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1. 发明授权

US08575425B2 AXMI-205 pesticidal gene and methods for its use 有权
标题翻译： AXMI-205杀虫基因及其使用方法
公开(公告)号：US08575425B2
公开(公告)日：2013-11-05
申请号：US12828594
申请日：2010-07-01
申请人： Nalini Manoj Desai , Jill Hinson , Deepa Balasubramanian , Kimberly S. Sampson , Daniel J. Tomso , Duane Alan Lehtinen , Nicholas Duck , Rong Guo , Volker Heinrichs
发明人： Nalini Manoj Desai , Jill Hinson , Deepa Balasubramanian , Kimberly S. Sampson , Daniel J. Tomso , Duane Alan Lehtinen , Nicholas Duck , Rong Guo , Volker Heinrichs
IPC分类号： A01H5/00 , C07K14/22 , A01N37/18 , C12N15/31 , C12N15/63 , C12N15/82 , C12N1/21
CPC分类号： C07K14/195 , C07K14/22 , C07K16/1203 , C12N15/8285 , C12N15/8286 , C12Q1/6876 , Y02A40/162 , Y02A40/164
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 3, or 4, the nucleotide sequence set forth in SEQ ID NO:1, 9, 10, or 11, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2,3或4所示氨基酸序列的核苷酸序列的核酸分子，SEQ ID NO：1,9,10或11所示的核苷酸序列，以及其变体和片段。

2. 发明申请

US20110023184A1 AXMI-205 PESTICIDAL GENE AND METHODS FOR ITS USE 有权
标题翻译： AXMI-205农药基因及其使用方法
公开(公告)号：US20110023184A1
公开(公告)日：2011-01-27
申请号：US12828594
申请日：2010-07-01
申请人： Nalini Manoj Desai , Jill Hinson , Deepa Balasubramanian , Kimberly S. Sampson , Daniel J. Tomso , Duane Alan Lehtinen , Nicholas Duck , Rong Guo
发明人： Nalini Manoj Desai , Jill Hinson , Deepa Balasubramanian , Kimberly S. Sampson , Daniel J. Tomso , Duane Alan Lehtinen , Nicholas Duck , Rong Guo
IPC分类号： C12N15/82 , C07H21/00 , C12N5/10 , C12N1/21 , A01H5/00 , C07K14/195 , C07K16/12 , A01N63/02 , A01P7/04 , C12P21/02 , A01H5/10
CPC分类号： C07K14/195 , C07K14/22 , C07K16/1203 , C12N15/8285 , C12N15/8286 , C12Q1/6876 , Y02A40/162 , Y02A40/164
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 3, or 4, the nucleotide sequence set forth in SEQ ID NO:1, 9, 10, or 11, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2,3或4所示氨基酸序列的核苷酸序列的核酸分子，SEQ ID NO：1,9,10或11所示的核苷酸序列，以及其变体和片段。

3. 发明申请

US20120144520A1 Plants with Improved Nitrogen Utilization and Stress Tolerance 审中-公开
标题翻译：具有改善氮利用和胁迫耐受性的植物
公开(公告)号：US20120144520A1
公开(公告)日：2012-06-07
申请号：US13309308
申请日：2011-12-01
申请人： James McLaren , Nicholas Duck , Brian Vande Berg , Alissa Schawalder , Vadim Beilinson , Jill Hinson
发明人： James McLaren , Nicholas Duck , Brian Vande Berg , Alissa Schawalder , Vadim Beilinson , Jill Hinson
IPC分类号： A01H5/00 , C12N5/10 , C12N1/21 , C12N15/82 , A01H5/10
CPC分类号： C12N15/8271 , C12N15/8216 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, or both and that have been transformed using a novel vector construct including nucleic acid sequences that modulate nitrogen use in plants. In various embodiments, the vector construct comprises a nucleic acid sequence selected from the group consisting of SEQ ID NO: 2, 4, 7, 9, 11, 13, 15, 17, 22, 24, 26, 28, 30, 32, 34, 36, or 38. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.
摘要翻译：本发明涉及具有增加的氮利用效率，胁迫耐受性或两者的转基因植物，并且已经使用包含调节植物中氮利用的核酸序列的新型载体构建体进行了转化。在各种实施方案中，载体构建体包含选自SEQ ID NO： 34,36或38.本发明还涉及用于转化植物的分离载体和用于检测转化植物的抗体。本发明还涉及在植物中表达对应于调节植物中氮利用或由氮条件调节的核酸序列的核酸分子的方法。

4. 发明申请

US20100162433A1 Plants with improved nitrogen utilization and stress tolerance 审中-公开
标题翻译：具有改善氮利用和胁迫耐受性的植物
公开(公告)号：US20100162433A1
公开(公告)日：2010-06-24
申请号：US11977768
申请日：2007-10-26
申请人： James McLaren , Nicholas Duck , Brian Vande Berg , Alissa Schawalder , Vadim Beilinson , Jill Hinson
发明人： James McLaren , Nicholas Duck , Brian Vande Berg , Alissa Schawalder , Vadim Beilinson , Jill Hinson
IPC分类号： A01H5/00 , C12N15/82 , C12N15/74 , C07K16/00 , C12N1/21 , C12N5/10
CPC分类号： C12N15/8271 , C12N15/8216 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, or both and that have been transformed using a novel vector construct including nucleic acid sequences that modulate nitrogen use in plants. In various embodiments, the vector construct includes one or more nucleic acid sequences selected from the group consisting of SEQ ID NO: 2, 4, 7, 9, 11, 13, 15, 17, 22, 24, 26, 28, 30, 32, 34, 36, or 38. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.
摘要翻译：本发明涉及具有增加的氮利用效率，胁迫耐受性或两者的转基因植物，并且已经使用包含调节植物中氮利用的核酸序列的新型载体构建体进行了转化。在各种实施方案中，载体构建体包含一个或多个选自SEQ ID NO： 32,34,36或38.本发明还涉及用于转化植物的分离载体和用于检测转化植物的抗体。本发明还涉及在植物中表达对应于调节植物中氮利用或由氮条件调节的核酸序列的核酸分子的方法。

5. 发明申请

US20080070829A1 FAMILY OF PESTICIDAL PROTEINS AND METHODS FOR THEIR USE 审中-公开
标题翻译：杀虫蛋白家族及其使用方法
公开(公告)号：US20080070829A1
公开(公告)日：2008-03-20
申请号：US11763947
申请日：2007-06-15
申请人： Nadine Carozzi , Michael Koziel , Nicholas Duck , Nalini Desai , Rong Guo , Daniel Tomso , Rebekah Deter , Tracy Hargiss
发明人： Nadine Carozzi , Michael Koziel , Nicholas Duck , Nalini Desai , Rong Guo , Daniel Tomso , Rebekah Deter , Tracy Hargiss
IPC分类号： A01N37/18 , A01H1/00 , A01H5/00 , A01P5/00 , C12N1/00 , C12N15/63 , C12P21/00 , C12N5/04 , C12N1/21 , C07H21/00 , A01P15/00 , A01H5/10
CPC分类号： C12N15/8286 , A01N37/44 , C07K14/325 , C12P21/02 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. NRRL B-30961, B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-50047, B-30959, B-30960, B-30943, B-50048, or B-50048, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 25，27，29，31，33，35，37或61所述的核苷酸序列，所述核苷酸序列如SEQ ID NO：1,3,5,8,10,12,14,16,18,20,22,24， 26,28,30,32,34,36或60个核苷酸序列，或作为保藏号NRRL B-30961，B-30955，B-30956，B-30957，B-30958，B -30942，B-30939，B-30941，B-50047，B-50047，B-30959，B-30960，B-30943，B-50048或B-50048，以及其变体和片段。

6. 发明申请

US20140344999A1 FAMILY OF PESTICIDAL PROTEINS AND METHODS FOR THEIR USE 审中-公开
标题翻译：杀虫蛋白家族及其使用方法
公开(公告)号：US20140344999A1
公开(公告)日：2014-11-20
申请号：US14451659
申请日：2014-08-05
申请人： Nadine Carozzi , Michael G. Koziel , Nicholas Duck , Nalini M. Desai , Rong Guo , Daniel John Tomso , Rebekah Deter , Tracy Hargiss
发明人： Nadine Carozzi , Michael G. Koziel , Nicholas Duck , Nalini M. Desai , Rong Guo , Daniel John Tomso , Rebekah Deter , Tracy Hargiss
IPC分类号： C12N15/82 , C07K14/325 , C12P21/02 , A01N37/44
CPC分类号： C12N15/8286 , A01N37/44 , C07K14/325 , C12P21/02 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO: 2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-30959, B-30960, B-30943, or B-50048, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 25，27，29，31，33，35，37或61所述的核苷酸序列，所述核苷酸序列如SEQ ID NO：1,3,5,8,10,12,14,16,18,20,22,24， 26,28,30,32,34,36或60个核苷酸序列，或作为保藏号B-30955，B-30956，B-30957，B-30958，B-30942，B- 30939，B-30941，B-50047，B-30959，B-30960，B-30943或B-50048，以及其变体和片段。

7. 发明申请

US20090181116A1 AXMI-031, AXMI-039, AXMI-040 AND AXMI-049, A FAMILY OF NOVEL DELTA-ENDOTOXIN GENES AND METHODS FOR THEIR USE 有权
标题翻译： AXMI-031，AXMI-039，AXMI-040和AXMI-049，新型内啡肽基因家族及其使用方法
公开(公告)号：US20090181116A1
公开(公告)日：2009-07-16
申请号：US12364335
申请日：2009-02-02
申请人： Nadine Carozzi , Nicholas Duck , Theodore Kahn , Nalini Desai , Shruti Agarwal , Daniel John Tomso , Rong Guo , Julia Daum
发明人： Nadine Carozzi , Nicholas Duck , Theodore Kahn , Nalini Desai , Shruti Agarwal , Daniel John Tomso , Rong Guo , Julia Daum
IPC分类号： A01N63/02 , C07K14/325 , C07K16/12 , C12P21/02 , A01N33/08 , A01P7/04
CPC分类号： C07K14/325 , C12N15/8285 , C12N15/8286 , Y02A40/162 , Y02A40/164
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 8, 10, 12, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, or 38, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 7, 9, 11, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, or 37, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。具体地说，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 27,29,31,33,35或38所示的核苷酸序列，或SEQ ID NO：1,3,5,7,9,11,14,16,18,20,22,24,26， 28，30，32，34或37，以及其变体和片段。

8. 发明申请

US20070294787A1 AXMI-031, AXMI-039, AXMI-040 AND AXMI-049, A FAMILY OF NOVEL DELTA ENDOTOXIN GENES AND METHODS FOR THEIR USE 有权
标题翻译： AXMI-031，AXMI-039，AXMI-040和AXMI-049，新型内啡肽基因的家族及其使用方法
公开(公告)号：US20070294787A1
公开(公告)日：2007-12-20
申请号：US11762886
申请日：2007-06-14
申请人： Nadine Carozzi , Nicholas Duck , Theodore Kahn , Nalini Desai , Shruti Jain , Daniel Tomso , Rong Guo , Julia Thissen
发明人： Nadine Carozzi , Nicholas Duck , Theodore Kahn , Nalini Desai , Shruti Jain , Daniel Tomso , Rong Guo , Julia Thissen
IPC分类号： A01H1/00 , C07H21/04 , C12N15/82 , A01N65/00 , C12N5/04
CPC分类号： C07K14/325 , C12N15/8285 , C12N15/8286 , Y02A40/162 , Y02A40/164
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 8, 10, 12, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, or 38, or the nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, or 37, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。具体地说，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 27,29,31,33,35或38所示的核苷酸序列，或SEQ ID NO：1,3,5,7,9,11,14,16,18,20,22,24,26， 28，30，32，34或37，以及其变体和片段。

9. 发明授权

US08147856B2 AXMI-031, AXMI-039, AXMI-040 and AXMI-049, a family of novel delta-endotoxin genes and methods for their use 有权
标题翻译： AXMI-031，AXMI-039，AXMI-040和AXMI-049，一种新型的δ-内毒素基因及其使用方法
公开(公告)号：US08147856B2
公开(公告)日：2012-04-03
申请号：US12364335
申请日：2009-02-02
申请人： Nadine Carozzi , Nicholas Duck , Theodore Kahn , Nalini Desai , Shruti Agarwal , Daniel John Tomso , Rong Guo , Julia Daum
发明人： Nadine Carozzi , Nicholas Duck , Theodore Kahn , Nalini Desai , Shruti Agarwal , Daniel John Tomso , Rong Guo , Julia Daum
IPC分类号： A01N63/02 , C12N15/32 , C12P21/04 , C07K14/325
CPC分类号： C07K14/325 , C12N15/8285 , C12N15/8286 , Y02A40/162 , Y02A40/164
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 8, 10, 12, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, or 38, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 7, 9, 11, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, or 37, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。具体地说，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 27,29,31,33,35或38所示的核苷酸序列，或SEQ ID NO：1,3,5,7,9,11,14,16,18,20,22,24,26， 28，30，32，34或37，以及其变体和片段。

10. 发明授权

US08829279B2 Family of pesticidal proteins and methods for their use 有权
标题翻译：一系列杀虫蛋白及其使用方法
公开(公告)号：US08829279B2
公开(公告)日：2014-09-09
申请号：US13168454
申请日：2011-06-24
申请人： Nadine Carozzi , Michael G. Koziel , Nicholas Duck , Nalini M. Desai , Rong Guo , Daniel John Tomso , Rebekah Deter , Tracy Hargiss
发明人： Nadine Carozzi , Michael G. Koziel , Nicholas Duck , Nalini M. Desai , Rong Guo , Daniel John Tomso , Rebekah Deter , Tracy Hargiss
IPC分类号： A01H5/00 , C12N15/82 , C07K14/325 , A01N37/18
CPC分类号： C12N15/8286 , A01N37/44 , C07K14/325 , C12P21/02 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. NRRL B-30961, B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-30959, B-30960, B-30943, or B-50048, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 25，27，29，31，33，35，37或61所述的核苷酸序列，所述核苷酸序列如SEQ ID NO：1,3,5,8,10,12,14,16,18,20,22,24， 26,28,30,32,34,36或60个核苷酸序列，或作为保藏号NRRL B-30961，B-30955，B-30956，B-30957，B-30958，B -30942，B-30939，B-30941，B-50047，B-30959，B-30960，B-30943或B-50048，以及其变体和片段。

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