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1. 发明申请

US20190240290A1 COMPANION DIAGNOSTIC FOR COMBINATION LENALIDOMIDE AND ERYTHROPOIETIN TREATMENT 审中-公开
公开(公告)号：US20190240290A1
公开(公告)日：2019-08-08
申请号：US16318512
申请日：2017-08-04
申请人： H. Lee Moffitt Cancer Center and Research Institute, Inc.
发明人： Alan F. List
IPC分类号： A61K38/18 , A61K31/454 , G01N33/569 , C12Q1/6883 , A61P7/06
CPC分类号： A61K38/1816 , A61K31/454 , A61P7/06 , C12Q1/6883 , C12Q2600/106 , C12Q2600/158 , G01N33/56966 , G01N33/57407 , G01N2333/70582 , G01N2333/70589 , G01N2800/52 , A61K2300/00
摘要： Disclosed herein is a companion diagnostic to predict efficacy of combination lenalidomide and erythropoietin treatment in patients with a erythropoietin (Epo)-refractory, Lower Risk (LR) Non-deletion 5q [Del(5q)] myelodysplasia syndrome (MDS). The method involves assaying erythroid precursors from a biological sample from the subject for a CD45 isoform profile, and treating the subject with a combination of lenalidomide and erythropoietin if the erythroid precursors have a predominance of large CD45RA and CD45RB isoforms compared to small CD45RO isoform.

2. 发明授权

US08889369B2 Method for the enumeration of mammalian micronucleated erythrocyte populations, while distinguishing platelets and/or platelet-associated aggregates 有权
标题翻译：计算哺乳动物微核红细胞群体的方法，同时区分血小板和/或血小板相关聚集体
公开(公告)号：US08889369B2
公开(公告)日：2014-11-18
申请号：US14075082
申请日：2013-11-08
申请人： Litron Laboratories, Ltd.
发明人： Stephen D. Dertinger
IPC分类号： G01N33/00 , G01N33/50 , G01N33/80
CPC分类号： G01N33/56966 , G01N33/5094 , G01N33/80 , G01N2333/70582 , G01N2333/70596 , Y02A50/58 , Y10S435/968 , Y10S435/973 , Y10T436/101666
摘要： A method for the enumeration of micronucleated erythrocyte populations while distinguishing platelet and platelet-associated aggregates involves the use of a first fluorescent labeled antibody having binding specificity for a surface marker for reticulocytes, a second fluorescent labeled antibody having binding specificity for a surface marker for platelets, and a nucleic acid staining dye that stains DNA (micronuclei) in erythrocyte populations. Because the fluorescent emission spectra of the first and second fluorescent labeled antibodies do not substantially overlap with one another or with the emission spectra of the nucleic acid staining dye, upon excitation of the labels and dye it is possible to detect the fluorescent emission and light scatter produced by the erythrocyte populations and platelets, and count the number of cells from one or more erythrocyte populations in said sample. In particular, the use of the second antibody prevents interference by platelet-associated aggregates in the scoring procedures.
摘要翻译：用于计数微核红细胞群体同时区分血小板和血小板相关聚集体的方法涉及使用具有对网织红细胞表面标志物具有结合特异性的第一荧光标记抗体，对于血小板表面标志物具有结合特异性的第二荧光标记抗体，以及在红细胞群体中染色DNA（微核）的核酸染色染料。因为第一和第二荧光标记的抗体的荧光发射光谱基本上彼此不重叠或者与核酸染色染料的发射光谱基本重叠，所以在标记和染料激发时，可以检测荧光发射和光散射由红细胞群体和血小板产生，并计数所述样品中一种或多种红细胞群体的细胞数。特别地，使用第二抗体可防止血小板相关聚集体在评分程序中的干扰。

3. 发明申请

US20130260459A1 Methods for Preserving Target Cells 审中-公开
标题翻译：保存靶细胞的方法
公开(公告)号：US20130260459A1
公开(公告)日：2013-10-03
申请号：US13896234
申请日：2013-05-16
申请人： Michael Coleman , Jody Vykoukal
发明人： Michael Coleman , Jody Vykoukal
IPC分类号： C12N5/09 , C12N5/071
CPC分类号： C12N5/0693 , A01N1/0278 , C12N5/0667 , G01N1/40 , G01N1/42 , G01N33/5047 , G01N2333/42 , G01N2333/70582 , G01N2333/70589 , G01N2333/70596
摘要： Methods for obtaining and preserving target cells using degradable three dimensional matrices are described.
摘要翻译：描述了使用可降解三维矩阵获得和保存靶细胞的方法。

4. 发明申请

US20120107803A1 METHOD FOR ENUMERATION OF MAMMALIAN MICRONUCLEATED ERYTHROCYTE POPULATIONS, WHILE DISTINGUISHING PLATELETS AND/OR PLATELET-ASSOCIATED AGGREGATES 有权
标题翻译：用于产生MAMMALIAN微核心脏细胞群体的方法，在分解平板和/或平板相关聚集体
公开(公告)号：US20120107803A1
公开(公告)日：2012-05-03
申请号：US13289764
申请日：2011-11-04
申请人： Stephen D. Dertinger
发明人： Stephen D. Dertinger
IPC分类号： C12Q1/68
CPC分类号： G01N33/56966 , G01N33/5094 , G01N33/80 , G01N2333/70582 , G01N2333/70596 , Y02A50/58 , Y10S435/968 , Y10S435/973 , Y10T436/101666
摘要： A method for the enumeration of micronucleated erythrocyte populations while distinguishing platelet and platelet-associated aggregates involves the use of a first fluorescent labeled antibody having binding specificity for a surface marker for reticulocytes, a second fluorescent labeled antibody having binding specificity for a surface marker for platelets, and a nucleic acid staining dye that stains DNA (micronuclei) in erythrocyte populations. Because the fluorescent emission spectra of the first and second fluorescent labeled antibodies do not substantially overlap with one another or with the emission spectra of the nucleic acid staining dye, upon excitation of the labels and dye it is possible to detect the fluorescent emission and light scatter produced by the erythrocyte populations and platelets, and count the number of cells from one or more erythrocyte populations in said sample. In particular, the use of the second antibody prevents interference by platelet-associated aggregates in the scoring procedures.
摘要翻译：用于计数微核红细胞群体同时区分血小板和血小板相关聚集体的方法涉及使用具有对网织红细胞表面标志物具有结合特异性的第一荧光标记抗体，对于血小板表面标志物具有结合特异性的第二荧光标记抗体，以及在红细胞群体中染色DNA（微核）的核酸染色染料。因为第一和第二荧光标记的抗体的荧光发射光谱基本上彼此不重叠或者与核酸染色染料的发射光谱基本重叠，所以在标记和染料激发时，可以检测荧光发射和光散射由红细胞群体和血小板产生，并计数所述样品中一种或多种红细胞群体的细胞数。特别地，使用第二抗体可防止血小板相关聚集体在评分程序中的干扰。

5. 发明申请

US20110076688A1 METHOD FOR ENUMERATION OF MAMMALIAN MICRONUCLEATED ERYTHROCYTE POPULATIONS, WHILE DISTINGUISHING PLATELETS AND/OR PLATELET-ASSOCIATED AGGREGATES 有权
标题翻译：用于产生MAMMALIAN微核心脏细胞群体的方法，在分解平板和/或平板相关聚集体
公开(公告)号：US20110076688A1
公开(公告)日：2011-03-31
申请号：US12962788
申请日：2010-12-08
申请人： Stephen D. Dertinger
发明人： Stephen D. Dertinger
IPC分类号： C12Q1/68
CPC分类号： G01N33/56966 , G01N33/5094 , G01N33/80 , G01N2333/70582 , G01N2333/70596 , Y02A50/58 , Y10S435/968 , Y10S435/973 , Y10T436/101666
摘要： A method for the enumeration of micronucleated erythrocyte populations while distinguishing platelet and platelet-associated aggregates involves the use of a first fluorescent labeled antibody having binding specificity for a surface marker for reticulocytes, a second fluorescent labeled antibody having binding specificity for a surface marker for platelets, and a nucleic acid staining dye that stains DNA (micronuclei) in erythrocyte populations. Because the fluorescent emission spectra of the first and second fluorescent labeled antibodies do not substantially overlap with one another or with the emission spectra of the nucleic acid staining dye, upon excitation of the labels and dye it is possible to detect the fluorescent emission and light scatter produced by the erythrocyte populations and platelets, and count the number of cells from one or more erythrocyte populations in said sample. In particular, the use of the second antibody prevents interference by platelet-associated aggregates in the scoring procedures.
摘要翻译：用于计数微核红细胞群体同时区分血小板和血小板相关聚集体的方法涉及使用具有对网织红细胞表面标志物具有结合特异性的第一荧光标记抗体，对于血小板表面标志物具有结合特异性的第二荧光标记抗体，以及在红细胞群体中染色DNA（微核）的核酸染色染料。因为第一和第二荧光标记的抗体的荧光发射光谱基本上彼此不重叠或者与核酸染色染料的发射光谱基本重叠，所以在标记和染料激发时，可以检测荧光发射和光散射由红细胞群体和血小板产生，并计数所述样品中一种或多种红细胞群体的细胞数。特别地，使用第二抗体可防止血小板相关聚集体在评分程序中的干扰。

6. 发明申请

US20080293072A1 METHOD FOR ENUMERATION OF MAMMALIAN MICRONUCLEATED ERYTHROCYTE POPULATIONS, WHILE DISTINGUISHING PLATELETS AND/OR PLATELET-ASSOCIATED AGGREGATES 有权
标题翻译：用于产生MAMMALIAN微核心脏细胞群体的方法，在分解平板和/或平板相关聚集体
公开(公告)号：US20080293072A1
公开(公告)日：2008-11-27
申请号：US12177352
申请日：2008-07-22
申请人： Stephen D. Dertinger
发明人： Stephen D. Dertinger
IPC分类号： C12Q1/68
CPC分类号： G01N33/56966 , G01N33/5094 , G01N33/80 , G01N2333/70582 , G01N2333/70596 , Y02A50/58 , Y10S435/968 , Y10S435/973 , Y10T436/101666
摘要： A method for the enumeration of micronucleated erythrocyte populations while distinguishing platelet and platelet-associated aggregates involves the use of a first fluorescent labeled antibody having binding specificity for a surface marker for reticulocytes, a second fluorescent labeled antibody having binding specificity for a surface marker for platelets, and a nucleic acid staining dye that stains DNA (micronuclei) in erythrocyte populations. Because the fluorescent emission spectra of the first and second fluorescent labeled antibodies do not substantially overlap with one another or with the emission spectra of the nucleic acid staining dye, upon excitation of the labels and dye it is possible to detect the fluorescent emission and light scatter produced by the erythrocyte populations and platelets, and count the number of cells from one or more erythrocyte populations in said sample. In particular, the use of the second antibody prevents interference by platelet-associated aggregates in the scoring procedures.
摘要翻译：用于计数微核红细胞群体同时区分血小板和血小板相关聚集体的方法涉及使用具有对网织红细胞表面标志物具有结合特异性的第一荧光标记抗体，对于血小板表面标志物具有结合特异性的第二荧光标记抗体，以及在红细胞群体中染色DNA（微核）的核酸染色染料。因为第一和第二荧光标记的抗体的荧光发射光谱基本上彼此不重叠或者与核酸染色染料的发射光谱基本重叠，所以在标记和染料激发时，可以检测荧光发射和光散射由红细胞群体和血小板产生，并计数所述样品中一种或多种红细胞群体的细胞数。特别地，使用第二抗体可防止血小板相关聚集体在评分程序中的干扰。

7. 发明申请

US20070274919A1 METHOD FOR MEASURING IN VIVO MUTATION FREQUENCY AT AN ENDOGENOUS GENE LOCUS 有权
标题翻译：用于在内源基因位置测量VIVO MUTATION频率的方法
公开(公告)号：US20070274919A1
公开(公告)日：2007-11-29
申请号：US11752726
申请日：2007-05-23
申请人： Stephen D. Dertinger
发明人： Stephen D. Dertinger
IPC分类号： A61K49/00 , C12Q1/00 , G01N33/567
CPC分类号： G01N33/80 , G01N2333/70557 , G01N2333/70582 , G01N2333/70596 , Y10S435/973 , Y10S436/811 , Y10T436/101666 , Y10T436/107497 , Y10T436/143333 , Y10T436/25125
摘要： The present invention relates a method for the enumeration of in vivo gene mutation. The method utilizes differential staining of GPI-anchor deficient erythrocyte populations to distinguish between wild-type and pig-a gene mutants. Quantitative analyses can be conducted on erythrocytes and/or reticulocytes, and is based upon fluorescent emission and light scatter following exposure to an excitatory light source. Counting of mutant erythrocytes or reticulcoytes relative to the number of total erythrocytes or reticulocytes can be used to assess the DNA-damaging potential of an exogenous chemical agent, the DNA-damaging potential of an exogenous physical agent, the effects of an exogenous agent which can modify endogenously-induced DNA damage, and the effects of an exogenous agent which can modify exogenously-induced DNA damage. Kits for practicing the invention are also disclosed.
摘要翻译：本发明涉及一种枚举体内基因突变的方法。该方法利用GPI锚定缺陷型红细胞群体的差异染色来区分野生型和pig-a基因突变体。可以对红细胞和/或网织红细胞进行定量分析，并且基于暴露于兴奋性光源之后的荧光发射和光散射。可以使用相对于总红细胞或网织红细胞数的突变型红细胞或网状细胞计数来评估外源性化学试剂的DNA损伤潜力，外源物理试剂的DNA损伤潜能，外源性试剂的作用改变内源性诱导的DNA损伤，以及可以改变外源诱导的DNA损伤的外源药物的作用。还公开了用于实施本发明的试剂盒。

8. 发明授权

US6156882A Antibody 4G8B4B12 失效
标题翻译：抗体4G8B4B12
公开(公告)号：US6156882A
公开(公告)日：2000-12-05
申请号：US105596
申请日：1998-06-26
申请人： Hans-Jorg Buhring , Irene Rappold
发明人： Hans-Jorg Buhring , Irene Rappold
IPC分类号： A61K38/00 , C07K14/705 , C07K16/28 , C12N15/06 , C12P21/08 , G01N33/569 , C12N9/64 , C07K16/00
CPC分类号： G01N33/56972 , C07K14/70596 , C07K16/2896 , A61K38/00 , G01N2333/70582 , G01N2333/71
摘要： The invention relates to a monoclonal antibody specifically binding to the human FLT3/FLK2 receptor protein. The invention further relates to hybridoma cells producing such an antibody, as well as to a method for generation of such hybridoma cells. The monoclonal antibody is the antibody produced and released by hybridoma cells deposited under No. DSM 2249 at the German Collection of Microorganisms and Cell Cultures (DSMZ) and designated as 4G8B4B12.
摘要翻译：本发明涉及与人FLT3 / FLK2受体蛋白特异性结合的单克隆抗体。本发明还涉及产生这种抗体的杂交瘤细胞，以及用于产生这种杂交瘤细胞的方法。单克隆抗体是由DSM 2249在德国微生物和细胞培养物收集库（DSMZ）上保藏并被指定为4G8B4B12的杂交瘤细胞产生和释放的抗体。

9. 发明申请

US20170226515A1 RNA APTAMERS AGAINST TRANSFERRIN RECEPTOR (TFR) 审中-公开
公开(公告)号：US20170226515A1
公开(公告)日：2017-08-10
申请号：US15519074
申请日：2015-10-15
申请人： City of Hope , Apterna Ltd
发明人： John J. Rossi , Sorah Yoon , Nagy Habib
IPC分类号： C12N15/115 , A61K31/7105 , G01N33/53 , G01N33/574 , A61K31/713 , A61K45/06
CPC分类号： C12N15/115 , A61K31/7105 , A61K31/713 , A61K45/06 , A61K47/549 , C07K14/70582 , C12N2310/16 , C12N2310/351 , C12N2310/3519 , C12N2320/31 , G01N33/5308 , G01N33/57492 , G01N2333/70582 , C12N2310/322 , C12N2310/3533
摘要： Provided herein, inter alia, are nucleic acid compounds capable of binding transferrin receptor on a cell and internalizing into said cell. The compositions provided herein may be useful for delivering therapeutic and diagnostic agents to a cell. Further provided are pharmaceutical compositions and methods of treatment using nucleic acid compounds provided herein.

10. 发明授权

US09285365B2 Method for the enumeration of mammalian micronucleated erythrocyte populations, while distinguishing platelets and/or platelet-associated aggregates 有权
标题翻译：计算哺乳动物微核红细胞群体的方法，同时区分血小板和/或血小板相关聚集体
公开(公告)号：US09285365B2
公开(公告)日：2016-03-15
申请号：US14523790
申请日：2014-10-24
申请人： Litron Laboratories, Ltd.
发明人： Stephen D. Dertinger
IPC分类号： G01N33/80 , G01N33/569 , G01N33/50
CPC分类号： G01N33/56966 , G01N33/5094 , G01N33/80 , G01N2333/70582 , G01N2333/70596 , Y02A50/58 , Y10S435/968 , Y10S435/973 , Y10T436/101666
摘要： A method for the enumeration of micronucleated erythrocyte populations while distinguishing platelet and platelet-associated aggregates involves the use of a first fluorescent labeled antibody having binding specificity for a surface marker for reticulocytes, a second fluorescent labeled antibody having binding specificity for a surface marker for platelets, and a nucleic acid staining dye that stains DNA (micronuclei) in erythrocyte populations. Because the fluorescent emission spectra of the first and second fluorescent labeled antibodies do not substantially overlap with one another or with the emission spectra of the nucleic acid staining dye, upon excitation of the labels and dye it is possible to detect the fluorescent emission and light scatter produced by the erythrocyte populations and platelets, and count the number of cells from one or more erythrocyte populations in said sample. In particular, the use of the second antibody prevents interference by platelet-associated aggregates in the scoring procedures.
摘要翻译：用于计数微核红细胞群体同时区分血小板和血小板相关聚集体的方法涉及使用具有对网织红细胞表面标志物具有结合特异性的第一荧光标记抗体，对于血小板表面标志物具有结合特异性的第二荧光标记抗体，以及在红细胞群体中染色DNA（微核）的核酸染色染料。因为第一和第二荧光标记的抗体的荧光发射光谱基本上彼此不重叠或者与核酸染色染料的发射光谱基本重叠，所以在标记和染料激发时，可以检测荧光发射和光散射由红细胞群体和血小板产生，并计数所述样品中一种或多种红细胞群体的细胞数。特别地，使用第二抗体可防止血小板相关聚集体在评分程序中的干扰。

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