点中使用的荧光染料的贡献。该方法还包括执行仪器归一化校准以确定滤波器归一化因子。该方法包括执行RNase P验证以验证仪器能够区分两种不同数量的样品。

10. 发明申请

US20150142323A1 METHOD AND SYSTEM FOR DETERMINING AN AMPLIFICATION QUALITY METRIC 审中-公开
标题翻译：用于确定放大质量公差的方法和系统
公开(公告)号：US20150142323A1
公开(公告)日：2015-05-21
申请号：US13261898
申请日：2012-09-28
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Thomas Wessel , Jamie Cho , Howard Weiss , Eric Van Helene , Thomas B. Morrison
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , G16B40/00 , G16B45/00
摘要： According to one exemplary embodiment, a method for providing a amplification quality metric to a user is provided. The method includes receiving amplification data from an amplification of a sample to generate an amplification curve. The amplification curve includes an exponential region and a transition region. The method further includes determining a first value of the transition region and determining a second value of the transition region. The first value is the beginning of the transition region and the second value is the end of the transition region. Next, the amplification quality metric is calculated based on at least the first value and the second value. Then, the amplification quality metric is displayed to the user.
摘要翻译：根据一个示例性实施例，提供了一种用于向用户提供放大质量度量的方法。该方法包括从样品的扩增接收扩增数据以产生扩增曲线。扩增曲线包括指数区域和过渡区域。该方法还包括确定过渡区域的第一值并确定过渡区域的第二值。第一个值是过渡区域的开始，第二个值是过渡区域的结束。接下来，基于至少第一值和第二值来计算放大质量度量。然后，向用户显示放大质量度量。

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