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1. 发明授权

US11186810B2 Cell culture vessel 有权
公开(公告)号：US11186810B2
公开(公告)日：2021-11-30
申请号：US16084283
申请日：2016-03-16
申请人： Shimadzu Corporation , Yokohama City University , Osaka University , CORNING INCORPORATED
发明人： Tomoki Ohkubo , Masaki Kanai , Hirohisa Abe , Hideki Taniguchi , Masahiro Kinooka , Goro Kobayashi , Takanori Takebe
IPC分类号： C12M1/00 , C12M3/00 , C12M3/06 , C12M1/34
摘要： A cell culture container includes a storage container, a cell culture area, a culture solution supply unit, and a discharge unit. The cell culture area is a region for culturing cells, and is provided in the storage container. The culture solution supply unit supplies the culture solution into the cell culture area at a flow rate equal to or less than a predetermined flow rate. The discharge unit discharges the liquid in the cell culture area to the outside of the storage container. A rectification unit is a structure in which a plurality of outflow ports are uniformly arranged in a direction perpendicular to the flow direction of the culture solution from the culture solution supply unit to the discharge unit.

2. 发明申请

US20230035766A1 CELL CULTURE EVALUATION SYSTEM 有权
公开(公告)号：US20230035766A1
公开(公告)日：2023-02-02
申请号：US17788269
申请日：2020-12-21
申请人： Nihon Kohden Corporation , Osaka University
发明人： Hodaka Makino , Hirotsugu Kubo , Masahiro Kinooka
IPC分类号： C12M1/34 , C12M1/36
摘要： The presently disclosed subject matter provides a cell culture evaluation system including a measurement unit, a first storage unit, a second storage unit, an evaluation unit and an output unit, wherein: the measurement unit includes a sensor that acquires evaluation target data about motion or a state of a culture tool; the first storage unit stores the evaluation target data; the second storage unit stores reference data; the evaluation unit compares the evaluation target data with the reference data so as to evaluate an execution state of a cell culture process; and the output unit outputs an evaluation result created by the evaluation unit.

3. 发明授权

US09822344B2 Method for culturing pluripotent stem cells 有权
公开(公告)号：US09822344B2
公开(公告)日：2017-11-21
申请号：US14655804
申请日：2013-12-26
申请人： OSAKA UNIVERSITY
发明人： Masahiro Kinooka , Meehae Kim , Yukako Fujinaga , Yo Sugawara
IPC分类号： C12N5/074
CPC分类号： C12N5/0696 , C12N2501/58 , C12N2501/734 , C12N2501/998
摘要： Provided is a method by which cells deviated from the undifferentiated state, which emerge in a colony during culture of stem cells having pluripotency, can be removed. In one aspect, provided is a method for culturing stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion. In another aspect, provided is a method for removing cells deviated from the undifferentiated state, the cells being cells that have emerged or may possibly emerge during culture of stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion. In still another aspect, provided is a method for maintaining the undifferentiated state of stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion.

4. 发明申请

US20170130206A1 Mutant Hemagglutinin Complex Protein, and Method for Culturing Pluripotent Stem Cells Using Same 审中-公开
公开(公告)号：US20170130206A1
公开(公告)日：2017-05-11
申请号：US15321835
申请日：2015-06-29
申请人： Osaka University
发明人： Masahiro Kinooka , Meehae Kim , Yukako Fujinaga , Yo Sugawara
IPC分类号： C12N5/074 , C12N5/00 , C07K14/33
CPC分类号： C12N5/0696 , C07K14/33 , C07K19/00 , C12N1/00 , C12N5/0081 , C12N5/10 , C12N15/09 , C12N2501/998
摘要： Provided are a novel hemagglutinin that can remove cells deviated from the undifferentiated state, the cells being cells that emerge in a colony during culture of stem cells having pluripotency, and a novel method for culturing stem cells having pluripotency, the method using hemagglutinin. Provided is a mutant hemagglutinin complex protein derived from Clostridium botulinum type B, the complex protein containing at least subcomponents HA2 and HA3 of hemagglutinin derived from Clostridium botulinum type B, and the complex protein containing amino acids constituting a glycosylation site, with at least one of the amino acids having been mutated. Provided is a method for culturing stem cells having pluripotency, the method including culturing the stem cell having pluriopotency in the presence of a mutant hemagglutinin complex protein of the present disclosure.

5. 发明授权

US11505774B2 Sample storage apparatus 有权
公开(公告)号：US11505774B2
公开(公告)日：2022-11-22
申请号：US16659825
申请日：2019-10-22
申请人： NIHON KOHDEN CORPORATION , OSAKA UNIVERSITY
发明人： Hodaka Makino , Hirotsugu Kubo , Tetsuya Ogawa , Masahiro Kinooka
IPC分类号： C12M1/00 , C12M1/34 , C12M1/12
摘要： A sample storage apparatus includes: a lid member; a sample storage container; first and second fluid sucking sections; first and second fluid discharging sections; first to fourth flow paths; and a first fluid identification sensor. The first flow path is connected to the first fluid sucking section, the second flow path is connected to the second fluid sucking section, the third flow path is connected to the first fluid discharging section, and the fourth flow path is connected to the second fluid discharging section. The first fluid identification sensor for identifying a fluid is disposed in the first flow path.

6. 发明授权

US11230701B2 Mutant hemagglutinin complex protein, and method for culturing pluripotent stem cells using same 有权
公开(公告)号：US11230701B2
公开(公告)日：2022-01-25
申请号：US15321835
申请日：2015-06-29
申请人： Osaka University
发明人： Masahiro Kinooka , Meehae Kim , Yukako Fujinaga , Yo Sugawara
IPC分类号： C12N5/074 , C07K14/33 , C07K19/00 , C12N15/09 , C12N5/10 , C12N1/00 , C12N5/00
摘要： Provided are a novel hemagglutinin that can remove cells deviated from the undifferentiated state, the cells being cells that emerge in a colony during culture of stem cells having pluripotency, and a novel method for culturing stem cells having pluripotency, the method using hemagglutinin. Provided is a mutant hemagglutinin complex protein derived from Clostridium botulinum type B, the complex protein containing at least subcomponents HA2 and HA3 of hemagglutinin derived from Clostridium botulinum type B, and the complex protein containing amino acids constituting a glycosylation site, with at least one of the amino acids having been mutated. Provided is a method for culturing stem cells having pluripotency, the method including culturing the stem cell having pluriopotency in the presence of a mutant hemagglutinin complex protein of the present disclosure.

7. 发明申请

US20150329831A1 Method for Culturing Pluripotent Stem Cells 有权
标题翻译：培养多能干细胞的方法
公开(公告)号：US20150329831A1
公开(公告)日：2015-11-19
申请号：US14655804
申请日：2013-12-26
申请人： OSAKA UNIVERSITY
发明人： Masahiro Kinooka , Meehae Kim , Yukako Fujinaga , Yo Sugawara
IPC分类号： C12N5/074
CPC分类号： C12N5/0696 , C12N2501/58 , C12N2501/734 , C12N2501/998
摘要： Provided is a method by which cells deviated from the undifferentiated state, which emerge in a colony during culture of stem cells having pluripotency, can be removed. In one aspect, provided is a method for culturing stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion. In another aspect, provided is a method for removing cells deviated from the undifferentiated state, the cells being cells that have emerged or may possibly emerge during culture of stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion. In still another aspect, provided is a method for maintaining the undifferentiated state of stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion.
摘要翻译：可以除去在具有多能性的干细胞的培养期间在殖民地中出现的未分化状态的细胞偏离的方法。一方面，提供了培养具有多能性的干细胞的方法，所述方法包括在可抑制细胞粘附的物质存在下进行细胞培养。在另一方面，提供了一种去除脱离未分化状态的细胞的方法，所述细胞是在具有多能性的干细胞的培养期间已经出现或可能出现的细胞，所述方法包括在可以存在的物质存在下进行细胞培养抑制细胞粘附。另一方面，提供一种用于维持具有多能性的干细胞的未分化状态的方法，所述方法包括在可抑制细胞 - 细胞粘附的物质存在下进行细胞培养。

8. 发明申请

US20220113232A1 Particle Distribution Method 有权
公开(公告)号：US20220113232A1
公开(公告)日：2022-04-14
申请号：US17268689
申请日：2019-08-13
申请人： Osaka University
发明人： Masahiro Kinooka
IPC分类号： G01N1/38 , A01N1/02 , B01L3/02
摘要： Provided is a distribution method with which particle components such as cells can be dispensed. In one aspect, provided is a method for distributing particles, the method including dispersing the particles in a plastic fluid, and dispensing the plastic fluid containing the particles. In another aspect, provided is a method for producing a product including a particle and a container for the particle, the method including dispensing a plastic fluid in which the particles are dispersed, from a storage part in which the plastic fluid is stored, to a plurality of the containers.

9. 发明授权

US10954487B2 Cell culturing method 有权
公开(公告)号：US10954487B2
公开(公告)日：2021-03-23
申请号：US16071969
申请日：2017-01-20
申请人： Osaka University
发明人： Masahiro Kinooka , Eiji Nagamori
IPC分类号： C12N5/00 , C12M1/00 , C12N5/074 , C12M1/04 , C12M1/34 , C12N5/0735
摘要： Provided is a cell culturing method in which physical stress is reduced and oxygen can be supplied. The invention relates to a cell culturing method including; arranging cells in a dispersed state in a culture medium which is a plastic fluid; and introducing air bubbles into said culture medium.

10. 发明申请

US20200017836A1 Composition for Promoting Proliferation of Pluripotent Stem Cells, and Method for Promoting Proliferation of Pluripotent Stem Cells 审中-公开
公开(公告)号：US20200017836A1
公开(公告)日：2020-01-16
申请号：US16471312
申请日：2017-12-19
申请人： Osaka University
发明人： Masahiro Kinooka , Meehae Kim , Yukako Fujinaga
IPC分类号： C12N5/074
摘要： Provided are a method for promoting the proliferation of pluripotent stem cells and a proliferation promoting composition used in the method. In one aspect, a composition for promoting the proliferation of pluripotent stem cells includes hemagglutinin or modified hemagglutinin. In another aspect, a method for promoting the proliferation of pluripotent stem cells includes culturing pluripotent stem cells in a culture medium to which the proliferation promoting composition of the present disclosure has been added.

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