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1. 发明授权

US5041387A Determination of the acid-base status of blood 失效
标题翻译：测定血液的酸碱状态
公开(公告)号：US5041387A
公开(公告)日：1991-08-20
申请号：US882638
申请日：1986-07-07
申请人： Rolf Zander , Hans U. Wolf
发明人： Rolf Zander , Hans U. Wolf
IPC分类号： G01N21/80 , G01N33/487 , G01N33/49 , G01N33/72 , G01N33/84
CPC分类号： G01N33/4925 , G01N33/84 , Y10T436/204998
摘要： In order to simplify the operation for determination of the acid-base status of blood, composed of the values for pH, pCO.sub.2 and the base excess BE, the base excess BE is determined through measurement of the pH-value at a pCO.sub.2 of about 0 mn Hg. The pH-values may be determined photometrically, in which case the indicator solution for measuring the actual blood-pH is an aqueous solution of 40 micromol/l bromthymol blue plus 0.2 g/l sodium dodecylsulfate plus 1% ethanol; and for measurement of the base excess BE is an aqueous solution of 65 micro mol/l naphtholphthalein plus 0.2 g/l sodium dodecylsulfate plus 15% dimethylsulfoxide, which are measured at a wavelength of 635 nm. If desired, one may use a single solution for measuring both actual blood-pH as well as the base excess BE, composed of 16 micromol/l bromthymol blue plus 34 micromol/l naphtholphthalein plus 0.15 g/l sodium dodecylsulfate, which is measured at 615 nm wavelength.

2. 发明授权

US4454229A Determination of the acid-base status of blood 失效
标题翻译：测定血液的酸碱状态
公开(公告)号：US4454229A
公开(公告)日：1984-06-12
申请号：US364665
申请日：1982-04-02
申请人： Rolf Zander , Hans U. Wolf
发明人： Rolf Zander , Hans U. Wolf
IPC分类号： G01N21/80 , G01N33/487 , G01N33/49 , G01N33/72 , G01N33/84
CPC分类号： G01N33/4925 , G01N33/84 , Y10T436/204998
摘要： In order to simplify the operation for determination of the acid-base status of blood, composed of the values for pH, pCO.sub.2 and the base excess BE, the base excess BE is determined through measurement of the pH-value at a pCO.sub.2 of about 0 mm Hg. The pH-values may be determined photometrically, in which case the indicator solution for measuring the actual blood-pH is an aqueous solution of 40 micromol/l bromthymol blue plus 0.2 g/l sodium dodecylsulfate plus 1% ethanol; and for measurement of the base excess BE is an aqueous solution of 65 micro mol/l naphtholphthalein plus 0.2 g/l sodium dodecylsulfate plus 15% dimethylsulfoxide, which are measured at a wavelength of 635 nm. If desired, one may use a single solution for measuring both actual blood-pH as well as the base excess BE, composed of 16 micromol/l bromthymol blue plus 34 micromol/l naphtholphthalein plus 0.15 g/l sodium dodecylsulfate, which is measured at 615 nm wavelength.
摘要翻译：为了简化用于测定pH值，pCO 2和碱过量BE的血液酸碱状态的操作，通过测定pCO 2的pH值约为0来测定碱过量BE mm Hg。 pH值可以光度测定，在这种情况下，用于测量实际血液pH值的指示剂溶液是40微摩尔/升溴百里酚蓝加0.2克/升十二烷基硫酸钠加1％乙醇的水溶液; 并且对于碱过量的测量，BE是在635nm波长下测量的65微摩尔/升萘并噻吩加0.2克/升十二烷基硫酸钠加上15％二甲基亚砜的水溶液。如果需要，可以使用单一溶液来测量实际的血液pH值以及由16微摩尔/升溴百里酚蓝加上34微摩尔/萘酚酞加0.15克/升十二烷基硫酸钠组成的碱过量的BE，其在 615 nm波长。

3. 发明授权

US4341527A Process and reagent for determination of the hemoglobin content of blood 失效
标题翻译：用于测定血液中血红蛋白含量的方法和试剂
公开(公告)号：US4341527A
公开(公告)日：1982-07-27
申请号：US170575
申请日：1980-07-21
申请人： Rolf Zander , Werner Lang , Hans U. Wolf
发明人： Rolf Zander , Werner Lang , Hans U. Wolf
IPC分类号： B01F20060101 , C11D20060101 , G01N20060101 , G01N33/72
CPC分类号： G01N33/721
摘要： A reagent for photometric determination of the hemoglobin content of blood, which produces a characteristic color upon addition of the blood sample, consisting of an aqueous alkaline solution comprising a water-soluble, non-ionic detergent, whereby all hemoglobin-derivatives occurring in the blood and hemederivatives are converted into a unitary end product having a distinct absorption maximum in the visible spectral range.
摘要翻译：用于光度测定血液中血红蛋白含量的试剂，其在加入血液样品时产生特征性颜色，其由包含水溶性非离子型洗涤剂的碱性水溶液组成，由此血液中存在所有血红蛋白衍生物并且将中间体转化为在可见光谱范围内具有明显吸收最大值的单一最终产物。

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