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1. 发明申请

US20230136050A1 METHOD OF CLEANING LIQUID CHROMATOGRAPHIC SYSTEM AND LIQUID CHROMATOGRAPHIC SYSTEM 有权
公开(公告)号：US20230136050A1
公开(公告)日：2023-05-04
申请号：US17971605
申请日：2022-10-23
申请人： SHIMADZU CORPORATION
发明人： Keisuke OGAWA , Andy SASAKI , Kerry HILL , Timothy LEE
IPC分类号： G01N30/46 , G01N30/32 , G01N30/20 , B01D15/18 , B01D15/16 , B01D15/14 , B01D15/42 , B08B9/032
摘要： A liquid chromatographic system includes a column, a needle, a valve, a pump, and an analysis flow path. The valve sets the analysis flow path by switching between a first flow path through which an eluent flows from the valve to the column via the needle and a second flow path through which the eluent flows from the valve to the column not via the needle. The first flow path can be cleaned by drive of a first cleaning pump while the first flow path is set, and the second flow path can be cleaned by drive of the first cleaning pump while the second flow path is set.

2. 发明申请

US20230134182A1 METHOD FOR EFFICIENTLY SEPARATING AND PURIFYING RECOMBINANT HUMAN COAGULATE FACTOR VIII FC FUSION PROTEIN 有权
公开(公告)号：US20230134182A1
公开(公告)日：2023-05-04
申请号：US17907724
申请日：2021-03-16
申请人： AMPSOURCE BIOPHARMA SHANGHAI INC.
发明人： Shixiang Jia , Shidong Xu , Lingju Yu , Bo Zhou , Hong Wu , Yonglong Yuan , Qiang Li
IPC分类号： C07K1/36 , C07K14/755 , B01D15/38 , B01D15/36 , B01D15/42 , B01D15/20 , B01D15/32
摘要： Disclosed is a method for efficiently separating and purifying recombinant human coagulate factor VIII Fc fusion protein. The method comprises steps of affinity chromatography and anion exchange chromatography; and the sample captured by means of the affinity chromatography is eluted with a salt ion buffer containing 5%-20% polyol organic solvents under the condition of pH 4.0 to 8.0, and the protein sample can be separated and purified to 85% or more by further ProteinA affinity chromatography. The purification method is simple to operate, naturally connects each step of chromatography, has a high recovery rate and low cost, and easily increases production.

3. 发明授权

US11639515B2 Genetically engineered strain for producing porcine myoglobin and food-grade fermentation and purification thereof 有权
公开(公告)号：US11639515B2
公开(公告)日：2023-05-02
申请号：US17743716
申请日：2022-05-13
申请人： TAIXING DONGSHENG BIO-TECH CO., LTD , Jiangnan University
发明人： Xinrui Zhao , Bohan Zhang , Jingwen Zhou , Guocheng Du , Jianghua Li , Jian Chen , Fei Yu , Wei Lu , Yuan Qian
IPC分类号： C07K14/805 , A23L27/26 , C12N1/16 , A23J1/18 , C12N15/81 , A23J3/04 , C12P21/02 , C12N15/52 , B01D15/42 , B01D15/36 , B01D15/34 , B01D69/02 , C12R1/84
摘要： The disclosure discloses a genetically engineered strain for producing porcine myoglobin and fermentation and purification thereof, and belongs to the technical field of genetic engineering. The disclosure realizes efficient secretion and expression of porcine myoglobin by integrating the gene of porcine myoglobin in P. pastoris. On this basis, optimization of the medium and culture conditions of recombinant P. pastoris can significantly increase the titer of porcine myoglobin, so that the titer can reach 285.42 mg/L under fermenter conditions. In addition, by creatively adding different concentrations of ammonium sulfate to fermentation broth step by step, the purity of myoglobin obtained by final concentration is up to 88.0%, and the purification rate is up to 66.1%. The disclosure realizes efficient expression and high purification of porcine myoglobin from various steps such as synthesis, fermentation and purification of porcine myoglobin, and provides broad prospects for industrial production of porcine myoglobin.

4. 发明申请

US20230094669A1 METHOD FOR SEPARATING CARBON NANOTUBES USING MODIFIED CELLLOSE 有权
公开(公告)号：US20230094669A1
公开(公告)日：2023-03-30
申请号：US17905248
申请日：2021-03-11
申请人： YAZAKI CORPORATION
发明人： Ayrat Maratovich Dimiev , Timur Lenarovich Khamidullin
IPC分类号： C01B32/172 , C01B32/174 , C01B32/159 , B01J20/24 , B01J20/28 , B01J20/288 , B01D15/42
摘要： The present invention relates to using modified cellulose (e.g., nitrated cellulose) for separating carbon nanotubes (CNTs). A raw mixture of CNTs of different structures or chiral angles (chiralities), can be separated into fractions, based on their selective permeation through a separation column filled with nitrated cellulose. The present invention is particularly useful in separating semiconducting CNTs and metallic CNTs.

5. 发明申请

US20230001327A1 AUTOMATIC SEPARATION APPARATUS FOR FOUR FRACTIONS OF HEAVY OIL AND SEPARATION METHOD THEREOF 有权
公开(公告)号：US20230001327A1
公开(公告)日：2023-01-05
申请号：US17772367
申请日：2020-10-26
申请人： PETROCHINA COMPANY LIMITED , BEIJING ORIENDA INSTRUMENTS CO., LTD.
发明人： Qing Cao , Dawei Wen , Shengbao He , Yuan Xiu , Lin Tian , Dingzhong Yang , Jing He , Minna Dou , Fei Chen , Kunhong Liu , Chenchen Li , Huifeng Xue , Jianming Wang
IPC分类号： B01D15/18 , B01D15/12 , B01D15/42 , B01D63/08 , B01D69/02 , C10G25/00 , B01J20/08 , B01J20/28 , G01N30/06 , G01N30/38
摘要： The present disclosure provides an automatic separation apparatus for four fractions of heavy oil and a separation method thereof, wherein the apparatus includes a solvent reservoir tank (1), a separation unit for four fractions of heavy oil (100) and a receiving apparatus (9). The separation unit for four fractions of heavy oil (100) includes: a filter disc (4) having one end in communication with the solvent reservoir tank (1), and the other end in communication with an inlet of a pre-column flow path switching valve (5); a chromatographic column (6) having an inlet in communication with an outlet of the pre-column flow path switching valve (5), and an outlet in communication with an inlet of a post-column flow path switching valve (8). The receiving apparatus is in communication with an outlet of the post-column flow path switching valve (8).

6. 发明授权

US11478524B1 Methods and compositions for modulating complex mixtures by selective deletion chromatography 有权
公开(公告)号：US11478524B1
公开(公告)日：2022-10-25
申请号：US16050621
申请日：2018-07-31
申请人： UNIVERSITY OF SOUTH FLORIDA
发明人： Bill J. Baker
IPC分类号： B01D15/16 , B01D15/24 , B01D15/32 , B01D15/42 , G01N30/34 , G01N30/72 , G01N30/74 , G01N30/82 , G01N30/86 , G01N30/02 , G01N30/84 , A61K36/82
摘要： The present disclosure relates to methods of modifying complex extracts such that components or mixtures of components are selectively removed or added, thus providing a complex mixture that does not naturally occur with a refined or a tuned therapeutic or nutraceutical effect. In various aspects, the complex extract can be an extract obtained from one or more plants, e.g., an extract obtained from green tea leaves. The present disclosure pertains to compositions obtained by the disclosed methods, nutraceutical compositions comprising same, pharmaceutical compositions comprising same, and methods of treating various conditions, including physiological dysfunctions associated with elevated reactive oxygen species and/or inflammatory molecule, e.g., TNFα, expression using same. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

7. 发明申请

US20220290212A1 RAPID ISOLATION AND COLLECTION OF MICROBIAL RNA FROM A BIOLOGICAL SPECIMEN 有权
公开(公告)号：US20220290212A1
公开(公告)日：2022-09-15
申请号：US17638111
申请日：2020-08-28
申请人： Contamination Source Identification LLC
发明人： Regina Lamendella , Justin Wright , Alexander Shope
IPC分类号： C12Q1/689 , B01D15/16 , B01D15/24 , B01D15/42 , B01D15/34 , C12Q1/6806
摘要： Detection and identification of microbial species uses liquid chromatography as a bulk filtration process for rapid isolation and collection of microbial RNA from a biological specimen. In various embodiments, gene sequencing of microbial RNA molecules from a biological specimen is enhanced by obtaining and then preparing the biological specimen as a test sample for liquid chromatography that is used to bulk filter microbial RNA molecules from a mixture of RNA molecules in the test sample to isolate and collect the microbial RNA molecules in two or more fraction outputs, wherein at least one of the two or more fraction outputs is a fraction within a void volume of the liquid chromatography, preparing one or more fraction outputs for gene sequencing, including the fraction output that is within the void volume, and conducting gene sequencing on the one or more prepared outputs to detect microbial RNA from the biological specimen.

8. 发明授权

US11428696B2 Mass spectrometry analysis of mutant polypeptides in biological samples 有权
公开(公告)号：US11428696B2
公开(公告)日：2022-08-30
申请号：US16890379
申请日：2020-06-02
申请人： Cell Signaling Technology, Inc.
发明人： Albrecht Moritz , John Edward Rush, II , Roberto Polakiewicz
IPC分类号： G01N33/68 , B01D15/12 , B01D15/32 , B01D15/38 , B01D15/42 , G01N30/72 , H01J49/00
摘要： The invention relates to a method for determining the presence of at least one distinct polypeptide in a biological sample comprising contacting the biological sample with a hydrolyzing agent, wherein the hydrolyzing agent is capable of hydrolyzing the distinct polypeptide in a sequence-specific manner such that at least one distinct peptide having a predetermined peptide measured accurate mass would result if the at least one distinct polypeptide were present in the biological sample, to obtain a hydrolyzed sample; bringing the hydrolyzed sample in contact with a substrate comprising at least one immobilized binding partner, wherein the at least one immobilized binding partner is capable of specifically binding the distinct peptide; removing the hydrolyzed sample from the substrate in a manner such that the distinct peptide would remain bound to the immobilized binding partner; contacting the substrate with an elution solution, wherein the distinct peptide would dissociate from the immobilized binding partner into the elution solution; subjecting a portion of the elution solution to liquid chromatography to segregate a plurality of molecules in the portion of the elution solution to obtain sorted molecules; determining the measured accurate mass of at least one sorted molecule present in the elution solution; and determining the presence of the at least one distinct polypeptide in the biological sample when a measured accurate mass of at least one molecule is substantially equal to the predetermined peptide measured accurate mass.

9. 发明申请

US20220267823A1 Genetically Engineered Strain for Producing Porcine Myoglobin and Food-grade Fermentation and Purification Thereof 有权
公开(公告)号：US20220267823A1
公开(公告)日：2022-08-25
申请号：US17743716
申请日：2022-05-13
申请人： JIANGSU DONGHUI BIO-TECH CO., LTD , Jiangnan University
发明人： Xinrui Zhao , Bohan Zhang , Jingwen Zhou , Guocheng Du , Jianghua Li , Jian Chen , Fei Yu , Wei Lu , Yuan Qian
IPC分类号： C12P21/02 , C12N1/16 , C12N15/52 , C12N15/81 , C07K14/805 , A23J1/18 , B01D15/42 , B01D15/36 , B01D15/34 , B01D69/02
摘要： The disclosure discloses a genetically engineered strain for producing porcine myoglobin and fermentation and purification thereof, and belongs to the technical field of genetic engineering. The disclosure realizes efficient secretion and expression of porcine myoglobin by integrating the gene of porcine myoglobin in P. pastoris. On this basis, optimization of the medium and culture conditions of recombinant P. pastoris can significantly increase the titer of porcine myoglobin, so that the titer can reach 285.42 mg/L under fermenter conditions. In addition, by creatively adding different concentrations of ammonium sulfate to fermentation broth step by step, the purity of myoglobin obtained by final concentration is up to 88.0%, and the purification rate is up to 66.1%. The disclosure realizes efficient expression and high purification of porcine myoglobin from various steps such as synthesis, fermentation and purification of porcine myoglobin, and provides broad prospects for industrial production of porcine myoglobin.

10. 发明申请

US20220259119A1 COMPOUNDS FOR CHROMATOGRAPHIC SEPARATION OF RARE EARTH ELEMENTS AND S-, P-, D-METALS, METHOD OF SEPARATION, AND USE THEREOF 有权
公开(公告)号：US20220259119A1
公开(公告)日：2022-08-18
申请号：US17616340
申请日：2020-05-28
申请人： USTAV ORGANICKE CHEMIE A BIOCHEMIE AV CR, V.V.I
发明人： Miloslav Polasek
IPC分类号： C07B59/00 , C07D401/06 , C07F9/6524 , C07D401/14 , B01D15/42
摘要： The present invention relates to compounds of general formula (I), wherein —X is selected from a group consisting of H; C1 to C6, alkyl; halogen (F, Cl, Br or I); Y is selected from a group consisting of nitrogen; N-oxide; Z1, Z2, Zm, wherein m is 1 or 2, are independently selected from the group consisting of —CH2—CH2— and —CH2—CH2—CH24 A, Am, wherein m is 1 or 2, are independently selected from H; —CH2COOH; —CH2C(O)NH2; —CH2P(O)(OH)2, and n is 1 or 2; R1, R2, R3 are independently H; C1 to C6, alkyl; C1 to C6 alkyloxy; C6 to C10 aryloxy; benzyloxy; C1 to C6 alkylthio; C6, to C10 arylthio; F; Cl; Br; I; OH; SH; NH2; C1 to C6, alkylamino; di(C1 to C6, alkyl)amino; C1 to C6 acylamino; di(C1 to C6 acyl) amino; C6 to C10 arylamino; di(C6 to C10 aryl)amino; CN; OH; nitro; COORn, C(O)NHRn, C(O)N(Rn)2, wherein Rn is independently H or C1 to C10 alkyl or C6, to C10 aryl; and/or neighboring two of R1, R2, R3 together with neighboring two carbon atoms of the aromatic cycle form a six-membered ring, optionally substituted with one or more substituents independently selected from the group consisting of OH, SH, CF3, F, Cl, Br, I, C1 to C6, alkyl, C1to C6, alkyloxy, C1 to C6 alkylthio, NH2, C1 to C6 alkylamino, di(C1 to C6 alkyl)amino, NO2, COOH, COORn, C(O)NHRn, C(O)N(Rn)2, wherein Rn is WO 2020/244686 A1 independently H or C1 to C10 alkyl or C6 to C10 aryl; with the proviso that when n is 2 and all of Z1, Z2, Zm are —CH2—CH2—, then A is not —CH2COOH; for chromatographic separation of rare earth elements and/or s-, p- and d-block metals, as well as to the method of the separation of rare earth elements.

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