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    • 95. 发明申请
    • Microfluidic Cell Sorter System
    • 微流控细胞分选系统
    • US20080213821A1
    • 2008-09-04
    • US11579074
    • 2005-05-06
    • Ai Qun LiuXiao Jun LiangXu Ming ZhangYi SunPeter Droge
    • Ai Qun LiuXiao Jun LiangXu Ming ZhangYi SunPeter Droge
    • C12M1/34C12M1/36C12Q1/20H05H1/00
    • G01N15/1484B01L3/502761B01L3/502776B01L2200/0636B01L2200/0647B01L2300/0645B01L2300/0654B01L2300/0816B01L2300/0864B01L2400/0418B01L2400/0622B81B2201/058B81C1/00444G01N15/1459G01N2015/149
    • A microfluidic system for separating, purifying and counting cell sub-populations, utilising steering of liquid flows in microfluidic channels in a cell focusing region (first dotted circle area); having the integration of the optical detection mechanism and a microchannel structure made from moulding. A master is photolithographically patterned on a soft PDMS silicon or polymer material. After being moulded and peeled off the master, the micro-channel structure is sealed on a hard substrate with openings punched through for wells (14, 12, 36, 38, 40). An optical detection region (20) discriminates different types of cells that have been formed into a single flow (30). Electromagnetic fields are used to steer (32) the flows of cells according to the signals from the optical detection region into branch channels leading to the punched wells for separate collection. The system can have parallel systems that increase throughput or cascade systems to provide several analysis steps. The optical system an micro-lens (24) for the system can be imbedded in the moulding material during formation of the mould.
    • 一种用于分离,纯化和计数细胞亚群的微流体系统,利用在细胞聚焦区域(第一虚线圆形区域)的微流体通道中的液体流的转向; 具有光学检测机构的一体化和由成型制成的微通道结构。 在软PDMS硅或聚合物材料上光刻成型的母版。 在模制和剥离主体之后,微通道结构被密封在具有穿孔的开口(14,12,36,38,40)的硬质基底上。 光学检测区域(20)区分已形成单个流(30)的不同类型的单元。 电磁场用于根据来自光学检测区域的信号将细胞流引导(32)到分支通道,通向冲孔以便分开收集。 该系统可以具有增加吞吐量或级联系统的并行系统,以提供多个分析步骤。 用于系统的微透镜(24)的光学系统可以在模具形成期间嵌入模制材料中。