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1. 发明授权

US10080745B2 Piericidin bacterial inhibitors 有权
公开(公告)号：US10080745B2
公开(公告)日：2018-09-25
申请号：US15039116
申请日：2014-11-24
申请人： The Regents of the University of California Santa Cruz
发明人： Victoria Auerbuch Stone , Roger G. Linington , Weng Ruh Wong , Miles C. Duncan
IPC分类号： A61K31/44 , G01N33/94 , G01N33/50
CPC分类号： A61K31/44 , A61K39/02 , C12Q1/18 , G01N33/5008 , G01N33/94 , G01N2333/24 , G01N2333/986 , G01N2560/00 , Y02A50/406 , Y02A50/473 , Y02A50/475 , Y02A50/481
摘要： Described herein are assays for identifying piericidins and piericidin compositions.

2. 发明授权

US09902989B2 Methods for the detection of beta-lactamases 有权
公开(公告)号：US09902989B2
公开(公告)日：2018-02-27
申请号：US14745722
申请日：2015-06-22
申请人： Becton, Dickinson and Company
发明人： Mei Yang-Woytowitz , Charles Yu , Timothy Wiles
IPC分类号： C12Q1/04 , C12Q1/34
CPC分类号： C12Q1/34 , C12Q1/04 , G01N2333/986
摘要： Presented herein are methods and compositions for the detection of specific beta-lactamases, including class A serine carbapenemases, metallo-beta-lactamases, AmpC beta-lactamases, and extended-spectrum beta-lactamases (ESBLs). The methods presented herein include methods that permit the detection of the presence of specific beta-lactamases in bacterial samples within as few as 2 to 10 minutes.

3. 发明授权

US09551020B2 Method of detecting at least one mechanism of resistance to carbapenems by mass spectrometry 有权
标题翻译：通过质谱法检测至少一种对碳青霉烯抗性机制的方法
公开(公告)号：US09551020B2
公开(公告)日：2017-01-24
申请号：US14111083
申请日：2012-04-20
申请人： Yannick Charretier , Jean-Philippe Charrier , Christine Franceschi , Gilles Zambardi , Tiphaine Cecchini , Elodie Degout-Charmette
发明人： Yannick Charretier , Jean-Philippe Charrier , Christine Franceschi , Gilles Zambardi , Tiphaine Cecchini , Elodie Degout-Charmette
IPC分类号： C12Q1/37 , C12Q1/04 , G01N33/68
CPC分类号： G01N33/6848 , C12Q1/04 , C12Q1/37 , G01N2333/948 , G01N2333/976 , G01N2333/986
摘要： The present invention pertains to a method of detection, by mass spectrometry, of at least one marker of at least one mechanism of resistance to at least one antimicrobial, resistance of at least one microorganism contained in a sample, characterised in that the antimicrobial is a carbapenem, and said resistance markers are proteins or peptides. Preferably, said proteins or peptides are proteins from said microorganism.
摘要翻译：本发明涉及通过质谱法检测至少一种对至少一种抗微生物抗性的机制的至少一种标记物的方法，所述至少一种抗微生物剂包含在至少一种微生物中的抗性，其特征在于所述抗微生物剂是碳青霉烯和所述抗性标记物是蛋白质或肽。优选地，所述蛋白质或肽是来自所述微生物的蛋白质。

4. 发明授权

US09476087B2 Methods and compounds for detecting beta-lactamase activity 有权
标题翻译：检测β-内酰胺酶活性的方法和化合物
公开(公告)号：US09476087B2
公开(公告)日：2016-10-25
申请号：US14326215
申请日：2014-07-08
申请人： Nanyang Technological University
发明人： Bengang Xing , Xianfeng Huang , Tingting Jiang , Rongrong Liu
IPC分类号： C12Q1/34 , C07D501/04 , C07D501/18 , C07D501/16 , C07D519/06
CPC分类号： C12Q1/34 , C07D501/04 , C07D501/16 , C07D501/18 , C07D519/06 , G01N2333/986
摘要： The present invention relates to compounds for and a method of detecting beta-lactamase activity in a sample. The sample is contacted with a nanoparticulate tag. The nanoparticulate tag comprises a metal or a combination of metals, or it comprises a nanotube of a metal, boron nitride and/or carbon. The respective metal is capable of forming one of a covalent bond, a coordinative bond and a non-covalent interaction with a thio or a seleno group. The sample is contacted with a compound of one of general formulas (I)-(III) and (VII)-(IX). At least one beta-lactam moiety of the compound is cleaved by the beta-lactamase activity in the sample. As a result a cleavage moiety Z-A-Z, Z-A-Z—R15, Z-A-Z—R16, Z-A-Z—R17, Z-A-Z—R18 or Z-G-N(R8)R9 is released that is immobilized on the surface of the nanoparticulate tag by a covalent bond via a Z atom. The presence of beta-lactamase activity is determined based on the presence of the cleavage moiety immobilized onto the surface of the nanoparticulate tag.
摘要翻译：本发明涉及用于检测样品中β-内酰胺酶活性的化合物和方法。样品与纳米颗粒标签接触。纳米颗粒标签包括金属或金属的组合，或者它包括金属，氮化硼和/或碳的纳米管。相应的金属能够形成共价键，配位键和与硫代或硒基的非共价相互作用之一。将样品与通式（I） - （III）和（VII） - （IX）之一的化合物接触。化合物的至少一个β-内酰胺部分被样品中的β-内酰胺酶活性切割。结果，通过Z原子通过共价键将解离部分Z-A-Z，Z-A-Z-R15，Z-A-Z-R16，Z-A-Z-R17，Z-A-Z-R18或Z-G-N（R8）R9释放固定在纳米颗粒标签的表面上。基于固定在纳米颗粒标签表面上的切割部分的存在来确定β-内酰胺酶活性的存在。

5. 发明授权

US08180580B2 Method for identifying a compound that modulates SIR2 protein activity 有权
标题翻译：识别调节SIR2蛋白活性的化合物的方法
公开(公告)号：US08180580B2
公开(公告)日：2012-05-15
申请号：US12023287
申请日：2008-01-31
申请人： Ronen Marmorstein , Brandi D. Sanders
发明人： Ronen Marmorstein , Brandi D. Sanders
IPC分类号： G01N31/00 , G06G7/58 , C12Q1/34 , C12N9/78
CPC分类号： C12Q1/25 , G01N2333/986 , G01N2500/04
摘要： The present invention relates to a method for identifying compounds that modulate the activity of sirtuin deacetylase protein family members. Compounds of the invention are identified by designing or screening for a compound which binds to at least one amino acid residue of the newly identified nicotinamide inhibition and base exchange site of Sir2 and testing the compound for its ability to modulate the activity of the Sir2 protein. Compositions and methods for preventing or treating diseases or disorders associated with Sir2 are also provided.
摘要翻译：本发明涉及一种鉴定调节沉默调节蛋白脱乙酰酶蛋白家族成员活性的化合物的方法。通过设计或筛选与新鉴定的Sir2的烟酰胺抑制和碱基交换位点的至少一个氨基酸残基并测试该化合物调节Sir2蛋白活性的能力的化合物来鉴定本发明的化合物。还提供了用于预防或治疗与Sir2相关的疾病或病症的组合物和方法。

6. 发明申请

US20120064557A1 METHOD OF IDENTIFYING METALLO-BETA-LACTAMASE-PRODUCING BACTERIA 失效
标题翻译：鉴定生产金属 - β-谷氨酸酶的细菌的方法
公开(公告)号：US20120064557A1
公开(公告)日：2012-03-15
申请号：US13260965
申请日：2010-03-31
申请人： Akihiro Morinaka
发明人： Akihiro Morinaka
IPC分类号： C12Q1/34
CPC分类号： C12Q1/04 , C12Q1/045 , C12Q1/34 , G01N2333/986
摘要： An object of the present invention is to provide a method of identifying metallo-β-lactamase-producing bacteria, particularly, a method of conveniently identifying an IMP or VIM type. According to the present invention, there is provided a method of detecting metallo-β-lactamase-producing bacteria, comprising spotting a compound (I) onto a surface of a solid medium coated with the bacteria to be tested, spotting 3 types of β-lactam agents at 3 respective positions different from the spot of the compound (I), culturing the solid medium, and then detecting the metallo-β-lactamase-producing bacteria based on the shape of an inhibition zone formed around the spot of each of the β-lactam agents. There is further provided a method of identifying an IMP or VIM type of the metallo-β-lactamase-producing bacteria.
摘要翻译：本发明的目的是提供一种识别产生金属 - β-内酰胺酶的细菌的方法，特别是方便地鉴定IMP或VIM型的方法。根据本发明，提供了一种检测产生金属 - β-内酰胺酶的细菌的方法，其包括将化合物（I）放在涂覆有待测试细菌的固体培养基的表面上，发现3种类型的 ;在与化合物（I）的斑点不同的3个位置处的内酰胺试剂，培养固体培养基，然后基于形成在所述化合物（I）的斑点周围的抑制区的形状来检测产生金属和β-内酰胺酶的细菌每个＆bgr。 - 内酰胺代理。还提供了鉴定产生金属 - β-内酰胺酶的细菌的IMP或VIM类型的方法。

7. 发明申请

US20120040380A1 OPTICALLY-DETECTABLE ENZYME SUBSTRATES AND THEIR METHOD OF USE 有权
标题翻译：光学检测酶基质及其使用方法
公开(公告)号：US20120040380A1
公开(公告)日：2012-02-16
申请号：US13166627
申请日：2011-06-22
申请人： SCHUYLER BOON CORRY , WILLIAM LOUIS DOWNEY , BRIAN FILANOSKI , KYLE RICHARD GEE , I. LAWRENCE GREENFIELD , JAMES DAVID HIRSCH , IAIN JOHNSON , ALEKSEY RUKAVISHNIKOV
发明人： SCHUYLER BOON CORRY , WILLIAM LOUIS DOWNEY , BRIAN FILANOSKI , KYLE RICHARD GEE , I. LAWRENCE GREENFIELD , JAMES DAVID HIRSCH , IAIN JOHNSON , ALEKSEY RUKAVISHNIKOV
IPC分类号： G01N21/64 , C09B29/36 , C12Q1/34
CPC分类号： C07F5/022 , C07D499/00 , C07D501/00 , C07D501/58 , C07D501/60 , C07D503/00 , C07D519/00 , C12Q1/34 , C12Q2334/00 , G01N33/581 , G01N2333/986
摘要： The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.
摘要翻译：本发明涉及作为酶的底物的化合物，并且在与酶反应时提供可检测的反应，例如可光学检测的反应。特别地，这些化合物可用于检测样品中β-内酰胺酶的存在。除了化合物之外，公开了用于分析样品存在β-乳糖酶的方法，例如，作为包括编码β-乳糖酶的序列的核酸序列的表达的指示剂。公开了包括所公开的化合物和另外的组分的试剂盒，例如细胞，抗体，β-乳酸酶或在测定中使用组分的说明书。

8. 发明授权

US08071761B2 Substrates for beta-lactamase and uses thereof 有权
标题翻译： β-内酰胺酶底物及其用途
公开(公告)号：US08071761B2
公开(公告)日：2011-12-06
申请号：US11606642
申请日：2006-11-29
申请人： Roger Y. Tsien , Gregor Zlokarnik
发明人： Roger Y. Tsien , Gregor Zlokarnik
IPC分类号： C07D501/14
CPC分类号： C07D501/36 , C07D501/00 , C07D505/00 , C07D519/00 , C12N9/86 , C12N15/85 , C12N2800/90 , C12N2830/002 , C12P17/184 , C12P17/188 , C12P35/00 , C12Q1/18 , C12Q1/34 , C12Q1/6897 , C12Q2334/00 , G01N2333/986 , G01N2415/00 , Y10S435/805
摘要： Substrates for β-lactamase of the general formula I in which one of X and Y is a fluorescent donor moiety and the other is a quencher (which may or may not re-emit); R′ is selected from the group consisting of H, lower (i.e., alkyl of 1 to about 5 carbon atoms) and (CH2)nOH, in which n is 0 or an integer from 1 to 5; R″ is selected from the group consisting of H, physiologically acceptable metal and ammonium cations, —CHR2OCO(CH2)nCH3, —CHR2OCOC (CH3)3, acylthiomethyl, acyloxy-alpha-benzyl, delta-butyrolactonyl, methoxycarbonyloxymethyl, phenyl, methylsulphinylmethyl, beta-morpholinoethyl, dialkylaminoethyl, acyloxyalkyl, dialkylaminocarbonyloxymethyl and aliphatic, in which R2 is selected from the group consisting of H and lower alkyl; A is selected from the group consisting of S, O, SO, SO2 and CH2; and Z′ and Z″ are linkers for the fluorescent donor and quencher moieties. Methods of assaying β-lactamase activity and monitoring expression in systems using β-lactamase as a reporter gene also are disclosed.
摘要翻译： X和Y中的一个是荧光供体部分，另一个是猝灭剂（其可以或不再发射）的通式I的β-内酰胺酶底物; R'选自H，低级（即1至约5个碳原子的烷基）和（CH 2）nOH，其中n为0或1至5的整数; -CH 2 COO（CH 2）n CH 3，-CHR 2 OCOC（CH 3）3，酰基硫代甲基，酰氧基-α-苄基，δ-丁基内酰胺，甲氧基羰氧基甲基，苯基，甲基亚磺酰基甲基， β-吗啉基乙基，二烷基氨基乙基，酰氧基烷基，二烷基氨基羰氧基甲基和脂族基，其中R2选自H和低级烷基; A选自S，O，SO，SO 2和CH 2; 和Z'和Z“是荧光供体和猝灭剂部分的接头。公开了使用β-内酰胺酶作为报道基因的系统中测定β-内酰胺酶活性和监测表达的方法。

9. 发明授权

US07867758B2 Bioluminogenic assay system for measuring beta-lactamase activity 有权
标题翻译：用于测量β-内酰胺酶活性的生物发光测定系统
公开(公告)号：US07867758B2
公开(公告)日：2011-01-11
申请号：US12060864
申请日：2008-04-01
申请人： Jianghong Rao , Min-kyung So , Hequan Yao
发明人： Jianghong Rao , Min-kyung So , Hequan Yao
IPC分类号： C12M1/00 , C12Q1/34
CPC分类号： C12Q1/66 , C12Q1/34 , G01N2333/986
摘要： A bioluminogenic assay system including: providing a bioluminogenic substrate incorporating a beta-lactam antibiotic, a bioluminescence initiating compound, and a chemical linkage joining the beta-lactam antibiotic to the bioluminescence initiating compound; exposing the bioluminogenic substrate to a beta-lactamase enzyme that catalyzes the release of the bioluminescence initiating compound from the bioluminogenic substrate; co-exposing the bioluminogenic substrate to a bioluminescence indicator reaction that employs the bioluminescence initiating compound as a substrate; and detecting a light from the bioluminescence indicator reaction as a measure of the activity of the beta-lactamase enzyme.
摘要翻译：生物发光测定系统，包括：提供掺入β-内酰胺抗生素的生物发光底物，生物发光引发化合物和将β-内酰胺抗生素与生物发光引发化合物连接的化学键; 将生物发光底物暴露于催化生物发光起始化合物从生物发光底物释放的β-内酰胺酶; 将生物发光底物共同暴露于采用生物发光引发化合物作为底物的生物发光指示剂反应; 并检测来自生物发光指示剂反应的光作为β-内酰胺酶活性的量度。

10. 发明申请

US20090117601A1 METHODS AND COMPOSITIONS FOR THE DETECTION OF BETA-LACTAMASES 有权
标题翻译：用于检测BETA-LACTAMASES的方法和组合物
公开(公告)号：US20090117601A1
公开(公告)日：2009-05-07
申请号：US12254594
申请日：2008-10-20
申请人： Mei Yang-Woytowitz , Charles Yu , Timothy Wiles
发明人： Mei Yang-Woytowitz , Charles Yu , Timothy Wiles
IPC分类号： C12Q1/34
CPC分类号： C12Q1/34 , C12Q1/04 , G01N2333/986
摘要： Presented herein are methods and compositions for the detection of specific beta-lactamases, including class A serine carbapenemases, metallo-beta-lactamases. AmpC beta-lactamases, and extended-spectrum beta-lactamases (ESBLs). The methods presented herein include methods that permit the detection of the presence of specific beta-lactamases in bacterial samples within as few as 2 to 10 minutes.
摘要翻译：本文提出了用于检测特异性β-内酰胺酶的方法和组合物，包括A类丝氨酸碳青霉烯酶，金属β-内酰胺酶。 AmpCβ-内酰胺酶和广谱β-内酰胺酶（ESBLs）。本文提供的方法包括允许在少至2至10分钟内检测细菌样品中特定β-内酰胺酶的存在的方法。

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