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    • 4. 发明授权
    • Immobilized ribonuclease and alkaline phosphatase
    • 固定核糖核酸酶和碱性磷酸酶
    • US4039382A
    • 1977-08-02
    • US678459
    • 1976-04-19
    • Minh-Nguy ThangAnnie Guissani born Trachtenberg
    • Minh-Nguy ThangAnnie Guissani born Trachtenberg
    • C12N11/12C07H21/00C12N11/14C12N11/18C12P19/34C07G7/02C12B1/00
    • C07H21/00C12N11/18C12P19/34Y10S435/918
    • An insoluble, solid matrix carrying simultaneously several different enzymatic functions, is constituted by the conjoint association by irreversible binding on a previously activated matrix support, of a nuclease selected from the group of ribonucleases A, T.sub.1, T.sub.2, U.sub.2 and an alkaline phosphatease. Free activated groups of the matrix after binding of the enzymes, are neutralized by a free amino organic base. The support is selected from among non-denaturing supports effecting the irreversible physical adsorption of the enzymes, such as supports of glass or quartz beads, highly cross-linked gels of the agarose or cellulose type. Polymers A.sub.n U.sub.OH, A.sub.n C.sub.OH, A.sub.n G.sub.OH and/or oligonucleotides U, C, A or G, of predetermined lengths, can be obtained by fractionating the polyribonucleotides by incubation with the insoluble solid matrix.
    • 携带同时几种不同酶功能的不溶性固体基质是由预先活化的基质载体,选自核糖核酸酶A,T1,T2,U2和碱性磷酸酶的核酸酶的不可逆结合的共同关联构成的。 酶结合后,自由基活化基团被游离氨基有机碱中和。 支持物选自影响酶的不可逆物理吸附的非变性支持物,例如玻璃或石英珠的支撑物,琼脂糖或纤维素类型的高度交联的凝胶。 具有预定长度的聚合物AnUOH,AnCOH,AnGOH和/或寡核苷酸U,C,A或G可以通过与不溶性固体基质孵育来分级多核糖核苷酸而获得。
    • 6. 发明授权
    • Process for preparing {62 -galactosidase
    • 制备{62-半乳糖苷酶的方法
    • US3919049A
    • 1975-11-11
    • US48389674
    • 1974-06-27
    • TOKYO TANABE CO
    • KIUCHI AKIRATANAKA YUJI
    • C12N9/38C12D13/10
    • C12Y302/01023C12N9/2471Y10S435/814Y10S435/815Y10S435/917Y10S435/918
    • An acid-active, acid-stable Beta -galactosidase is prepared from the cultured material of a strain of Aspergillus oryzae. Said enzyme is recovered, in case of the liquid culture, by means of precipitation from the cultured broth by adding organic solvent thereto, while in case of the solid culture, the enzyme is recovered from the cultured material by means of extraction using water and succeeding precipitation from the resulting aqueous solution by adding organic solvent thereto. The precipitates thus obtained in both cases may further be refined in the routine manner. The present acid-active, acid-stable Beta -galactosidase is useful for recovering or preventing of vomit or diarrhoea due to the Beta -galactosidase deficiency or lactose intolerance.
    • 从米曲霉菌株的培养物质制备酸活性,酸稳定的β-半乳糖苷酶。 在液体培养的情况下,通过加入有机溶剂从培养液中沉淀回收所述酶,而在固体培养的情况下,通过使用水萃取从培养物质中回收酶 通过向其中加入有机溶剂从所得水溶液中沉淀。 这两种情况下得到的沉淀物可以进一步以常规方式进行精制。 本发明的酸活性,酸稳定的β-半乳糖苷酶可用于恢复或预防由于β-半乳糖苷酶缺乏或乳糖不耐受引起的呕吐或腹泻。