专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US09834813B2 Method for detecting mutant DNA 有权
公开(公告)号：US09834813B2
公开(公告)日：2017-12-05
申请号：US13825223
申请日：2011-09-22
申请人： Shoichi Matsukuma , Tomokazu Ishikawa
发明人： Shoichi Matsukuma , Tomokazu Ishikawa
IPC分类号： C12Q1/68 , C12P19/34 , G01N33/00 , C07H21/02 , C07H21/04 , B01D57/02 , B01D59/42 , B01D59/50 , B01D61/42 , B01D61/58
CPC分类号： C12Q1/6827 , C12Q2565/125 , C12Q2565/131
摘要： The present invention relates to a method for detecting of a mutant DNA using a probe, comprising: (1) contacting a sample containing a single-stranded DNA which has a substituted nucleotide, a deleted nucleotide region, or an inserted nucleotide region (mutant-type DNA), or/and a wild-type single-stranded DNA (wild-type DNA) corresponding thereto with the probe which hybridizes with both single-stranded DNA, to form a hybrid with the mutant-type DNA (mutant-type hybrid) or/and a hybrid with a wild-type DNA (wild-type hybrid), wherein at least one of the obtained mutant-type hybrid and wild-type hybrid has the stem structure; (2) separating the obtained mutant-type hybrid or/and wild-type hybrid by electrophoresis on the basis of presence or absence of the stem structure or difference in the stem structure; and (3) detecting the presence or absence of the mutant-type DNA in the sample.

2. 发明申请

US20130217016A1 METHOD FOR DETECTING MUTANT DNA 有权
标题翻译：检测突变体DNA的方法
公开(公告)号：US20130217016A1
公开(公告)日：2013-08-22
申请号：US13825223
申请日：2011-09-22
申请人： Shoichi Matsukuma
发明人： Shoichi Matsukuma
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2565/125 , C12Q2565/131
摘要： The present invention relates to a method for detecting of a mutant DNA using a probe, comprising: (1) contacting a sample containing a single-stranded DNA which has a substituted nucleotide, a deleted nucleotide region, or an inserted nucleotide region (mutant-type DNA), or/and a wild-type single-stranded DNA (wild-type DNA) corresponding thereto with the probe which hybridizes with both single-stranded DNA, to form a hybrid with the mutant-type DNA (mutant-type hybrid) or/and a hybrid with a wild-type DNA (wild-type hybrid), wherein at least one of the obtained mutant-type hybrid and wild-type hybrid has the stem structure; (2) separating the obtained mutant-type hybrid or/and wild-type hybrid by electrophoresis on the basis of presence or absence of the stem structure or difference in the stem structure; and (3) detecting the presence or absence of the mutant-type DNA in the sample.
摘要翻译：本发明涉及使用探针检测突变型DNA的方法，其特征在于，包括：（1）使含有具有取代核苷酸，缺失的核苷酸区域或插入的核苷酸区域的单链DNA的样品（突变型 - 类型DNA），和/或与两个单链DNA杂交的探针相应的野生型单链DNA（野生型DNA），以与突变型DNA（突变型杂合体）或/和与野生型DNA（野生型杂种）的杂交体，其中至少一种获得的突变型杂种和野生型杂种具有茎结构; （2）基于茎结构的存在或不存在或茎结构的差异通过电泳分离获得的突变型杂合体或/和野生型杂合体; 和（3）检测样品中突变型DNA的存在或不存在。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式