专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06589772B1 Acidophile archaeal organism 失效
标题翻译：嗜酸性古细菌
公开(公告)号：US06589772B1
公开(公告)日：2003-07-08
申请号：US09515996
申请日：2000-02-29
申请人： Jillian F. Banfield , Katrina J. Edwards , Philip L. Bond , Thomas M. Gihring
发明人： Jillian F. Banfield , Katrina J. Edwards , Philip L. Bond , Thomas M. Gihring
IPC分类号： C12N100
CPC分类号： C12R1/00
摘要： A new species of acidophilic microorganism has bee isolated from a acid mine drainage site. The organism is an archaea here given the tentative species designation Ferroplasma acidarmanus and the strain designation fer1. This organism is tolerant of extraordinary conditions of low pH and high metal concentrations.
摘要翻译：一种新的嗜酸性微生物从酸性矿井排水点分离出蜜蜂。这里的生物体是一种古菌，这里给出了试剂种名称Ferroplasma acidarmanus和菌株名称fer1。该生物体耐受低pH和高金属浓度的非凡条件。

2. 发明授权

US06558940B2 Streptomyces strain with potential anti-microbial activity against phytopathogenic fungi 失效
标题翻译：链霉菌菌株对植物病原真菌具有潜在的抗微生物活性
公开(公告)号：US06558940B2
公开(公告)日：2003-05-06
申请号：US09538456
申请日：2000-03-30
申请人： Mansoor Alam , Abdul Sattar , Sushil Kumar , Abdul Samad , Om Prakash Dhawan , Suman Preet Singh Khanuja , Ajit Kumar Shasany , Seema Singh , Poovappallivadakethil Viswanathan Nair Ajaya Kumar , Abdul Khaliq , Mohammad Zaim , Saba Shahabuddin , Mala Trivedi
发明人： Mansoor Alam , Abdul Sattar , Sushil Kumar , Abdul Samad , Om Prakash Dhawan , Suman Preet Singh Khanuja , Ajit Kumar Shasany , Seema Singh , Poovappallivadakethil Viswanathan Nair Ajaya Kumar , Abdul Khaliq , Mohammad Zaim , Saba Shahabuddin , Mala Trivedi
IPC分类号： C12N100
CPC分类号： C12R1/465 , C12N1/20
摘要： The present invention relates to a new strain of Streptomyces sp., called CIMAP A1 isolated from the soil of geranium (Pelargonium graveolens) planted in the experimental fields of CIMAP and having the accession No. ATCC PTA-4131 and capable of inhibiting the growth of phytopathogenic fungi.
摘要翻译：本发明涉及从CIMAP的实验领域种植的具有登录号ATCC PTA-4131并能够抑制生长的天竺葵（Pelargonium graveolens）土壤中分离出的称为CIMAP A1的新型链霉菌属菌株植物病原真菌

3. 发明授权

US06482635B2 Method of reducing off-flavor in water or aquatic life forms using Bacterium NRRL B-30043 失效
标题翻译：使用细菌NRRL B-30043减少水中的异味或水生生物形式的方法
公开(公告)号：US06482635B2
公开(公告)日：2002-11-19
申请号：US09967376
申请日：2001-09-28
申请人： Harrell L. Walker , Lawrence R. Higginbotham
发明人： Harrell L. Walker , Lawrence R. Higginbotham
IPC分类号： C12N100
CPC分类号： C12R1/01 , A01N63/00 , C12N15/11 , Y10S435/822 , Y10S435/91
摘要： A method is provided for reducing or eliminating off-flavor in water or fish by controlling cyanobacteria or algae in the water or fish wherein the cyanobacteria or algae produce agents that cause the off-flavor. The cyanobacteria or algae are susceptible to a new Bacterium NRRL B-30043 which heretofore has not been identified or recognized as a useful agent in controlling cyanobacteria or algae. By simply treating a body of water having an off-flavor with Bacterium NRRL B-30043, the off-flavor is reduced or eliminated. Commercial fisheries or nurseries which produce channel catfish for human consumption will benefit from using this environmentally friendly Bacterium NRRL B-30043 to reduce or eliminate off-flavor in the catfish.
摘要翻译：提供了一种通过控制水或鱼中的蓝细菌或藻类来减少或消除水或鱼中的异味的方法，其中蓝细菌或藻类产生导致异味的试剂。蓝细菌或藻类对新的细菌NRRL B-30043是敏感的，迄今尚未被鉴定或被认为是控制蓝细菌或藻类的有用试剂。通过使用细菌NRRL B-30043简单地处理具有异味的身体，减少或消除异味。生产鲶鱼用于人类消费的商业渔业或苗圃将受益于使用这种环保的细菌NRRL B-30043来减少或消除鲶鱼中的异味。

4. 发明授权

US06461853B1 Method for surface culture of microorganisms and cells in flexible culture bags 失效
标题翻译：柔性培养袋中微生物和细胞表面培养的方法
公开(公告)号：US06461853B1
公开(公告)日：2002-10-08
申请号：US09858451
申请日：2001-05-17
申请人： Hong Zhu
发明人： Hong Zhu
IPC分类号： C12N100
CPC分类号： C12M23/14 , C12M23/46 , C12M23/48 , C12N1/00
摘要： A method of using flexible bags for surface culture of microorganisms and cells on a stationary interface between a gaseous phase and a liquid medium and the devices upon which the method are practiced are disclosed. A flexible culture bag is first filled with a liquid growth medium, a sample inoculum, and a predetermined amount of air to create a headspace. The bag is then sealed and placed on a stationary platform, and mounted with an external self-support device on the top web of the bag. The external support device holds the top web of the bag in a fixed position and, thus, prevents the bag from deflating so that a stationary interface between the headspace and the liquid medium in the bag is maintained throughout the cultivation period.
摘要翻译：公开了一种在气相和液体介质之间的固定界面上使用柔性袋用于表面培养微生物和细胞的方法以及实施该方法的装置。首先将柔性培养袋填充液体生长培养基，样品接种物和预定量的空气以产生顶部空间。然后将袋密封并放置在固定的平台上，并在袋的顶部网上安装有外部自支撑装置。外部支撑装置将袋的顶部网保持在固定位置，并且因此防止袋放气，使得整个培养期间保持袋中的顶部空间和液体介质之间的固定界面。

5. 发明授权

US06436694B1 Regulable gene expression in gram-positive bacteria 失效
标题翻译：革兰氏阳性菌中可调节的基因表达
公开(公告)号：US06436694B1
公开(公告)日：2002-08-20
申请号：US09291874
申请日：1999-04-14
申请人： Francis P. Tally , Jianshi Tao , Xiaoyu Shen , Jiansu Zhang
发明人： Francis P. Tally , Jianshi Tao , Xiaoyu Shen , Jiansu Zhang
IPC分类号： C12N100
CPC分类号： C12N15/8509 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2217/20 , A01K2267/0337 , A01K2267/0393 , C07K2319/00 , C12N15/635 , C12N15/74 , C12Q1/025 , G01N33/5008 , G01N33/502 , G01N33/5088 , G01N2333/31
摘要： A system has been constructed which is suitable for tetracycline-inducible gene expression in Gram-positive bacteria such as Staphylococcus aureus and Bacillus subtilis. The replicon/host gene expression system is tightly regulated, can be used in complex as well as minimal media, and can produce a high level of gene expression upon induction, with a variety of gene products. The gene expression system is suitable for production of products toxic to the host cells, and can be used, for example, for the analysis of gene expression and gene products in Gram-positive bacteria, and in a test of the effect of a peptide or polypeptide inhibitor of an S. aureus enzyme on the growth of S. aureus cells in culture or during infection of an animal.
摘要翻译：已经构建了适用于革兰氏阳性菌如金黄色葡萄球菌和枯草芽孢杆菌中四环素诱导型基因表达的系统。复制子/宿主基因表达系统受到严格调控，可用于复合物和基本培养基，并可在诱导时产生高水平的基因表达，并具有多种基因产物。该基因表达系统适用于生产对宿主细胞有毒的产品，可用于例如革兰氏阳性菌中基因表达和基因产物的分析，以及对肽的作用或金黄色葡萄球菌酶的多肽抑制剂对培养物或感染动物期间的金黄色葡萄球菌细胞的生长。

6. 发明授权

US06262339B1 Process for generating male sterile plants 失效
标题翻译：产生雄性不育植物的方法
公开(公告)号：US06262339B1
公开(公告)日：2001-07-17
申请号：US08556944
申请日：1996-04-02
申请人： Rob Dirks , Klaus Trinks , Bert Uijtewaal , Klaus Bartsch , Roger Peeters , Rainer Höfgen , Hans-Dieter Pohlenz
发明人： Rob Dirks , Klaus Trinks , Bert Uijtewaal , Klaus Bartsch , Roger Peeters , Rainer Höfgen , Hans-Dieter Pohlenz
IPC分类号： C12N100
CPC分类号： C12N9/88 , C12N15/8289
摘要： The invention is directed to a process for the generation of male sterility in plants comprising the steps of (a) transforming a plant cell with DNA sequences that selectively inhibit the expression of essential metabolic compounds and (b) regenerating plants from said plant cells. Cells impaired in the biosynthesis of basic metabolic compounds undergo starvation and eventually die. Such pathways include amino acid biosynthesis, nucleic acid biosynthesis and other biosynthetic pathways such as citric acid cycle, pentose phosphate pathway, fatty acid metabolism, vitamin biosynthesis that will render the cell inactive due to nutrient depletion, if one or more enzymes or proteins involved in this pathway would become inactive by using inhibitory DNA constructs.
摘要翻译：本发明涉及在植物中产生雄性不育的方法，包括以下步骤：（a）用选择性抑制必需代谢化合物表达的DNA序列转化植物细胞，和（b）从所述植物细胞再生植物。碱性代谢化合物生物合成中受损的细胞经历饥饿并最终死亡。这样的途径包括氨基酸生物合成，核酸生物合成和其他生物合成途径，例如柠檬酸循环，戊糖磷酸途径，脂肪酸代谢，维生素合成，如果参与的一种或多种酶或蛋白质会使细胞由于营养物质消耗而失活通过使用抑制性DNA构建体，该途径将变得无活性。

7. 发明授权

US06258569B1 Hybridization assay using self-quenching fluorescence probe 有权
标题翻译：使用自熄荧光探针的杂交测定
公开(公告)号：US06258569B1
公开(公告)日：2001-07-10
申请号：US09436454
申请日：1999-11-08
申请人： Kenneth J. Livak , Susan J. A. Flood , Jeffrey Mamoro , Khairuzzaman Bashar Mullah
发明人： Kenneth J. Livak , Susan J. A. Flood , Jeffrey Mamoro , Khairuzzaman Bashar Mullah
IPC分类号： C12N100
CPC分类号： C12Q1/6818 , C12Q1/6834 , C12Q1/6837 , C12Q2565/519 , C12Q2563/107 , C12Q2561/101 , C12Q2525/185 , C12Q2525/301 , C12Q2565/1015 , C12Q2565/107
摘要： Provided is a method of nucleic acid amplification. In one embodiment, the method comprises performing nucleic acid amplification on a target polynucleotide using a nucleic acid polymerase having 5′ to 3′ nuclease activity, a primer capable of hybridizing to the target polynucleotide, and an oligonucleotide probe under amplification conditions such that the probe hybridizes to the target polynucleotide 3′ relative to the primer and the probe does not hybridize with itself to form a hairpin structure. The oligonucleotide probe has at one end a fluorescent reporter and at the other end a quencher that quenches the fluorescence of the reporter molecule when both the fluorescent reporter and quencher are attached to the probe. Digestion of the oligonucleotide probe by the polymerase during amplification is effective to separate the reporter from the quencher, whereby a fluorescence signal of the reporter is increased.
摘要翻译：提供了核酸扩增的方法。在一个实施方案中，所述方法包括使用具有5'至3'核酸酶活性的核酸聚合酶在靶多核苷酸上进行核酸扩增，能够与靶多核苷酸杂交的引物和寡核苷酸探针在扩增条件下进行，使得探针与靶多核苷酸3'相对于引物杂交，并且探针不与其自身杂交以形成发夹结构。寡核苷酸探针在一端具有荧光报告基团，另一端是当荧光报道分子和猝灭剂连接到探针上时淬灭报道分子的荧光的猝灭剂。在扩增期间通过聚合酶消化寡核苷酸探针有效地将报道分子从猝灭剂中分离出来，从而增加了报道分子的荧光信号。

8. 发明授权

US06187555B1 Spores with increased sensitivity to sterilants using additives that bind to sterilant-sensitive sites 失效
标题翻译：使用与杀菌剂敏感部位结合的添加剂对灭菌剂敏感性更高的孢子
公开(公告)号：US06187555B1
公开(公告)日：2001-02-13
申请号：US09061293
申请日：1998-04-16
申请人： Kestutis J. Tautvydas
发明人： Kestutis J. Tautvydas
IPC分类号： C12N100
CPC分类号： A61L2/07 , A61L2/14 , A61L2/206 , A61L2/28 , C12N1/04 , Y10S435/822 , Y10S435/832 , Y10S435/839
摘要： Microbial spores having increased sensitivity to sterilants are provided. An additive such as a dipeptide, oligosaccharide, and/or polyhydroxyalcohols are added to the spores wherein the additive is bound to sterilant-sensitive sites in the spores. The additive increases sensitivity of the spores to a sterilant. More than one additive can be utilized to alter the sensitivity of the spores to a sterilant. Biological indicators comprising the microbial spores and a solid support are also disclosed and those spores having a dipeptide specifically bound to sterilant-sensitive sites in the spores have an altered sensitivity to a sterilant. Furthermore, a method is disclosed for altering the sensitivity of microbial spores to a sterilant comprising drying the spores at a temperature between 35° C. and 55° C. in a liquid composition having an amount of the additive therein.
摘要翻译：提供了对灭菌剂敏感性增加的微生物孢子。将添加剂如二肽，寡糖和/或多羟基醇添加到孢子中，其中添加剂与孢子中的杀菌剂敏感位点结合。添加剂增加了孢子对杀菌剂的敏感性。可以使用多于一种的添加剂来改变孢子对杀菌剂的敏感性。还公开了包含微生物孢子和固体支持物的生物指示剂，并且具有与孢子中灭菌剂敏感位点特异性结合的二肽的那些孢子具有改变的对消毒剂的敏感性。此外，公开了一种用于改变微生物孢子对灭菌剂的敏感性的方法，包括在具有一定量添加剂的液体组合物中，在35℃和55℃之间的温度下干燥孢子。

9. 发明授权

US06821768B2 Corynebacterium ammoniagenes KCCM 10340 for producing 5'-xanthylic acid 有权
标题翻译：产氨酸棒杆菌KCCM 10340用于产生5'-戊酸
公开(公告)号：US06821768B2
公开(公告)日：2004-11-23
申请号：US10322455
申请日：2002-12-19
申请人： Jeong-Hwan Kim , Young-Hyeon Kwag , Jang-Hee Park , Eun-Sung Koh , Yoon-Suk Oh , Jea-Young Chang , Kwang-Ho Lee , Jae-Ick Sim , Jong-Kwon Han , Young-Hoon Park
发明人： Jeong-Hwan Kim , Young-Hyeon Kwag , Jang-Hee Park , Eun-Sung Koh , Yoon-Suk Oh , Jea-Young Chang , Kwang-Ho Lee , Jae-Ick Sim , Jong-Kwon Han , Young-Hoon Park
IPC分类号： C12N100
CPC分类号： C12R1/15 , C12N1/20 , C12P19/32 , Y10S435/843
摘要： The invention relates to microorganism producing 5′-xanthylic acid. A 3,4-dehydro-DL-proline resistant Corynebacterium ammoniagenes CJXPK001 strain is provided for producing 5′-xanthylic acid. It is a mutant strain of Corynebacterium ammoniagenes KFCC 10743 having a lower resistance to 3,4-dehydro-DL-proline. The mutant strain is not affected by osmotic pressure caused by accumulated, highly concentrated solute in a culture medium, and it can produce 5′-xanthylic acid at a high yield and concentration rate.
摘要翻译：本发明涉及产生5'-戊酸的微生物。提供3,4-脱氢-DL-脯氨酸抗性产氨杆菌CJXPK001菌株以产生5'-戊酸。它是具有对3,4-脱氢-DL-脯氨酸的较低抗性的产氨棒杆菌KFCC 10743的突变菌株。突变株不受培养基中积累，高度浓缩的溶质引起的渗透压的影响，可以高产率和浓度产生5'-叶酸。

10. 发明授权

US06787347B2 Process for the preparation of amino alcohols and derivatives thereof and microorganisms useful in process 失效
标题翻译：用于制备氨基醇及其衍生物和可用于工艺的微生物的方法
公开(公告)号：US06787347B2
公开(公告)日：2004-09-07
申请号：US09992982
申请日：2001-11-14
申请人： Christine Bernegger-Egli , Olwen M. Birch , Pierre Bossard , Walter Brieden , Frank Brux , Knut Burgdorf , Laurent Duc , Kay-Sarah Etter , Yves Guggisberg , Martin Sauter , Eva Maria Urban
发明人： Christine Bernegger-Egli , Olwen M. Birch , Pierre Bossard , Walter Brieden , Frank Brux , Knut Burgdorf , Laurent Duc , Kay-Sarah Etter , Yves Guggisberg , Martin Sauter , Eva Maria Urban
IPC分类号： C12N100
CPC分类号： C12P13/001 , C07C215/42 , C07D209/52 , C12N9/80 , C12P13/02 , C12P41/007 , C12R1/01
摘要： The invention relates to a novel process for the preparation of (1R,4S)- or (1S,4R)-1-amino-4-(hydroxymethyl)-2-cyclopentene of the formulae and/or of (1S,4R)- or (1R,4S)-amino alcohol derivatives of the general formulae and to novel microorganisms which are able to utilize a cyclopentene derivative of the general formula as sole nitrogen source, as sole carbon source or as sole carbon and nitrogen source.
摘要翻译：本发明涉及式（1R，4S） - 或（1S，4R）-1-氨基-4-（羟甲基）-2-环戊烯的新颖方法和/或（1S，4R） - 或（1R，4S） - 氨基醇衍生物，以及能够利用通式为唯一氮源的环戊烯衍生物作为唯一碳源或唯一碳源和氮源的新型微生物。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式