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1. 发明授权

US08575425B2 AXMI-205 pesticidal gene and methods for its use 有权
标题翻译： AXMI-205杀虫基因及其使用方法
公开(公告)号：US08575425B2
公开(公告)日：2013-11-05
申请号：US12828594
申请日：2010-07-01
申请人： Nalini Manoj Desai , Jill Hinson , Deepa Balasubramanian , Kimberly S. Sampson , Daniel J. Tomso , Duane Alan Lehtinen , Nicholas Duck , Rong Guo , Volker Heinrichs
发明人： Nalini Manoj Desai , Jill Hinson , Deepa Balasubramanian , Kimberly S. Sampson , Daniel J. Tomso , Duane Alan Lehtinen , Nicholas Duck , Rong Guo , Volker Heinrichs
IPC分类号： A01H5/00 , C07K14/22 , A01N37/18 , C12N15/31 , C12N15/63 , C12N15/82 , C12N1/21
CPC分类号： C07K14/195 , C07K14/22 , C07K16/1203 , C12N15/8285 , C12N15/8286 , C12Q1/6876 , Y02A40/162 , Y02A40/164
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 3, or 4, the nucleotide sequence set forth in SEQ ID NO:1, 9, 10, or 11, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2,3或4所示氨基酸序列的核苷酸序列的核酸分子，SEQ ID NO：1,9,10或11所示的核苷酸序列，以及其变体和片段。

2. 发明申请

US20110023184A1 AXMI-205 PESTICIDAL GENE AND METHODS FOR ITS USE 有权
标题翻译： AXMI-205农药基因及其使用方法
公开(公告)号：US20110023184A1
公开(公告)日：2011-01-27
申请号：US12828594
申请日：2010-07-01
申请人： Nalini Manoj Desai , Jill Hinson , Deepa Balasubramanian , Kimberly S. Sampson , Daniel J. Tomso , Duane Alan Lehtinen , Nicholas Duck , Rong Guo
发明人： Nalini Manoj Desai , Jill Hinson , Deepa Balasubramanian , Kimberly S. Sampson , Daniel J. Tomso , Duane Alan Lehtinen , Nicholas Duck , Rong Guo
IPC分类号： C12N15/82 , C07H21/00 , C12N5/10 , C12N1/21 , A01H5/00 , C07K14/195 , C07K16/12 , A01N63/02 , A01P7/04 , C12P21/02 , A01H5/10
CPC分类号： C07K14/195 , C07K14/22 , C07K16/1203 , C12N15/8285 , C12N15/8286 , C12Q1/6876 , Y02A40/162 , Y02A40/164
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 3, or 4, the nucleotide sequence set forth in SEQ ID NO:1, 9, 10, or 11, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2,3或4所示氨基酸序列的核苷酸序列的核酸分子，SEQ ID NO：1,9,10或11所示的核苷酸序列，以及其变体和片段。

3. 发明授权

US08334431B2 AXMI-115, AXMI-113, AXMI-005, AXMI-163 and AXMI-184: insecticidal proteins and methods for their use 有权
标题翻译： AXMI-115，AXMI-113，AXMI-005，AXMI-163和AXMI-184：其杀虫蛋白及其使用方法
公开(公告)号：US08334431B2
公开(公告)日：2012-12-18
申请号：US12497221
申请日：2009-07-02
申请人： Kimberly S. Sampson , Daniel J. Tomso , Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Shruti Agarwal , Brian McNulty , Chris Campbell , Volker Heinrichs
发明人： Kimberly S. Sampson , Daniel J. Tomso , Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Shruti Agarwal , Brian McNulty , Chris Campbell , Volker Heinrichs
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/32 , C07K14/325 , A01N37/18
CPC分类号： C12N15/8286 , A01N37/18 , C07K14/32 , C07K14/325 , C07K16/1278 , Y02A40/162
摘要： Compositions and methods for conferring insecticidal activity to host cells are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in host cells. Compositions also comprise transformed host cells. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 6, 13, or 14, or the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 11, or 12, as well as variants and fragments thereof.
摘要翻译：提供了赋予宿主细胞杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在宿主细胞中转化和表达。组合物还包含转化的宿主细胞。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：4,5,6,13或14所示氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列， 2,3,11或12，以及其变体和片段。

4. 发明授权

US08791326B2 AXMI-150 delta-endotoxin gene and methods for its use 有权
标题翻译： AXMI-150δ-内毒素基因及其使用方法
公开(公告)号：US08791326B2
公开(公告)日：2014-07-29
申请号：US13302428
申请日：2011-11-22
申请人： Kimberly S. Sampson , Daniel J. Tomso , Volker Heinrichs
发明人： Kimberly S. Sampson , Daniel J. Tomso , Volker Heinrichs
IPC分类号： C12N15/63 , C12N15/82 , C12N1/21 , C12N5/10 , A01H5/00
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, the nucleotide sequence set forth in SEQ ID NO:1, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包括杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。具体而言，本发明提供了具有编码SEQ ID NO：2所示氨基酸序列的核苷酸序列的分离的核酸分子，即SEQ ID NO：1,3,4,5,6,7， 8，9，10，11或12，以及其变体和片段。

5. 发明申请

US20120073016A1 AXMI-150 DELTA-ENDOTOXIN GENE AND METHODS FOR ITS USE 有权
标题翻译： AXMI-150 DELTA-ENDOTOXIN基因及其使用方法
公开(公告)号：US20120073016A1
公开(公告)日：2012-03-22
申请号：US13302428
申请日：2011-11-22
申请人： Kimberly S. Sampson , Daniel J. Tomso , Volker Heinrichs
发明人： Kimberly S. Sampson , Daniel J. Tomso , Volker Heinrichs
IPC分类号： A01H1/06 , C12N15/82 , A01H5/00 , C12N1/21 , C12N5/10 , C12N15/32 , C12N15/63
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, the nucleotide sequence set forth in SEQ ID NO:1, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包括杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了具有编码SEQ ID NO：2所示氨基酸序列的核苷酸序列的分离的核酸分子，SEQ ID NO：1，3，4，5，6，7， 8，9，10，11或12，以及其变体和片段。

6. 发明申请

US20100004176A1 AXMI-115, AXMI-113, AXMI-005, AXMI-163 AND AXMI-184: INSECTICIDAL PROTEINS AND METHODS FOR THEIR USE 有权
标题翻译： AXMI-115，AXMI-113，AXMI-005，AXMI-163和AXMI-184：杀虫蛋白及其使用方法
公开(公告)号：US20100004176A1
公开(公告)日：2010-01-07
申请号：US12497221
申请日：2009-07-02
申请人： Kimberly S. Sampson , Daniel J. Tomso , Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Shruti Agarwal , Brian McNulty , Chris Campbell , Volker Heinrichs
发明人： Kimberly S. Sampson , Daniel J. Tomso , Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Shruti Agarwal , Brian McNulty , Chris Campbell , Volker Heinrichs
IPC分类号： A61K38/16 , C07H21/04 , C12N15/63 , C12N5/00 , C07K14/00 , C07K16/00
CPC分类号： C12N15/8286 , A01N37/18 , C07K14/32 , C07K14/325 , C07K16/1278 , Y02A40/162
摘要： Compositions and methods for conferring insecticidal activity to host cells are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in host cells. Compositions also comprise transformed host cells. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 6, 13, or 14, or the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 11, or 12, as well as variants and fragments thereof.
摘要翻译：提供了赋予宿主细胞杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在宿主细胞中转化和表达。组合物还包含转化的宿主细胞。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：4,5,6,13或14所示氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列， 2,3,11或12，以及其变体和片段。

7. 发明申请

US20090144852A1 AXMI-066 AND AXMI-076: DELTA-ENDOTOXIN PROTEINS AND METHODS FOR THEIR USE 审中-公开
标题翻译： AXMI-066和AXMI-076：DELTA-内毒素蛋白及其使用方法
公开(公告)号：US20090144852A1
公开(公告)日：2009-06-04
申请号：US12252453
申请日：2008-10-16
申请人： Daniel J. Tomso , Nalini Desai , Shruti Agarwal , Kimberly S. Sampson , Sandra Volrath , Volker Heinrichs
发明人： Daniel J. Tomso , Nalini Desai , Shruti Agarwal , Kimberly S. Sampson , Sandra Volrath , Volker Heinrichs
IPC分类号： C12N15/82 , A01H5/00 , C12N15/11 , C12N15/00 , C12N1/21 , C12N5/04 , C07K14/00 , A61K38/16 , C12P21/02
CPC分类号： C07K14/325 , C12N15/8285 , C12N15/8286 , Y02A40/162 , Y02A40/164
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:5, 2, or 10, the nucleotide sequence set forth in SEQ ID NO:4, 1, 3, 4, 6, 9, or 11, or the nucleotide sequence deposited in a bacterial host as Accession No. B-50045, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：5,2或10所示氨基酸序列的核苷酸序列，SEQ ID NO：4,1,3,4 ，6,9或11个核苷酸序列，或以细菌宿主保藏号B-50045以及其变体和片段沉积的核苷酸序列。

8. 发明授权

US08084416B2 AXMI-150 delta-endotoxin gene and methods for its use 有权
标题翻译： AXMI-150δ-内毒素基因及其使用方法
公开(公告)号：US08084416B2
公开(公告)日：2011-12-27
申请号：US12646004
申请日：2009-12-23
申请人： Kimberly S. Sampson , Daniel J. Tomso , Volker Heinrichs
发明人： Kimberly S. Sampson , Daniel J. Tomso , Volker Heinrichs
IPC分类号： C07K14/00 , A01N33/00 , A01P15/00
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, the nucleotide sequence set forth in SEQ ID NO:1, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包括杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。具体而言，本发明提供了具有编码SEQ ID NO：2所示氨基酸序列的核苷酸序列的分离的核酸分子，即SEQ ID NO：1,3,4,5,6,7， 8，9，10，11或12，以及其变体和片段。

9. 发明申请

US20100162439A1 PESTICIDAL GENES FROM BREVIBACILLUS AND METHODS FOR THEIR USE 审中-公开
标题翻译：来自BREVIBACILLUS的杀虫剂基因及其使用方法
公开(公告)号：US20100162439A1
公开(公告)日：2010-06-24
申请号：US12644632
申请日：2009-12-22
申请人： Kimberly S. Sampson , Daniel J. Tomso , Rong Guo
发明人： Kimberly S. Sampson , Daniel J. Tomso , Rong Guo
IPC分类号： A01N37/44 , C07K14/195 , C07H21/04 , C12N15/63 , C12N1/21 , C12N5/04 , A01H5/00 , A01H5/10 , C07K16/12 , C12P21/02 , A01H1/00
CPC分类号： C12N15/8286 , C07K14/195 , C07K14/325 , C07K14/345 , Y02A40/162
摘要： Compositions and methods for conferring insecticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for a Brevibacillus-derived delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 7, or 10, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 6, 8, 9, 11, 12, 13, 14, or 15, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含短杆菌属来源的δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。特别地，本发明提供了具有编码SEQ ID NO：2,4,7或10所示的氨基酸序列的核苷酸序列的分离的核酸分子或者SEQ ID NO： 5，6，8，9，11，12，13，14或15，以及其变体和片段。

10. 发明申请

US20140223599A1 AXMI279 PESTICIDAL GENE AND METHODS FOR ITS USE 审中-公开
标题翻译： AXMI279农药基因及其使用方法
公开(公告)号：US20140223599A1
公开(公告)日：2014-08-07
申请号：US14233435
申请日：2012-07-27
申请人： Kimberly S. Sampson , Deepa Balasubramanian , Duane A. Lehtinen
发明人： Kimberly S. Sampson , Deepa Balasubramanian , Duane A. Lehtinen
IPC分类号： C12N15/82 , A01H5/10 , A01H5/12 , C07K14/195
CPC分类号： C12N15/8286 , A01H5/10 , A01H5/12 , C07K14/195 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 3, or 4, the nucleotide sequence set forth in SEQ ID NO: 1, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2,3或4所示氨基酸序列，SEQ ID NO：1所示核苷酸序列以及变体和片段的核苷酸序列的核酸分子其中。

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