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1. 发明授权

US07429477B2 Controlling plant pathogens with bacterial/fungal antagonist combinations 失效
标题翻译：用细菌/真菌拮抗剂组合控制植物病原体
公开(公告)号：US07429477B2
公开(公告)日：2008-09-30
申请号：US10940036
申请日：2004-09-13
申请人： Thomas D. Johnson
发明人： Thomas D. Johnson
IPC分类号： C12N1/20 , C12N1/14 , C12N1/00 , A01N63/00 , A01N63/04
CPC分类号： A01N63/04 , Y02A40/14 , Y10S435/832 , Y10S435/839 , Y10S435/945 , A01N63/00 , A01N2300/00
摘要： Fungal/bacterial antagonist combinations, a seed coated with one of the combinations and a plant protected from plant pathogens by one of the combinations. The invention is also a fungal/bacterial antagonist combination comprising a Trichoderma virens fungal antagonist and a Bacillus subtilis var. amyloliquefaciens (Bacillus amyloliquefaciens) bacterial antagonist and its use for controlling plant pathogens as a biocontrol agent, bio-pesticide or bio-fungicide. The invention also finds utility as a fungal/bacterial antagonist combination applied to the seed, stalk or leaf that results in an increase in plant yield. Control of early and late season stalk and root rot caused by fungi such as Fusarium, Phythium, Phytophthora and Penicillium in tomatoes, peppers, turf grass, soybeans, sunflower, wheat and corn is achieved.
摘要翻译：真菌/细菌拮抗剂组合，用组合之一包衣的种子和通过组合之一保护植物病原体的植物。本发明还是一种真菌/细菌拮抗剂组合物，其包含木霉真菌抗真菌剂和枯草芽孢杆菌变种。解淀粉芽孢杆菌（Bacillus amyloliquefaciens）细菌拮抗剂及其用于控制植物病原体作为生物防治剂，生物杀虫剂或生物杀真菌剂的用途。本发明还可用作施用于种子，茎或叶的真菌/细菌拮抗剂组合物，导致植物产量增加。实现了西红柿，辣椒，草坪草，大豆，向日葵，小麦和玉米等真菌如镰刀菌，腐质霉，疫霉和青霉菌引起的早期和晚期季节茎和根腐病的控制。

2. 发明授权

US07166450B2 Hyaluronan synthase gene and uses thereof 失效
标题翻译：透明质酸合酶基因及其用途
公开(公告)号：US07166450B2
公开(公告)日：2007-01-23
申请号：US11446854
申请日：2006-06-05
申请人： Paul H. Weigel , Kshama Kumari , Paul DeAngelis
发明人： Paul H. Weigel , Kshama Kumari , Paul DeAngelis
IPC分类号： C12P19/18 , C12N9/10 , C07H21/04
CPC分类号： C12P19/26 , A61K39/102 , C07H21/04 , C08B37/0072 , C12N9/1051 , C12P19/04 , Y10S435/836 , Y10S435/839 , Y10S435/848
摘要： The present invention relates to a method of producing hyaluronic acid that includes introducing a purified nucleic acid segment having a coding region encoding enzymatically active hyaluronan synthase into a Bacillus strain, and culturing the Bacillus strain under conditions appropriate for the production of hyaluronic acid.
摘要翻译：本发明涉及一种生产透明质酸的方法，其包括将具有编码酶活性透明质酸合酶编码区的纯化核酸片段导入芽孢杆菌属菌株，并在适于生产透明质酸的条件下培养芽孢杆菌菌株。

3. 发明授权

US07153677B2 Hyaluronan synthase gene and uses thereof 失效
公开(公告)号：US07153677B2
公开(公告)日：2006-12-26
申请号：US11446881
申请日：2006-06-05
申请人： Paul H. Weigel , Kshama Kumari , Paul DeAngelis
发明人： Paul H. Weigel , Kshama Kumari , Paul DeAngelis
IPC分类号： C12N9/10 , C12P19/18
CPC分类号： C12P19/26 , A61K39/102 , C07H21/04 , C08B37/0072 , C12N9/1051 , C12P19/04 , Y10S435/836 , Y10S435/839 , Y10S435/848
摘要： The present invention relates to a recombinantly produced, enzymatically active hyaluronan synthase, wherein the recombinantly produced, enzymatically active hyaluronan synthase is a single protein that is a dual-action catalyst that utilizes UDP-GlcA and UDP-GlcNAc to synthesize HA. The recombinantly produced, enzymatically active hyaluronan synthase may be produced from a hyaluronan synthase gene isolated from a microbial source, such as a streptococcal source.

4. 发明授权

US07141409B2 Hyaluronan synthase gene and uses thereof 失效
标题翻译：透明质酸合酶基因及其用途
公开(公告)号：US07141409B2
公开(公告)日：2006-11-28
申请号：US11446871
申请日：2006-06-05
申请人： Paul H. Weigel , Kshama Kumari , Paul DeAngelis
发明人： Paul H. Weigel , Kshama Kumari , Paul DeAngelis
IPC分类号： C12N1/21 , C12N1/19 , C12N5/10 , C12P19/18 , C12N9/10
CPC分类号： C12P19/26 , A61K39/102 , C07H21/04 , C08B37/0072 , C12N9/1051 , C12P19/04 , Y10S435/836 , Y10S435/839 , Y10S435/848
摘要： The present invention relates to a recombinant host cell that includes an expression construct having a hyaluronan synthase gene that encodes an enzymatically active hyaluronan synthase (HAS), and to the use of these recombinant cells that produce hyaluronan synthase and its hyaluronic acid product.
摘要翻译：本发明涉及重组宿主细胞，其包含具有编码酶活性透明质酸合酶（HAS）的透明质酸合成酶基因的表达构建体，以及使用这些产生透明质酸合酶及其透明质酸产物的重组细胞。

5. 发明申请

US20060216793A1 HYALURONAN SYNTHASE GENE AND USES THEREOF 失效
标题翻译： HYALURONAN SYNTHASE基因及其用途
公开(公告)号：US20060216793A1
公开(公告)日：2006-09-28
申请号：US11446854
申请日：2006-06-05
申请人： Paul Weigel , Kshama Kumari , Paul DeAngelis
发明人： Paul Weigel , Kshama Kumari , Paul DeAngelis
IPC分类号： C12P19/28 , C07H21/04 , C08B37/00 , C12N15/74 , C12N1/21
CPC分类号： C12P19/26 , A61K39/102 , C07H21/04 , C08B37/0072 , C12N9/1051 , C12P19/04 , Y10S435/836 , Y10S435/839 , Y10S435/848
摘要： The present invention relates to a method of producing hyaluronic acid that includes introducing a purified nucleic acid segment having a coding region encoding enzymatically active hyaluronan synthase into a Bacillus strain, and culturing the Bacillus strain under conditions appropriate for the production of hyaluronic acid.
摘要翻译：本发明涉及一种生产透明质酸的方法，其包括将具有编码酶活性透明质酸合酶编码区的纯化核酸片段导入芽孢杆菌属菌株，并在适于生产透明质酸的条件下培养芽孢杆菌菌株。

6. 再颁专利

USRE39247E1 Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases 有权
标题翻译：耐草甘膦5-烯醇丙酮酸莽草酸-3-磷酸合成酶
公开(公告)号：USRE39247E1
公开(公告)日：2006-08-22
申请号：US10622201
申请日：2003-07-18
申请人： Gerard F. Barry , Ganesh M. Kishore , Stephen R. Padgette , William C. Stallings
发明人： Gerard F. Barry , Ganesh M. Kishore , Stephen R. Padgette , William C. Stallings
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82
CPC分类号： C12N9/1092 , C12N15/8275 , Y10S435/816 , Y10S435/822 , Y10S435/839 , Y10S435/874 , Y10S435/883 , Y10T436/143333
摘要： Genes encoding Class II EPSPS enzymes are disclosed. The genes are useful in producing transformed bacteria and plants which are tolerant to glyphosate herbicide. Class II EPSPS genes share little homology with known, Class I EPSPS genes, and do not hybridize to probes from Class I EPSPS's. The Class II EPSPS enzymes are characterized by being more kinetically efficient than Class I EPSPS's in the presence of glyphosate. Plants transformed with Class II EPSPS genes are also disclosed as well as a method for selectively controlling weeds in a planted transgenic crop field.
摘要翻译：公开了编码II类EPSPS酶的基因。这些基因可用于产生耐草甘膦除草剂的转化细菌和植物。 II类EPSPS基因与已知的I类EPSPS基因几乎没有同源性，并且不与I类EPSPS的探针杂交。 II类EPSPS酶的特征在于在草甘膦存在下比I类EPSPS更具动力学效力。还公开了用II类EPSPS基因转化的植物以及在种植的转基因作物田中选择性控制杂草的方法。

7. 发明申请

US20060013829A1 Hyaluronan synthase gene and uses thereof 失效
公开(公告)号：US20060013829A1
公开(公告)日：2006-01-19
申请号：US11226480
申请日：2005-09-14
申请人： Paul Weigel , Kshama Kumari , Paul DeAngelis
发明人： Paul Weigel , Kshama Kumari , Paul DeAngelis
IPC分类号： C07H21/04 , C08B37/00 , C12P21/06 , C12P19/28 , C12N9/24 , A61K39/02
CPC分类号： C12P19/26 , A61K39/102 , C07H21/04 , C08B37/0072 , C12N9/1051 , C12P19/04 , Y10S435/836 , Y10S435/839 , Y10S435/848
摘要： The present invention relates to a nucleic acid segment having a coding region segment encoding enzymatically active Streptococcus equisimilis hyaluronate synthase (seHAS), and to the use of this nucleic acid segment in the preparation of recombinant cells which produce hyaluronate synthase and its hyaluronic acid product. Hyaluronate is also known as hyaluronic acid or hyaluronan.

8. 发明申请

US20050271629A1 Means for biologically controlling cryptogamic plant diseases 失效
标题翻译：用于生物控制密码植物病害的手段
公开(公告)号：US20050271629A1
公开(公告)日：2005-12-08
申请号：US10520294
申请日：2003-07-01
申请人： Bernard Paul
发明人： Bernard Paul
IPC分类号： A01N63/04 , A01N63/00
CPC分类号： A01N63/04 , Y10S435/837 , Y10S435/838 , Y10S435/839 , Y10S435/874 , Y10S435/88 , Y10S435/938 , Y10S435/945 , A01N63/00 , A01N2300/00
摘要： The invention concerns the application of compositions of micro-organisms in biological control of vin crptogamic diseases. Said composition comprises a mixture of at least one bacterium and at least one yeast, the bacterium or bacteria and the yeast(s) being non-toxic for the plant. The invention also concerns bacterial and yeast strains, as well as biofungicide formulations containing an efficient amount of at least one composition of micro-organisms including in mixture at least one bacterium and one yeast, the bacterium or bacteria and the yeast(s) being non-toxic for the plant, and a composition of filamentous fungi, in particular of the genus Pichia, Pythium, Trichoderma, Gliocladium, Ampelomyces, Talaromyces, Epicococcum, combined with an inert carrier. The invention is useful for treating cryptogamic plant diseases, in particular crop plants and vine.
摘要翻译：本发明涉及微生物组合物在葡萄球菌病的生物学控制中的应用。所述组合物包含至少一种细菌和至少一种酵母，所述细菌或细菌和对植物无毒的酵母的混合物。本发明还涉及细菌和酵母菌株，以及含有有效量的至少一种微生物组合物的生物杀真菌剂，包括至少一种细菌和一种酵母混合物，细菌或细菌和酵母不是对于植物是有毒的，以及丝状真菌的组合物，特别是毕赤酵母属，腐霉属，木霉属，胶霉属，Ampelomyces，Talaromyces，Epicococcum，与惰性载体结合的组合物。本发明可用于治疗隐窝性植物病害，特别是作物植物和葡萄藤。

9. 发明授权

US06638910B2 Compositions and methods for controlling plant pests 有权
标题翻译：防治植物害虫的组成和方法
公开(公告)号：US06638910B2
公开(公告)日：2003-10-28
申请号：US09994234
申请日：2001-11-27
申请人： Sherry Darlene Heins , Denise Carol Manker , Desmond Rito Jimenez , Randy Jay McCoy , Pamela Gail Marrone , Jimmy Ensio Orjala
发明人： Sherry Darlene Heins , Denise Carol Manker , Desmond Rito Jimenez , Randy Jay McCoy , Pamela Gail Marrone , Jimmy Ensio Orjala
IPC分类号： A61K3800
CPC分类号： C07K7/06 , A01N63/00 , A01N63/02 , A61K38/00 , C12R1/125 , Y10S435/839 , A01N43/54 , A01N2300/00
摘要： The present invention relates to a novel antibiotic-producing and metabolite-producing Bacillus subtilis strain that exhibits insecticidal, antifungal and antibacterial activity. The supernatant of this novel strain contains effective insecticidal, antifungal and antibacterial agents. Also included in the invention is a solvent extractable, small molecular weight (
摘要翻译：本发明涉及具有杀虫，抗真菌和抗菌活性的新型抗生素产生和代谢产物枯草芽孢杆菌菌株。这种新菌株的上清液含有有效的杀虫，抗真菌和抗菌剂。本发明还包括在上清液中产生的溶剂可提取的小分子量（<10,000道尔顿）玉米根虫活性代谢产物。本发明还包括保护或处理植物免受真菌和细菌感染和玉米根虫侵袭的方法，包括向植物施用有效量的产生抗生素/代谢物的新型枯草芽孢杆菌菌株，由抗生素/代谢物产生的抗生素/代谢物所述新型枯草芽孢杆菌菌株或其组合，任选地还包含另一种产生抗生素的细菌菌株和/或化学农药。本发明还包括使用从新型枯草芽孢杆菌染色体的培养物中单独或与化学农药和/或其他生物防治剂组合获得的全部肉汤培养物或上清液来预防或治疗真菌和细菌感染的方法。本发明还包括称为阿斯特他汀的新型抗真菌和抗菌化合物和包含A型神经蛋白，普伐他汀，表面活性素和阿格他汀的新型组合。治疗或保护植物免受真菌和细菌感染以及玉米根虫侵袭的方法，其中包括施用新型的阿斯格他汀，并且提供包含A型神经蛋白，普伐他汀，表面活性素和非格司特汀的新型组合。还提供了从具有杀虫活性的菌株AQ713分离的脂肽提取物和从具有杀虫活性的菌株AQ713分离的表面活性素脂肽。

10. 发明申请

US20020106784A1 METHOD AND APPARATUS FOR THE BIOLOGICAL CONVERSION OF ORGANIC WASTE INTO HUMIC PRODUCTS 失效
标题翻译：有机废物生物转化为人造产品的方法与装置
公开(公告)号：US20020106784A1
公开(公告)日：2002-08-08
申请号：US09257011
申请日：1999-02-25
发明人： RICCARDO REVERSO
IPC分类号： C07F001/00 , C05F011/08 , C12M001/00
CPC分类号： C05F17/0027 , C05F17/0036 , Y02P20/145 , Y02W30/43 , Y10S435/822 , Y10S435/831 , Y10S435/832 , Y10S435/834 , Y10S435/838 , Y10S435/839 , Y10S435/853 , Y10S435/854 , Y10S435/855 , Y10S435/856 , Y10S435/857 , Y10S435/878 , Y10S435/886 , Y10S435/898 , Y10S435/904 , Y10S435/911 , Y10S435/921 , Y10S435/942
摘要： A method for converting organic solid waste into humic products and the corresponding apparatus, in which a preliminary aerobic fermentation of the organic solid waste is performed, followed by a second aerobic fermentation of the organic waste in the presence of specific microorganisms in order to provide a basic precursor on which the selective metabolization occurs of specific bacterial strains to provide the humic product.
摘要翻译：一种将有机固体废物转化成腐殖质的方法，以及相应的装置，其中进行有机固体废物的初步好氧发酵，然后在特定微生物存在下对有机废物进行第二次好氧发酵，以提供选择性代谢发生特定细菌菌株以提供腐殖质的基本前体。

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