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1. 发明申请

US20090185734A1 Apparatus and method for analysis of particles in a liquid sample 审中-公开
标题翻译：用于分析液体样品中颗粒的装置和方法
公开(公告)号：US20090185734A1
公开(公告)日：2009-07-23
申请号：US12314838
申请日：2008-12-17
申请人： Stellan Lindberg , Tom Olesen , Martin Valvik
发明人： Stellan Lindberg , Tom Olesen , Martin Valvik
IPC分类号： G06K9/00 , G01N33/48
CPC分类号： G06K9/00134
摘要： The invention relates to a measurement apparatus, and method, for analysis of particles in a liquid sample, the apparatus comprising: an image acquiring device, an image analyser, a holder arranged to hold a sample retaining device, and a light refractor positioned between said image acquiring device and said holder; whereby at least one of the image acquiring device, the light refractor and the holder is movable by means of a piezoelectric motor, thereby moving a focus plane within the sample retaining device when it is held by the holder whereby the image acquiring device is adapted to acquire a plurality of images of said sample at different focus planes within the sample retaining device; wherein the image analyser is arranged to analyse at least one acquired image for identifying which of the particles are imaged in focus, and analysing those particles which have been identified as being imaged in focus.
摘要翻译：本发明涉及一种用于分析液体样品中的颗粒的测量装置和方法，该装置包括：图像获取装置，图像分析器，设置成保持样品保持装置的保持器和位于所述图像获取装置和所述保持器; 由此图像获取装置，光折射器和保持器中的至少一个可以通过压电电动机移动，从而当由保持器保持时移动样品保持装置内的聚焦平面，由此图像获取装置适于在样品保持装置内的不同聚焦平面处获取所述样品的多个图像; 其中所述图像分析器被布置成分析至少一个获取的图像，用于识别聚焦中的哪个颗粒成像，并且分析已经被识别为焦点成像的那些颗粒。

2. 发明授权

US08009894B2 Method and apparatus for analysis of particles in a liquid sample 有权
标题翻译：用于分析液体样品中颗粒的方法和装置
公开(公告)号：US08009894B2
公开(公告)日：2011-08-30
申请号：US12314759
申请日：2008-12-16
申请人： Stellan Lindberg , Tom Olesen , Martin Valvik
发明人： Stellan Lindberg , Tom Olesen , Martin Valvik
IPC分类号： G06K9/00
CPC分类号： G06T7/40 , B01L3/502715 , B01L2200/0647 , B01L2300/069 , G01N15/1434 , G01N15/1456 , G01N2015/1445 , G06T2207/10056
摘要： The invention relates to methods for analysis of particles in a liquid sample, the sample being retained in a sample retaining device, the method comprising: acquiring, by means of an image acquiring device, a plurality of images of said sample at different focus planes within the sample retaining device; analysing said images, by means of an image analyser, for identifying which, if any, of the particles of the sample are imaged in focus in each of the images, and analysing those particles which have been identified as being imaged in focus; wherein said plurality of images are acquired at different, essentially parallel, focus planes, which planes are separated from each other by a distance, said distance being less than 10 micrometers. The invention also relates to apparatuses adapted to be used for the inventive methods.
摘要翻译：本发明涉及用于分析液体样品中的颗粒的方法，样品保留在样品保持装置中，该方法包括：通过图像获取装置获取在不同聚焦平面内的所述样品的多个图像，样品保持装置; 通过图像分析器分析所述图像，用于识别在每个图像中哪个样本的颗粒（如果有的话）被聚焦成像，并且分析已被识别为焦点成像的那些颗粒; 其中所述多个图像在不同的基本平行的聚焦平面上获取，所述多个平面相互间隔开一定距离，所述距离小于10微米。本发明还涉及适用于本发明方法的设备。

3. 发明申请

US20090185714A1 Method and apparatus for analysis of particles in a liquid sample 有权
标题翻译：用于分析液体样品中颗粒的方法和装置
公开(公告)号：US20090185714A1
公开(公告)日：2009-07-23
申请号：US12314759
申请日：2008-12-16
申请人： Stellan Lindberg , Tom Olesen , Martin Valvik
发明人： Stellan Lindberg , Tom Olesen , Martin Valvik
IPC分类号： G06K9/00
CPC分类号： G06T7/40 , B01L3/502715 , B01L2200/0647 , B01L2300/069 , G01N15/1434 , G01N15/1456 , G01N2015/1445 , G06T2207/10056
摘要： The invention relates to methods for analysis of particles in a liquid sample, the sample being retained in a sample retaining device, the method comprising: acquiring, by means of an image acquiring device, a plurality of images of said sample at different focus planes within the sample retaining device; analysing said images, by means of an image analyser, for identifying which, if any, of the particles of the sample are imaged in focus in each of the images, and analysing those particles which have been identified as being imaged in focus; wherein said plurality of images are acquired at different, essentially parallel, focus planes, which planes are separated from each other by a distance, said distance being less than 10 micrometers. The invention also relates to apparatuses adapted to be used for the inventive methods.
摘要翻译：本发明涉及用于分析液体样品中的颗粒的方法，样品保留在样品保持装置中，该方法包括：通过图像获取装置获取在不同聚焦平面内的所述样品的多个图像，样品保持装置; 通过图像分析器分析所述图像，用于识别在每个图像中哪个样本的颗粒（如果有的话）被聚焦成像，并且分析已被识别为焦点成像的那些颗粒; 其中所述多个图像在不同的基本平行的聚焦平面上获取，所述多个平面相互间隔开一定距离，所述距离小于10微米。本发明还涉及适用于本发明方法的设备。

4. 发明申请

US20080170772A1 Apparatus for determining positions of objects contained in a sample 审中-公开
标题翻译：用于确定样品中包含的物体的位置的装置
公开(公告)号：US20080170772A1
公开(公告)日：2008-07-17
申请号：US11998416
申请日：2007-11-30
申请人： Stellan Lindberg , Tom Olesen
发明人： Stellan Lindberg , Tom Olesen
IPC分类号： G06K9/00
CPC分类号： G01N15/1463 , G01N2015/1447 , G01N2015/1486
摘要： The invention relates to an apparatus for determining positions of objects contained within a sample. The apparatus comprises an image sensor configured to transform incident light to image data, an optical system comprising a lens arrangement and an aperture, a light emitting device configured to generate light towards said image sensor, and an image data processor configured to receive image data from said image sensor and to determine positions for objects in said image data. The optical system is configured such that a depth of field of said optical system is larger than or equal to a thickness of said sample.
摘要翻译：本发明涉及一种用于确定样品中所含物体位置的装置。该装置包括被配置为将入射光转换成图像数据的图像传感器，包括透镜装置和光圈的光学系统，配置成朝向所述图像传感器产生光的发光装置，以及被配置为从所述图像传感器并且确定所述图像数据中的对象的位置。光学系统被配置为使得所述光学系统的景深大于或等于所述样品的厚度。

5. 发明授权

US08224058B2 Measurement apparatus, method and computer program 有权
标题翻译：测量装置，方法和计算机程序
公开(公告)号：US08224058B2
公开(公告)日：2012-07-17
申请号：US11822159
申请日：2007-07-02
申请人： Stellan Lindberg , Tom Olesen
发明人： Stellan Lindberg , Tom Olesen
IPC分类号： G06K9/00
CPC分类号： G01N15/1475 , G01N15/1463 , G01N2015/008 , G06K9/00134 , G06T7/0012 , G06T2207/30024
摘要： A measurement apparatus for enumeration of particles or white blood cells in a sample comprises: a holder, which is arranged to receive a sample acquiring device that holds a sample, an imaging system, comprising a magnifying means and at least one digital image acquiring means, said imaging system being arranged to acquire at least one digital image of the sample, and an image analyser, which is arranged to analyse the digital image for identifying particles or white blood cells and determining the number of particles or white blood cells and which is arranged to analyse the digital image for identifying particles or white blood cells that are imaged in focus, determining types of these particles or white blood cells, the types being distinguished by physical features and determining the ratio of different types of particles or white blood cells.
摘要翻译：用于计量样品中的颗粒或白细胞的测量装置包括：保持器，其布置成接收保持样品的样本获取装置，成像系统，包括放大装置和至少一个数字图像获取装置，所述成像系统被布置成获取所述样本的至少一个数字图像，以及图像分析器，其被配置为分析用于识别颗粒或白细胞的数字图像并且确定颗粒或白细胞的数量并且被布置分析用于鉴别聚焦成像的颗粒或白细胞的数字图像，确定这些颗粒或白细胞的类型，其类型由身体特征区分并确定不同类型的颗粒或白细胞的比例。

6. 发明申请

US20080019584A1 Measurement apparatus, method and computer program 有权
标题翻译：测量装置，方法和计算机程序
公开(公告)号：US20080019584A1
公开(公告)日：2008-01-24
申请号：US11822159
申请日：2007-07-02
申请人： Stellan Lindberg , Tom Olesen
发明人： Stellan Lindberg , Tom Olesen
IPC分类号： G06K9/00
CPC分类号： G01N15/1475 , G01N15/1463 , G01N2015/008 , G06K9/00134 , G06T7/0012 , G06T2207/30024
摘要： A measurement apparatus for enumeration of particles or white blood cells in a sample comprises: a holder, which is arranged to receive a sample acquiring device that holds a sample, an imaging system, comprising a magnifying means and at least one digital image acquiring means, said imaging system being arranged to acquire at least one digital image of the sample, and an image analyser, which is arranged to analyse the digital image for identifying particles or white blood cells and determining the number of particles or white blood cells and which is arranged to analyse the digital image for identifying particles or white blood cells that are imaged in focus, determining types of these particles or white blood cells, the types being distinguished by physical features and determining the ratio of different types of particles or white blood cells.
摘要翻译：用于计量样品中的颗粒或白细胞的测量装置包括：保持器，其布置成接收保持样品的样本获取装置，成像系统，包括放大装置和至少一个数字图像获取装置，所述成像系统被布置成获取所述样本的至少一个数字图像，以及图像分析器，其被配置为分析用于识别颗粒或白细胞的数字图像并且确定颗粒或白细胞的数量并且被布置分析用于鉴别聚焦成像的颗粒或白细胞的数字图像，确定这些颗粒或白细胞的类型，其类型由身体特征区分并确定不同类型的颗粒或白细胞的比例。

7. 发明申请

US20090153836A1 Enumeration of Thrombocytes 有权
标题翻译：血细胞计数
公开(公告)号：US20090153836A1
公开(公告)日：2009-06-18
申请号：US12227870
申请日：2007-07-04
申请人： Stellan Lindberg
发明人： Stellan Lindberg
IPC分类号： G01N1/30 , G01N33/48 , G06K9/00
CPC分类号： G01N33/58 , B01L3/5027 , G01N15/1463 , G01N33/5044 , G01N2015/0069 , G01N2015/0084 , G01N2015/1465 , G01N2015/1486
摘要： A sample acquiring device for volumetric enumeration of thrombocytes in a blood sample is provided which comprises a measurement cavity for receiving a blood sample. The measurement cavity has a predetermined fixed thickness. The sample acquiring device further comprises a reagent, which is arranged in a dried form on a surface defining the measurement cavity. The reagent comprises a haemolysing agent for lysing red blood cells in the blood sample, and optionally a staining agent for selectively staining thrombocytes in the blood sample. A system comprises the sample acquiring device and a measurement apparatus. The measurement apparatus comprises a sample acquiring device holder, a light source, and an imaging system for acquiring a digital image of a magnification of the sample. The measurement apparatus further comprises an image analyser arranged to analyse the acquired digital image for determining the number of thrombocytes in the blood sample.
摘要翻译：提供了一种用于在血液样本中体积计数血细胞的样本获取装置，其包括用于接收血液样本的测量腔。测量空腔具有预定的固定厚度。样品获取装置还包括在限定测量空腔的表面上以干燥形式布置的试剂。该试剂包括用于裂解血液样品中的红细胞的溶血剂，以及任选的用于选择性染色血液样品中的凝血细胞的染色剂。系统包括样本获取装置和测量装置。测量装置包括样本获取装置保持器，光源和用于获取样本的放大倍数的数字图像的成像系统。该测量装置还包括一个图像分析器，被配置为分析所获取的数字图像以确定血液样本中血小板数量。

8. 发明授权

US07521243B2 Enumeration of white blood cells 有权
标题翻译：计数白细胞
公开(公告)号：US07521243B2
公开(公告)日：2009-04-21
申请号：US11102837
申请日：2005-04-11
申请人： Stellan Lindberg , Johnny Svensson
发明人： Stellan Lindberg , Johnny Svensson
IPC分类号： G01N31/00
CPC分类号： G01N21/03 , A61B5/150022 , A61B5/15003 , A61B5/150099 , A61B5/150229 , A61B5/150343 , A61B5/150755 , A61B5/157 , G01N15/1475 , G01N15/1484 , G01N21/11 , G01N2015/1486 , G01N2021/0321 , G01N2021/0325 , G01N2021/0346 , Y10T436/101666 , Y10T436/107497 , Y10T436/13
摘要： A sample acquiring device for volumetric enumeration of white blood cells in a blood sample that includes a measurement cavity for receiving a blood sample. The measurement cavity has a predetermined fixed thickness. The sample acquiring device also has a reagent, which is arranged in a dried form on a surface defining the measurement cavity. The reagent has a hemolysing agent for lysing red blood cells in the sample and a staining agent for selectively staining white blood cells in the sample. Also, a system with the sample acquiring device and a measurement apparatus. The measurement apparatus has a sample acquiring device holder, a light source, and an imaging system for acquiring a digital image of a magnification of the sample The measurement apparatus also has an image analyzer arranged to analyze the acquired digital image for determining the number of white blood cells in the blood sample.
摘要翻译：一种用于在血液样本中体积计数白细胞的样本获取装置，其包括用于接收血液样本的测量腔。测量空腔具有预定的固定厚度。样品获取装置还具有在限定测量空腔的表面上以干燥形式布置的试剂。该试剂具有用于裂解样品中红细胞的溶血剂和用于选择性染色样品中白细胞的染色剂。而且，具有采样装置和测量装置的系统。测量装置具有取样装置保持器，光源以及用于获取样本的倍数的数字图像的摄像系统。测量装置还具有图像分析装置，被配置为分析所获取的数字图像以确定白色的数量血液样本中的血细胞。

9. 发明申请

US20080160566A1 Method, Device and System for Volumetric Enumeration of White Blood Cells 有权
标题翻译：白细胞体积计数的方法，装置和系统
公开(公告)号：US20080160566A1
公开(公告)日：2008-07-03
申请号：US11885637
申请日：2006-03-10
申请人： Stellan Lindberg , Johnny Svensson
发明人： Stellan Lindberg , Johnny Svensson
IPC分类号： C12M1/34 , C12Q1/20 , G06K9/00
CPC分类号： G01N21/03 , A61B5/150022 , A61B5/15003 , A61B5/150099 , A61B5/150229 , A61B5/150343 , A61B5/150755 , A61B5/157 , G01N15/1475 , G01N15/1484 , G01N21/11 , G01N2015/1486 , G01N2021/0321 , G01N2021/0325 , G01N2021/0346 , Y10T436/101666 , Y10T436/107497 , Y10T436/13
摘要： A sample acquiring device for volumetric enumeration of white blood cells in a blood sample comprises a measurement cavity for receiving a blood sample. The measurement cavity has a predetermined fixed thickness. The sample acquiring device further comprises a reagent, which is arranged in a dried form on a surface defining the measurement cavity. The reagent comprises a hemolysing agent for lysing red blood cells in the blood sample, and a staining agent for selectively staining white blood cells in the blood sample. A system comprises the sample acquiring device and a measurement apparatus. The measurement apparatus comprises a sample acquiring device holder, a light source, and an imaging system for acquiring a digital image of a magnification of the sample. The measurement apparatus further comprises an image analyser arranged to analyse the acquired digital image for determining the number of white blood cells in the blood sample.
摘要翻译：用于在血液样本中体积计数白细胞的样本获取装置包括用于接收血液样本的测量腔。测量空腔具有预定的固定厚度。样品获取装置还包括在限定测量空腔的表面上以干燥形式布置的试剂。该试剂包含用于裂解血液样品中的红细胞的溶血剂，以及用于选择性地染色血液样品中的白细胞的染色剂。系统包括样本获取装置和测量装置。测量装置包括样本获取装置保持器，光源和用于获取样本的放大倍数的数字图像的成像系统。测量装置还包括一个图像分析器，被配置为分析所获取的数字图像以确定血液样本中的白细胞数。

10. 发明授权

US08092758B2 Method, device and system for volumetric enumeration of white blood cells 有权
标题翻译：白细胞体积计数的方法，装置和系统
公开(公告)号：US08092758B2
公开(公告)日：2012-01-10
申请号：US11885637
申请日：2006-03-10
申请人： Stellan Lindberg , Johnny Svensson
发明人： Stellan Lindberg , Johnny Svensson
IPC分类号： G01N33/52 , G01N33/00
CPC分类号： G01N21/03 , A61B5/150022 , A61B5/15003 , A61B5/150099 , A61B5/150229 , A61B5/150343 , A61B5/150755 , A61B5/157 , G01N15/1475 , G01N15/1484 , G01N21/11 , G01N2015/1486 , G01N2021/0321 , G01N2021/0325 , G01N2021/0346 , Y10T436/101666 , Y10T436/107497 , Y10T436/13
摘要： A sample acquiring device for volumetric enumeration of white blood cells in a blood sample comprises a measurement cavity for receiving a blood sample. The measurement cavity has a predetermined fixed thickness. The sample acquiring device further comprises a reagent, which is arranged in a dried form on a surface defining the measurement cavity. The reagent comprises a hemolysing agent for lysing red blood cells in the blood sample, and a staining agent for selectively staining white blood cells in the blood sample. A system comprises the sample acquiring device and a measurement apparatus. The measurement apparatus comprises a sample acquiring device holder, a light source, and an imaging system for acquiring a digital image of a magnification of the sample. The measurement apparatus further comprises an image analyser arranged to analyse the acquired digital image for determining the number of white blood cells in the blood sample.
摘要翻译：用于在血液样本中体积计数白细胞的样本获取装置包括用于接收血液样本的测量腔。测量空腔具有预定的固定厚度。样品获取装置还包括在限定测量空腔的表面上以干燥形式布置的试剂。该试剂包含用于裂解血液样品中的红细胞的溶血剂，以及用于选择性地染色血液样品中的白细胞的染色剂。系统包括样本获取装置和测量装置。测量装置包括样本获取装置保持器，光源和用于获取样本的放大倍数的数字图像的成像系统。测量装置还包括一个图像分析器，被配置为分析所获取的数字图像以确定血液样本中的白细胞数。

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